LOCUS       CK580-_PB-cT3G-MG-Barco 7180 bp ds-DNA     circular     28-DEC-2022
DEFINITION  .
COMMENT     an expression clone created from CK530- PB-cT3G-MG-Barcoded-cERP2
            and CK475- pDONOR221 - FIGLAan expression clone created from CK530-
            PB-cT3G-MG-Barcoded-cERP2  and CK475- pDONOR221 - FIGLA
FEATURES             Location/Qualifiers
     primer_bind     1..17
                     /label="M13R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: common sequencing primer, one of multiple similar variants locus_tag: M13 rev ApEinfo_label: M13 rev ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind Sequence: CAGGAAACAGCTATGAC Hairpin Tm: None Tm: 49.1 Self Dimer Tm: None %GC: 47.1 created by: primer3 modified by: wyssuser"
     repeat_region   43..105
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: piggyBac transposon-specific inverted terminal repeat sequence (ITR) locus_tag: piggyBac right (3') inverted repeat ApEinfo_label: piggyBac right (3') inverted repeat ApEinfo_fwdcolor: #0dfff7 ApEinfo_revcolor: #0dfff7 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: repeat_region"
     polyA_signal    281..362
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: SV40 polyadenylation signal locus_tag: SV40 poly(A) signal(1) ApEinfo_label: SV40 poly(A) signal ApEinfo_fwdcolor: #ff3eee ApEinfo_revcolor: #ff3eee ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: polyA_signal modified by: wyssuser"
     primer_bind     389..408
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: GTTTCAGGTTCAGGGGGAGG Hairpin Tm: None Tm: 60.0 Self Dimer Tm: None %GC: 60.0 modified by: wyssuser"
     CDS             complement(479..1078)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created by: wyssuser"
     primer_bind     964..983
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="# Off-target Sites: 0 Off-target Template: Final Plasmid Sequence: GATCTACGGTATGGCGGGTC Hairpin Tm: None Tm: 59.8 Self Dimer Tm: None %GC: 60.0 Pair Dimer Tm: None Product Size: 35 annotation group: 69733439644894ee5c5e1-3592-4568-a0da-b1c47594b8cdFinal Plasmid modified by: wyssuser"
     CDS             complement(1079..1135)
                     /label="P2A"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="codon_start: 1 product: 2A peptide from porcine teschovirus-1 polyprotein note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. translation: ATNFSLLKQAGDVEENPGP locus_tag: P2A ApEinfo_label: P2A ApEinfo_fwdcolor: #e9d024 ApEinfo_revcolor: #e9d024 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: CDS"
     CDS             complement(1145..1888)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="created by: wyssuser"
     primer_bind     complement(1286..1305)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: GTGGCCTGGAGAAACAGCTA Hairpin Tm: 37.2 Tm: 59.7 Self Dimer Tm: 3.3 %GC: 55.0 modified by: wyssuser"
     primer_bind     1531..1550
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: GGAACGCGAGCTGATTTTCC Hairpin Tm: None Tm: 59.9 Self Dimer Tm: 15.9 %GC: 55.0 modified by: wyssuser"
     CDS             complement(1889..1912)
                     /label="FLAG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 product: FLAG(R) epitope tag, followed by an enterokinasecleavage site translation: DYKDDDDK locus_tag: FLAG ApEinfo_label: FLAG ApEinfo_fwdcolor: #e9d024 ApEinfo_revcolor: #e9d024 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: CDS"
     regulatory      complement(1912..1921)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="regulatory_class: other note: vertebrate consensus sequence for strong initiationof translation (Kozak, 1987) locus_tag: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) ApEinfo_label: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: regulatory modified by: wyssuser"
     promoter        complement(1941..2152)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="note: core promoter for human elongation factor EF-1-alpha locus_tag: EF-1-alpha core promoter ApEinfo_label: EF-1-alpha core promoter ApEinfo_fwdcolor: #346ee0 ApEinfo_revcolor: #346ee0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: promoter"
     regulatory      complement(2153..2532)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="created by: wyssuser modified by: wyssuser"
     primer_bind     2681..2700
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="# Off-target Sites: 0 Off-target Template: Final Plasmid Sequence: TTTGAGTAGCGACTGACGGG annotation group: 646536956143517519a63-5614-47f8-a332-a32195d8b3a1Final Plasmid modified by: wyssuser Hairpin Tm: 42.8 Tm: 59.8 Self Dimer Tm: 3.5 %GC: 55.0 Pair Dimer Tm: None Product Size: 614"
     extracted re... 2692..2724
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Extracted interval: 5833 -> 5865 Original Circular Sequence Length: 5865"
     extracted re... 2725..2757
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="Extracted interval: 6503 -> 6535 Original Circular Sequence Length: 6535"
     primer_bind     complement(2734..2752)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: CACTCGAGTGGCTCCGGTG Hairpin Tm: 42.1 Tm: 62.6 Self Dimer Tm: 33.4 %GC: 68.4 modified by: wyssuser"
     extracted re... 2758..3132
                     /label="Extracted region from CK530- PB-cT3G-MG-Barcoded-cERP2 "
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Extracted interval: 1 -> 375 Original Circular Sequence Length: 6831"
     promoter        2766..3021
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="created by: wyssuser"
     protein_bind    2774..2792
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    2810..2828
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(1) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    2846..2864
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(2) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    2882..2900
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(3) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    2918..2936
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(4) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    2954..2972
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(5) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    2990..3008
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(6) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     promoter        3022..3121
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="created by: wyssuser"
     protein_bind    3125..3132
                     /label="attR1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="bound_moiety: <html><body>LR Clonase™</body></html> gene: <html><body>mutant version of&nbsp;<i>att</i>R</body></html> note: <html><body>recombination site for the Gateway® LR reaction</body></html>"
     attB1           3126..3151
                     /label="attB1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="annotation group: attB"
     extracted re... 3133..3151
                     /label="Extracted region from null"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Extracted interval: 9 -> 27 Original Circular Sequence Length: 3213"
     misc_feature    3152..3157
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="created by: wyssuser"
     extracted re... 3152..3817
                     /label="Extracted region from FIGLA_gBlock with attB sites"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Extracted interval: 32 -> 697 Original Circular Sequence Length: 3213"
     CDS             3158..3814
                     /label="FIGLA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="created by: wyssuser"
     Editing Hist... 3815..3817
                     /label="misc_feature"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 3818..3828
                     /label="Extracted region from attB2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="Extracted interval: 16 <- 26 Original Circular Sequence Length: 3213"
     attB2           complement(3818..3842)
                     /label="attB2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="annotation group: attB"
     protein_bind    complement(3829..3843)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="bound_moiety: <html><body>LR Clonase™</body></html> gene: <html><body>mutant version of <i>att</i>R</body></html> note: <html><body>recombination site for the Gateway® LR reaction</body></html>"
     extracted re... 3829..3906
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Extracted interval: 723 -> 800 Original Circular Sequence Length: 6786"
     CDS             3845..3871
                     /label="HA Tag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="created by: wyssuser"
     terminator      3872..3880
                     /label="STOPS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="organism: synthetic DNA construct mol_type: other DNA locus_tag: source:synthetic DNA construct(4) ApEinfo_label: source:synthetic DNA construct ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: source modified by: wyssuser"
     primer_bind     3884..3906
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Sequence: ATACTCAGAAGATGTCACTCACC Hairpin Tm: None Tm: 57.4 Self Dimer Tm: None %GC: 43.5 created by: primer3 .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     primer_bind     3884..3906
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="Sequence: ATACTCAGAAGATGTCACTCACC Hairpin Tm: None Tm: 57.4 Self Dimer Tm: None %GC: 43.5 created by: primer3 Extension: ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     extracted re... 3907..3976
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Extracted interval: 8 -> 77 Original Circular Sequence Length: 6831"
     misc_difference 3911..3933
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="created by: wyssuser"
     misc_feature    3934..3953
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="created by: wyssuser modified by: wyssuser"
     misc_difference complement(3954..3976)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="created by: wyssuser modified by: wyssuser"
     extracted re... 3977..3980
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Extracted interval: 223 -> 226 Original Circular Sequence Length: 6769"
     primer_bind     complement(3981..4003)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Sequence: CATTCATAGTTCTTGCTCAGTGG Hairpin Tm: None Tm: 57.5 Self Dimer Tm: None %GC: 43.5 created by: primer3 Extension: GAGTTCAGACGTGTGCTCTTCCGATCTNNN .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     primer_bind     complement(3981..4003)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Sequence: CATTCATAGTTCTTGCTCAGTGG Hairpin Tm: None Tm: 57.5 Self Dimer Tm: None %GC: 43.5 created by: primer3 .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     extracted re... 3981..4012
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Extracted interval: -8 <- 23 Original Circular Sequence Length: 6769"
     motif           4004..4028
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="created by: wyssuser modified by: wyssuser"
     extracted re... 4013..4046
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Extracted interval: 259 -> 292 Original Circular Sequence Length: 6769"
     polyA_signal    4038..4172
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="note: SV40 polyadenylation signal locus_tag: SV40 poly(A) signal ApEinfo_label: SV40 poly(A) signal ApEinfo_fwdcolor: #ff3eee ApEinfo_revcolor: #ff3eee ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: polyA_signal modified by: wyssuser"
     extracted re... 4047..4202
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Extracted interval: 636 -> 791 Original Circular Sequence Length: 6529"
     extracted re... 4203..6913
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="Extracted interval: 1035 -> 3745 Original Circular Sequence Length: 6769"
     repeat_region   complement(4444..4478)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: piggyBac transposon-specific inverted terminal repeat sequence (ITR) locus_tag: piggyBac left (5') inverted repeat ApEinfo_label: piggyBac left (5') inverted repeat ApEinfo_fwdcolor: #0dfff7 ApEinfo_revcolor: #0dfff7 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: repeat_region modified by: wyssuser"
     primer_bind     complement(4490..4505)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="Sequence: GTAAAACGACGGCCAG Hairpin Tm: None Tm: 52.4 Self Dimer Tm: 2.2 %GC: 56.3 created by: primer3"
     primer_bind     complement(4714..4733)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: pRS vectors, use to sequence yeast selectable marker locus_tag: pRS-marker ApEinfo_label: pRS-marker ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     primer_bind     4835..4857
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="note: pGEX vectors, reverse primer locus_tag: pGEX 3' ApEinfo_label: pGEX 3' ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     primer_bind     complement(4896..4914)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="note: pBR322 vectors, upsteam of EcoRI site, forward primer locus_tag: pBRforEco ApEinfo_label: pBRforEco ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     promoter        4982..5086
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="gene: bla locus_tag: AmpR promoter ApEinfo_label: AmpR promoter ApEinfo_fwdcolor: #346ee0 ApEinfo_revcolor: #346ee0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: promoter"
     CDS             5087..5947
                     /label="AmpR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 gene: bla product: beta-lactamase note: confers resistance to ampicillin, carbenicillin, and related antibiotics translation: MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW locus_tag: AmpR ApEinfo_label: AmpR ApEinfo_fwdcolor: #e9d024 ApEinfo_revcolor: #e9d024 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: CDS"
     primer_bind     complement(5305..5324)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="note: Ampicillin resistance gene, reverse primer locus_tag: Amp-R ApEinfo_label: Amp-R ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     rep_origin      6118..6706
                     /label="ori"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication locus_tag: ori ApEinfo_label: ori ApEinfo_fwdcolor: #999999 ApEinfo_revcolor: #999999 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: rep_origin"
     primer_bind     6607..6626
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: pBR322 origin, forward primer locus_tag: pBR322ori-F ApEinfo_label: pBR322ori-F ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     primer_bind     6860..6877
                     /label="L4440"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: L4440 vector, forward primer locus_tag: L4440 ApEinfo_label: L4440 ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     extracted re... 6914..7002
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Extracted interval: 278 -> 366 Original Circular Sequence Length: 6769"
     extracted re... 7003..2691
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Extracted interval: 3835 -> 6703 Original Circular Sequence Length: 6769"
     protein_bind    7083..7104
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presenceof cAMP. locus_tag: CAP binding site ApEinfo_label: CAP binding site ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind"
     promoter        7119..7149
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon locus_tag: lac promoter ApEinfo_label: lac promoter ApEinfo_fwdcolor: #346ee0 ApEinfo_revcolor: #346ee0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: promoter"
     protein_bind    7157..7173
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). locus_tag: lac operator ApEinfo_label: lac operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind"
ORIGIN
        1 CAGGAAACAG CTATGACCAT GATTACGCCA AGGTCGACTT AACCCTAGAA AGATAATCAT
       61 ATTGTGACGT ACGTTAAAGA TAATCATGCG TAAAATTGAC GCATGTGTTT TATCGGTCTG
      121 TATATCGAGG TTTATTTATT AATTTGAATA GATATTAAGT TTTATTATAT TTACACTTAC
      181 ATACTAATAA TAAATTCAAC AAACAATTTA TTTATGTTTA TTTATTTATT AAAAAAAAAC
      241 AAAAACTCAA AATTTCTTCT ATAAAGTAAC AAAACTTTTA GCAGTGAAAA AAATGCTTTA
      301 TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG
      361 TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT
      421 TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCCT CTGGAGATTC
      481 ATGCGCCAGG TTTTCTGGTC ATGCACCACG TTCGTGGGCC CTCAGGAACC TCCACGTCAG
      541 CGGTAACTGT GAAGCCCAGT CGCTCGTAAA AAGGAAGATT CCGGGGAGCA CTGGTTTCGA
      601 GGAACGCCGG CACGCCAGCC CGTTCCGCGG CCTCAACACC AGGAAGAACG ACAGCGCTTC
      661 CCAAACCTTT ACCTTGGTGA TCTGGGCTGA CACCGACAGT GGCAAGAAAC CACGCAGGCT
      721 CCTTTGGTCT ATGAGGAGCC AGCAAGCCCT CCATTTGCTG TTGTGCCGCC AACCTACTCC
      781 CTGACAGCTC GGCCATCCGC GGACCTATTT CCGCGAACAC TGCGCCGGCT TCGACGCTTT
      841 CCGGAGTAGT CCAGACCGCA ACTGCGGCAC CATCGTCCGC AACCCAAACT TTACCAATGT
      901 CCAGACCCAC GCGCGTAAGA AACAGCTCTT GCAACTCAGT TACCCTCTCT ATGTGTCTGT
      961 CCGGATCTAC GGTATGGCGG GTCGCTGGAT AATCTGCAAA TGCTGCGGCA AGTGTTCGGA
     1021 CAGCCCGTGG TACATCGTCA CGGGTAGCGA GCCTAACAGT GGGCTTATAT TCCGTCATGG
     1081 GGCCGGGGTT CTCCTCCACG TCGCCGGCCT GCTTCAGCAG GCTGAAGTTG GTGGCGCCGC
     1141 TGCCCCCGGG GAGCATGTCA AGGTCAAAAT CGTCAAGAGC GTCAGCAGGC AGCATATCAA
     1201 GGTCAAAGTC GTCAAGGGCA TCGGCTGGGA GCATGTCTAA GTCAAAATCG TCAAGGGCGT
     1261 CGGTCGGCCC GCCGCTTTCG CACTTTAGCT GTTTCTCCAG GCCACATATG ATTAGTTCCA
     1321 GGCCGAAAAG GAAGGCAGGT TCGGCTCCCT GCCGGTCGAA CAGCTCAATT GCTTGTTTCA
     1381 GAAGTGGGGG CATAGAATCG GTGGTAGGTG TCTCTCTTTC CTCTTTTGCT ACTTGATGCT
     1441 CCTGTTCCTC CAATACGCAG CCCAGTGTAA AGTGGCCCAC GGCGGACAGA GCGTACAGTG
     1501 CGTTCTCCAG GGAGAAGCCT TGCTGACACA GGAACGCGAG CTGATTTTCC AGGGTTTCGT
     1561 ACTGTTTCTC TGTTGGGCGG GTGCCGAGAT GCACTTTAGC CCCGTCACGA TGTGAGAGGA
     1621 GAGCACAGCG GTATGACTTG GCGTTGTTCC GCAGAAAGTC TTGCCATGAC TCGCCTTCCA
     1681 GGGGGCAGGA GTGGGTATGA TGCCTGTCCA GCATCTCGAT TGGCAGGGCA TCGAGCAGGG
     1741 CCCGCTTGTT CTTCACGTGC CAGTACAGGG TAGGCTGCTC AACTCCCAGC TTTTGAGCGA
     1801 GTTTCCTTGT CGTCAGGCCT TCGATACCGA CTCCATTGAG TAATTCCAGA GCAGAGTTTA
     1861 TGACTTTGCT CTTGTCCAGT CTAGACATCT TATCGTCATC GTCTTTGTAA TCCATGGTGG
     1921 CGGATCCCGC GTCACGACAC CTGTGTTCTG GCGGCAAACC CGTTGCGAAA AAGAACGTTC
     1981 ACGGCGACTA CTGCACTTAT ATACGGTTCT CCCCCACCCT CGGGAAAAAG GCGGAGCCAG
     2041 TACACGACAT CACTTTCCCA GTTTACCCCG CGCCACCTTC TCTAGGCACC GGTTCAATTG
     2101 CCGACCCCTC CCCCCAACTT CTCGGGCACT GTGGGCCATG TGCGCTCTGC CCCATGGTAA
     2161 TAGCCATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA GTGCCATAAG GTCATGTACT
     2221 GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA
     2281 TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG TAAATACTCC ACCCATTGAC
     2341 GTCAATGGAA AGTGCCTATT GGCGTTACTA TGGGAACATA CGTCATTATT GACGTCAATG
     2401 GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC CGTAAGTTAT GTAACGCGGA
     2461 ACTCCATATA TGGGCTATGA ACTAATGACC CCGTAATTGA TTACTATTAA TAACTAGTCA
     2521 ATAATCAATG TCATAAGCTC TAATCGCATA ATCGAGCTAA GAGCGATTAA TATGCTTAGC
     2581 TAGCTTAGCT AAGCTAGATT TCTCTATTCT CCCACAATAT ATAGCAGCTA TTAGCTTAGC
     2641 AGTCCGAATA CGATCGTCCA CGATCCATAA GCGTAACACA TTTGAGTAGC GACTGACGGG
     2701 CACCGGAGCC ACTCGAGTGG AATTACTGAC GGGCACCGGA GCCACTCGAG TGGAATTATC
     2761 ACCTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGA AGAGTTTACT CCCTATCAGT
     2821 GATAGAGAAC GTATGCAGAC TTTACTCCCT ATCAGTGATA GAGAACGTAT AAGGAGTTTA
     2881 CTCCCTATCA GTGATAGAGA ACGTATGACC AGTTTACTCC CTATCAGTGA TAGAGAACGT
     2941 ATCTACAGTT TACTCCCTAT CAGTGATAGA GAACGTATAT CCAGTTTACT CCCTATCAGT
     3001 GATAGAGAAC GTATAAGCTT GCCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG
     3061 CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGGGAC CGATCCAGCC
     3121 TACCACAAGT TTGTACAAAA AAGCAGGCTT AGCCACCATG GACCCCGCGC CCGGCGTCCT
     3181 AGATCCACGT GCTGCACCAC CAGCACTTCT AGGTACTCCA CAAGCAGAAG TACTAGAAGA
     3241 TGTATTACGA GAACAATTTG GACCGCTGCC CCAGCTGGCC GCTGTCTGCC GGCTCAAGCG
     3301 GCTGCCCTCG GGCGGCTACT CGTCCACTGA AAACCTCCAG TTGGTGCTGG AGCGGCGGCG
     3361 TGTGGCCAAC GCCAAGGAGC GTGAGCGGAT AAAAAATCTC AACCGTGGTT TTGCCAGATT
     3421 GAAGGCACTT GTGCCATTTC TTCCCCAAAG CAGGAAGCCC AGCAAAGTTG ATATCCTTAA
     3481 AGGTGCGACT GAATATATAC AGGTTCTCAG TGATCTTTTG GAAGGAGCCA AAGACTCAAA
     3541 GAAACAAGAC CCAGATGAGC AGAGCTATAG TAACAACAGT TCTGAATCAC ATACATCCTC
     3601 GGCAAGACAG CTGTCAAGAA ACATCACCCA ACATATCAGC TGTGCTTTCG GCTTGAAGAA
     3661 TGAAGAGGAA GGGCCTTGGG CAGATGGTGG CAGTGGTGAG CCAGCACACG CTTGTCGCCA
     3721 CAGTGTGATG TCTACGACTG AAATTATCTC CCCAACCAGA AGTCTGGATA GATTCCCAGA
     3781 AGTAGAACTG CTGAGTCACA GACTTCCACA AGTATAATAC CCAGCTTTCT TGTACAAAGT
     3841 GGTATACCCA TACGATGTTC CAGATTACGC TTAGTAATGA GAAATACTCA GAAGATGTCA
     3901 CTCACCATAG TCCGCAGTGA AAGATAAATG ATCNNNNNNN NNNNNNNNNN NNNGTTTTAG
     3961 AGCTAGAAAT AGCAAGTAGG CCACTGAGCA AGAACTATGA ATGAGATCTT TAGACATATT
     4021 GCGGCCGCTT TCTCCGCGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA
     4081 AAACTAGAAT GCAGTGAAAA AAATGCCTTA TTTGTGAAAT TTGTGATGCT ATTGCCTTAT
     4141 TTGTAACCAT TATAAGCTGC AATAAACAAG TTATCTATAA CAAGAAAATA TATATATAAT
     4201 AAGTTATCAC GTAAGTAGAA CATGAAATAA CAATATAATT ATCGTATGAG TTAAATCTTA
     4261 AAAGTCACGT AAAAGATAAT CATGCGTCAT TTTGACTCAC GCGGTCGTTA TAGTTCAAAA
     4321 TCAGTGACAC TTACCGCATT GACAAGCACG CCTCACGGGA GCTCCAAGCG GCGACTGAGA
     4381 TGTCCTAAAT GCACAGCGAC GGATTCGCGC TATTTAGAAA GAGAGAGCAA TATTTCAAGA
     4441 ATGCATGCGT CAATTTTACG CAGACTATCT TTCTAGGGTT AAGAATTCAC TGGCCGTCGT
     4501 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA
     4561 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA
     4621 GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG
     4681 CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT
     4741 AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC
     4801 GGCATCCGCT TACAGACAAG CTGTGACCAT GTCTCCGGGA GCTGCATGTG TCAGAGGTTT
     4861 TCACCGTCAT CACCGAAACA CGCGAGTTCG AAAGGGCCTC GTGATACGCC TATTTTTATA
     4921 GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT
     4981 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
     5041 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA
     5101 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC
     5161 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT
     5221 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC
     5281 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG
     5341 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC
     5401 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT
     5461 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA
     5521 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC
     5581 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC
     5641 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT
     5701 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC
     5761 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGTTCACGCG GTATCATTGC
     5821 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA
     5881 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA
     5941 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT
     6001 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA
     6061 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG
     6121 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC
     6181 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG
     6241 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA
     6301 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC
     6361 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC
     6421 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA
     6481 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG
     6541 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT
     6601 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA
     6661 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC
     6721 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT
     6781 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG
     6841 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGATGAGC GCCCAATACG
     6901 CAAACCGCCT CTCACCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC
     6961 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT
     7021 CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA
     7081 ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT
     7141 CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA
//