LOCUS       pLenti_NFLAG_METTL1-WT_puro        8881 bp    DNA     circular UNA 14-JUN-2024
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV
            promoter, Puro.
ACCESSION   urn.local...5q0-hubwtm3
VERSION     urn.local...5q0-hubwtm3
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     95..112
                     /Tm=58
                     /Description="L4440 vector, forward primer"
                     /label="L4440"
     protein_bind    229..250
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /label="CAP binding site"
     promoter        join(265..270,271..288,289..295)
                     /note="<html><body>promoter for the <i>E. coli lac</i>
                     operon</body></html>"
                     /label="lac promoter"
     protein_bind    303..319
                     /note="<html><body>The <i>lac</i> repressor binds to the
                     <i>lac</i> operator to inhibit transcription in <i>E.
                     coli</i>. This inhibition can be relieved by adding
                     lactose or isopropyl-β-D-thiogalactopyranoside
                     (IPTG).</body></html>"
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by
                     <i>lacI</i></body></html>"
                     /label="lac operator"
     primer_bind     308..330
                     /Tm=58
                     /Description="In lacZ gene"
                     /label="M13/pUC Reverse"
     primer_bind     327..343
                     /Tm=49
                     /Description="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     327..343
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /label="M13 rev"
     primer_bind     362..382
                     /Tm=52
                     /Description="T3 promoter, forward primer"
                     /label="T3"
     promoter        364..382
                     /note="<html><body>promoter for bacteriophage T3 RNA
                     polymerase</body></html>"
                     /label="T3 promoter"
     promoter        410..636
                     /note="<html><body>Rous sarcoma virus
                     enhancer/promoter</body></html>"
                     /label="RSV promoter"
     LTR             637..817
                     /note="<html><body>truncated 5' long terminal repeat (LTR)
                     from HIV-1</body></html>"
                     /label="5' LTR (truncated)"
     misc_feature    864..989
                     /note="<html><body>packaging signal of human
                     immunodeficiency virus type 1</body></html>"
                     /label="HIV-1 Ψ"
     misc_feature    1482..1715
                     /note="<html><body>The Rev response element (RRE) of HIV-1
                     allows for Rev-dependent mRNA export from the nucleus to
                     the cytoplasm.</body></html>"
                     /label="RRE"
     misc_feature    2211..2327
                     /note="<html><body>central polypurine tract and central
                     termination sequence of HIV-1</body></html>"
                     /label="cPPT/CTS"
     enhancer        2350..2653
                     /note="<html><body>human cytomegalovirus immediate early
                     enhancer</body></html>"
                     /label="CMV enhancer"
     promoter        2654..2857
                     /note="<html><body>human cytomegalovirus (CMV) immediate
                     early promoter</body></html>"
                     /label="CMV promoter"
     primer_bind     2808..2828
                     /Tm=65
                     /Description="Human CMV immediate early promoter, forward
                     primer"
                     /label="CMV-F"
     primer_bind     2854..2878
                     /Tm=60
                     /Description="Human CMV promoter, forward primer"
                     /label="LNCX"
     misc_feature    2962..3015
                     /created_by="CH197744"
                     /label="attR1"
     gene            3016..3869
                     /created_by="CH197744"
                     /label="NFLAG METTL1-WT"
     misc_feature    complement(3872..3919)
                     /created_by="CH197744"
                     /modified_by="CH197744"
                     /label="attR2"
     misc_feature    3950..4538
                     /note="<html><body>woodchuck hepatitis
                     virus&nbsp;posttranscriptional regulatory
                     element</body></html>"
                     /label="WPRE"
     primer_bind     complement(4003..4023)
                     /Tm=55
                     /Description="WPRE, reverse primer"
                     /label="WPRE-R"
     CDS             complement(4421..4432)
                     /product="<html><body>Factor Xa recognition and cleavage
                     site</body></html>"
                     /codon_start=1
                     /translation="IEGR"
                     /transl_table=1
                     /label="Factor Xa site"
     promoter        4552..5051
                     /note="<html><body>mouse phosphoglycerate kinase 1
                     promoter</body></html>"
                     /label="PGK promoter"
     primer_bind     complement(4587..4610)
                     /Tm=69
                     /Description="Murine stem cell virus, reverse primer"
                     /label="MSCV-rev"
     primer_bind     4984..5003
                     /Tm=55
                     /Description="Mouse PGK promoter, forward primer"
                     /label="mPGK-F"
     CDS             5072..5671
                     /gene="<html><body><i>pac</i> from <i>Streptomyces
                     alboniger</i></body></html>"
                     /note="<html><body>confers resistance to
                     puromycin</body></html>"
                     /product="<html><body>puromycin
                     <i>N</i>-acetyltransferase</body></html>"
                     /codon_start=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /transl_table=1
                     /label="PuroR"
     primer_bind     complement(5072..5091)
                     /Tm=58
                     /Description="Puromycin resistance gene, reverse primer.
                     Also called puro-variant-R"
                     /label="Puro-R"
     primer_bind     5568..5588
                     /Tm=60
                     /Description="Puromycin resistance gene, forward primer"
                     /label="Puro-F"
     LTR             5809..6042
                     /note="<html><body>self-inactivating&nbsp;3' long terminal
                     repeat (LTR) from HIV-1</body></html>"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6114..6235
                     /note="<html><body>SV40 polyadenylation
                     signal</body></html>"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6151..6170)
                     /Tm=51
                     /Description="SV40 polyA, reverse primer"
                     /label="SV40pA-R"
     primer_bind     6205..6224
                     /Tm=54
                     /Description="SV40 polyA terminator, reverse primer"
                     /label="EBV-rev"
     rep_origin      6275..6410
                     /note="<html><body>SV40 origin of
                     replication</body></html>"
                     /label="SV40 ori"
     primer_bind     6337..6356
                     /Tm=56
                     /Description="SV40 promoter/origin, forward primer"
                     /label="SV40pro-F"
     primer_bind     complement(6430..6449)
                     /Tm=49
                     /Description="T7 promoter, forward primer"
                     /label="T7"
     promoter        complement(6431..6449)
                     /note="<html><body>promoter for bacteriophage T7 RNA
                     polymerase</body></html>"
                     /label="T7 promoter"
     primer_bind     complement(6459..6476)
                     /Tm=56
                     /Description="In lacZ gene. Also called M13-F20 or M13
                     (-21) Forward"
                     /label="M13 Forward"
     primer_bind     complement(6459..6475)
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /label="M13 fwd"
     primer_bind     complement(6468..6490)
                     /Tm=61
                     /Description="In lacZ gene"
                     /label="M13/pUC Forward"
     rep_origin      6617..7072
                     /note="<html><body>f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand
                     synthesis</body></html>"
                     /direction="RIGHT"
                     /label="f1 ori"
     primer_bind     complement(6704..6723)
                     /Tm=57
                     /Description="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     6914..6935
                     /Tm=57
                     /Description="F1 origin, forward primer"
                     /label="F1ori-F"
     promoter        7098..7202
                     /gene="<html><body><i>bla</i></body></html>"
                     /label="AmpR promoter"
     CDS             join(7203..7271,7272..8063)
                     /gene="<html><body><i>bla</i></body></html>"
                     /note="<html><body>confers resistance to ampicillin,
                     carbenicillin, and related antibiotics</body></html>"
                     /product="<html><body>β-lactamase</body></html>"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVG
                     YIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLV
                     EYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGP
                     LLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQI
                     AEIGASLIKHW*"
                     /transl_table=1
                     /label="AmpR"
     primer_bind     complement(7421..7440)
                     /Tm=58
                     /Description="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     rep_origin      8234..8822
                     /note="<html><body>high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication</body></html>"
                     /direction="RIGHT"
                     /label="ori"
     primer_bind     8723..8742
                     /Tm=56
                     /Description="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
ORIGIN      
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
      361 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
      421 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
      481 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
      541 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
      601 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
      661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1321 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1381 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1441 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1501 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1561 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1621 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1681 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1741 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1801 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1861 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1921 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1981 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2041 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2101 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2161 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     2221 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2281 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2821 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2881 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agtccagtgt
     2941 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag cggggacaag tttgtacaaa
     3001 aaagcaggct tcaccatgga ctacaaagac gatgacgaca aggcagccga gactcggaac
     3061 gtggccggag cagaggcccc accgccccag aagcgctact accggcaacg tgctcactcc
     3121 aaccccatgg cggaccacac gctgcgctac cctgtgaagc cagaggagat ggactggtct
     3181 gagctatacc cagagttctt cgctccactc actcaaaatc agagccacga cgatccgaaa
     3241 gacaaaaagg aaaagagagc tcaggcccaa gtggagtttg cagacatagg ctgtggctat
     3301 ggtggcctgt tagtggaact gtcaccgctg ttcccagaca cacttattct gggtctggag
     3361 atccgtgtta aagtgtctga ttacgtacaa gaccggattc gggccctacg cgcagctcct
     3421 gcaggtggct tccagaacat cgcctgtctc cgtagcaatg ccatgaagca cctgccgaat
     3481 tttttttata aaggccagct gacaaagatg ttcttcctct tccccgaccc gcactttaaa
     3541 cgtacgaaac acaagtggcg aatcatcagt cccaccctgc tagcagaata tgcctacgtg
     3601 ctaagagttg gggggctggt gtataccata accgatgtgc tggagctaca cgattggatg
     3661 tgtacccact ttgaagagca cccactgttt gagcgtgtgc ctctggagga cctgagtgaa
     3721 gaccccgttg tgggacatct aggcacctca actgaggagg ggaagaaagt tctacgtaat
     3781 ggagggaaga atttcccagc catcttccga agaatacaag atcccgtcct ccaggcagtg
     3841 acctcccaaa ccagcctgcc tggtcactga aaacccagct ttcttgtaca aagtggtcag
     3901 ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca atcaacctct
     3961 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4021 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4081 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4141 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4201 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4261 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4321 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     4381 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4441 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4501 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg
     4561 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     4621 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     4681 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     4741 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     4801 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     4861 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     4921 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     4981 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     5041 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     5101 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5161 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5221 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5281 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5341 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5401 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5461 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5521 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5581 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5641 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     5701 cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga ccaatgactt
     5761 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag
     5821 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5881 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5941 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6001 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6061 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6241 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6301 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6361 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6421 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6481 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6541 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6601 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6661 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6721 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6781 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6841 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6901 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6961 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7021 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7081 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7141 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7201 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7261 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7321 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7381 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7441 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7501 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7561 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7621 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7681 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7741 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7801 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7861 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7921 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7981 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8041 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8101 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8161 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8221 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8281 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8341 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8401 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8461 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8521 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8581 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8641 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8701 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8761 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8821 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8881 t
//
