LOCUS       pT7_CC_PE7_IVT_T       10862 bp DNA     circular SYN 14-JUL-2024
DEFINITION  Template for in vitro transcription of PE7. For HSPC-related 
            experiments.
ACCESSION   urn.local...5r-gq123u8
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y,
            Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, 
            Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B.
  TITLE     Improving prime editing with an endogenous small RNA-binding 
            protein.
  JOURNAL   Nature 2024 Apr;628:639-647
  PUBMED    38570691
REFERENCE   2  (bases 1 to 10862)
  AUTHORS   Levesque S, Cosentino A, Verma A, Genovese P, Bauer DE.
  TITLE     Enhancing prime editing in hematopoietic stem and progenitor cells 
            by modulating nucleotide metabolism.
  JOURNAL   Nat Biotechnol 2024 May 28.
  PUBMED    38806736
REFERENCE   3  (bases 1 to 10862)
  AUTHORS   Sebastien Levesque
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07259-6"; journalName: "Nature"; date: 
            "2024-04"; volume: "628"; pages: "639-647"
COMMENT     SGRef: number: 2; type: "Journal Article"; doi: 
            "10.1038/s41587-024-02266-4"; journalName: "Nat Biotechnol 2024 May 
            28."
FEATURES             Location/Qualifiers
     source          1..10862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /standard_name="CMV promoter"
                     /label=CMV promoter
                     /note="<html><body>human cytomegalovirus (CMV) immediate
                     early promoter</body></html>"
     primer_bind     286..306
                     /standard_name="CMV-F"
                     /label=CMV-F
     promoter        377..395
                     /standard_name="T7 promoter"
                     /label=T7 promoter
                     /note="<html><body>promoter for bacteriophage T7 RNA 
                     polymerase</body></html>"
     primer_bind     377..393
                     /standard_name="T7"
                     /label=T7
     regulatory      402..411
                     /standard_name="Kozak"
                     /label=Kozak
     CDS             408..464
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             465..4565
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from 
                     theStreptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(R221K N394K H840A)
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4566..4667
                     /label=SGGSx2-sv40NLS-SGGSx2
     CDS             4668..6698
                     /standard_name="M-MLV reverse transcriptase"
                     /label=MMLV-RT codon optimized Genscript
     misc_feature    5300..5303
                     /standard_name="Ligation"
                     /label=nonstandard type: ligation
     CDS             6699..6794
                     /codon_start=1
                     /standard_name="2XSGGS XTEN 2XSGGS"
                     /label=2XSGGS XTEN 2XSGGS
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             6795..7376
                     /codon_start=1
                     /standard_name="SSB ensembl 1-194"
                     /label=La (SSB) 1-194
                     /translation="MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEG
                     WVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEY
                     KNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIES
                     AKKFVETPGQKYKETDLLILFKDDYFAKKNE"
     CDS             7389..7442
                     /codon_start=1
                     /standard_name="Bipartite SV40 NLS"
                     /label=Bipartite SV40 NLS
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             7443..7478
                     /codon_start=1
                     /standard_name="GSG-cmycNLS"
                     /label=GSG-cmycNLS
                     /translation="GSGPAAKRVKLD"
     misc_feature    7482..7485
                     /standard_name="Ligation"
                     /label=nonstandard type: ligation
     misc_feature    7497..7631
                     /standard_name="HBB UTR"
                     /label=HBB UTR
                     /note="/vntifkey=21"
     misc_feature    7643..7777
                     /standard_name="HBB UTR"
                     /label=HBB UTR
                     /note="/vntifkey=21"
     polyA_site      7789..7878
     misc_feature    complement(7917..7934)
                     /direction=LEFT
                     /standard_name="BGH-rev"
                     /label=nonstandard type: primer_bind_reverse
     polyA_signal    join(7923..8123,8124..8147)
                     /note="<html><body>bovine growth hormone polyadenylation 
                     signal</body></html>"
     misc_feature    complement(8094..8133)
                     /direction=LEFT
                     /standard_name="polyA_extension_Rv"
                     /label=nonstandard type: primer_bind_reverse
     primer_bind     complement(8218..8234)
                     /standard_name="M13 rev"
                     /label=M13 rev
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
     protein_bind    8242..8258
                     /standard_name="lac operator"
                     /label=lac operator binding site
                     /bound_moiety="<html><body><i>lac</i> repressor encoded 
                     by<i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the 
                     <i>lac</i> operator to inhibit transcription in <i>E. 
                     coli</i>. This inhibition can be relieved by adding lactose
                     or isopropyl-beta-D-thiogalactopyranoside 
                     (IPTG).</body></html>"
     promoter        complement(join(8266..8272,8273..8290,8291..8296))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8311..8332
                     /standard_name="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
     rep_origin      complement(8620..9208)
                     /direction=LEFT
     CDS             complement(join(9379..10170,10171..10239))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(join(10171..10239,9379..10170))
                     /codon_start=1
                     /gene="<html><body><i>bla</i></body></html>"
                     /standard_name="AmpR"
                     /product="<html><body>beta-lactamase</body></html>"
                     /label=AmpR
                     /note="<html><body>confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics</body></html>"
                     /translation="MPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*MSIQHFRVALIPFFAAF
                     CLPVFA"
     promoter        complement(10240..10344)
                     /gene="<html><body><i>bla</i></body></html>"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
     enhancer        join(10614..10862,1..131)
                     /standard_name="CMV enhancer"
                     /label=CMV enhancer
                     /note="<html><body>human cytomegalovirus immediate early 
                     enhancer</body></html>"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact ataaggagac tgccaagatg aaacggacag
      421 ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcgacaag aagtacagca
      481 tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
      541 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
      601 tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa
      661 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
      721 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
      781 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
      841 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
      901 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
      961 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
     1021 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
     1081 acgccaaggc catcctgtct gccagactga gcaagagcag aaagctggaa aatctgatcg
     1141 cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg
     1201 gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga
     1261 gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg
     1321 ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga
     1381 gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg
     1441 agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt
     1501 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag
     1561 ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg
     1621 aggaactgct cgtgaagctg aagagagagg acctgctgcg gaagcagcgg accttcgaca
     1681 acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg
     1741 aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc
     1801 gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca
     1861 gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt
     1921 ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg
     1981 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag
     2041 tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg
     2101 ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg
     2161 actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt
     2221 tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc
     2281 tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg
     2341 aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag
     2401 tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga
     2461 tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg
     2521 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg
     2581 acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc
     2641 tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc
     2701 tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga
     2761 accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg
     2821 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc
     2881 agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg
     2941 aactggacat caaccggctg tccgactacg atgtggacgc tatcgtgcct cagagctttc
     3001 tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga
     3061 gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc
     3121 tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg
     3181 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga
     3241 tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg
     3301 acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc
     3361 ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg
     3421 cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg
     3481 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc
     3541 aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg aactttttca
     3601 agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg
     3661 gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc
     3721 tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca
     3781 aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg
     3841 accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg
     3901 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca
     3961 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct
     4021 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg
     4081 aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg
     4141 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg
     4201 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg
     4261 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc
     4321 tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg
     4381 agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact
     4441 ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc
     4501 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag
     4561 gtgactccgg cggaagctct ggtggcagca agcggaccgc cgacggctct gaattcgaga
     4621 gccctaagaa gaaaagaaag gtgagcggag gctctagcgg cggaagcacc ctgaacattg
     4681 aggacgagta tagactgcat gaaacaagca aggaacccga cgtgtccctg ggctccacct
     4741 ggctgtccga ctttccccag gcctgggccg agacaggagg aatgggcctg gccgtgcggc
     4801 aggcacccct gatcatccct ctgaaggcca cctctacacc cgtgagcatc aagcagtacc
     4861 ctatgtctca ggaggccaga ctgggcatca agcctcacat ccagaggctg ctggaccagg
     4921 gcatcctggt gccatgccag agcccctgga acacaccact gctgcccgtg aagaagccag
     4981 gcaccaatga ctatagaccc gtgcaggatc tgagagaggt gaacaagagg gtggaggata
     5041 tccaccccac cgtgcccaac ccttacaatc tgctgtccgg cctgccccct tctcaccagt
     5101 ggtatacagt gctggacctg aaggatgcct tcttttgtct gagactgcac cctaccagcc
     5161 agccactgtt cgcctttgag tggagggacc ctgagatggg catctctggc cagctgacct
     5221 ggacacgcct gcctcagggc ttcaagaata gcccaacact gtttaacgag gccctgcacc
     5281 gcgacctggc agatttccgg atccagcacc cagatctgat cctgctgcag tacgtggacg
     5341 atctgctgct ggccgccacc agcgagctgg attgccagca gggaacacgc gccctgctgc
     5401 agaccctggg aaacctggga tatagggcat ccgccaagaa ggcccagatc tgtcagaagc
     5461 aggtgaagta cctgggctat ctgctgaagg agggccagag atggctgaca gaggccagga
     5521 aggagacagt gatgggccag ccaacaccca agaccccaag acagctgagg gagttcctgg
     5581 gcaaagcagg attttgcagg ctgttcatcc caggattcgc agagatggca gcacctctgt
     5641 acccactgac caagccgggc accctgttta attggggccc tgaccagcag aaggcctatc
     5701 aggagatcaa gcaggccctg ctgacagcac cagccctggg cctgccagac ctgaccaagc
     5761 ctttcgagct gtttgtggat gagaagcagg gctacgccaa gggcgtgctg acccagaagc
     5821 tgggaccatg gagacggccc gtggcctatc tgtccaagaa gctggaccca gtggcagcag
     5881 gatggccacc atgcctgagg atggtggcag caatcgccgt gctgacaaag gatgccggca
     5941 agctgaccat gggacagcca ctggtcatcc tggcaccaca cgcagtggag gccctggtga
     6001 agcagcctcc agatcgctgg ctgtctaacg cccggatgac acactaccag gccctgctgc
     6061 tggacaccga tcgcgtgcag tttggccctg tggtggccct gaatccagcc accctgctgc
     6121 ctctgccaga ggagggcctg cagcacaact gtctggacat cctggcagag gcacacggaa
     6181 caaggccaga cctgaccgat cagcccctgc ctgacgccga tcacacatgg tataccgatg
     6241 gaagctccct gctgcaggag ggccagagga aggcaggagc agcagtgacc acagagacag
     6301 aagtgatctg ggccaaggcc ctgccagcag gcacatccgc ccagcgggcc gagctgatcg
     6361 ccctgaccca ggccctgaag atggccgagg gcaagaagct gaacgtgtac acagactcca
     6421 gatatgcctt cgccaccgca cacatccacg gagagatcta caggcgccgg ggctggctga
     6481 cctctgaggg caaggagatc aagaacaagg atgagatcct ggccctgctg aaggccctgt
     6541 ttctgcccaa gcggctgagc atcatccact gtcctggaca ccagaaggga cactccgccg
     6601 aggcaagggg caatcggatg gccgaccagg ccgccagaaa ggctgctatt actgaaactc
     6661 ccgacacttc cactctgctg attgaaaact cctccccttc cggaggatct agcggaggct
     6721 cctctggctc tgagacacct ggcacaagcg agagcgcaac acctgaaagc agcgggggca
     6781 gcagcggggg gtcaatggct gaaaatggtg ataatgaaaa gatggctgcc ctggaggcca
     6841 aaatctgtca tcaaattgag tattattttg gcgacttcaa tttgccacgg gacaagtttc
     6901 taaaggaaca gataaaactg gatgaaggct gggtaccttt ggagataatg ataaaattca
     6961 acaggttgaa ccgtctaaca acagacttta atgtaattgt ggaagcattg agcaaatcca
     7021 aggcagaact catggaaatc agtgaagata aaactaaaat cagaaggtct ccaagcaaac
     7081 ccctacctga agtgactgat gagtataaaa atgatgtaaa aaacagatct gtttatatta
     7141 aaggcttccc aactgatgca actcttgatg acataaaaga atggttagaa gataaaggtc
     7201 aagtactaaa tattcagatg agaagaacat tgcataaagc atttaaggga tcaatttttg
     7261 ttgtgtttga tagcattgaa tctgctaaga aatttgtaga gacccctggc cagaagtaca
     7321 aagaaacaga cctgctaata cttttcaagg acgattactt tgccaaaaaa aatgaatctg
     7381 gcggctcaaa aagaaccgcc gacggcagcg aattcgagtc tcccaagaag aagaggaaag
     7441 tcggctctgg ccctgccgct aagagagtga agctggacta accggtcccg ggtctagaag
     7501 ctcgctttct tgctgtccaa tttctattaa aggttccttt gttccctaag tccaactact
     7561 aaactggggg atattatgaa gggccttgag catctggatt ctgcctaata aaaaacattt
     7621 attttcattg ctcatgcata tagaagctcg ctttcttgct gtccaatttc tattaaaggt
     7681 tcctttgttc cctaagtcca actactaaac tgggggatat tatgaagggc cttgagcatc
     7741 tggattctgc ctaataaaaa acatttattt tcattgctct tagctaataa aaaaaaaaaa
     7801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     7861 aaaaaaaaaa aaaaaaaagt cttcactagt aagctgctga taaacccgct gatcagcctc
     7921 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7981 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8041 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8101 ttgggaagtc aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     8161 aagaaccagc tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8221 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8281 aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt
     8341 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8461 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8521 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8581 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8641 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8701 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8761 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8821 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8881 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8941 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9001 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9061 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9121 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9181 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9241 cactcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9301 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9361 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9421 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9481 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9541 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9601 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9661 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9721 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9781 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9841 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9901 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9961 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10021 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10081 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10141 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10201 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10261 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10321 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10381 atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc
    10441 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    10501 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
    10561 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
    10621 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
    10681 tgagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
    10741 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
    10801 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    10861 tc
//
