LOCUS       Rag2-LYZ-CE/pISceI       12761 bp    DNA     circular     10-JUN-2024
DEFINITION  .
ACCESSION   urn.local...1c-htpwsm6
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|610539508|                                                 
            VNTDBDATE|610539508|                                               
            LSOWNER|                                                           
            VNTNAME|rag2-YEATS4/pISceI|                                        
            VNTAUTHORNAME|Raja Ali|                                            
            VNTAUTHOREML|raja.ali@childrens.harvard.edu|                       
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
            (SXF                                                               
             (CGexDoc "rag2-YEATS4/pISceI" 0 13245                             
              (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList)
            (CObList)     (CObList) -1 "")                                     
                         (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10
            10 4294967295 50 0     1 0 (CHomObj 0 0 0 3 75) (CWordArray)
            (CWordArray)                  (CStringList "ApaLI" "AvaI" "BamHI"
            "ClaI" "EcoRI" "HindIII" "NcoI"      "PstI" "SmaI" "XmaI")
            (CStringList "atg" "gtg")                    (CStringList "taa"
            "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0     4294967295 0 1 0
            0 0 0 0 "MAIN" 0 0 30 0                           
            (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0))        
            (CMolPar 0 0 0 0 0 1 13245 0 0 0 0 0 0 0 0) (CStringList)
            (CStringList)    (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25
            250 50 0 6 4 3 7)    (COAPar 25 250 50 0 6 4 3 7)                  
                                 (CObList                                      
                                  #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0
            2 10882 0 12128 0 0 "")     #1=(CRSite (CStringList) "AvaI"
            "cycgrg" 2 0 2 7236 0 9391 0 0 "")     #2=(CRSite (CStringList)
            "BamHI" "ggatcc" 2 0 1 6507 0 0 "")        #3=(CRSite (CStringList)
            "ClaI" "atcgat" 3 0 1 7434 0 0 "")         #4=(CRSite (CStringList)
            "EcoRI" "gaattc" 2 0 1 2627 0 0 "")        #5=(CRSite (CStringList)
            "HindIII" "aagctt" 2 0 1 7763 0 0 "")      #6=(CRSite (CStringList)
            "NcoI" "ccatgg" 2 0 3 6403 0 6519 0 8659 0 0 "")     #7=(CRSite
            (CStringList) "PstI" "ctgcag" 6 0 5 3888 0 4571 0 7083 0 7392      
              0 8557 0 0 "") #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0
            "")     #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 ""))      
                   (CObList                                                    
                    #10=(CFSignal (CObList) "zrag2 promoter" 29 0 0 1 6505 0
            (CStringList)          (CStringList) 1 1 1 1 "1..6505")            
                              #11=(CFSignal (CObList) "I-SceI (Rev)" 87 0 1
            9946 9963 0 (CStringList)          (CStringList) 1 1 1 1
            "complement(9366..9383)")                #12=(CFSignal (CObList)
            "I-SceI (Rev)" 87 0 1 12859 12876 0              (CStringList)
            (CStringList) 1 1 1 1 "complement(12279..12296)")     #13=(CFSignal
            (CObList) "Ampicillin-R" 22 0 0 10766 11626 0             
            (CStringList) (CStringList) 1 1 1 1 "10186..11046")           
            #14=(CFSignal (CObList) "Ori" 33 0 1 11771 12444 0 (CStringList)   
                  (CStringList) 1 1 1 1 "complement(11191..11864)")            
             #15=(CFSignal (CObList) "SV40 pA" 25 0 0 7445 7575 0 (CStringList)
                     (CStringList) 1 1 1 1 "6865..6995")                       
                #16=(CFSignal (CObList) "SV40 late polyA (from pCS2+)" 25 0 1
            9618 9815          0 (CStringList)                                 
                               (CStringList "/SerialCloner_Color=&hFF80FF"     
                                "/SerialCloner_Show=True"
            "/SerialCloner_Protect=True"             
            "/SerialCloner_Arrow=True") 1 1 1 1 "complement(9038..9235)")    
            #17=(CFSignal (CObList) "cmlc2 promoter" 30 0 0 7761 8659 0        
                 (CStringList)                                                 
                 (CStringList "/SerialCloner_Color=&hFF8080"                   
                  "/SerialCloner_Show=True" "/SerialCloner_Protect=True"       
                  "/SerialCloner_Arrow=True") 1 1 1 1 "7181..8079")            
            #18=(CFSignal (CObList) "EGFP [*]" 4 0 0 8660 9376 0 (CStringList) 
                    (CStringList "/SerialCloner_Color=&h00FF00"                
                     "/SerialCloner_Show=True" "/SerialCloner_Protect=True"    
                     "/SerialCloner_Arrow=True") 1 1 1 1 "8080..8796")         
               #19=(CFSignal (CObList) "EGFP-like chromophore" 21 0 1 8851 8864
            0          (CStringList)                                           
                       (CStringList "/SerialCloner_Color=&h84A4C0"             
                        "/SerialCloner_Show=True" "/SerialCloner_Protect=True" 
                        "/SerialCloner_Arrow=True") 1 1 1 1
            "complement(8271..8284)")     #20=(CFSignal (CObList) "mutated
            BseRI restriction site" 21 0 1 8675          8680 0 (CStringList)  
                                                         (CStringList
            "/SerialCloner_Color=&hFF0080"                         
            "/SerialCloner_Show=True" "/SerialCloner_Protect=True"             
            "/SerialCloner_Arrow=True") 1 1 1 1 "complement(8095..8100)")    
            #21=(CFSignal (CObList) "CDK4" 4 0 0 6520 7431 0 (CStringList)     
                 (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList)
            (CObList)    (CObList) (CObList)                                   
                         (CTextView 0                                          
                          #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180)      
                               (CObjectList                                    
                                #23=(CRefLinePar                               
                                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233)
                                      "rag2-YEATS4/pISceI" 2) 5 "" 0 4)        
                                #24=(CFolderPar                                
                                     (CGroupPar (CParagraph 1 (0 0) 1 1 0 0
            178)                          (CObjectList                         
                                           #25=(CLinePar (CParagraph 0 (0 0) 1
            2 1 0 180)                           "DNA 'rag2-YEATS4/pISceI'" 1) 
                                            #26=(CLinePar (CParagraph 0 (0 0) 1
            2 1 0 180)                           "Local object" 1)             
                                            #27=(CLinePar (CParagraph 0 (0 0) 1
            2 1 0 180)                           "Created: 12/26/11 10:18AM" 1)
                                            #28=(CLinePar (CParagraph 0 (0 0) 1
            2 1 0 180)                           "Last Modified: 12/26/11
            10:18AM" 1)                           #29=(CLinePar (CParagraph 0
            (0 0) 1 2 1 0 180)                           "length: 13245 bp" 1) 
                                                    #30=(CLinePar (CParagraph 0
            (0 0) 1 2 1 0 180)                           "storage type: Basic"
            1)                                       #31=(CLinePar (CParagraph
            0 (0 0) 1 2 1 0 180)                           "form: Circular"
            1))) "General Description")            #32=(CFolderPar             
                                                        (CGroupPar (CParagraph
            2 (0 0) 1 1 0 0 178)                          (CObjectList         
                                                           #33=(CLinePar
            (CParagraph 0 (0 0) 1 2 1 0 180)                          
            "Original Source Database: GenBank" 1)                        
            #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)                     
                 "Modification Date in the Original DB: 26-DEC-2011" 1)))      
                     "Standard Fields")                                        
                #35=(CRefLinePar                                               
                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1
            ""                0 0)                                             
                         #36=(CFolderPar                                       
                              (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178)
            (CObjectList))                "Annotations")                       
                                     #37=(CFolderPar                           
                                          (CGroupPar (CParagraph 12 (6 0) 1 1 0
            0 178)                         (CObjectList                        
                                            #38=(CFolderPar                    
                                                 (CGroupPar (CParagraph 4 (7 4
            0) 1 1 1 0 178)                        (CObjectList                
                                                    #39=(CFolderPar            
                                                         (CGroupPar            
                                                          (CParagraph 6520 (3
            #21# 0) 1 2 2 0 327)                               (CObjectList    
                                                                #40=(CLinePar  
                                                                    
            (CParagraph 0 (0 0) 1 2 3 0 180)                                   
             "Start: 6520  End: 7431" 1))) "CDK4")                        
            #41=(CFolderPar                                                    
                 (CGroupPar                                                    
                  (CParagraph 8660 (3 #18# 0) 1 2 2 0 327)                     
                     (CObjectList                                              
                      #42=(CLinePar                                            
                           (CParagraph 0 (0 0) 1 2 3 0 180)                    
                            "Start: 8660  End: 9376" 1)                        
                       #43=(CLinePar                                           
                            (CParagraph 0 (0 0) 1 2 3 0 180)                   
                             "Original Location Description:" 1)               
                            #44=(CLinePar                                      
                                 (CParagraph 0 (0 0) 1 2 3 0 180)              
                                  "   8080..8796" 1)                           
                             #45=(CLinePar                                     
                                  (CParagraph 0 (0 0) 1 2 3 0 180)             
                                   "Qualifiers:" 1)                            
                              #46=(CLinePar                                    
                                   (CParagraph 0 (0 0) 1 2 3 0 180)            
                                    "   /SerialCloner_Color=&h00FF00" 1)       
                                    #47=(CLinePar                              
                                         (CParagraph 0 (0 0) 1 2 3 0 180)      
                                          "   /SerialCloner_Show=True" 1)      
                                     #48=(CLinePar                             
                                          (CParagraph 0 (0 0) 1 2 3 0 180)     
                                           "   /SerialCloner_Protect=True" 1)  
                                         #49=(CLinePar                         
                                              (CParagraph 0 (0 0) 1 2 3 0 180) 
                                               "   /SerialCloner_Arrow=True"
            1)))                              "EGFP [*]"))) "CDS (2 total)")   
                                  #50=(CFolderPar                              
                                       (CGroupPar (CParagraph 21 (7 21 0) 1 1 1
            0 178)                        (CObjectList                         
                                           #51=(CFolderPar                     
                                                (CGroupPar                     
                                                 (CParagraph 8675 (3 #20# 0) 1
            2 2 0 194)                               (CObjectList              
                                                      #52=(CLinePar            
                                                           (CParagraph 0 (0 0)
            1 2 3 0 180)                                     "Start: 8675  End:
            8680 (Complementary)"                                     1)       
                                                                 #53=(CLinePar 
                                                                     
            (CParagraph 0 (0 0) 1 2 3 0 180)                                   
             "Original Location Description:" 1)                               
            #54=(CLinePar                                                      
                 (CParagraph 0 (0 0) 1 2 3 0 180)                              
                  "   complement(8095..8100)" 1)                               
             #55=(CLinePar                                                     
                  (CParagraph 0 (0 0) 1 2 3 0 180)                             
                   "Qualifiers:" 1)                                            
              #56=(CLinePar                                                    
                   (CParagraph 0 (0 0) 1 2 3 0 180)                            
                    "   /SerialCloner_Color=&hFF0080" 1)                       
                    #57=(CLinePar                                              
                         (CParagraph 0 (0 0) 1 2 3 0 180)                      
                          "   /SerialCloner_Show=True" 1)                      
                     #58=(CLinePar                                             
                          (CParagraph 0 (0 0) 1 2 3 0 180)                     
                           "   /SerialCloner_Protect=True" 1)                  
                         #59=(CLinePar                                         
                              (CParagraph 0 (0 0) 1 2 3 0 180)                 
                               "   /SerialCloner_Arrow=True" 1)))              
                           "mutated BseRI restriction site")                   
                      #60=(CFolderPar                                          
                           (CGroupPar                                          
                            (CParagraph 8851 (3 #19# 0) 1 2 2 0 194)           
                               (CObjectList                                    
                                #61=(CLinePar                                  
                                     (CParagraph 0 (0 0) 1 2 3 0 180)          
                                      "Start: 8851  End: 8864 (Complementary)" 
                                               1)                              
                                          #62=(CLinePar                        
                                               (CParagraph 0 (0 0) 1 2 3 0 180)
                                                "Original Location
            Description:" 1)                                #63=(CLinePar      
                                                                 (CParagraph 0
            (0 0) 1 2 3 0 180)                                     "  
            complement(8271..8284)" 1)                                
            #64=(CLinePar                                                      
                 (CParagraph 0 (0 0) 1 2 3 0 180)                              
                  "Qualifiers:" 1)                                             
             #65=(CLinePar                                                     
                  (CParagraph 0 (0 0) 1 2 3 0 180)                             
                   "   /SerialCloner_Color=&h84A4C0" 1)                        
                   #66=(CLinePar                                               
                        (CParagraph 0 (0 0) 1 2 3 0 180)                       
                         "   /SerialCloner_Show=True" 1)                       
                    #67=(CLinePar                                              
                         (CParagraph 0 (0 0) 1 2 3 0 180)                      
                          "   /SerialCloner_Protect=True" 1)                   
                        #68=(CLinePar                                          
                             (CParagraph 0 (0 0) 1 2 3 0 180)                  
                              "   /SerialCloner_Arrow=True" 1)))               
                          "EGFP-like chromophore")))                           
                   "Misc. Feature (2 total)")                                  
              #69=(CFolderPar                                                  
                   (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178)             
                      (CObjectList                                             
                       #70=(CFolderPar                                         
                            (CGroupPar                                         
                             (CParagraph 10766 (3 #13# 0) 1 2 2 0 194)         
                                 (CObjectList                                  
                                  #71=(CLinePar                                
                                       (CParagraph 0 (0 0) 1 2 3 0 180)        
                                        "Start: 10766  End: 11626" 1)          
                                   #72=(CLinePar                               
                                        (CParagraph 0 (0 0) 1 2 3 0 180)       
                                         "Original Location Description:" 1)   
                                        #73=(CLinePar                          
                                             (CParagraph 0 (0 0) 1 2 3 0 180)  
                                              "   10186..11046" 1)))
            "Ampicillin-R")))                       "Misc. Marker (1 total)")  
                                               #74=(CFolderPar                 
                                                    (CGroupPar (CParagraph 25
            (7 25 0) 1 1 1 0 178)                        (CObjectList          
                                                          #75=(CFolderPar      
                                                               (CGroupPar      
                                                                (CParagraph
            7445 (3 #15# 0) 1 2 2 0 194)                              
            (CObjectList                                                       
             #76=(CLinePar                                                     
                  (CParagraph 0 (0 0) 1 2 3 0 180)                             
                   "Start: 7445  End: 7575" 1)                                 
              #77=(CLinePar                                                    
                   (CParagraph 0 (0 0) 1 2 3 0 180)                            
                    "Original Location Description:" 1)                        
                   #78=(CLinePar                                               
                        (CParagraph 0 (0 0) 1 2 3 0 180)                       
                         "   6865..6995" 1))) "SV40 pA")                       
             #79=(CFolderPar                                                   
                  (CGroupPar                                                   
                   (CParagraph 9618 (3 #16# 0) 1 2 2 0 194)                    
                      (CObjectList                                             
                       #80=(CLinePar                                           
                            (CParagraph 0 (0 0) 1 2 3 0 180)                   
                             "Start: 9618  End: 9815 (Complementary)"          
                                      1)                                       
                                 #81=(CLinePar                                 
                                      (CParagraph 0 (0 0) 1 2 3 0 180)         
                                       "Original Location Description:" 1)     
                                      #82=(CLinePar                            
                                           (CParagraph 0 (0 0) 1 2 3 0 180)    
                                            "   complement(9038..9235)" 1)     
                                       #83=(CLinePar                           
                                            (CParagraph 0 (0 0) 1 2 3 0 180)   
                                             "Qualifiers:" 1)                  
                                        #84=(CLinePar                          
                                             (CParagraph 0 (0 0) 1 2 3 0 180)  
                                              "   /SerialCloner_Color=&hFF80FF"
            1)                                #85=(CLinePar                    
                                                   (CParagraph 0 (0 0) 1 2 3 0
            180)                                     "  
            /SerialCloner_Show=True" 1)                               
            #86=(CLinePar                                                      
                 (CParagraph 0 (0 0) 1 2 3 0 180)                              
                  "   /SerialCloner_Protect=True" 1)                           
                #87=(CLinePar                                                  
                     (CParagraph 0 (0 0) 1 2 3 0 180)                          
                      "   /SerialCloner_Arrow=True" 1)))                       
                  "SV40 late polyA (from pCS2+)")))                           
            "PolyA Signal (2 total)")                                     
            #88=(CFolderPar                                                    
                 (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178)               
                    (CObjectList                                               
                     #89=(CFolderPar                                           
                          (CGroupPar                                           
                           (CParagraph 1 (3 #10# 0) 1 2 2 0 194)               
                           (CObjectList                                        
                            #90=(CLinePar                                      
                                 (CParagraph 0 (0 0) 1 2 3 0 180)              
                                  "Start: 1     End: 6505" 1)                  
                             #91=(CLinePar                                     
                                  (CParagraph 0 (0 0) 1 2 3 0 180)             
                                   "Original Location Description:" 1)         
                                  #92=(CLinePar                                
                                       (CParagraph 0 (0 0) 1 2 3 0 180)        
                                        "   1..6505" 1))) "zrag2 promoter")))  
                                "Promoter Eukaryotic (1 total)")               
                           #93=(CFolderPar                                     
                                (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178)
                                   (CObjectList                                
                                    #94=(CFolderPar                            
                                         (CGroupPar                            
                                          (CParagraph 7761 (3 #17# 0) 1 2 2 0
            194)                               (CObjectList                    
                                                #95=(CLinePar                  
                                                     (CParagraph 0 (0 0) 1 2 3
            0 180)                                     "Start: 7761  End: 8659"
            1)                                    #96=(CLinePar                
                                                       (CParagraph 0 (0 0) 1 2
            3 0 180)                                     "Original Location
            Description:" 1)                                #97=(CLinePar      
                                                                 (CParagraph 0
            (0 0) 1 2 3 0 180)                                     "  
            7181..8079" 1)                                            
            #98=(CLinePar                                                      
                 (CParagraph 0 (0 0) 1 2 3 0 180)                              
                  "Qualifiers:" 1)                                             
             #99=(CLinePar                                                     
                  (CParagraph 0 (0 0) 1 2 3 0 180)                             
                   "   /SerialCloner_Color=&hFF8080" 1)                        
                   #100=(CLinePar                                              
                         (CParagraph 0 (0 0) 1 2 3 0 180)                      
                           "   /SerialCloner_Show=True" 1)                     
                      #101=(CLinePar                                           
                            (CParagraph 0 (0 0) 1 2 3 0 180)                   
                              "   /SerialCloner_Protect=True" 1)               
                            #102=(CLinePar                                     
                                  (CParagraph 0 (0 0) 1 2 3 0 180)             
                                    "   /SerialCloner_Arrow=True" 1)))         
                                "cmlc2 promoter")))                            
                         "Promoter Prokaryotic (1 total)")                     
                    #103=(CFolderPar                                           
                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178)      
                              (CObjectList                                     
                               #104=(CFolderPar                                
                                     (CGroupPar                                
                                      (CParagraph 11771 (3 #14# 0) 1 2 2 0 194)
                                            (CObjectList                       
                                             #105=(CLinePar                    
                                                   (CParagraph 0 (0 0) 1 2 3 0
            180)                                        "Start: 11771  End:
            12444 (Complementary)"                                        1)   
                                                                   
            #106=(CLinePar                                                     
                  (CParagraph 0 (0 0) 1 2 3 0 180)                             
                      "Original Location Description:" 1)                      
                       #107=(CLinePar                                          
                             (CParagraph 0 (0 0) 1 2 3 0 180)                  
                                 "   complement(11191..11864)" 1)))            
                               "Ori"))) "Replication Origin (1 total)")        
                     #108=(CFolderPar                                          
                           (CGroupPar (CParagraph 87 (7 87 0) 1 1 1 0 178)     
                               (CObjectList                                    
                                #109=(CFolderPar                               
                                      (CGroupPar                               
                                       (CParagraph 9946 (3 #11# 0) 1 2 2 0 194)
                                            (CObjectList                       
                                             #110=(CLinePar                    
                                                   (CParagraph 0 (0 0) 1 2 3 0
            180)                                        "Start: 9946  End: 9963
            (Complementary)"                                        1)         
                                                              #111=(CLinePar   
                                                                    (CParagraph
            0 (0 0) 1 2 3 0 180)                                       
            "Original Location Description:" 1)                                
             #112=(CLinePar                                                    
                   (CParagraph 0 (0 0) 1 2 3 0 180)                            
                       "   complement(9366..9383)" 1)))                        
                   "I-SceI (Rev)")                                             
             #113=(CFolderPar                                                  
                   (CGroupPar                                                  
                    (CParagraph 12859 (3 #12# 0) 1 2 2 0 194)                  
                          (CObjectList                                         
                           #114=(CLinePar                                      
                                 (CParagraph 0 (0 0) 1 2 3 0 180)              
                                     "Start: 12859  End: 12876 (Complementary)"
                                                   1)                          
                                             #115=(CLinePar                    
                                                   (CParagraph 0 (0 0) 1 2 3 0
            180)                                        "Original Location
            Description:" 1)                                  #116=(CLinePar   
                                                                    (CParagraph
            0 (0 0) 1 2 3 0 180)                                        "  
            complement(12279..12296)" 1)))                               
            "I-SceI (Rev)"))) "Misc. Signal (2 total)")))               
            "Feature Map")                                                
            #117=(CFolderPar                                                   
                  (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178)                 
                   (CObjectList                                                
                    #118=(CRSFolderPar                                         
                          (CFolderPar                                          
                           (CGroupPar (CParagraph 111381520 (8 0) 1 1 1 0 178) 
                                     (CObjectList                              
                                      #119=(CGroupPar                          
                                            (CParagraph 0 (10 #0# 0) 1 2 2 0
            180)                                  (CObjectList                 
                                                   #120=(CLinePar              
                                                         (CParagraph 0 (1 #0#
            1) 1 2 2 0 191)                                         " N1: 10882
            " 1)                                              #121=(CLinePar   
                                                                    (CParagraph
            0 (1 #0# 2) 1 2 2 0 191)                                         "
            N2: 12128 " 1)))))                                 "ApaLI: 2
            sites") 1 5 "GTGCAC" "CACGTG")                    
            #122=(CRSFolderPar                                                 
                  (CFolderPar                                                  
                   (CGroupPar (CParagraph 111381360 (8 0) 1 1 1 0 178)         
                             (CObjectList                                      
                              #123=(CGroupPar                                  
                                    (CParagraph 0 (10 #1# 0) 1 2 2 0 180)      
                                       (CObjectList                            
                                        #124=(CLinePar                         
                                              (CParagraph 0 (1 #1# 1) 1 2 2 0
            191)                                         " N1: 7236 " 1)       
                                                   #125=(CLinePar              
                                                         (CParagraph 0 (1 #1#
            2) 1 2 2 0 191)                                         " N2: 9391
            " 1))))) "AvaI: 2 sites")                         1 5 "CYCGRG"
            "GRGCYC")                                        #126=(CRSFolderPar
                                                                   (CFolderPar 
                                                                    (CGroupPar
            (CParagraph 111380976 (8 0) 1 1 1 0 178)                          
            (CObjectList                                                       
             #127=(CGroupPar                                                   
                   (CParagraph 0 (10 #2# 0) 1 2 2 0 180)                       
                      (CObjectList                                             
                       #128=(CLinePar                                          
                             (CParagraph 0 (1 #2# 1) 1 2 2 0 191)              
                                      " N1: 6507 " 1))))) "BamHI: 1 site")     
                               1 5 "GGATCC" "CCTAGG")                          
                         #129=(CRSFolderPar                                    
                               (CFolderPar                                     
                                (CGroupPar (CParagraph 111381008 (8 0) 1 1 1 0
            178)                           (CObjectList                        
                                            #130=(CGroupPar                    
                                                  (CParagraph 0 (10 #3# 0) 1 2
            2 0 180)                                  (CObjectList             
                                                       #131=(CLinePar          
                                                             (CParagraph 0 (1
            #3# 1) 1 2 2 0 191)                                         " N1:
            7434 " 1))))) "ClaI: 1 site")                         2 4 "ATCGAT"
            "TAGCTA")                                        #132=(CRSFolderPar
                                                                   (CFolderPar 
                                                                    (CGroupPar
            (CParagraph 111381136 (8 0) 1 1 1 0 178)                          
            (CObjectList                                                       
             #133=(CGroupPar                                                   
                   (CParagraph 0 (10 #4# 0) 1 2 2 0 180)                       
                      (CObjectList                                             
                       #134=(CLinePar                                          
                             (CParagraph 0 (1 #4# 1) 1 2 2 0 191)              
                                      " N1: 2627 " 1))))) "EcoRI: 1 site")     
                               1 5 "GAATTC" "CTTAAG")                          
                         #135=(CRSFolderPar                                    
                               (CFolderPar                                     
                                (CGroupPar (CParagraph 111381200 (8 0) 1 1 1 0
            178)                           (CObjectList                        
                                            #136=(CGroupPar                    
                                                  (CParagraph 0 (10 #5# 0) 1 2
            2 0 180)                                  (CObjectList             
                                                       #137=(CLinePar          
                                                             (CParagraph 0 (1
            #5# 1) 1 2 2 0 191)                                         " N1:
            7763 " 1)))))                                  "HindIII: 1 site") 1
            5 "AAGCTT" "TTCGAA")                    #138=(CRSFolderPar         
                                                          (CFolderPar          
                                                           (CGroupPar
            (CParagraph 111380912 (8 0) 1 1 1 0 178)                          
            (CObjectList                                                       
             #139=(CGroupPar                                                   
                   (CParagraph 0 (10 #6# 0) 1 2 2 0 180)                       
                      (CObjectList                                             
                       #140=(CLinePar                                          
                             (CParagraph 0 (1 #6# 1) 1 2 2 0 191)              
                                      " N1: 6403 " 1)                          
                                #141=(CLinePar                                 
                                      (CParagraph 0 (1 #6# 2) 1 2 2 0 191)     
                                               " N2: 6519 " 1)                 
                                         #142=(CLinePar                        
                                               (CParagraph 0 (1 #6# 3) 1 2 2 0
            191)                                         " N3: 8659 " 1)))))
            "NcoI: 3 sites")                         1 5 "CCATGG" "GGTACC")    
                                               #143=(CRSFolderPar              
                                                     (CFolderPar               
                                                      (CGroupPar (CParagraph
            111381552 (8 0) 1 1 1 0 178)                           (CObjectList
                                                                   
            #144=(CGroupPar                                                    
                  (CParagraph 0 (10 #7# 0) 1 2 2 0 180)                        
                     (CObjectList                                              
                      #145=(CLinePar                                           
                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191)               
                                     " N1: 3888 " 1)                           
                               #146=(CLinePar                                  
                                     (CParagraph 0 (1 #7# 2) 1 2 2 0 191)      
                                              " N2: 4571 " 1)                  
                                        #147=(CLinePar                         
                                              (CParagraph 0 (1 #7# 3) 1 2 2 0
            191)                                         " N3: 7083 " 1)       
                                                   #148=(CLinePar              
                                                         (CParagraph 0 (1 #7#
            4) 1 2 2 0 191)                                         " N4: 7392
            " 1)                                               #149=(CLinePar  
                                                                    
            (CParagraph 0 (1 #7# 5) 1 2 2 0 191)                               
                     " N5: 8557 " 1))))) "PstI: 5 sites")                      
              5 1 "CTGCAG" "GACGTC")                                       
            #150=(CRSFolderPar                                                 
                  (CFolderPar                                                  
                   (CGroupPar (CParagraph 111381168 (8 0) 1 1 1 0 178)         
                             (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG"      
                              "GGGCCC")                                        
                        #151=(CRSFolderPar                                     
                              (CFolderPar                                      
                               (CGroupPar (CParagraph 111381232 (8 0) 1 1 1 0
            178)                           (CObjectList)) "XmaI: 0 sites") 1 5
            "CCCGGG"                         "GGGCCC")))
            "Restriction/Methylation Map"))))    (CGraphView                   
                                                  (CWStyleSheet                
                                                   (CObjectList                
                                                    #152=(CWidgetStyle "RSite
            Label" 1 (LOGPEN 0 0 13408563) 1 0 1             (LOGFONT 0 0 0 0
            400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5             "@N
            (@S)" 0)                                                 
            #153=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1           
                  (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1
                        "@N" 0)                                                
                  #154=(CWidgetStyle "Molecule Label 2" 0 0 1                  
                        (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia")
            0.555556 0 1 16             "@L bp" 0)                             
                                  #155=(CWidgetStyle "Molecule Label 1" 0 0 1  
                                        (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34
            "Verdana") 0.833333 0 1 1             "@N" 0)                      
                                            #156=(CWidgetStyle "Shape 3" 1
            (LOGPEN 0 0 3355545) 1 1                   (LOGBRUSH 0 6724095 0) 0
            0 1 (LOGSHAPE 9 1 0.8 1.8 0))        #157=(CWidgetStyle "Shape 1" 1
            (LOGPEN 0 0 6723840) 1 1                   (LOGBRUSH 0 10079334 0)
            0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))       #158=(CWidgetStyle "Axis" 1
            (LOGPEN 0 0 10079436) 2 1                     (LOGBRUSH 0 13434879
            0) 0 0 1 (LOGSHAPE 10 1 0 0 0))          #159=(CWidgetStyle "Line
            2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1              (LOGSHAPE 1 1.9 0
            0 0))                                       #160=(CWidgetStyle
            "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1              (LOGSHAPE 1
            1.9 0 0 0))                                      
            #161=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0
            1             (LOGSHAPE 1 1.9 0 0 0))                              
                    #162=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1
            0 1             (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia")
            0.555556 128 1             5 "@N (@S)" 0)                          
                                 #163=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0)
            1 1                         (LOGBRUSH 0 16777215 0) 1              
                                        (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18
            "Times New Roman") 0.8 0             1 2 "?" 0)                    
                                           #164=(CWidgetStyle "Mark 1" 0 0 1   
                                                 (LOGFONT 0 0 0 0 400 0 0 0 2 7
            48 2 2 "Windings") 0.7 0 1 2 "?"             0)                    
                                                   #165=(CWidgetStyle "Motif
            Label" 1 (LOGPEN 0 0 16744512) 1 0 1             (LOGFONT 0 0 0 0
            400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608             1 65535
            "@N (@H)" 0)                                         
            #166=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1       
                  (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1
            48             "@F bp (molecule @L bp)" 0)                         
                     #167=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0
            1              (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91
            0 1 1             "Fragment of @N" 0)                              
                        #168=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1      
                              (LOGBRUSH 2 8388608 5) 0 0 0)                    
                        #169=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1
            (LOGBRUSH 0 128 0) 0             0 0)                              
                                       #170=(CWidgetStyle "Shape 0" 1 (LOGPEN 0
            0 0) 1 1 (LOGBRUSH 0 0 0) 0 0             0)                       
                                                #171=(CWidgetStyle "ORF" 1
            (LOGPEN 0 0 16384) 8 0 0 0 1                   (LOGSHAPE 7 0.2
            3.41182 2.86186 0.609808))                    #172=(CWidgetStyle
            "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0)         #173=(CWidgetStyle
            "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0)      
            #174=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0)     
             #175=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
                 #176=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0)        
                 #177=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0)       
                 #178=(CWidgetStyle "Void" 0 0 0 0 0)                          
                 #179=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1     
                       (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman")
            0.91 0             1 3 "@T @N " 0)                                 
                         #180=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0
            0)           #181=(CWidgetStyle "Annotation" 0 0 1                 
                               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New
            Roman") 0.91 0             0 0)                                    
                                 #182=(CWidgetStyle "Position Label" 1 (LOGPEN
            0 0 8388608) 1 0 1             (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1
            34 "Arial") 0.63 8388608 1 1             "@N" 0)                   
                                               #183=(CWidgetStyle "Range" 1
            (LOGPEN 0 0 0) 1 1                           (LOGBRUSH 0 16777215
            0) 0 0 0)                                #184=(CWidgetStyle "Range
            Label" 1 (LOGPEN 0 0 8388608) 1 0 1             (LOGFONT 0 0 0 0
            400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1             "@N" 0) 
                                                                
            #185=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1          
                  (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman")     
                   0.611111 0 1 65535 "@N" 0)                                  
             #186=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1       
                   (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255
            1 1             "@N" 0)                                            
                      #187=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1        
                            (LOGBRUSH 0 16777113 0) 1                          
                            (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9
            0 0 1             (LOGSHAPE 9 1 0.8 1.8 0))                        
                        #188=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH
            2 39423 3) 0 0             1 (LOGSHAPE 9 1 0.8 1.8 0))             
                                 #189=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0
            4227264) 1 0 1                 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1
            34 "Arial") 0.944444 8388608             1 1 "@N" 0)               
                                               #190=(CWidgetStyle "Label 3" 1
            (LOGPEN 0 0 8421376) 1 0 1                 (LOGFONT 0 0 0 0 700 255
            0 0 0 3 2 1 34 "Arial") 0.833333 255 1             5 "@N (@S)" 0)  
                                                         #191=(CWidgetStyle
            "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1                 (LOGFONT 0 0
            0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5             "@N
            (@S)" 0)) 0.164644 1.74233 0.164644 2.53336              (70
            (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67       
            (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1
            -365)     1 1 0 1 1                                                
                      (mapper: 26.7802 -21.4402 40.0378 40.0378 0.01 10 -1
            13245 13245 1 0 0)     #192=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0
            Nil 1701672291 100)           (CObjectList                         
                                           #193=(CGroupWidget (CWidget 1 (0 0)
            1 2 0 0 Nil -1435 100)                  (CObjectList               
                                                     #194=(CAxis               
                                                           (CWideLine          
                                                            (CWidget 0 (0 0) 1
            2 0 0 #158# 110910364 0)                          (LOGPEN 0 0
            10079436) 2 (LOGBRUSH 0 13434879 0) 1                         
            6.27466 6.27266 1 0.0214037) 0.0473627)                     
            #195=(CLabel                                                       
                  (CWidget 1001 (0 0) 1 2 0 0 #155# -591505799 100)            
                        (LOGPEN 0 0 0) 1                                       
                        (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana")     
                               1.74233 0.833333 0 "rag2-YEATS4/pISceI" "@N" 1 0
                                    0.871165 0 -10 11.1145 1.44863 Nil)        
                              #196=(CLabel                                     
                                    (CWidget 1002 (0 0) 1 2 0 0 #154#
            -576825511 100)                         (LOGPEN 0 0 0) 1           
                                                    (LOGFONT 38 14 0 0 400 0 0
            0 0 3 2 1 18 "Georgia")                         1.74233 0.555556 0
            "13245 bp" "@L bp" 16 0 -0.871165                         0 -10.8
            3.77144 0.924127 Nil)) (CObjectList))            #197=(CGroupWidget
            (CWidget 10 (6 0) 1 2 0 0 Nil -1365 100)                 
            (CObjectList                                                       
             #198=(CGroupWidget                                                
                   (CWidget 4 (7 4 0) 1 2 0 0 Nil -1086 100)                   
                   (CObjectList                                                
                    #199=(CWideArrow                                           
                          (CWideLine                                           
                           (CWidget 0 (3 #18# 0) 1 2 0 0 #156# 0 100)          
                                  (LOGPEN 0 0 3355545) 1                       
                                  (LOGBRUSH 0 6724095 0) 1 1.83246 2.17205 1   
                                         0.082322) 0.8 1.8 0)                  
                                  #200=(CLabel                                 
                                        (CWidget 0 (0 0) 1 2 0 0 #153# -69997
            100)                                (LOGPEN 0 0 0) 1               
                                                (LOGFONT 46 17 0 0 700 0 0 0 0
            3 2 1 34                                 "Arial") 1.74233 0.666667
            0 "EGFP [*]" "@N"                                1 -12.8287 5.22699
            0 -10 3.87134 1.07399                                #199#)        
                                                           #201=(CWideArrow    
                                                                 (CWideLine    
                                                                  (CWidget 0 (3
            #21# 0) 1 2 0 0 #156# 0 100)                                
            (LOGPEN 0 0 3355545) 1                                             
            (LOGBRUSH 0 6724095 0) 1 2.75367 3.18561 1                         
                   0.082322) 0.8 1.8 0)                                        
            #202=(CLabel (CWidget 0 (0 0) 1 2 0 0 #153# 0 100)                 
                          (LOGPEN 0 0 0) 1                                     
                          (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34              
                              "Arial") 1.74233 0.666667 0 "CDK4" "@N" 1        
                                   -4.21786 -15.681 0 -10 2.62252 1.07399
            #201#))                         (CObjectList))                     
                                      #203=(CGroupWidget                       
                                            (CWidget 21 (7 21 0) 1 2 0 0 Nil
            -667 100)                          (CObjectList                    
                                                #204=(CScratch                 
                                                      (CWidget 0 (3 #19# 0) 1 2
            0 0 #161# 703 100)                                (LOGPEN 0 8
            13395507) 10 1 2.08159 1.9                                0.082322
            1)                                                   #205=(CLabel  
                                                                       (CWidget
            0 (0 0) 1 2 0 0 #153# 257 100)                               
            (LOGPEN 0 0 0) 1                                                   
            (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34                            
                "Arial") 1.74233 0.666667 0                                    
               "EGFP-like chromophore" "@N" 1 -16.3986                         
                  3.48466 0 -10 11.1894 1.07399 #204#)                         
            #206=(CScratch                                                     
                  (CWidget 0 (3 #20# 0) 1 2 0 0 #161# 714 100)                 
                          (LOGPEN 0 8 13395507) 10 1 2.16495 1.9               
                            0.082322 1)                                        
                      #207=(CLabel (CWidget 0 (0 0) 1 2 0 0 #153# 37 100)      
                                     (LOGPEN 0 0 0) 1                          
                                     (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34   
                                         "Arial") 1.74233 0.666667 0           
                                        "mutated BseRI restriction site" "@N" 1
                                           -17.8496 1.74233 0 -10 13.9618
            1.07399 #206#))                         (CObjectList))             
                                              #208=(CGroupWidget               
                                                    (CWidget 22 (7 22 0) 1 2 0
            0 Nil -877 100)                          (CObjectList              
                                                      #209=(CWideArrow         
                                                            (CWideLine         
                                                             (CWidget 0 (3 #13#
            0) 1 2 0 0 #169# 0 100)                                 (LOGPEN 0 0
            0) 1 (LOGBRUSH 0 128 0) 1                                 0.766802
            1.17459 1 0.082322) 0.8 1.8 0)                         
            #210=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #153# -588884431 100)               
                            (LOGPEN 0 0 0) 1                                   
                            (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34            
                                "Arial") 1.74233 0.666667 0 "Ampicillin-R"     
                                      "@N" 1 -13.2826 13.9386 0 -10 5.54476
            1.07399                                #209#)) (CObjectList))      
                                      #211=(CGroupWidget                       
                                            (CWidget 25 (7 25 0) 1 2 0 0 Nil
            -1295 100)                         (CObjectList                    
                                                #212=(CLine                    
                                                      (CWidget 0 (3 #15# 0) 1 2
            0 0 #172# 541676622                                 100) (LOGPEN 0
            6 32768) 8 0.835356 2.68546                                2.74751)
                                                                 #213=(CLabel  
                                                                       (CWidget
            0 (0 0) 1 2 0 0 #153# -67108864 100)                               
            (LOGPEN 0 0 0) 1                                                   
            (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34                            
                "Arial") 1.74233 0.666667 0 "SV40 pA" "@N" 1                   
                        -11.826 -6.96932 0 -10 3.82139 1.07399 #212#)          
                           #214=(CLine                                         
                                 (CWidget 0 (3 #16# 0) 1 2 0 0 #172#           
                                  1819632485 100) (LOGPEN 0 6 32768) 8         
                                  0.835356 1.62454 1.71832)                    
                            #215=(CLabel (CWidget 0 (0 0) 1 2 0 0 #153# 0 100) 
                                          (LOGPEN 0 0 0) 1                     
                                          (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1
            34                                 "Arial") 1.74233 0.666667 0     
                                              "SV40 late polyA (from pCS2+)"
            "@N" 1                                -18.3583 8.71165 0 -10
            13.3624 1.07399 #214#))                         (CObjectList))     
                                                      #216=(CGroupWidget       
                                                            (CWidget 29 (7 29
            0) 1 2 0 0 Nil 1919885412 100)                         (CObjectList
                                                                   
            #217=(CWideArrow                                                   
                  (CWideLine                                                   
                   (CWidget 0 (3 #10# 0) 1 2 0 0 #157# 0 100)                  
                          (LOGPEN 0 0 6723840) 1                               
                          (LOGBRUSH 0 10079334 0) 1 3.19224 6.27319 1          
                                  0.082322) 0.8 1.8 0)                         
                           #218=(CLabel                                        
                                 (CWidget 0 (0 0) 1 2 0 0 #153# 100 100)       
                                    (LOGPEN 0 0 0) 1                           
                                    (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34    
                                        "Arial") 1.74233 0.666667 0 "zrag2
            promoter"                                "@N" 1 15.0858 0 0 -10
            7.1932 1.07399 #217#))                         (CObjectList))      
                                                     #219=(CGroupWidget        
                                                           (CWidget 30 (7 30 0)
            1 2 0 0 Nil -946 100)                          (CObjectList        
                                                            #220=(CWideArrow   
                                                                  (CWideLine   
                                                                   (CWidget 0
            (3 #17# 0) 1 2 0 0 #169# 0 100)                                
            (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356                       
                     2.17205 2.59784 1 0.082322) 0.8 1.8 0)                    
                 #221=(CLabel                                                  
                       (CWidget 0 (0 0) 1 2 0 0 #153# -598059107 100)          
                                 (LOGPEN 0 0 0) 1                              
                                 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34       
                                     "Arial") 1.74233 0.666667 0 "cmlc2
            promoter"                                "@N" 1 -14.7783 -3.48466 0
            -10 7.34306                                1.07399 #220#))
            (CObjectList))                         #222=(CGroupWidget          
                                                         (CWidget 33 (7 33 0) 1
            2 0 0 Nil -1225 100)                         (CObjectList          
                                                          #223=(CWideArrow     
                                                                (CWideLine     
                                                                 (CWidget 0 (3
            #14# 0) 1 2 0 0 #156# 0 100)                                
            (LOGPEN 0 0 3355545) 1                                             
            (LOGBRUSH 0 6724095 0) 1 0.379375 0.698599 1                       
                     0.082322) 0.8 1.8 1)                                      
              #224=(CLabel                                                     
                    (CWidget 0 (0 0) 1 2 0 0 #153# 2082602612 100)             
                              (LOGPEN 0 0 0) 1                                 
                              (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34          
                                  "Arial") 1.74233 0.666667 0 "Ori" "@N" 1     
                                      -7.78406 15.681 0 -10 1.49858 1.07399
            #223#))                         (CObjectList))                     
                                      #225=(CGroupWidget                       
                                            (CWidget 87 (7 87 0) 1 2 0 0 Nil
            -946 100)                          (CObjectList                    
                                                #226=(CScratch                 
                                                      (CWidget 0 (3 #11# 0) 1 2
            0 0 #161# 843 100)                                (LOGPEN 0 8
            13395507) 10 1 1.56297 1.9                                0.082322
            1)                                                   #227=(CLabel  
                                                                       (CWidget
            0 (0 0) 1 2 0 0 #153# 14553028 100)                               
            (LOGPEN 0 0 0) 1                                                   
            (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34                            
                "Arial") 1.74233 0.666667 0 "I-SceI (Rev)"                     
                      "@N" 1 -14.9326 10.454 0 -10 5.46983 1.07399             
                              #226#)                                           
                        #228=(CScratch                                         
                              (CWidget 0 (3 #12# 0) 1 2 0 0 #161# 541676622    
                                        100) (LOGPEN 0 8 13395507) 10 1
            0.183294 1.9                                0.082322 1)            
                                                  #229=(CLabel                 
                                                        (CWidget 0 (0 0) 1 2 0
            0 #153# -68656 100)                                (LOGPEN 0 0 0) 1
                                                               (LOGFONT 46 17 0
            0 700 0 0 0 0 3 2 1 34                                 "Arial")
            1.74233 0.666667 0 "I-SceI (Rev)"                               
            "@N" 1 -5.60745 19.1656 0 -10 5.46983 1.07399                      
                     #228#)) (CObjectList))) (CObjectList))           
            #230=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1295 100)        
                     (CObjectList                                              
                      #231=(CGroupWidget                                       
                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -461 100)        
                            (CObjectList                                       
                             #232=(CScratch                                    
                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #160# 111008752
                                            100) (LOGPEN 0 6 10053171) 8 1
            3.19177 1.9                                0.082322 1)             
                                                 #233=(CLabel (CWidget 0 (0 0)
            1 2 0 0 #162# 0 100)                                (LOGPEN 0 0
            153) 1                                                  (LOGFONT 38
            14 0 0 400 0 0 0 0 3 2 1 18                                
            "Georgia") 1.74233 0.555556 128                                   
            "{\\i Bam}HI (6507)" "@N (@S)" 5 4.18047                           
                -12.1963 0 -10 5.66964 0.924127 #232#))                        
            (CObjectList))                                               
            #234=(CGroupWidget                                                 
                  (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -810 100)                  
                  (CObjectList                                                 
                   #235=(CScratch                                              
                         (CWidget 1 (1 #3# 1) 1 2 0 0 #160# 111006064          
                                  100) (LOGPEN 0 6 10053171) 8 1 2.75272 1.9   
                                        0.082322 1)                            
                                  #236=(CLabel (CWidget 0 (0 0) 1 2 0 0 #162# 0
            100)                                (LOGPEN 0 0 153) 1             
                                                (LOGFONT 38 14 0 0 400 0 0 0 0
            3 2 1 18                                 "Georgia") 1.74233
            0.555556 128                                    "{\\i Cla}I (7434)"
            "@N (@S)" 5 -10.8884                                -8.71165 0 -10
            4.47078 0.924127 #235#))                         (CObjectList))    
                                                       #237=(CGroupWidget      
                                                             (CWidget 1 (10 #4#
            0) 1 2 0 0 Nil -179 100)                         (CObjectList      
                                                              #238=(CScratch   
                                                                    (CWidget 1
            (1 #4# 1) 1 2 0 0 #160# 112432464                                
            100) (LOGPEN 0 6 10053171) 8 1 5.02944 1.9                         
                  0.082322 1)                                                  
            #239=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #162# 7862276 100)                  
                         (LOGPEN 0 0 153) 1                                    
                         (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18               
                             "Georgia") 1.74233 0.555556 128                   
                            "{\\i Eco}RI (2627)" "@N (@S)" 5 14.1075           
                                3.48466 0 -10 5.19509 0.924127 #238#))         
                           (CObjectList))                                      
                     #240=(CGroupWidget                                        
                           (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -1086 100)        
                            (CObjectList                                       
                             #241=(CScratch                                    
                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #160# 112432504
                                            100) (LOGPEN 0 6 10053171) 8 1
            2.5969 1.9                                0.082322 1)              
                                                #242=(CLabel (CWidget 0 (0 0) 1
            2 0 0 #162# 0 100)                                (LOGPEN 0 0 153)
            1                                                  (LOGFONT 38 14 0
            0 400 0 0 0 0 3 2 1 18                                 "Georgia")
            1.74233 0.555556 128                                    "{\\i
            Hin}dIII (7763)" "@N (@S)" 5 -13.7794                              
             -5.22699 0 -10 5.59471 0.924127 #241#))                        
            (CObjectList))                                               
            #243=(CGroupWidget                                                 
                  (CWidget 2 (10 #0# 0) 1 2 0 0 Nil -252 100)                  
                  (CObjectList                                                 
                   #244=(CScratch                                              
                         (CWidget 1 (1 #0# 1) 1 2 0 0 #160# 112430944          
                                  100) (LOGPEN 0 6 10053171) 8 1 1.11965 1.9   
                                        0.082322 1)                            
                                  #245=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0
            100)                                (LOGPEN 0 0 13408563) 1        
                                                (LOGFONT 38 14 0 0 400 0 0 0 0
            3 2 1 18                                 "Georgia") 1.74233
            0.555556 0                                      "{\\i Apa}LI
            (10882)" "@N (@S)" 5 -14.3748                               
            12.1963 0 -10 5.71959 0.924127 #244#)                         
            #246=(CScratch                                                     
                  (CWidget 2 (1 #0# 2) 1 2 0 0 #160# 112638144                 
                           100) (LOGPEN 0 6 10053171) 8 1 0.529515 1.9         
                                  0.082322 1)                                  
                            #247=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0 100) 
                                          (LOGPEN 0 0 13408563) 1              
                                          (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1
            18                                 "Georgia") 1.74233 0.555556 0   
                                              "{\\i Apa}LI (12128)" "@N (@S)" 5
            -9.83134                                17.4233 0 -10 5.66964
            0.924127 #246#))                         (CObjectList))            
                                               #248=(CGroupWidget              
                                                     (CWidget 2 (10 #1# 0) 1 2
            0 0 Nil 710 100)                          (CObjectList             
                                                       #249=(CScratch          
                                                             (CWidget 1 (1 #1#
            1) 1 2 0 0 #160# 111008808                                 100)
            (LOGPEN 0 6 10053171) 8 1 2.8465 1.9                               
            0.082322 1)                                                  
            #250=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0 100)                 
                          (LOGPEN 0 0 13408563) 1                              
                          (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18              
                              "Georgia") 1.74233 0.555556 0                    
                             "{\\i Ava}I (7236)" "@N (@S)" 5 -6.54875          
                                 -12.1963 0 -10 4.79547 0.924127 #249#)        
                             #251=(CScratch                                    
                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #160# 65983504 
                                           100) (LOGPEN 0 6 10053171) 8 1
            1.82583 1.9                                0.082322 1)             
                                                 #252=(CLabel (CWidget 0 (0 0)
            1 2 0 0 #152# 0 100)                                (LOGPEN 0 0
            13408563) 1                                             (LOGFONT 38
            14 0 0 400 0 0 0 0 3 2 1 18                                
            "Georgia") 1.74233 0.555556 0                                     
            "{\\i Ava}I (9391)" "@N (@S)" 5 -14.1981                           
                6.96932 0 -10 4.74552 0.924127 #251#))                        
            (CObjectList))                                               
            #253=(CGroupWidget                                                 
                  (CWidget 3 (10 #6# 0) 1 2 0 0 Nil -670 100)                  
                  (CObjectList                                                 
                   #254=(CScratch                                              
                         (CWidget 1 (1 #6# 1) 1 2 0 0 #160# 0 100)             
                              (LOGPEN 0 6 10053171) 8 1 3.24103 1.9            
                               0.082322 1)                                     
                         #255=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0 100)    
                                       (LOGPEN 0 0 13408563) 1                 
                                       (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
                                           "Georgia") 1.74233 0.555556 0       
                                          "{\\i Nco}I (6403)" "@N (@S)" 5
            8.81694                                -10.454 0 -10 4.79547
            0.924127 #254#)                          #256=(CScratch            
                                                           (CWidget 2 (1 #6# 2)
            1 2 0 0 #160# 111006456                                 100)
            (LOGPEN 0 6 10053171) 8 1 3.18609 1.9                              
             0.082322 1)                                                  
            #257=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0 100)                 
                          (LOGPEN 0 0 13408563) 1                              
                          (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18              
                              "Georgia") 1.74233 0.555556 0                    
                             "{\\i Nco}I (6519)" "@N (@S)" 5 3.68806           
                                -13.9386 0 -10 4.74552 0.924127 #256#)         
                            #258=(CScratch                                     
                                  (CWidget 3 (1 #6# 3) 1 2 0 0 #160# 111005616 
                                           100) (LOGPEN 0 6 10053171) 8 1
            2.17253 1.9                                0.082322 1)             
                                                 #259=(CLabel (CWidget 0 (0 0)
            1 2 0 0 #152# 0 100)                                (LOGPEN 0 0
            13408563) 1                                             (LOGFONT 38
            14 0 0 400 0 0 0 0 3 2 1 18                                
            "Georgia") 1.74233 0.555556 0                                     
            "{\\i Nco}I (8659)" "@N (@S)" 5 -14.3105 0 0                       
                    -10 4.79547 0.924127 #258#)) (CObjectList))                
              #260=(CGroupWidget                                               
                    (CWidget 5 (10 #7# 0) 1 2 0 0 Nil -388 100)                
                    (CObjectList                                               
                     #261=(CScratch                                            
                           (CWidget 1 (1 #7# 1) 1 2 0 0 #160# 111006680        
                                    100) (LOGPEN 0 6 10053171) 8 1 4.4322 1.9  
                                         0.082322 1)                           
                                   #262=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152#
            0 100)                                (LOGPEN 0 0 13408563) 1      
                                                  (LOGFONT 38 14 0 0 400 0 0 0
            0 3 2 1 18                                 "Georgia") 1.74233
            0.555556 0                                      "{\\i Pst}I (3888)"
            "@N (@S)" 5 13.8276                                -3.48466 0 -10
            4.47078 0.924127 #261#)                          #263=(CScratch    
                                                                   (CWidget 2
            (1 #7# 2) 1 2 0 0 #160# 110841072                                
            100) (LOGPEN 0 6 10053171) 8 1 4.10871 1.9                         
                  0.082322 1)                                                  
            #264=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0 100)                 
                          (LOGPEN 0 0 13408563) 1                              
                          (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18              
                              "Georgia") 1.74233 0.555556 0                    
                             "{\\i Pst}I (4571)" "@N (@S)" 5 12.2859           
                                -6.96932 0 -10 4.42082 0.924127 #263#)         
                            #265=(CScratch                                     
                                  (CWidget 3 (1 #7# 3) 1 2 0 0 #160# 112431384 
                                           100) (LOGPEN 0 6 10053171) 8 1
            2.91896 1.9                                0.082322 1)             
                                                 #266=(CLabel (CWidget 0 (0 0)
            1 2 0 0 #152# 0 100)                                (LOGPEN 0 0
            13408563) 1                                             (LOGFONT 38
            14 0 0 400 0 0 0 0 3 2 1 18                                
            "Georgia") 1.74233 0.555556 0                                     
            "{\\i Pst}I (7083)" "@N (@S)" 5 -5.70377                           
                -13.9386 0 -10 4.47078 0.924127 #265#)                         
            #267=(CScratch                                                     
                  (CWidget 4 (1 #7# 4) 1 2 0 0 #160# 111007464                 
                           100) (LOGPEN 0 6 10053171) 8 1 2.77261 1.9          
                                 0.082322 1)                                   
                           #268=(CLabel                                        
                                 (CWidget 0 (0 0) 1 2 0 0 #152# 2081903016 100)
                                           (LOGPEN 0 0 13408563) 1             
                                           (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1
            18                                 "Georgia") 1.74233 0.555556 0   
                                              "{\\i Pst}I (7392)" "@N (@S)" 5
            -8.60786                                -10.454 0 -10 4.47078
            0.924127 #267#)                          #269=(CScratch            
                                                           (CWidget 5 (1 #7# 5)
            1 2 0 0 #160# 112432144                                 100)
            (LOGPEN 0 6 10053171) 8 1 2.22084 1.9                              
             0.082322 1)                                                  
            #270=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0 100)                 
                          (LOGPEN 0 0 13408563) 1                              
                          (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18              
                              "Georgia") 1.74233 0.555556 0                    
                             "{\\i Pst}I (8557)" "@N (@S)" 5 -14.0252          
                                 -1.74233 0 -10 4.47078 0.924127 #269#))       
                             (CObjectList))) (CObjectList))                    
                #271=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -598 100)    
                         (CObjectList) (CObjectList))                          
                   #272=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -842 100)  
                           (CObjectList) (CObjectList))) (CObjectList)))       
             (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0  
             (CStringList) 0 0 (CObList))).
FEATURES             Location/Qualifiers
     misc_feature    1..>6504
                     /vntifkey=29
                     /label="zrag2\promoter"
                     /note="Geneious type: promoter eukaryotic"
     CDS             6511..6957
                     /created_by="Raja"
                     /modified_by="Raja"
                     /label="LYZ"
     polyA_signal    6964..7094
                     /vntifkey=25
                     /label="SV40\pA"
     misc_feature    complement(7121..7318)
                     /created_by="Raja"
                     /label="SV40/Poly A tail"
     gene            complement(7557..8276)
                     /created_by="Raja"
                     /modified_by="Raja"
                     /label="EGFP"
     promoter        complement(8277..9175)
                     /created_by="Raja"
                     /modified_by="Raja"
                     /label="Cmlc2 promoter"
     misc_feature    9346..9348
                     /created_by="Raja"
                     /label="End Of Cmlc2 EGFP insert"
     misc_signal     complement(9462..9479)
                     /vntifkey=87
                     /label="I-SceI\(Rev)"
     misc._marker    10282..11142
                     /vntifkey=22
                     /label="Ampicillin-R"
     rep_origin      complement(11287..11960)
                     /vntifkey=33
                     /label="Ori"
     misc_signal     complement(12375..12392)
                     /vntifkey=87
                     /label="I-SceI\(Rev)"
ORIGIN      
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct
      901 aatgccccag tataaaccag caggtcaacc tgtagtctgt gtgggtccta atgccccagt
      961 ataaaccagc aggtctacct gtggtctgtg tgggtcctaa tgccccagta taaaccaaca
     1021 ggtcaacctg tgatctgtgt gggtcctaat gccccagtat aaaccagcag gtcaacctgt
     1081 ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg
     1141 gtcctaatgc cccagtataa accagcaggt caacctgtgg tctgtgtggg tcctaatgcc
     1201 ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc cagtataaac
     1261 cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa
     1321 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg
     1381 tgtgggtcct aatgccccag tataaaccag caggtcaacc tgtggtctgt gtgggtccta
     1441 atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta
     1501 taaaccagca ggtcaacctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag
     1561 gtctacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg
     1621 gtctgtgtgg gtcctaatgc cccagtataa accagcaggt caacctgtgg tctgtgtggg
     1681 tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc
     1741 cagtataaac cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc
     1801 agcaggtcaa cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac
     1861 ctgtggtctg tgtgggtcct aatgccccag tataaaccag caggtcaacc tgtggtctgt
     1921 gtgggtccta atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa
     1981 tgccccagta taaaccagca gatcaacctg tggtctgtgt gggtcctaat gccccagtat
     2041 aaaccagcag atcaacctgt ggtctgtgtg ggtcctaaag tcccaggata aaccagcatg
     2101 tcaacctgtg gtctgtgtgg gtcctaatgc cccggtataa accagcagat caacctgtgg
     2161 tctgtgtggg tcctaatgcc ccaggataaa ccagcaggtc aacctgtggt ctgtgtgggt
     2221 cctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc
     2281 agtatattga tggagactct atactgctcg gtgagctcca tctttcggat aagatgttaa
     2341 aacaaggtcc cgactctctg tggtggtaaa aaattcccag gatgtccttc ctaccctggc
     2401 atcctggaca aatttgctca ctgtcctctg tccatcatgg cctcctaacc atccccatat
     2461 cataattggc tttatcactc tagctggggc cgaaatcgca tacttccatt ttatgaggta
     2521 cgctaaaatc agtatgcgag ccaagtaata tgtccgaata cttccggcga gattctagag
     2581 tgtgcattcg atgcacgcta cgctattcca tgatgccccg tgagagaatt cttgaatcgg
     2641 agagaagcaa cgcaactcat gcgggtaggt tacgtgacga tgacatattg gcggatgtag
     2701 tacgtccaag ttccattcat actgctcaca ttcatatttt atagaatgtt cttttcccac
     2761 agccaagtag taagtttaaa ttcaaataca gtacctacta agtagtagta gacggtttcg
     2821 gacgcagccc ctgtctcctc tccaccaata tactggtgtg tagtgtgcgg tctggtgcaa
     2881 tatggctgcc atcgcgtcat ccaggtggat gctgcacact ggtggttgat gaggagattt
     2941 tgcccaaaaa agtgttttga gtttccagaa aagcgcgata taaatgtaag gaattatcat
     3001 tagcttatta taatggaact ttctggataa cttgtgccag agctaggggc cattaaaact
     3061 ttgccctggg gtacctcagg gagctgcaag gaagtgctat agggctaatg aaatgtttgg
     3121 gggaaaaaaa aaaattttat aagtaaaaat aaaactaaaa taattaataa tttcattgtt
     3181 gaaaattata gtaaaattga taaacaagat aagcagcatt agtataaaaa actaaaaaca
     3241 aacgtaattt aaaataatta aattgtttta aataaattat acaaaataat gtttacaacg
     3301 acacggtggt gcagtaggta gtgctgtcgc cagcaaagca agaaggtcgc tggttcgagc
     3361 ctcagctgga tcacttgatg tttctgtgtg gagcattcgc gtgcgtttaa ccccagatta
     3421 ataaagagac taagccgaaa agaaaataaa tgtatgaaaa tgtttacgta aaatgtaaat
     3481 aaatgcatga ttaataaaat atatgaattt taaagattga ggtagaattg ttttattatt
     3541 cacacatttg gttattttta atcagtgaca acttgtgatg ctctttatat tgtttattac
     3601 gctactttga atattcagtc tcaaattgca tgtaaatacg acttcaaaac agaattagtt
     3661 taatttaatt gcctgtaaac agtgttgtac tttgatggtg attgtctgat aataatatga
     3721 tttcactatt taaaatttga aaaaaaaaaa tctgaaaaat actcaccggc cactttatta
     3781 ggtacacctg tctaactgct tgttaacgta aatttctaat cagccaatca ccaggcagca
     3841 actcagtgca tttaggcatg tagacatggt caagacgatc tgctgcagtt caaagcgagc
     3901 atcagaatgg agaagcaagg tgatttaagt gactttaaac gtgacgtgtt tgttggtgcc
     3961 agacgggctg gtctgagtat ttcagaaact gctgatttac tggggttttc acacacaaac
     4021 atctctgggg tttacagaga atggtccaaa aaaagataat atccagtgag cggcagttct
     4081 gtgggggcaa atgtcttgtt gatgccagag gtcagaggag aatggcctga ctggttccag
     4141 ctgatagaaa ggcaacagta attcaaataa ccactcgtta gaactgaggc atgcagaaga
     4201 gcacctttga acacaacatg tcgaaccttg aggcgggtgg gctacagcag ctcaagacca
     4261 catcgggtgc cactcctgtc agctaagaac aggaaactga ggctacaatt cgcacaggct
     4321 caacataagt agacaataga agattgaaca ttatgttgcc tggtcagatg gatacattct
     4381 accttgtatc aacagttcag gctggtgatg gtggtgtaat ggtgtggggg atatattctt
     4441 ggcacacttt gggcccatta gtaccaatta accatcgtgt caacgccaca gcctaactga
     4501 gtattgttgc tgaccatgtt catttctttt tgaccacagt gtacccatct tctgatggct
     4561 acttcctgca ggataatgca ccatgtcata aagcgtgaat gatctcagac tggtttcttg
     4621 aacatgacaa tgagttcact gtgctcgaat gacctccaca gtcaccagat ctcaatccaa
     4681 tagagcgcct ttggggtgtg gtagaatgga agattcgcat cctggatgtg cagccgacaa
     4741 atctgcatga tgctagcatg tcaatatgga ccaaaatctc tgaggaattt tccagtacct
     4801 ggttgaaatg caaaaggaag ggaatctatg ctaaggcaaa aagaggtcca acccagtact
     4861 agtggggtgt acataataaa gtggccggtg agtgtatatt gttaaggata tctagataat
     4921 ccaaataatt tacagcacta tttaaaaaaa aaaactttat gtcaccaagt ctgcatttat
     4981 ttgatcacaa ttacagtcga aaaagcaata tcgggataca ttgtttccat ttaaaataac
     5041 tatattatat tttaaatatt tttgacaatg ttatttcttt aatgccaaag ctaaattgta
     5101 ttcaatctga attttagtgt cacatgatcc ttcagaaaat attaacacat tgctaaaaat
     5161 gtatattatt atcaatttgg attttttttt ttttttgtat attaacctat gctattggtt
     5221 actatacaaa agtattactg tgaatatatg catatacaga accgtatcaa gcgcgggggg
     5281 tggttgggtt atgtccccat ttggaaagac ctggaaacat tcataatgac taaaattgca
     5341 cggaaaaata taatatctat gagtattgct gatcagcagc acaaaaccag aactccatat
     5401 taataaacag ccaatgatgc agtgagtttg tgcatgtttt cattcaaact cgtgtgattg
     5461 tgctcaaaca cctgttgtga gcagcagttg caaacagtaa acctcaagag gagccagtca
     5521 accttcacaa acactcaacc tctggctcac tcactaaaca attcgcgtta atatctaaaa
     5581 taaaaatttc cagcaccaaa cagctctcag atttcggagt acactgacca agaggtaaga
     5641 agtaacgtta cattactagg acgactattg taagactaga tatgtaatat ttggaagcgt
     5701 acgtgtttta agtgacatat agagtagagg aactgatgga aatacgattt gcataatgta
     5761 aaatgatata tattaaaata taggctacag taggtaacgt ggtttacata taacgttact
     5821 gtgttaatgt agtgctcagt tactcgtgtt gacagttatt attagtgctt gctaatttgt
     5881 ttgttaatgc ttctcttgcg tatttgatta ccttcaggcg aatctaaaac atatcagatg
     5941 tagaaacggc agaaaaatgt tgaagaaaat acgagaatag tagcttttgg agcacataga
     6001 aaagtacatc agataaagcc taccgtttaa acgcagacgc cgctctcgca ctgaatgctg
     6061 ggtactgtag tccttatatt gtatgcttaa tgtgcgtctc aacgggaaac tagagttagg
     6121 aaaactacaa tttcctgtgg aaaatgtttc tgttttcggg gaaacagcga aagaaagata
     6181 tggttccgcg tttttttctt actttgaata ataaaataag acctaactac acacttcgct
     6241 tactcttatg ctaatttgaa gcactagcat tctttgatac aatgataatc atttaaacat
     6301 cacagttata ctacaaatac tatggttaaa ctatgtctag tgtagtgtaa cactatggtt
     6361 gccattgtta tctttaagtt taagtattat ctgtggtaaa gccatggttg gtttttataa
     6421 gaggtaataa aattaacatt gtacaattaa atgactataa attaatgttt ttttttattt
     6481 tttttacaca gctaccttca aaaaggatcc atgaaggctc tcattgttct ggggcttgtc
     6541 ctcctttctg ttacggtcca gggcaaggtc tttgaaaggt gtgagttggc cagaactctg
     6601 aaaagattgg gaatggatgg ctacagggga atcagcctag caaactggat gtgtttggcc
     6661 aaatgggaga gtggttacaa cacacgagct acaaactaca atgctggaga cagaagcact
     6721 gattatggga tatttcagat caatagccgc tactggtgta atgatggcaa aaccccagga
     6781 gcagttaatg cctgtcattt atcctgcagt gctttgctgc aagataacat cgctgatgct
     6841 gtagcttgtg caaagagggt tgtccgtgat ccacaaggca ttagagcatg ggtggcatgg
     6901 agaaatcgtt gtcaaaacag agatgtccgt cagtatgttc aaggttgtgg agtgtaaatc
     6961 gatcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     7021 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     7081 gcaataaaca agttaacaac aaccattcat tttattacta ctaggtctgt actattctat
     7141 gtaactactc aaacctgttt ggtgttgatc ttacgtcact ttttttacga aataaacact
     7201 ttaaacacta cgataacgaa ataaacattg gtaatattcg acgttatttg ttcaattgtt
     7261 gttgttaacg taagtaaaat acaaagtcca agtccccctc cacaccctcc aaaaaatttc
     7321 gttcattttg gagatgttta caccataccg actaatacta ggagatctag tctagagaac
     7381 aaataacgtc gaatattacc aatgtttatt tcgttatcgt agtgtttaaa gtgtttattt
     7441 cgtaaaaaaa gtgacgtaag atcaacacca aacaggtttg agtagttaca tagaatagta
     7501 cagacctaga tgcattatgc tgagtgatat caagatctcc gagctctccc cggcgaaatg
     7561 aacatgtcga gcaggtacgg ctctcactag ggccgccgcc agtgcttgag gtcgtcctgg
     7621 tacactagcg cgaagagcaa ccccagaaac gagtcccgcc tgacccacga gtccatcacc
     7681 aacagcccgt cgtcgtgccc cggcagcggc tacccccaca agacgaccat caccagccgc
     7741 tcgacgtgcg acggcaggag ctacaacacc gcctagaact tcaagtggaa ctacggcaag
     7801 aagacgaaca gccggtacta tatctgcaac accgacaaca tcaacatgag gtcgaacacg
     7861 gggtcctaca acggcaggag gaacttcagc tacgggaagt cgagctacgc caagtggtcc
     7921 cacagcggga gcttgaagtg gagccgcgcc cagaacatca acggcagcag gaacttcttc
     7981 taccacgcga ggacctgcat cggaagcccg taccgcctga acttcttcag cacgacgaag
     8041 tacaccagcc ccatcgccga cttcgtgacg tgcggcatcc agtcccacca gtgctcccac
     8101 ccggtcccgt gcccgtcgaa cggccaccac gtctacttga agtcccagtc gaacggcatc
     8161 caccgtagcg ggagcgggag cggcctgtgc gacttgaaca ccggcaaatg cagcggcagg
     8221 tcgagctggt cctacccgtg gtggggccac ttgtcgagaa gcgggaacga gtggtaccag
     8281 tgacagacga aacgacaacc agacccgagg acccagtgac cgaatgatta cctcagaaat
     8341 acatactcct gagaatagtt aacaagaaga tatttccaga cgtcacaaag acaagcaggg
     8401 gatgtacctg tgggtctcgg aggatttatg tcctcgggac tattgacgtg ttcacgagtc
     8461 taaggtcgtc ccacctttta ctctatttca cacgtctacc cctccccctg cacttactct
     8521 ctaaactccc tacttttcct acctacttgc gtaactttta tcggggaaag tgttttatta
     8581 tggtcattta acagtatatt aattactggt ctggaaatgg acatttacta cactacaagt
     8641 atatgtcttc ttacggttgt taaatgtcta ctaaaatgtt taagaattaa aaggaaaaga
     8701 tttacctaaa caagtgtgta atagagaagt ttcgttaact taaaagacct tttctaaccg
     8761 acacacacat tttccacgat gactccttaa attacactgt accttacttc gtttgtcgtc
     8821 ggattctgtc ctaccgtcct ttttacagtg tacaaatttt attctcactt tggttgctgg
     8881 acctgaatat ctctgacaca caggaatccg tttcgagaac atgtttgtca cgaacactga
     8941 cactttaagc cccaaacgga cctaacacaa tttacacacg aacctacata gtattttttc
     9001 ttagccaaga gacataaact gtaaaagaca ttaaatagat ttcaatgttg accgatacgg
     9061 actaaaaata gtgataactt agttagtaaa atttcttaca attcagacga ctaaacctct
     9121 cgagtaaatg taaataaaac ttacagagaa taaattgtgt tgactaaatt cgaaatctag
     9181 acgcttctat gccggtgccc acgagaacta ggacaccgac taaaacctga cacgacgagc
     9241 gtcgacgact acttagtgta tgaaggaggt aaaagaaggt gactaactga caatattaaa
     9301 gggattaaag gtccagttcc acgacacgta acaccattat ctacactggt ttcaggttca
     9361 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     9421 ttatgatcct ctagatcaga tccactagtt ctagagcggc cattaccctg ttatccctag
     9481 gccgccaccg cggtggagct ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     9541 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     9601 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     9661 ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta
     9721 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     9781 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     9841 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     9901 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     9961 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
    10021 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
    10081 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
    10141 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10201 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    10261 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    10321 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    10381 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    10441 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    10501 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    10561 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    10621 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10681 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10741 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10801 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10861 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10921 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10981 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    11041 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    11101 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    11161 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    11221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    11341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    11401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    11461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11701 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11761 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11821 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11881 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11941 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    12001 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    12061 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    12121 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    12181 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    12241 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    12301 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctgggtacc
    12361 gggccccccc tcgaattacc ctgttatccc tatcgagcaa aatcaatcct aatgtaaaat
    12421 agtatacgtt catcattata atgcaaacac tgtagaactg ttgtgttgtg ccttgcattt
    12481 cattctcagt ttcttatcgt ctgtctcttt ttacgacaga gctagaaaaa actagccttc
    12541 atttgccatc gccgtatgca aatccactcc agaactcaac aggtttgttc tgcggcgggt
    12601 gtaagtgtga acaccggcat gtaagtgcgc ttgagcagga gaataaagcg ctcagagaca
    12661 ccggaggaat cctttatttt gcgttgcgcc tcaggggatt cgctgagcct tctcttcatg
    12721 gccctgctct tcagacacag gtgctccgct gtttgtgttt t
//
