LOCUS       pWXC077_mScarlet        4409 bp DNA     circular SYN 18-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2024 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_75
FEATURES             Location/Qualifiers
     source          1..4409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          72..113
                     /label=artificial intron
     CDS             join(144..293,345..668,720..935)
                     /codon_start=1
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     3'UTR           1072..1799
                     /label=unc-54 3'UTR
     primer_bind     complement(1859..1881)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1981..2000
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2078..2096)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2164..2268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2269..2337,2338..3129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2487..2506)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3300..3888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3789..3808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4042..4059
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4176..4197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4212..4217,4218..4235,4236..4242)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4250..4266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4255..4277
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4274..4290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4274..4290
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaaatgaaat aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa
       61 aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc
      121 cttggagggt accggtagaa aaaatggact ccaccgaggc cgtcatcaag gagttcatgc
      181 gtttcaaggt ccacatggag ggatccatga acggacacga gttcgagatc gagggagagg
      241 gagagggacg tccatacgag ggaacccaaa ccgccaagct ccgtgtcacc aaggtaagtt
      301 taaacatata tatactaact aaccctgatt atttaaattt tcagggagga ccactcccat
      361 tctcctggga catcctctcc ccacaattca tgtacggatc ccgtgccttc accaagcacc
      421 cagccgacat cccagactac tggaagcaat ccttcccaga gggattcaag tgggagcgtg
      481 tcatgaactt cgaggacgga ggagccgtct ccgtcgccca agacacctcc ctcgaggacg
      541 gaaccctcat ctacaaggtc aagctccgtg gaaccaactt cccaccagac ggaccagtca
      601 tgcaaaagaa gaccatggga tgggaggcct ccaccgagcg tctctaccca gaggacgtcg
      661 tcctcaaggt aagtttaaac agttcggtac taactaacca tacatattta aattttcagg
      721 gagacatcaa gatggccctc cgtctcaagg acggaggacg ttacctcgcc gacttcaaga
      781 ccacctaccg tgccaagaag ccagtccaaa tgccaggagc cttcaacatc gaccgtaagc
      841 tcgacatcac ctcccacaac gaggactaca ccgtcgtcga gcaatacgag cgttccgtcg
      901 cccgtcactc caccggagga tccggaggat cctaacattc gtagaattcc aactgagcgc
      961 cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga
     1021 gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg
     1081 cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc
     1141 tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt
     1201 tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt
     1261 cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca
     1321 aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg
     1381 aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg
     1441 accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg
     1501 tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat
     1561 tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt
     1621 ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt
     1681 tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc
     1741 tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc
     1801 ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     1861 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     1921 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     1981 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     2041 gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta
     2101 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     2161 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2221 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2281 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2341 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2401 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2461 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2521 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2581 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2641 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2701 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2761 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2821 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2881 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2941 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3001 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3061 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3121 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3181 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3241 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3301 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3361 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3421 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3481 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3541 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3601 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3661 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     3721 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3781 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3841 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3901 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3961 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4021 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     4201 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4261 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctgta agtttaaaca
     4321 tgatcttact aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca
     4381 ctcacaacga tggatacgct aacaacttg
//
