LOCUS       His_CL7_NLS_Cas9        8647 bp DNA     circular SYN 06-MAY-2024
DEFINITION  obtaining full Cas9 RNP.
ACCESSION   .
VERSION     .
KEYWORDS    pCold CL7-Cas9-A22p3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Qiao J, Li W, Lin S, Sun W, Ma L, Liu Y
  TITLE     Co-expression of Cas9 and single-guided RNAs in Escherichia coli 
            streamlines production of Cas9 ribonucleoproteins.
  JOURNAL   Commun Biol. 2019 May 3;2:161. doi: 10.1038/s42003-019-0402-x. 
            eCollection 2019.
  PUBMED    31069270
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-05-3"; volume: "2:161. doi"; pages: " 
            10.1038/s42003-019-0402-x. eCollection 2019"
FEATURES             Location/Qualifiers
     source          1..8647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        75..103
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    109..125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     5'UTR           147..280
                     /gene="cspA"
                     /label=cspA 5'UTR
                     /note="5'UTR of the E. coli cold shock protein cspA gene 
                     (Mitta et al., 1997)"
     CDS             281..292
                     /codon_start=1
                     /label=start
                     /translation="MKSS"
     CDS             293..310
                     /codon_start=1
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             317..721
                     /codon_start=1
                     /label=CL7
                     /translation="SKSNEPGKATGEGKPVNNKWLNNAGKDLGSPVPDRIANKLRDKEF
                     ESFDDFRETFWEEVSKDPELSKQFSRNNNDRMKVGKAPKTRTQDVSGKRTSFELNHQKP
                     IEQNGGVYDMDNISVVTPKRNIDIEGGGGGS"
     CDS             722..745
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             755..775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             785..805
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             833..4936
                     /codon_start=1
                     /label=Cas9
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRISYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIESFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    860..862
                     /label=D10 locus
     misc_feature    1070..1072
                     /label=C80S
     misc_feature    2552..2554
                     /label=C574S
     misc_feature    3350..3352
                     /label=H840 Locus
     CDS             4943..5008
                     /codon_start=1
                     /label=A22p
                     /translation="HTPGNSNKWKHLQENKKGRPRR"
     CDS             5018..5083
                     /codon_start=1
                     /label=A22p
                     /translation="HTPGNSNKWKHLQENKKGRPRR"
     CDS             5093..5158
                     /codon_start=1
                     /label=A22p
                     /translation="HTPGNSNKWKHLQENKKGRPRR"
     3'UTR           5169..5313
                     /gene="cspA"
                     /label=cspA 3'UTR
                     /note="3'UTR of the E. coli cold shock protein cspA gene 
                     (Mitta et al., 1997)"
     terminator      5389..5460
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        5566..5670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5671..6531
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6702..7290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7462..8544)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(8545..8622)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
       61 aaggaatggt gtggttgaca attaatcatc ggctcgtata atgtgtaatt gtgagcggat
      121 aacaatttga tgtgctagcg catatccagt gtagtaaggc aagtcccttc aagagttatc
      181 gttgataccc ctcgtagtgc acattccttt aacgcttcaa aatctgtaaa gcacgccata
      241 tcgccgaaag gcacacttaa ttattaagag gtaatacacc atgaaatctt ctcaccatca
      301 ccatcaccat ggttctagca aaagcaatga accgggtaaa gcaaccggtg aaggtaaacc
      361 ggttaataac aaatggctga acaatgccgg taaagatctg ggtagtccgg ttccggatcg
      421 tattgcaaat aaactgcgtg ataaagaatt cgagagcttc gatgattttc gtgaaacctt
      481 ttgggaagaa gttagcaaag atcctgaact gagcaaacag tttagccgca ataacaatga
      541 tcgtatgaaa gttggtaaag caccgaaaac acgtacccag gatgttagcg gtaaacgtac
      601 ctcatttgaa ctgaatcatc agaaaccgat tgaacagaat ggtggcgttt atgatatgga
      661 taacattagc gttgttaccc cgaaacgcaa cattgatatt gaaggcggtg gtggtggttc
      721 tttggaggtt ttgttccagg gtccaaacgc gacaccaaag aaaaagcgta aagtcggggg
      781 atctccgaaa aagaaacgga aagtaggtat ccacggagtc ccagcagcta ccatggataa
      841 gaaatactca ataggcttag atatcggcac aaatagcgtc ggatgggcgg tgatcactga
      901 tgaatataag gttccgtcta aaaagttcaa ggttctggga aatacagacc gccacagtat
      961 caaaaaaaat cttatagggg ctcttttatt tgacagtgga gagacagcgg aagcgactcg
     1021 tctcaaacgg acagctcgta gaaggtatac acgtcggaag aatcgtatta gctatctaca
     1081 ggagattttt tcaaatgaga tggcgaaagt agatgatagt ttctttcatc gacttgaaga
     1141 gtcttttttg gtggaagaag acaagaagca tgaacgtcat cctatttttg gaaatatagt
     1201 agatgaagtt gcttatcatg agaaatatcc aactatctat catctgcgaa aaaaattggt
     1261 agattctact gataaagcgg atttgcgctt aatctatttg gccttagcgc atatgattaa
     1321 gtttcgtggt cattttttga ttgagggaga tttaaatcct gataatagtg atgtggacaa
     1381 actatttatc cagttggtac aaacctacaa tcaattattt gaagaaaacc ctattaacgc
     1441 aagtggagta gatgctaaag cgattctttc tgcacgattg agtaaatcaa gacgattaga
     1501 aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc ttatttggga atctcattgc
     1561 tttgtcattg ggtttgaccc ctaattttaa atcaaatttt gatttggcag aagatgctaa
     1621 attacagctt tcaaaagata cttacgatga tgatttagat aatttattgg cgcaaattgg
     1681 agatcaatat gctgatttgt ttttggcagc taagaattta tcagatgcta ttttactttc
     1741 agatatccta agagtaaata ctgaaataac taaggctccc ctatcagctt caatgattaa
     1801 acgctacgat gaacatcatc aagacttgac tcttttaaaa gctttagttc gacaacaact
     1861 tccagaaaag tataaagaaa tcttttttga tcaatcaaaa aacggatatg caggttatat
     1921 tgatggggga gctagccaag aagaatttta taaatttatc aaaccaattt tagaaaaaat
     1981 ggatggtact gaggaattat tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg
     2041 gacctttgac aacggctcta ttccccatca aattcacttg ggtgagctgc atgctatttt
     2101 gagaagacaa gaagactttt atccattttt aaaagacaat cgtgagaaga ttgaaaaaat
     2161 cttgactttt cgaattcctt attatgttgg tccattggcg cgtggcaata gtcgttttgc
     2221 atggatgact cggaagtctg aagaaacaat taccccatgg aattttgaag aagttgtcga
     2281 taaaggtgct tcagctcaat catttattga acgcatgaca aactttgata aaaatcttcc
     2341 aaatgaaaaa gtactaccaa aacatagttt gctttatgag tattttacgg tttataacga
     2401 attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga
     2461 acagaagaaa gccattgttg atttactctt caaaacaaat cgaaaagtaa ccgttaagca
     2521 attaaaagaa gattatttca aaaaaataga aagctttgat agtgttgaaa tttcaggagt
     2581 tgaagataga tttaatgctt cattaggtac ctaccatgat ttgctaaaaa ttattaaaga
     2641 taaagatttt ttggataatg aagaaaatga agatatctta gaggatattg ttttaacatt
     2701 gaccttattt gaagataggg agatgattga ggaaagactt aaaacatatg ctcacctctt
     2761 tgatgataag gtgatgaaac agcttaaacg tcgccgttat actggttggg gacgtttgtc
     2821 tcgaaaattg attaatggta ttagggataa gcaatctggc aaaacaatat tagatttttt
     2881 gaaatcagat ggttttgcca atcgcaattt tatgcagctg atccatgatg atagtttgac
     2941 atttaaagaa gacattcaaa aagcacaagt gtctggacaa ggcgatagtt tacatgaaca
     3001 tattgcaaat ttagctggta gccctgctat taaaaaaggt attttacaga ctgtaaaagt
     3061 tgttgatgaa ttggtcaaag taatggggcg gcataagcca gaaaatatcg ttattgaaat
     3121 ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg
     3181 aatcgaagaa ggtatcaaag aattaggaag tcagattctt aaagagcatc ctgttgaaaa
     3241 tactcaattg caaaatgaaa agctctatct ctattatctc caaaatggaa gagacatgta
     3301 tgtggaccaa gaattagata ttaatcgttt aagtgattat gatgtcgatc acattgttcc
     3361 acaaagtttc cttaaagacg attcaataga caataaggtc ttaacgcgtt ctgataaaaa
     3421 tcgtggtaaa tcggataacg ttccaagtga agaagtagtc aaaaagatga aaaactattg
     3481 gagacaactt ctaaacgcca agttaatcac tcaacgtaag tttgataatt taacgaaagc
     3541 tgaacgtgga ggtttgagtg aacttgataa agctggtttt atcaaacgcc aattggttga
     3601 aactcgccaa atcactaagc atgtggcaca aattttggat agtcgcatga atactaaata
     3661 cgatgaaaat gataaactta ttcgagaggt taaagtgatt accttaaaat ctaaattagt
     3721 ttctgacttc cgaaaagatt tccaattcta taaagtacgt gagattaaca attaccatca
     3781 tgcccatgat gcgtatctaa atgccgtcgt tggaactgct ttgattaaga aatatccaaa
     3841 acttgaatcg gagtttgtct atggtgatta taaagtttat gatgttcgta aaatgattgc
     3901 taagtctgag caagaaatag gcaaagcaac cgcaaaatat ttcttttact ctaatatcat
     3961 gaacttcttc aaaacagaaa ttacacttgc aaatggagag attcgcaaac gccctctaat
     4021 cgaaactaat ggggaaactg gagaaattgt ctgggataaa gggcgagatt ttgccacagt
     4081 gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag tacagacagg
     4141 cggattctcc aaggagtcaa ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa
     4201 aaaagactgg gatccaaaaa aatatggtgg ttttgatagt ccaacggtag cttattcagt
     4261 cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt
     4321 actagggatc acaattatgg aaagaagttc ctttgaaaaa aatccgattg actttttaga
     4381 agctaaagga tataaggaag ttaaaaaaga cttaatcatt aaactaccta aatatagtct
     4441 ttttgagtta gaaaacggtc gtaaacggat gctggctagt gccggagaat tacaaaaagg
     4501 aaatgagctg gctctgccaa gcaaatatgt gaatttttta tatttagcta gtcattatga
     4561 aaagttgaag ggtagtccag aagataacga acaaaaacaa ttgtttgtgg agcagcataa
     4621 gcattattta gatgagatta ttgagcaaat cagtgaattt tctaagcgtg ttattttagc
     4681 agatgccaat ttagataaag ttcttagtgc atataacaaa catagagaca aaccaatacg
     4741 tgaacaagca gaaaatatta ttcatttatt tacgttgacg aatcttggag ctcccgctgc
     4801 ttttaaatat tttgatacaa caattgatcg taaacgatat acgtctacaa aagaagtttt
     4861 agatgccact cttatccatc aatccatcac tggtctttat gaaacacgca ttgatttgag
     4921 tcagctagga ggtgacgggt cacatacacc cgggaactcg aataaatgga aacacttaca
     4981 agagaataaa aagggccgtc cgcgtagagg tggtagtcac actccaggaa attccaacaa
     5041 atggaagcac ttgcaggaga acaaaaaggg tcgtccacgg cgtggtggct cccatacgcc
     5101 aggaaacagt aataagtgga aacatttgca agaaaacaaa aaagggcgtc cgcgtcggta
     5161 ataggtaatc tctgcttaaa agcacagaat ctaagatccc tgccatttgg cggggatttt
     5221 tttatttgtt ttcaggaaat aaataatcga tcgcgtaata aaatctatta ttatttttgt
     5281 gaagaataaa tttgggtgca atgagaatgc gcaggccctt tcgtctcgcg cgtttcggtg
     5341 atgacggtga aaacctctga cacatgtcga gtaaggatct ccaggcatca aataaaacga
     5401 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     5461 gtcgacacta tggttgcttt gacgtatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5521 aaaataccgc atcaggcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5581 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5641 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5701 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5761 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5821 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5881 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5941 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6001 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6061 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6121 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6181 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6241 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6301 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6361 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6421 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6481 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6541 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6601 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6661 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6721 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6781 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6841 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6901 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6961 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7021 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7081 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7141 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7201 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7261 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7321 ctggcctttt gctggccttt tgctcacata gtcatgcccc gcgcccaccg gaaggagctg
     7381 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
     7441 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7501 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     7561 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     7621 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     7681 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     7741 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     7801 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     7861 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     7921 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     7981 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     8041 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     8101 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     8161 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     8221 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     8281 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     8341 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     8401 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     8461 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     8521 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
     8581 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     8641 ccttatg
//
