LOCUS       pdest_EGFP_Neo          8924 bp    DNA     circular UNA 14-JUN-2024
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV
            promoter, Neo.
ACCESSION   urn.local...2vb-hubtnw1
VERSION     urn.local...2vb-hubtnw1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        26..130
                     /gene="<html><body><i>bla</i></body></html>"
                     /label="AmpR promoter"
     CDS             join(131..199,200..991)
                     /transl_table=1
                     /product="<html><body>β-lactamase</body></html>"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVG
                     YIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLV
                     EYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGP
                     LLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQI
                     AEIGASLIKHW*"
                     /gene="<html><body><i>bla</i></body></html>"
                     /note="<html><body>confers resistance to ampicillin,
                     carbenicillin, and related antibiotics</body></html>"
                     /label="AmpR"
     primer_bind     complement(349..368)
                     /Tm=58
                     /Description="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     rep_origin      1162..1750
                     /direction="RIGHT"
                     /note="<html><body>high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication</body></html>"
                     /label="ori"
     primer_bind     1651..1670
                     /Tm=56
                     /Description="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     1904..1921
                     /Tm=58
                     /Description="L4440 vector, forward primer"
                     /label="L4440"
     protein_bind    2038..2059
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
                     /label="CAP binding site"
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /note="<html><body>promoter for the <i>E. coli lac</i>
                     operon</body></html>"
                     /label="lac promoter"
     protein_bind    2112..2128
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by
                     <i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the
                     <i>lac</i> operator to inhibit transcription in <i>E.
                     coli</i>. This inhibition can be relieved by adding
                     lactose or isopropyl-β-D-thiogalactopyranoside
                     (IPTG).</body></html>"
                     /label="lac operator"
     primer_bind     2117..2139
                     /Tm=58
                     /Description="In lacZ gene"
                     /label="M13/pUC Reverse"
     primer_bind     2136..2152
                     /Tm=49
                     /Description="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     2136..2152
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /label="M13 rev"
     primer_bind     2171..2191
                     /Tm=52
                     /Description="T3 promoter, forward primer"
                     /label="T3"
     promoter        2173..2191
                     /note="<html><body>promoter for bacteriophage T3 RNA
                     polymerase</body></html>"
                     /label="T3 promoter"
     promoter        2219..2445
                     /note="<html><body>Rous sarcoma virus
                     enhancer/promoter</body></html>"
                     /label="RSV promoter"
     LTR             2446..2626
                     /note="<html><body>truncated 5' long terminal repeat (LTR)
                     from HIV-1</body></html>"
                     /label="5' LTR (truncated)"
     misc_feature    2673..2798
                     /note="<html><body>packaging signal of human
                     immunodeficiency virus type 1</body></html>"
                     /label="HIV-1 Ψ"
     misc_feature    3291..3524
                     /note="<html><body>The Rev response element (RRE) of HIV-1
                     allows for Rev-dependent mRNA export from the nucleus to
                     the cytoplasm.</body></html>"
                     /label="RRE"
     misc_feature    4020..4136
                     /note="<html><body>central polypurine tract and central
                     termination sequence of HIV-1</body></html>"
                     /label="cPPT/CTS"
     enhancer        4159..4462
                     /note="<html><body>human cytomegalovirus immediate early
                     enhancer</body></html>"
                     /label="CMV enhancer"
     promoter        4463..4666
                     /note="<html><body>human cytomegalovirus (CMV) immediate
                     early promoter</body></html>"
                     /label="CMV promoter"
     primer_bind     4617..4637
                     /Tm=65
                     /Description="Human CMV immediate early promoter, forward
                     primer"
                     /label="CMV-F"
     primer_bind     4663..4687
                     /Tm=60
                     /Description="Human CMV promoter, forward primer"
                     /label="LNCX"
     misc_feature    4771..4824
                     /created_by="CH197744"
                     /label="attR1"
     gene            4825..5544
                     /created_by="CH197744"
                     /modified_by="CH197744"
                     /label="EGFP"
     misc_feature    5545..5592
                     /created_by="CH197744"
                     /label="attR2"
     misc_feature    5623..6211
                     /note="<html><body>woodchuck hepatitis
                     virus&nbsp;posttranscriptional regulatory
                     element</body></html>"
                     /label="WPRE"
     primer_bind     complement(5676..5696)
                     /Tm=55
                     /Description="WPRE, reverse primer"
                     /label="WPRE-R"
     CDS             complement(6094..6105)
                     /transl_table=1
                     /product="<html><body>Factor Xa recognition and cleavage
                     site</body></html>"
                     /codon_start=1
                     /translation="IEGR"
                     /label="Factor Xa site"
     promoter        6254..6753
                     /note="<html><body>mouse phosphoglycerate kinase 1
                     promoter</body></html>"
                     /label="PGK promoter"
     primer_bind     complement(6289..6312)
                     /Tm=69
                     /Description="Murine stem cell virus, reverse primer"
                     /label="MSCV-rev"
     primer_bind     6686..6705
                     /Tm=55
                     /Description="Mouse PGK promoter, forward primer"
                     /label="mPGK-F"
     CDS             6767..7570
                     /transl_table=1
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /codon_start=1
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /gene="<html><body><i>aph(3')-II</i> (or
                     <i>nptII</i>)</body></html>"
                     /note="<html><body>confers resistance to neomycin,
                     kanamycin, and G418 (Geneticin®)</body></html>"
                     /label="NeoR/KanR"
     primer_bind     complement(6830..6849)
                     /Tm=57
                     /Description="Neomycin resistance gene, reverse primer"
                     /label="Neo-R"
     primer_bind     7440..7459
                     /Tm=57
                     /Description="Neomycin resistance gene, forward primer"
                     /label="Neo-F"
     LTR             7661..7894
                     /note="<html><body>self-inactivating&nbsp;3' long terminal
                     repeat (LTR) from HIV-1</body></html>"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7966..8087
                     /note="<html><body>SV40 polyadenylation
                     signal</body></html>"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8003..8022)
                     /Tm=51
                     /Description="SV40 polyA, reverse primer"
                     /label="SV40pA-R"
     primer_bind     8057..8076
                     /Tm=54
                     /Description="SV40 polyA terminator, reverse primer"
                     /label="EBV-rev"
     rep_origin      8127..8262
                     /note="<html><body>SV40 origin of
                     replication</body></html>"
                     /label="SV40 ori"
     primer_bind     8189..8208
                     /Tm=56
                     /Description="SV40 promoter/origin, forward primer"
                     /label="SV40pro-F"
     primer_bind     complement(8282..8301)
                     /Tm=49
                     /Description="T7 promoter, forward primer"
                     /label="T7"
     promoter        complement(8283..8301)
                     /note="<html><body>promoter for bacteriophage T7 RNA
                     polymerase</body></html>"
                     /label="T7 promoter"
     primer_bind     complement(8311..8328)
                     /Tm=56
                     /Description="In lacZ gene. Also called M13-F20 or M13
                     (-21) Forward"
                     /label="M13 Forward"
     primer_bind     complement(8311..8327)
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /label="M13 fwd"
     primer_bind     complement(8320..8342)
                     /Tm=61
                     /Description="In lacZ gene"
                     /label="M13/pUC Forward"
     rep_origin      8469..8924
                     /direction="RIGHT"
                     /note="<html><body>f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand
                     synthesis</body></html>"
                     /label="f1 ori"
     primer_bind     complement(8556..8575)
                     /Tm=57
                     /Description="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     8766..8787
                     /Tm=57
                     /Description="F1 origin, forward primer"
                     /label="F1ori-F"
ORIGIN      
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4201 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4261 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4321 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4381 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4441 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     4501 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4561 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     4621 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     4681 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     4741 gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc ggggacaagt
     4801 ttgtacaaaa aagcaggctt caccatggtg agcaagggcg aggagctgtt caccggggtg
     4861 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4921 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4981 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     5041 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     5101 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     5161 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5221 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     5281 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     5341 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5401 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5461 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5521 ggcatggacg agctgtacaa gtaaaaccca gctttcttgt acaaagtggt cagctttctt
     5581 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac
     5641 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5701 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5761 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5821 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5881 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5941 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6001 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     6061 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6121 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6181 agtcggatct ccctttgggc cgcctccccg cctggaattc tgcagatatc cggactagtg
     6241 atctaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     6301 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     6361 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     6421 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     6481 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     6541 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     6601 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     6661 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     6721 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gccaatatgg gatcggccat
     6781 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6841 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6901 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6961 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7021 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7081 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7141 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7201 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7261 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     7321 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7381 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7441 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7501 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7561 gttcttctga ggggatccgt cgagggcccg gtacctttaa gaccaatgac ttacaaggca
     7621 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     7681 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7741 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7801 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7861 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7921 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7981 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8041 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     8101 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8161 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8221 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     8281 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     8341 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     8401 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     8461 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8521 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     8581 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8641 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     8701 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8761 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     8821 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     8881 aaaatttaac gcgaatttta acaaaatatt aacgcttaca attt
//
