LOCUS       p40651-EFSNS-FMR1_e1ΔCG 7652 bp ds-DNA     circular     18-JUN-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          35..74
                     /label="8H5"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        53..308
                     /label="EFS-NS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    155..155
                     /label="mis-match A=>T"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    275..307
                     /label="exon 1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(287..307)
                     /label="8H6"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          289..328
                     /label="8H7"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          309..319
                     /label="CGG-F2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     exon            320..539
                     /label=1
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          436..455
                     /label="3F8"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             489..539
                     /label="CDS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(519..563)
                     /label="8H8"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    544..544
                     /label="missing in Sanger"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    554..1088
                     /label="confirmed by Sanger"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    554..1855
                     /label="24xMS2?"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    568..586
                     /label="MS2 stem loop"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1879..2454
                     /label="WPRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1944..1963)
                     /label="WPRE-seqR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2523..2538
                     /label="cPPT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2523..2544
                     /label="U3PPT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2540..2592
                     /label="delta_U3"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2593..2773
                     /label="truncHIV-1_3_LTR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        3013..3041
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     gene            3083..3943
                     /label="Ampicillin gene"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      4098..4717
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        5149..5178
                     /label="lac_promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5941..6121
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    6232..6276
                     /label="HIV-1_psi_pack"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    6790..7023
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    7549..7564
                     /label="cPPT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta gttaggtctt
       61 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
      121 ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg
      181 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      241 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      301 aacacagggt ttcggtttca cttccggtgg agggccgcct ctgagcgggc ggcgggccga
      361 cggcgagcgc gggcggcggc ggtgacggag gcgccgctgc cagggggcgt gcggcagcgc
      421 tgggcctcga gcgcccgcag cccacctctc gggggcgggc tcccggcgct agcagggctg
      481 aagagaagat ggaggagctg gtggtggaag tgcggggctc caatggcgct ttctacaagg
      541 atcctaaggt acctaattgc ctagaaaaca tgaggatcac ccatgtctgc aggtcgactc
      601 tagaaaacat gaggatcacc catgtctgca gtattcccgg gttcattaga tcctaaggta
      661 cctaattgcc tagaaaacat gaggatcacc catgtctgca ggtcgactct agaaaacatg
      721 aggatcaccc atgtctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct
      781 agaaaacatg aggatcaccc atgtctgcag gtcgactcca gaaaacatga ggatcaccca
      841 tgtctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaacatga
      901 ggatcaccca tgtctgcagg tcgactctag aaaacatgag gatcacccat gtctgcagta
      961 ttcccgggtt cattagatcc taaggtacct aattgcctag aaaacatgag gatcacccat
     1021 gtctgcaggt cgactctaga aaacatgagg atcacccatg tctgcagtat tcccgggttc
     1081 attagatcct aaggtaccta attgcctaga aaacatgagg atcacccatg tctgcaggtc
     1141 gactccagaa aacatgagga tcacccatgt ctgcagtatt cccgggttca ttagatccta
     1201 aggtacctaa ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg actctagaaa
     1261 acatgaggat cacccatgtc tgcagtattc ccgggttcat tagatcctaa ggtacctaat
     1321 tgcctagaaa acatgaggat cacccatgtc tgcaggtcga ctctagaaaa catgagagga
     1381 tcacccatgt ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa
     1441 aacatgagga tcacccatgt ctgcaggtcg actctagaaa acatgaggat cacccatgtc
     1501 tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa acatgaggat
     1561 cacccatgtc tgcaggtcga ctctagaaaa catgaggatc acccatgtct gcagtattcc
     1621 cgggttcatt agatcctaag gtacctaatt gcctagaaaa catgaggatc acccatgtct
     1681 gcaggtcgac tctagaaaac atgaggatca cccatgtctg cagtattccc gggttcatta
     1741 gatcctaagg tacctaattg cctagaaaac atgaggatca cccatgtctg caggtcggac
     1801 tctagaaaac atgaggatca cccatgtctg cagtattccc gggttcatta gatctgccgc
     1861 gcgatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     1921 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     1981 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2041 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2101 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2161 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2221 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     2281 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     2341 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     2401 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     2461 cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2521 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     2581 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2641 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2701 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2761 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     2821 aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg
     2881 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2941 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3001 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3061 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3121 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3181 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3241 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3301 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     3361 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3421 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3481 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3541 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3601 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3661 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3721 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3781 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3841 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3901 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3961 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4021 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4081 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4141 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4201 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4261 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4321 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4381 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4441 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4501 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4561 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4621 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4681 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4741 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4801 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4861 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4921 gccgattcat taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc
     4981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5041 ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga
     5101 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5161 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5221 ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5281 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     5341 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     5401 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     5461 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     5521 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     5581 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     5641 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     5701 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     5761 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     5821 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     5881 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     5941 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6001 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6061 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6121 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     6181 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     6241 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     6301 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     6361 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     6421 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     6481 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     6541 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     6601 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     6661 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     6721 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     6781 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     6841 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     6901 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     6961 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     7021 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     7081 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     7141 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     7201 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     7261 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     7321 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     7381 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     7441 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     7501 tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg
     7561 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     7621 actaaagaat tacaaaaaca aattacaaaa at
//