LOCUS       223016-attachmen       22605 bp    DNA     circular SYN 13-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 13, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..22605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(48..308)
                     /label=bom site from pBR322
                     /note="bom site from pBR322"
     rep_origin      complement(448..728)
                     /direction=LEFT
                     /label=pBR322 origin of replication
                     /note="pBR322 origin of replication"
     CDS             1155..1964
                     /label=Kana Resistance
     misc_feature    2220..2257
                     /label=Left border
                     /label=nonstandard type: Region
     misc_feature    2280..3824
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pMB19 (8.202bp circular)Fragment: from BpiI 
                     cut
                     at 1596^1597 to BpiI cut at 3141^3142,
                     1.545bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    2284..2594
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI A-B NOSpro (G80) (2,791bp 
                     circular)Fragment:
                     from BsaI cut at 963^964 to BsaI cut at 1274^1275, 
                     311bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     promoter        2288..2594
                     /label=NOSpro
     misc_feature    2595..2602
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI dy B-C - BB6 (2,487bp circular)Fragment: 
                     from
                     BsaI cut at 1590^1591 to BsaI cut at 1598^1599, 
                     8bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    2599..2602
                     /label=DMY
                     /note="/feature_type=Misc."
     misc_feature    2603..3542
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=Renilla luciferase L1 (4,396bp
                     circular)Fragment: from BsaI cut at 54^55 to BsaI cut at 
                     994^995, 940bpOrientation: originalPlus strand 5':   no 
                     changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     CDS             2607..3542
                     /label=Renilla luciferase
     misc_feature    3543..3568
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI dy D-E - BB8 (2,478bp circular)Fragment: 
                     from
                     BsaI cut at 827^828 to BsaI cut at 853^854, 
                     26bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    3547..3568
                     /label=DMY
                     /note="/feature_type=Misc."
     misc_feature    3569..3798
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI E-F 35Sterm (G59) (2,734bp 
                     circular)Fragment:
                     from BsaI cut at 1933^1934 to BsaI cut at 2163^2164, 
                     230bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     terminator      3573..3798
                     /label=35Sterm
     misc_feature    3799..3820
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI dy F-G - BB9 (2,500bp circular)Fragment: 
                     from
                     BsaI cut at 1212^1213 to BsaI cut at 1234^1235, 
                     22bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    3803..3820
                     /label=DMY
                     /note="/feature_type=Misc."
     misc_feature    join(3821..3824,2280..2283)
                     /label=nonstandard type: Vector
                     /note="/origin=LII? F 1-2 - BB20 (8,260bp circular)Target
                     vector: from BsaI cut at 3195^3196 to BsaI cut at 
                     1600^1601, 6,665bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    3825..10427
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pMB99 (13.260bp circular)Fragment: from BpiI
                     cut
                     at 1596^1597 to BpiI cut at 8199^8200,
                     6.603bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    3829..4667
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI A-B 35Spro (G5) (3.319bp 
                     circular)Fragment:
                     from BsaI cut at 892^893 to BsaI cut at 1731^1732, 
                     839bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     promoter        3833..4667
                     /label=35Spro
     misc_feature    4668..4731
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI B-C N-NLS  (G60) (2.544bp 
                     circular)Fragment:
                     from BsaI cut at 1196^1197 to BsaI cut at 1260^1261, 
                     64bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    4672..4675
                     /label=Plant Kozak
                     /label=nonstandard type: Region
     misc_feature    4676..4712
                     /label=SV40
                     /label=nonstandard type: NLS
                     /note="NLS SV40 T-antigen"
     misc_feature    4732..6125
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=At_Cas9 (fragment 1); L1 (6.625bp
                     circular)Fragment: from BsaI cut at 965^966 to BsaI cut
                     at 5106^5107, 4.141bpOrientation: originalPlus strand 5': 
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    4736..8851
                     /label=At_Cas9
                     /note="/feature_type=At_Cas9"
     misc_feature    4736..6125
                     /label=At_Cas9 (part 1)
                     /note="/feature_type=At_Cas9 (parts)"
     misc_feature    6126..7490
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=At_Cas9 (fragment 2); L1 (3.849bp
                     circular)Fragment: from BsaI cut at 965^966 to BsaI cut
                     at 2330^2331, 1.365bpOrientation: originalPlus strand 5': 
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    6126..7490
                     /label=At_Cas9 (part 2)
                     /note="/feature_type=At_Cas9 (parts)"
     misc_feature    7491..8851
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=At_Cas9 (fragment 3); L1 (3.845bp
                     circular)Fragment: from BsaI cut at 965^966 to BsaI cut
                     at 2326^2327, 1.361bpOrientation: originalPlus strand 5': 
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    7491..8851
                     /label=At_Cas9 (part 3)
                     /note="/feature_type=At_Cas9 (parts)"
     misc_feature    8852..8908
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI D-E C-NLS (G35) (2.537bp 
                     circular)Fragment:
                     from BsaI cut at 1058^1059 to BsaI cut at 1115^1116, 
                     57bpOrientation: reverse complementedPlus strand 5':   no 
                     changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    8858..8891
                     /label=SV40
                     /label=nonstandard type: NLS
     misc_feature    8909..9950
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=NbACT3term; L1 (4.498bp circular)Fragment: 
                     from
                     BsaI cut at 54^55 to BsaI cut at 1096^1097, 
                     1.042bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     terminator      8913..9950
                     /label=NbACT3term
     primer_bind     complement(9178..9202)
                     /label=DC-1763 NbACT3 Term E-F R1
     primer_bind     9205..9225
                     /label=DC-1764 NbACT3 Term E-F F2
     misc_feature    9951..10423
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=PsRBCS-3Aterm; L1 (3.929bp 
                     circular)Fragment:
                     from BsaI cut at 54^55 to BsaI cut at 527^528, 
                     473bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     terminator      9955..10423
                     /label=PsRBCS-3Aterm
     misc_feature    join(10424..10427,3825..3828)
                     /label=nonstandard type: Vector
                     /note="/origin=LII? F 2-3 - BB22 (8.260bp circular)Target
                     vector: from BsaI cut at 3195^3196 to BsaI cut at 
                     1600^1601, 6.665bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    10428..13005
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pMB42 (9.235bp circular)Fragment: from BpiI 
                     cut
                     at 1596^1597 to BpiI cut at 4174^4175,
                     2.578bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    10432..11232
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LjU6-1pro-Spc1 (I); L1  (3.285bp
                     circular)Fragment: from BsaI cut at 965^966 to BsaI cut
                     at 1766^1767, 801bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     promoter        10436..11235
                     /label=LjU6-1pro
     variation       11026
                     /label=G ? C
     variation       11091
                     /label=T ? A
     variation       11213
                     /label=G ? C
                     /note="L.japonicus MG20: G; L.japonicus Gifu: C"
     misc_feature    11233..11260
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=Oligonucleotide_ Spacer 1 (I) for 2x LjU6pro
                     (Cas9); L1  (2.512bp circular)Fragment: from BsaI cut at 
                     965^966 to BsaI cut at 993^994, 28bpOrientation: 
                     originalPlus strand 5':   no changePlus strand 3':   no 
                     changeMinus strand 5':  no changeMinus strand 3':  no 
                     change"
     misc_feature    11236
                     /label=Additional G
     misc_feature    11237..11256
                     /label=Spacer-1 (I)
                     /label=nonstandard type: Spacer
     misc_feature    11257..11332
                     /label=nonstandard type: tracr
     misc_feature    11261..11716
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=tracr-U6term; L1  (2.940bp 
                     circular)Fragment:
                     from BsaI cut at 965^966 to BsaI cut at 1421^1422, 
                     456bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     terminator      11333..11705
                     /label=U6-1term
     misc_feature    11333..11339
                     /label=Poly-T
     misc_feature    11717..12524
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LjU6-1pro-Spc2 (I); L1  (3.292bp
                     circular)Fragment: from BsaI cut at 965^966 to BsaI cut
                     at 1773^1774, 808bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     promoter        11732..12531
                     /label=LjU6-1pro
     variation       12322
                     /label=G ? C
     variation       12387
                     /label=T ? A
     variation       12509
                     /label=G ? C
                     /note="L.japonicus MG20: G; L.japonicus Gifu: C"
     misc_feature    12525..12552
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=Oligonucleotide_ Spacer 2 (I) for 2x LjU6pro
                     (Cas9); L1 (2.512bp circular)Fragment: from BsaI cut at 
                     965^966 to BsaI cut at 993^994, 28bpOrientation: 
                     originalPlus strand 5':   no changePlus strand 3':   no 
                     changeMinus strand 5':  no changeMinus strand 3':  no 
                     change"
     misc_feature    12532
                     /label=Additional G
     misc_feature    12533..12552
                     /label=Spacer-2 (I)
                     /label=nonstandard type: Spacer
     misc_feature    12553..13001
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=tracr-U6term; L1 (2.933bp circular)Fragment:
                     from BsaI cut at 965^966 to BsaI cut at 1414^1415, 
                     449bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    12553..12628
                     /label=nonstandard type: tracr
     terminator      12629..13001
                     /label=U6-1term
     misc_feature    12629..12635
                     /label=Poly-T
     misc_feature    join(13002..13005,10428..10431)
                     /label=nonstandard type: Vector
                     /note="/origin=LII? F 3-4 - BB24 (8.260bp circular)Target
                     vector: from BsaI cut at 3195^3196 to BsaI cut at 
                     1600^1601, 6.665bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    13006..16180
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pMB14 (9.832bp circular)Fragment: from BpiI 
                     cut
                     at 1596^1597 to BpiI cut at 4771^4772,
                     3.175bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    13010..14135
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI A-B Ubi (G7) (3,605bp circular)Fragment: 
                     from
                     BsaI cut at 1770^1771 to BsaI cut at 2896^2897, 
                     1,126bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     promoter        13014..13548
                     /note="LjUbi10pro"
     gene            13549..14135
                     /gene="LjUbi10pro"
                     /label=LjUbi10pro
     misc_feature    14136..14217
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=ATG:HP (Firefly Luciferase)  (8,950bp
                     circular)Fragment: from BpiI cut at 1596^1597 to BpiI cut 
                     at 3889^3890, 2,293bpOrientation: originalPlus strand 5': 
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     CDS             14144..15871
                     /label=ATG:6xHis:HP:Firefly luciferase
     CDS             14144..14146
                     /label=ATG (Firefly luciferase)
     misc_feature    14153..14170
                     /label=6xHis-Tag
     misc_feature    14181..14208
                     /label=28-nt repeat with hairpin (HP)
     misc_feature    14218..15871
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=Firefly luciferase (4,138bp 
                     circular)Fragment:
                     from BsaI cut at 965^966 to BsaI cut at 2619^2620, 
                     1,654bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     CDS             14219..15871
                     /label=Firefly luciferase without start codon
     misc_feature    15872..15897
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI dy D-E - BB8 (2,478bp circular)Fragment: 
                     from
                     BsaI cut at 827^828 to BsaI cut at 853^854, 
                     26bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    15876..15897
                     /label=DMY
                     /note="/feature_type=Misc."
     misc_feature    15898..16154
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI E-F NOSterm (G6) (2,737bp 
                     circular)Fragment:
                     from BsaI cut at 942^943 to BsaI cut at 1199^1200, 
                     257bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     terminator      15902..16154
                     /label=NOSterm
     misc_feature    16155..16176
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI dy F-G - BB9 (2,500bp circular)Fragment: 
                     from
                     BsaI cut at 1212^1213 to BsaI cut at 1234^1235, 
                     22bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    16159..16176
                     /label=DMY
                     /note="/feature_type=Misc."
     misc_feature    join(16177..16180,13006..13009)
                     /label=nonstandard type: Vector
                     /note="/origin=LII? F 4-5 - BB26 (8,260bp circular)Target
                     vector: from BsaI cut at 3195^3196 to BsaI cut at 
                     1600^1601, 6,665bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    16181..18525
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pMB15 (9.002bp circular)Fragment: from BpiI 
                     cut
                     at 1596^1597 to BpiI cut at 3941^3942,
                     2.345bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    16185..17615
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=Act2pro L1 (3.916bp circular)Fragment: from 
                     BsaI
                     cut at 965^966 to BsaI cut at 2396^2397, 
                     1.431bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     promoter        16189..17615
                     /label=Act2pro
                     /note="/origin=AtAct2pro A-B (1,478bp linear)Fragment: from
                     BpiI cut at 10^11 to BpiI cut at 1464^1465, 
                     1,454bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     primer_bind     complement(17049..17075)
                     /label=R1 pUC Bpi
     primer_bind     17072..17098
                     /label=F2 pUC Bpi
     primer_bind     17072
                     /label=A to T pUC Bpi
     misc_feature    17616..17646
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=5e target (I) (77bp linear)Fragment: from 
                     BsaI
                     cut at 21^22 to BsaI cut at 52^53, 31bpOrientation: 
                     originalPlus strand 5':   no changePlus strand 3':   no 
                     changeMinus strand 5':  no changeMinus strand 3':  no 
                     change"
     misc_feature    complement(17620..17622)
                     /direction=LEFT
                     /label=PAM (Cas9)
                     /label=nonstandard type: PAM
     misc_feature    complement(17623..17642)
                     /direction=LEFT
                     /label=5' target (I)
                     /label=nonstandard type: target
     misc_feature    complement(17643..17646)
                     /direction=LEFT
                     /label=PAM (Cas12a)
                     /label=nonstandard type: PAM
     misc_feature    17647..18214
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=Csy4 L1 (3.052bp circular)Fragment: from 
                     BsaI
                     cut at 965^966 to BsaI cut at 1533^1534,
                     568bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     CDS             17651..18214
                     /label=Csy4
     misc_feature    18215..18245
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=3' target (I) (77bp linear)Fragment: from 
                     BsaI
                     cut at 21^22 to BsaI cut at 52^53, 31bpOrientation: 
                     originalPlus strand 5':   no changePlus strand 3':   no 
                     changeMinus strand 5':  no changeMinus strand 3':  no 
                     change"
     misc_feature    18219..18222
                     /label=PAM (Cas12a)
                     /label=nonstandard type: PAM
     misc_feature    18223..18242
                     /label=3' target (I)
                     /label=nonstandard type: target
     misc_feature    18243..18245
                     /label=PAM (Cas9)
                     /label=nonstandard type: PAM
     misc_feature    18246..18499
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI E-F HSPterm (G45)-1 (2.733bp
                     circular)Fragment: from BsaI cut at 588^589 to BsaI cut
                     at 842^843, 254bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     terminator      18250..18499
                     /label=HSPterm
     misc_feature    18500..18521
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=LI dy F-G - BB9 (2.500bp circular)Fragment: 
                     from
                     BsaI cut at 1212^1213 to BsaI cut at 1234^1235, 
                     22bpOrientation: originalPlus strand 5':   no changePlus 
                     strand 3':   no changeMinus strand 5':  no changeMinus 
                     strand 3':  no change"
     misc_feature    18504..18521
                     /label=DMY
                     /note="/feature_type=Misc."
     misc_feature    join(18522..18525,16181..16184)
                     /label=nonstandard type: Vector
                     /note="/origin=LII? F 5-6 - BB28-1 (8.260bp circular)Target
                     vector: from BsaI cut at 3195^3196 to BsaI cut at 
                     1600^1601, 6.665bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    join(18526..22605,1..2279)
                     /label=nonstandard type: Vector
                     /note="/origin=LIII? F A-B - BB53 (7.956bp circular)Target
                     vector: from BpiI cut at 3876^3877 to BpiI cut at 
                     2279^2280, 6.359bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no 
                     changeMinus strand 3':  no change"
     misc_feature    complement(18563..18607)
                     /label=Right border
                     /label=nonstandard type: Region
     CDS             18903..19589
                     /label=pVS1 Resolvase
     rep_origin      complement(19888..22279)
                     /direction=LEFT
                     /label=pVS1 ORI
ORIGIN
        1 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
       61 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
      121 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
      181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      781 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      961 ctgacgctca taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     1021 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     1081 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     1141 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     1201 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1261 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1321 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1381 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1441 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1501 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     1561 attgtccttt taacagcgat cgcgtatttc gtcttgctca ggcgcaatca cgaatgaata
     1621 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     1681 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     1741 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     1801 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     1861 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     1921 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     1981 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     2041 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agacccctgc cggtaacatg
     2101 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
     2161 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     2221 ggcaggatat attgtggtgt aaacaaattg acgcttagac ggattacggg tctcagcggt
     2281 acggcgggat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
     2341 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
     2401 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     2461 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
     2521 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
     2581 tctgcaccgg atcttctgga agcaccatga cgagtaaagt gtatgatcct gagcaaagga
     2641 agagaatgat cacaggtcct caatggtggg ctaggtgtaa acagatgaat gttcttgact
     2701 ctttcatcaa ctactacgat tctgagaaac atgccgaaaa cgcagtgatt ttcctccatg
     2761 gaaatgctgc ttcaagctat ctctggagac acgttgtccc acatatagaa ccagtagcta
     2821 gatgcattat accggatttg attggtatgg gcaaaagtgg gaaatcaggg aatggatcct
     2881 acaggttatt ggaccactac aagtatctca cagcttggtt tgaactactt aacttgccta
     2941 agaagatcat attcgtggga catgattggg gagcatgtct tgcctttcac tattcctatg
     3001 aacatcagga caaaataaag gccattgtcc atgcagagtc tgttgttgac gtgattgaaa
     3061 gctgggatga atggcctgac attgaggagg atattgcgtt aatcaagagt gaagaaggcg
     3121 agaaaatggt tcttgaaaac aatttctttg tcgaaactat gttgccgtct aaaatcatga
     3181 gaaaactgga accagaagaa ttcgcagcat acttagaacc ctttaaggag aaaggagaag
     3241 tcagacgacc aactctgtct tggcctagag agattccatt ggttaagggt ggcaaaccag
     3301 atgttgtaca aattgtgcga aattataacg cttatctgcg tgcttcagat gatcttccta
     3361 agatgtttat tgagtcagat cccggtttct ttagcaatgc gattgtagag ggtgctaaga
     3421 aatttccaaa taccgaattc gtgaaggtta aaggactaca cttttctcaa gaagatgcac
     3481 ctgatgagat ggggaagtac ataaagtcct ttgtagagcg tgttcttaag aatgagcagt
     3541 aaaagggcta agacctaagc tagcctagaa tccggccatg ctagagtccg caaaaatcac
     3601 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt
     3661 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     3721 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     3781 accaaaatcc agtgaccttg agtgacctaa gctagcctag tgtcgggcgc ggagattagc
     3841 cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta cgcagcacga
     3901 ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct tcccaagaag
     3961 gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata
     4021 tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct tcacaaacca
     4081 aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc aaaggccatg
     4141 gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc
     4201 atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac
     4261 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagagca aagggcaatt
     4321 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc
     4381 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc
     4441 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
     4501 ccacccacga ggagcatcgt ggaaaaagaa cacgttccaa ccacgacttc aaagcaagtg
     4561 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     4621 gacccttcct ctatataagg aagttcattt catttggaga gaacacgtct gaacaatgct
     4681 gcagcctaag aagaagagaa aggttggagg agtcgactcg agtgcggccg ccaccatgga
     4741 taagaagtac tctatcggac tcgatatcgg aactaactct gtgggatggg ctgtgatcac
     4801 cgatgagtac aaggtgccat ctaagaagtt caaggttctc ggaaacaccg ataggcactc
     4861 tatcaagaaa aaccttatcg gtgctctcct cttcgattct ggtgaaactg ctgaggctac
     4921 cagactcaag agaaccgcta gaagaaggta caccagaaga aagaacagga tctgctacct
     4981 ccaagagatc ttctctaacg agatggctaa agtggatgat tcattcttcc acaggctcga
     5041 agagtcattc ctcgtggaag aagataagaa gcacgagagg caccctatct tcggaaacat
     5101 cgttgatgag gtggcatacc acgagaagta ccctactatc taccacctca gaaagaagct
     5161 cgttgattct actgataagg ctgatctcag gctcatctac ctcgctctcg ctcacatgat
     5221 caagttcaga ggacacttcc tcatcgaggg tgatctcaac cctgataact ctgatgtgga
     5281 taagttgttc atccagctcg tgcagaccta caaccagctt ttcgaagaga accctatcaa
     5341 cgcttcaggt gtggatgcta aggctatcct ctctgctagg ctctctaagt caagaaggct
     5401 tgagaacctc attgctcagc tccctggtga gaagaagaac ggacttttcg gaaacttgat
     5461 cgctctctct ctcggactca cccctaactt caagtctaac ttcgatctcg ctgaggatgc
     5521 aaagctccag ctctcaaagg atacctacga tgatgatctc gataacctcc tcgctcagat
     5581 cggagatcag tacgctgatt tgttcctcgc tgctaagaac ctctctgatg ctatcctcct
     5641 cagtgatatc ctcagagtga acaccgagat caccaaggct ccactctcag cttctatgat
     5701 caagagatac gatgagcacc accaggatct cacacttctc aaggctcttg ttagacagca
     5761 gctcccagag aagtacaaag agattttctt cgatcagtct aagaacggat acgctggtta
     5821 catcgatggt ggtgcatctc aagaagagtt ctacaagttc atcaagccta tcctcgagaa
     5881 gatggatgga accgaggaac tcctcgtgaa gctcaataga gaggatcttc tcagaaagca
     5941 gaggaccttc gataacggat ctatccctca tcagatccac ctcggagagt tgcacgctat
     6001 ccttagaagg caagaggatt tctacccatt cctcaaggat aacagggaaa agattgagaa
     6061 gattctcacc ttcagaatcc cttactacgt gggacctctc gctagaggaa actcaagatt
     6121 cgcttggatg accagaaagt ctgaggaaac catcacccct tggaacttcg aagaggtggt
     6181 ggataagggt gctagtgctc agtctttcat cgagaggatg accaacttcg ataagaacct
     6241 tccaaacgag aaggtgctcc ctaagcactc tttgctctac gagtacttca ccgtgtacaa
     6301 cgagttgacc aaggttaagt acgtgaccga gggaatgagg aagcctgctt ttttgtcagg
     6361 tgagcaaaag aaggctatcg ttgatctctt gttcaagacc aacagaaagg tgaccgtgaa
     6421 gcagctcaaa gaggattact tcaagaaaat cgagtgcttc gattcagttg agatttctgg
     6481 tgttgaggat aggttcaacg catctctcgg aacctaccac gatctcctca agatcattaa
     6541 ggataaggat ttcttggata acgaggaaaa cgaggatatc ttggaggata tcgttcttac
     6601 cctcaccctc tttgaagata gagagatgat tgaagaaagg ctcaagacct acgctcatct
     6661 cttcgatgat aaggtgatga agcagttgaa gagaagaaga tacactggtt ggggaaggct
     6721 ctcaagaaag ctcattaacg gaatcaggga taagcagtct ggaaagacaa tccttgattt
     6781 cctcaagtct gatggattcg ctaacagaaa cttcatgcag ctcatccacg atgattctct
     6841 cacctttaaa gaggatatcc agaaggctca ggtttcagga cagggtgata gtctccatga
     6901 gcatatcgct aacctcgctg gatctcctgc aatcaagaag ggaatcctcc agactgtgaa
     6961 ggttgtggat gagttggtga aggtgatggg aaggcataag cctgagaaca tcgtgatcga
     7021 aatggctaga gagaaccaga ccactcagaa gggacagaag aactctaggg aaaggatgaa
     7081 gaggatcgag gaaggtatca aagagcttgg atctcagatc ctcaaagagc accctgttga
     7141 gaacactcag ctccagaatg agaagctcta cctctactac ctccagaacg gaagggatat
     7201 gtatgtggat caagagttgg atatcaacag gctctctgat tacgatgttg atcatatcgt
     7261 gccacagtca ttcttgaagg atgattctat cgataacaag gtgctcacca ggtctgataa
     7321 gaacaggggt aagagtgata acgtgccaag tgaagaggtt gtgaagaaaa tgaagaacta
     7381 ttggaggcag ctcctcaacg ctaagctcat cactcagaga aagttcgata acttgactaa
     7441 ggctgagagg ggaggactct ctgaattgga taaggcagga ttcatcaaga ggcagcttgt
     7501 ggaaaccagg cagatcacta agcacgttgc acagatcctc gattctagga tgaacaccaa
     7561 gtacgatgag aacgataagt tgatcaggga agtgaaggtt atcaccctca agtcaaagct
     7621 cgtgtctgat ttcagaaagg atttccaatt ctacaaggtg agggaaatca acaactacca
     7681 ccacgctcac gatgcttacc ttaacgctgt tgttggaacc gctctcatca agaagtatcc
     7741 taagctcgag tcagagttcg tgtacggtga ttacaaggtg tacgatgtga ggaagatgat
     7801 cgctaagtct gagcaagaga tcggaaaggc taccgctaag tatttcttct actctaacat
     7861 catgaatttc ttcaagaccg agattaccct cgctaacggt gagatcagaa agaggccact
     7921 catcgagaca aacggtgaaa caggtgagat cgtgtgggat aagggaaggg atttcgctac
     7981 cgttagaaag gtgctctcta tgccacaggt gaacatcgtt aagaaaaccg aggtgcagac
     8041 cggtggattc tctaaagagt ctatcctccc taagaggaac tctgataagc tcattgctag
     8101 gaagaaggat tgggacccta agaaatacgg tggtttcgat tctcctaccg tggcttactc
     8161 tgttctcgtt gtggctaagg ttgagaaggg aaagagtaag aagctcaagt ctgttaagga
     8221 acttctcgga atcactatca tggaaaggtc atctttcgag aagaacccaa tcgatttcct
     8281 cgaggctaag ggatacaaag aggttaagaa ggatctcatc atcaagctcc caaagtactc
     8341 actcttcgaa ctcgagaacg gtagaaagag gatgctcgct tctgctggtg agcttcaaaa
     8401 gggaaacgag cttgctctcc catctaagta cgttaacttt ctttacctcg cttctcacta
     8461 cgagaagttg aagggatctc cagaagataa cgagcagaag caacttttcg ttgagcagca
     8521 caagcactac ttggatgaga tcatcgagca gatctctgag ttctctaaaa gggtgatcct
     8581 cgctgatgca aacctcgata aggtgttgtc tgcttacaac aagcacagag ataagcctat
     8641 cagggaacag gcagagaaca tcatccatct cttcaccctt accaacctcg gtgctcctgc
     8701 tgctttcaag tacttcgata caaccatcga taggaagaga tacacctcta ccaaagaagt
     8761 gctcgatgct accctcatcc atcagtctat cactggactc tacgagacta ggatcgatct
     8821 ctcacagctc ggtggtgatt caagggctga taagggactg cagcctaaga agaagagaaa
     8881 ggttggagga gtcgactcga gtgcgtaaaa tcatacagca ttcccagaaa gagaaacaga
     8941 agaaatatac aaactttcat tttgagagca gcacctcgtc tattgattgc agataatatg
     9001 cttctcattt gtatttcctt ttgattattt ttgtttctat ccctttgttt gagtcaatct
     9061 caaatattcg gtcattgttg gtatgaaaaa tcaagcagtt catgttaaga gtcaatttaa
     9121 aattaatatt tttatataga gttgtatgtg aaatgatgtt gtgatttggt atatatggat
     9181 aaagagcttg tcagttcatt ttgctctcat ttttttggta tccaaataag aaacacaaaa
     9241 gggatatgtc cctctactat caaatattag ttataagtat tcatgttata ctattcgata
     9301 ttttctaccc caatcgttac ctatttaaaa gtatttaccc ctccatctat caaacccctg
     9361 gacccagctt tcctattaca tgtggcttca tcttaagccc ccaaaccttt ttcttatttt
     9421 tgatttttaa aggctcatct taaaatttat tactcaaatt aatacctctt aataacccac
     9481 ctcaaggacc cagtaattaa atatccaatt agctccagta attggggttc atattagctc
     9541 cagtcttaaa ttttaaaggc gatgatcgta ttcctccact tggttcattt atactcaaag
     9601 aatactcaat gtctttagtg tttagataac tttttgtaaa tcatatagat tgttttaaca
     9661 aaaaacaatt caatagtaga ttttcacatg aaagttacat aaaaattctt taaaattact
     9721 ttctcaaaaa attgttccaa acatattatc ccacaattaa actcaatctg tttttcgaaa
     9781 cctaaatcaa aaccaatcca actaccttat ataatatata atcaatacat tgtaaagaac
     9841 tgcatgttct tttaaatttt gggggcaaag ttattccgta cgttcacaca tgtactaata
     9901 ggaggtaata aatgatatgt gaaacaatcg aggtgtaaac aagctagcat tgagcaggcc
     9961 tcccagcttt cgtccgtatc atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt
    10021 tcattgccca cacaccagaa tcctactaag tttgagtatt atggcattgg aaaagctgtt
    10081 ttcttctatc atttgttctg cttgtaattt actgtgttct ttcagttttt gttttcggac
    10141 atcaaaatgc aaatggatgg ataagagtta ataaatgata tggtcctttt gttcattctc
    10201 aaattattat tatctgttgt ttttacttta atgggttgaa tttaagtaag aaaggaacta
    10261 acagtgtgat attaaggtgc aatgttagac atataaaaca gtctttcacc tctctttggt
    10321 tatgtcttga attggtttgt ttcttcactt atctgtgtaa tcaagtttac tatgagtcta
    10381 tgatcaagta attatgcaat caagttaagt acagtatagg ctttgtcatt ggcggaatct
    10441 ttgagaggga ataaaagaaa tacttccaaa aatccattag aagaactata aatcttctgg
    10501 gcatgtaaat ataacgtaaa atatatgtta tgttacaacc ttatttcatt cacattttaa
    10561 ttttttttca cacaattttt atttaatttt gtttttatct ctattttatt ttcatatgat
    10621 aatacatatt atctctttat tttactttat gtgtctcttt gctctcacat tttatttctt
    10681 aactaacata ttctcattag cagtagctat cgtaagacta atctcacaca ataattaatt
    10741 atccattcta tttatgaatt tttatcattt aataattctt tacctgtgca aattttgaag
    10801 gaaaaagttt ctgaatataa aataacaata atactaatta gatgtcaaat caaatcaccc
    10861 aggctagaca atagtggtaa tgaagaaggc tagaggtagc tctgtgcctc taggggagtg
    10921 agagacgttt ttcatttatt ggtgtgttga aacttgaaac gttgatgttc tccacagctc
    10981 cacgtattct ttcgtcctag aacgaactag agcaacataa tctgtctctt cctgaagctt
    11041 cattgttttt ctttccttct agcagcatct tcttaacaga cgaagaaggg actcagatgc
    11101 ttgcttgcat gcttccaaag tttggagctg aggagacttg tgctatttga gtctgtttta
    11161 gcattcgcaa gccccacacc ggataaacat acagaaatct gagtgtttat aaccaaatag
    11221 tcagcaacaa ggttcgaggg ggactatgtg agtcgtgttt tagagctaga aatagcaagt
    11281 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttg
    11341 gcaaaaattt tcagattttt tcttcatctg tagatttctg ggtttttttt tccgtttcgt
    11401 gaatcataag tgaagttttg gatgcaaatc tgcgcgaaaa aagttggacc tgcaatgagc
    11461 ttatttagat agctaagaca aagtgattgg tccgttgttt cagttctgat tgtcagagag
    11521 tttgtttcga gtcggcgaca ccaatgcgtt ttgttaacca gatttcgggt aagaaatgta
    11581 tcgagagttt gtttccagac ggctacatca ttttcttatg aagggtgaaa ttagatagac
    11641 caaagattga aacacaacat ttctttcaca aaaatataat aaacttgata gcatttagga
    11701 tcagccacct gacctaagct agcctagaag gaatctttga gagggaataa aagaaatact
    11761 tccaaaaatc cattagaaga actataaatc ttctgggcat gtaaatataa cgtaaaatat
    11821 atgttatgtt acaaccttat ttcattcaca ttttaatttt ttttcacaca atttttattt
    11881 aattttgttt ttatctctat tttattttca tatgataata catattatct ctttatttta
    11941 ctttatgtgt ctctttgctc tcacatttta tttcttaact aacatattct cattagcagt
    12001 agctatcgta agactaatct cacacaataa ttaattatcc attctattta tgaattttta
    12061 tcatttaata attctttacc tgtgcaaatt ttgaaggaaa aagtttctga atataaaata
    12121 acaataatac taattagatg tcaaatcaaa tcacccaggc tagacaatag tggtaatgaa
    12181 gaaggctaga ggtagctctg tgcctctagg ggagtgagag acgtttttca tttattggtg
    12241 tgttgaaact tgaaacgttg atgttctcca cagctccacg tattctttcg tcctagaacg
    12301 aactagagca acataatctg tctcttcctg aagcttcatt gtttttcttt ccttctagca
    12361 gcatcttctt aacagacgaa gaagggactc agatgcttgc ttgcatgctt ccaaagtttg
    12421 gagctgagga gacttgtgct atttgagtct gttttagcat tcgcaagccc cacaccggat
    12481 aaacatacag aaatctgagt gtttataacc aaatagtcag caacaaggtt cgatgagtac
    12541 cggaatcacc aggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
    12601 acttgaaaaa gtggcaccga gtcggtgctt tttttggcaa aaattttcag attttttctt
    12661 catctgtaga tttctgggtt tttttttccg tttcgtgaat cataagtgaa gttttggatg
    12721 caaatctgcg cgaaaaaagt tggacctgca atgagcttat ttagatagct aagacaaagt
    12781 gattggtccg ttgtttcagt tctgattgtc agagagtttg tttcgagtcg gcgacaccaa
    12841 tgcgttttgt taaccagatt tcgggtaaga aatgtatcga gagtttgttt ccagacggct
    12901 acatcatttt cttatgaagg gtgaaattag atagaccaaa gattgaaaca caacatttct
    12961 ttcacaaaaa tataataaac ttgatagcat ttaggatcag ctgtctgtcg cggggagaga
    13021 ggattttgag gaaataatta attgaattac ttgtattatt gataaagtaa tttagataag
    13081 ttgttagtac aacttattgc aactatggat agacaaaaat cacttatttt aaggggagaa
    13141 gtatataaca acttattata aaatttctga gcagccggcc tcctccaatc aatcatagag
    13201 agtggagccc attctggaaa accaaggaac ccccaacttg cactggtgcg gtggcccaat
    13261 tcaaaaaaaa cggggcccag caaagtaacg ccgttgtaac gatttatcaa tccaaactca
    13321 aaaggcggcg agatgcgttt aactccgtga aattaacaaa ccgccaacaa cttgcaattt
    13381 gcaactaccg tttccaagaa gaactcaacc acacaacgta tcctatccca aaccacacgc
    13441 aactagtgac gcgtcataag gacacgtgtc acaatttgac tggttaataa tttcaccgct
    13501 tttgctataa attacctcca atccccttag cttcttcaca attcagttcc caaccctaac
    13561 aattcttgtt catatcgctt ctctctactt tcaaggtatg atccaatttc tctcttcttt
    13621 ctctgtaatc ctttcgttga gttttgtttc cgatcaatca taggtagttt tcttgtttcg
    13681 aagcatgaga tctaggaatt ttttgtgatt ttccaaaatt gagatcggtt tgaaattgaa
    13741 ttttacagct tgaatctcag atcttgtttt atcaatgttt tcgatggctt gcgatgtaga
    13801 tctatgataa ttgtggttca gttttgttag gaatcgattt cggtttagca attgcagatt
    13861 aattagggtt tccaattgaa ttcttcagat ccgttatgga attatgtcaa ataatttatt
    13921 caaattggaa attattgtta gatccactct taatctgttt gatccaagct tcaattaggg
    13981 ttttcacttg tttcaatttc ttgttatgga ttctgattta tctgttgatg ttagatccac
    14041 tcttaatctg tttgatccaa gcgttaatta gggttttcac ttgtttcaat ttcgtgtgtt
    14101 ggattctgat ttatctgttg ttgatgtgat tacagtctga acaatgggca gccaccacca
    14161 ccaccaccac agcggaatga gttcactgcc gtataggcag ctaagaaaat caggtggcac
    14221 cgaagatgca aagaacatta agaaagggcc tgcaccattc tatccacttg aggacggtac
    14281 agccggtgag caattgcata aggccatgaa gagatatgca cttgtaccag ggacgatagc
    14341 ctttactgat gctcacattg aggtcaatat cacttatgcg gaatattttg aaatgtcagt
    14401 cagattggca gaagctatga aaaggtatgg cttaaatacc aatcacagaa ttgtggtgtg
    14461 ttctgagaat agtttacagt tcttcatgcc agtgttgggt gcactattta ttggtgtagc
    14521 tgttgctcca gctaacgaca tttacaatga gagagaacta ctcaactcta tgaatatttc
    14581 acagcccaca gtcgtatttg tgagcaagaa aggattgcag aagatactta acgttcagaa
    14641 gaaacttccg attatacaga agatcattat tatggacagc aaaactgact accaaggctt
    14701 tcagtctatg tatacatttg tgacatctca tttacctcca ggctttaatg agtatgactt
    14761 cgttccagaa tcatttgatc gtgataagac tatcgctctt attatgaata gctctggatc
    14821 cactggtctg ccgaagggag tagcattgcc tcataggaca gcttgtgtta ggttttcaca
    14881 cgcaagagat ccgatatttg gaaatcagat cattcctgat acggctatat tgagtgttgt
    14941 accatttcac catggctttg gaatgtttac cacacttggg tacttgattt gcggctttag
    15001 agtggtactg atgtacaggt tcgaggagga acttttccta cgaagtctcc aagattacaa
    15061 aatccaatcc gctttgttag ttcctacact gttttccttc ttcgcgaaat ctaccctgat
    15121 tgacaaatat gatctctcta atctccacga aatagcaagt ggtggtgcac ctctttcaaa
    15181 agaggttgga gaagcagttg ccaaacgttt tcatctacca ggtatcagac aaggatatgg
    15241 tcttactgag acaacatcag ccatattaat tacccctgaa ggcgatgata agcctggtgc
    15301 tgttggaaaa gttgttccat tcttcgaagc aaaagtggta gacttggata ctggcaagac
    15361 gcttggagtc aaccaaagag gtgaactgtg cgttagaggg cctatgatca tgtctgggta
    15421 cgttaacaat cctgaggcaa ccaatgcgtt aattgacaaa gatggctggt tacatagtgg
    15481 agatattgcg tactgggacg aagatgagca cttcttcata gtggataggt tgaagtctct
    15541 catcaagtac aaaggttatc aagtagctcc tgctgaatta gagtcaatcc ttcttcaaca
    15601 tcccaacatt ttcgatgctg gagtggccgg attgcccgat gatgatgccg gtgaattgcc
    15661 cgcagctgtt gttgtgctgg aacatggtaa gactatgaca gagaaggaaa tagtggacta
    15721 tgtcgctagc caagttacta ctgctaagaa actccgtgga ggggttgtct ttgttgatga
    15781 agtcccaaaa ggattgactg ggaaacttga tgctcgaaag ataagagaga ttctcattaa
    15841 agctaagaag ggtggaaaat ccaaactata aaagggctaa gacctaagct agcctagaat
    15901 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
    15961 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
    16021 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
    16081 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
    16141 tatgttacta gatctgagtg acctaagcta gcctagtgtc tgaggcggcc aacaaactat
    16201 tcgtcacgtc gacaaaattt agaacgaact taattatgat ctcaaataca ttgatacata
    16261 tctcatctag atctaggtta tcattatgta agaaagtttt gacgaatatg gcacgacaaa
    16321 atggctagac tcgatgtaat tggtatctca actcaacatt atacttatac caaacattag
    16381 ttagacaaaa tttaaacaac tattttttat gtatgcaaga gtcagcatat gtataattga
    16441 ttcagaatcg ttttgacgag ttcggatgta gtagtagcca ttatttaatg tacatactaa
    16501 tcgtgaatag tgaatatgat gaaacattgt atcttattgt ataaatatcc ataaacacat
    16561 catgaaagac actttctttc acggtctgaa ttaattatga tacaattcta atagaaaacg
    16621 aattaaatta cgttgaattg tatgaaatct aattgaacaa gccaaccacg acgacgacta
    16681 acgttgcctg gattgactcg gtttaagtta accactaaaa aaacggagct gtcatgtaac
    16741 acgcggatcg agcaggtcac agtcatgaag ccatcaaagc aaaagaacta atccaagggc
    16801 tgagatgatt aattagttta aaaattagtt aacacgaggg aaaaggctgt ctgacagcca
    16861 ggtcacgtta tctttacctg tggtcgaaat gattcgtgtc tgtcgatttt aattattttt
    16921 ttgaaaggcc gaaaataaag ttgtaagaga taaacccgcc tatataaatt catatatttt
    16981 cctctccgct ttgaattgtc tcgttgtcct cctcactttc atcagccgtt ttgaatctcc
    17041 ggcgacttga cagagaagaa caaggaagaa gtctaagaga gaaagtaaga gataatccag
    17101 gagattcatt ctccgttttg aatcttcctc aatctcatct tcttccgctc tttctttcca
    17161 aggtaatagg aactttctgg atctacttta tttgctggat ctcgatcttg ttttctcaat
    17221 ttccttgaga tctggaattc gtttaatttg gatctgtgaa cctccactaa atcttttggt
    17281 tttactagaa tcgatctaag ttgaccgatc agttagctcg attatagcta ccagaatttg
    17341 gcttgacctt gatggagaga tccatgttca tgttacctgg gaaatgattt gtatatgtga
    17401 attgaaatct gaactgttga agttagattg aatctgaaca ctgtcaatgt tagattgaat
    17461 ctgaacactg tttaaggtta gatgaagttt gtgtatagat tcttcgaaac tttaggattt
    17521 gtagtgtcgt acgttgaaca gaaagctatt tctgattcaa tcagggttta tttgactgta
    17581 ttgaactctt tttgtgtgtt tgcagctcat aaaaatctgc ccacgactca catagtcccc
    17641 ctcaaacacc atggatcatt atcttgatat tagacttaga cctgatcctg aatttcctcc
    17701 tgctcaactt atgtctgttc tttttggaaa gcttcatcaa gctcttgttg ctcaaggagg
    17761 agatagaatt ggagtttctt ttcctgatct tgatgaatct agatctagac ttggagaaag
    17821 acttagaatt catgcttctg ctgatgatct tagagctctt cttgctagac cttggcttga
    17881 aggacttaga gatcatcttc aatttggaga acctgctgtt gttcctcatc ctactcctta
    17941 tagacaagtt tctagagttc aagctaagtc taatcctgaa agacttagaa gaagattgat
    18001 gaggagacat gatctttctg aagaagaagc tagaaagaga attcctgata ctgttgctag
    18061 agctcttgat cttccttttg ttactcttag atctcaatct actggacaac attttagact
    18121 ttttattaga catggacctc ttcaagttac tgctgaagaa ggaggattta cttgttatgg
    18181 actttctaag ggaggatttg ttccttggtt ttgaaaggtt tgatgagtac cggaatcacc
    18241 aggggaatca tatgaagatg aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg
    18301 cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat
    18361 taaatgaatg taagatctca ttataatgaa taaacaaatg tttctataat ccattgtgaa
    18421 tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt atggactatg
    18481 gaatatgatt aaagataagt gagtgaccta agctagccta gtgtctctgt ctgtgagacc
    18541 tctggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
    18601 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
    18661 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
    18721 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccttcttct gaaaacgaca
    18781 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
    18841 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
    18901 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
    18961 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
    19021 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
    19081 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
    19141 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
    19201 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
    19261 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
    19321 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
    19381 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
    19441 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
    19501 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
    19561 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
    19621 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
    19681 cgcgcacatc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
    19741 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
    19801 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
    19861 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
    19921 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
    19981 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
    20041 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
    20101 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
    20161 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
    20221 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
    20281 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
    20341 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
    20401 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
    20461 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
    20521 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
    20581 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
    20641 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
    20701 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
    20761 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
    20821 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
    20881 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
    20941 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
    21001 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
    21061 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
    21121 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
    21181 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
    21241 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
    21301 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
    21361 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
    21421 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
    21481 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
    21541 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
    21601 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
    21661 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
    21721 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
    21781 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
    21841 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
    21901 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
    21961 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
    22021 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggactct
    22081 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
    22141 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
    22201 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
    22261 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
    22321 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
    22381 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
    22441 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
    22501 gatgacggtg aaaacctctg acacatgcag ctcccggaga cagtcacagc ttgtctgtaa
    22561 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggt
//
