LOCUS       40651-EFSNS-FMR1_e1-24x 7744 bp ds-DNA     circular     23-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          35..74
                     /label="8H5"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        53..308
                     /label="EFS-NS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    155..155
                     /label="mis-match A=>T"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    275..307
                     /label="exon 1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(287..307)
                     /label="8H6"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          289..328
                     /label="8H7"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          309..319
                     /label="CGG-F2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     exon            320..631
                     /label=1
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    420..511
                     /label="CGG\repeats"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          528..547
                     /label="3F8"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             581..631
                     /label="CDS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(611..655)
                     /label="8H8"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    636..636
                     /label="missing in Sanger"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    646..1180
                     /label="confirmed by Sanger"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    646..1947
                     /label="24xMS2?"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    660..678
                     /label="MS2 stem loop"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1971..2546
                     /label="WPRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2036..2055)
                     /label="WPRE-seqR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2615..2630
                     /label="cPPT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2615..2636
                     /label="U3PPT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2632..2684
                     /label="delta_U3"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2685..2865
                     /label="truncHIV-1_3_LTR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        3105..3133
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     gene            3175..4035
                     /label="Ampicillin gene"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      4190..4809
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        5241..5270
                     /label="lac_promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    6033..6213
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    6324..6368
                     /label="HIV-1_psi_pack"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    6882..7115
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    7641..7656
                     /label="cPPT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta gttaggtctt
       61 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
      121 ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg
      181 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      241 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      301 aacacagggt ttcggtttca cttccggtgg agggccgcct ctgagcgggc ggcgggccga
      361 cggcgagcgc gggcggcggc ggtgacggag gcgccgctgc cagggggcgt gcggcagcgc
      421 ggcGCGGCGG CGGCGGCGGC GGCGGCGGAG GCggcggcgg cggcggcggc ggcggcggag
      481 gcggcggcgg cggcggcggc ggcggcggcg gctgggcctc gagcgcccgc agcccacctc
      541 tcgggggcgg gctcccggcg ctagcagggc tgaagagaag atggaggagc tggtggtgga
      601 agtgcggggc tccaatggcg ctttctacaa ggatcctaag gtacctaatt gcctagaaaa
      661 catgaggatc acccatgtct gcaggtcgac tctagaaaac atgaggatca cccatgtctg
      721 cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaaac atgaggatca
      781 cccatgtctg caggtcgact ctagaaaaca tgaggatcac ccatgtctgc agtattcccg
      841 ggttcattag atcctaaggt acctaattgc ctagaaaaca tgaggatcac ccatgtctgc
      901 aggtcgactc cagaaaacat gaggatcacc catgtctgca gtattcccgg gttcattaga
      961 tcctaaggta cctaattgcc tagaaaacat gaggatcacc catgtctgca ggtcgactct
     1021 agaaaacatg aggatcaccc atgtctgcag tattcccggg ttcattagat cctaaggtac
     1081 ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcta gaaaacatga
     1141 ggatcaccca tgtctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta
     1201 gaaaacatga ggatcaccca tgtctgcagg tcgactccag aaaacatgag gatcacccat
     1261 gtctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaacatgag
     1321 gatcacccat gtctgcaggt cgactctaga aaacatgagg atcacccatg tctgcagtat
     1381 tcccgggttc attagatcct aaggtaccta attgcctaga aaacatgagg atcacccatg
     1441 tctgcaggtc gactctagaa aacatgagag gatcacccat gtctgcagta ttcccgggtt
     1501 cattagatcc taaggtacct aattgcctag aaaacatgag gatcacccat gtctgcaggt
     1561 cgactctaga aaacatgagg atcacccatg tctgcagtat tcccgggttc attagatcct
     1621 aaggtaccta attgcctaga aaacatgagg atcacccatg tctgcaggtc gactctagaa
     1681 aacatgagga tcacccatgt ctgcagtatt cccgggttca ttagatccta aggtacctaa
     1741 ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg actctagaaa acatgaggat
     1801 cacccatgtc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
     1861 acatgaggat cacccatgtc tgcaggtcgg actctagaaa acatgaggat cacccatgtc
     1921 tgcagtattc ccgggttcat tagatctgcc gcgcgatcga tagatcctaa tcaacctctg
     1981 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2041 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2101 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2161 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2221 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2281 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2341 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     2401 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2461 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     2521 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
     2581 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     2641 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     2701 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     2761 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     2821 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     2881 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
     2941 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
     3001 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3061 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3121 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3181 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3241 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3301 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3361 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     3421 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3481 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3541 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3601 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3661 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3721 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3781 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3841 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3901 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3961 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4021 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4081 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4141 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4201 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4261 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4321 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4381 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4441 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4501 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4561 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4621 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4681 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4741 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4801 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4861 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4921 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4981 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
     5041 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5101 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
     5161 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     5221 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     5281 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
     5341 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5401 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     5461 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     5521 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     5581 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     5641 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     5701 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     5761 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     5821 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     5881 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     5941 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     6001 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     6061 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6121 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6181 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     6241 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     6301 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     6361 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     6421 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     6481 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     6541 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     6601 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     6661 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     6721 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     6781 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     6841 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     6901 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     6961 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7021 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7081 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7141 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     7201 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7261 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7321 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7381 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7441 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7501 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     7561 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg
     7621 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     7681 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     7741 aaat
//