LOCUS       pLenti_NFLAG_EGFP_Puro        8745 bp    DNA     circular UNA 14-JUN-2024
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV
            promoter, Puro.
ACCESSION   urn.local...6l7-hubxq57
VERSION     urn.local...6l7-hubxq57
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     95..112
                     /Tm=58
                     /Description="L4440 vector, forward primer"
                     /label="L4440"
     protein_bind    229..250
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /label="CAP binding site"
     promoter        join(265..270,271..288,289..295)
                     /note="<html><body>promoter for the <i>E. coli lac</i>
                     operon</body></html>"
                     /label="lac promoter"
     protein_bind    303..319
                     /note="<html><body>The <i>lac</i> repressor binds to the
                     <i>lac</i> operator to inhibit transcription in <i>E.
                     coli</i>. This inhibition can be relieved by adding
                     lactose or isopropyl-β-D-thiogalactopyranoside
                     (IPTG).</body></html>"
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by
                     <i>lacI</i></body></html>"
                     /label="lac operator"
     primer_bind     308..330
                     /Tm=58
                     /Description="In lacZ gene"
                     /label="M13/pUC Reverse"
     primer_bind     327..343
                     /Tm=49
                     /Description="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     327..343
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /label="M13 rev"
     primer_bind     362..382
                     /Tm=52
                     /Description="T3 promoter, forward primer"
                     /label="T3"
     promoter        364..382
                     /note="<html><body>promoter for bacteriophage T3 RNA
                     polymerase</body></html>"
                     /label="T3 promoter"
     promoter        410..636
                     /note="<html><body>Rous sarcoma virus
                     enhancer/promoter</body></html>"
                     /label="RSV promoter"
     LTR             637..817
                     /note="<html><body>truncated 5' long terminal repeat (LTR)
                     from HIV-1</body></html>"
                     /label="5' LTR (truncated)"
     misc_feature    864..989
                     /note="<html><body>packaging signal of human
                     immunodeficiency virus type 1</body></html>"
                     /label="HIV-1 Ψ"
     misc_feature    1482..1715
                     /note="<html><body>The Rev response element (RRE) of HIV-1
                     allows for Rev-dependent mRNA export from the nucleus to
                     the cytoplasm.</body></html>"
                     /label="RRE"
     misc_feature    2211..2327
                     /note="<html><body>central polypurine tract and central
                     termination sequence of HIV-1</body></html>"
                     /label="cPPT/CTS"
     enhancer        2350..2653
                     /note="<html><body>human cytomegalovirus immediate early
                     enhancer</body></html>"
                     /label="CMV enhancer"
     promoter        2654..2857
                     /note="<html><body>human cytomegalovirus (CMV) immediate
                     early promoter</body></html>"
                     /label="CMV promoter"
     primer_bind     2808..2828
                     /Tm=65
                     /Description="Human CMV immediate early promoter, forward
                     primer"
                     /label="CMV-F"
     primer_bind     2854..2878
                     /Tm=60
                     /Description="Human CMV promoter, forward primer"
                     /label="LNCX"
     misc_feature    2962..3015
                     /created_by="CH197744"
                     /label="attR1"
     gene            3016..3735
                     /created_by="CH197744"
                     /modified_by="CH197744"
                     /label="EGFP"
     misc_feature    complement(3736..3783)
                     /created_by="CH197744"
                     /modified_by="CH197744"
                     /label="attR2"
     misc_feature    3814..4402
                     /note="<html><body>woodchuck hepatitis
                     virus&nbsp;posttranscriptional regulatory
                     element</body></html>"
                     /label="WPRE"
     primer_bind     complement(3867..3887)
                     /Tm=55
                     /Description="WPRE, reverse primer"
                     /label="WPRE-R"
     CDS             complement(4285..4296)
                     /product="<html><body>Factor Xa recognition and cleavage
                     site</body></html>"
                     /codon_start=1
                     /translation="IEGR"
                     /transl_table=1
                     /label="Factor Xa site"
     promoter        4416..4915
                     /note="<html><body>mouse phosphoglycerate kinase 1
                     promoter</body></html>"
                     /label="PGK promoter"
     primer_bind     complement(4451..4474)
                     /Tm=69
                     /Description="Murine stem cell virus, reverse primer"
                     /label="MSCV-rev"
     primer_bind     4848..4867
                     /Tm=55
                     /Description="Mouse PGK promoter, forward primer"
                     /label="mPGK-F"
     CDS             4936..5535
                     /gene="<html><body><i>pac</i> from <i>Streptomyces
                     alboniger</i></body></html>"
                     /note="<html><body>confers resistance to
                     puromycin</body></html>"
                     /product="<html><body>puromycin
                     <i>N</i>-acetyltransferase</body></html>"
                     /codon_start=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /transl_table=1
                     /label="PuroR"
     primer_bind     complement(4936..4955)
                     /Tm=58
                     /Description="Puromycin resistance gene, reverse primer.
                     Also called puro-variant-R"
                     /label="Puro-R"
     primer_bind     5432..5452
                     /Tm=60
                     /Description="Puromycin resistance gene, forward primer"
                     /label="Puro-F"
     LTR             5673..5906
                     /note="<html><body>self-inactivating&nbsp;3' long terminal
                     repeat (LTR) from HIV-1</body></html>"
                     /label="3' LTR (ΔU3)"
     polyA_signal    5978..6099
                     /note="<html><body>SV40 polyadenylation
                     signal</body></html>"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6015..6034)
                     /Tm=51
                     /Description="SV40 polyA, reverse primer"
                     /label="SV40pA-R"
     primer_bind     6069..6088
                     /Tm=54
                     /Description="SV40 polyA terminator, reverse primer"
                     /label="EBV-rev"
     rep_origin      6139..6274
                     /note="<html><body>SV40 origin of
                     replication</body></html>"
                     /label="SV40 ori"
     primer_bind     6201..6220
                     /Tm=56
                     /Description="SV40 promoter/origin, forward primer"
                     /label="SV40pro-F"
     primer_bind     complement(6294..6313)
                     /Tm=49
                     /Description="T7 promoter, forward primer"
                     /label="T7"
     promoter        complement(6295..6313)
                     /note="<html><body>promoter for bacteriophage T7 RNA
                     polymerase</body></html>"
                     /label="T7 promoter"
     primer_bind     complement(6323..6340)
                     /Tm=56
                     /Description="In lacZ gene. Also called M13-F20 or M13
                     (-21) Forward"
                     /label="M13 Forward"
     primer_bind     complement(6323..6339)
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /label="M13 fwd"
     primer_bind     complement(6332..6354)
                     /Tm=61
                     /Description="In lacZ gene"
                     /label="M13/pUC Forward"
     rep_origin      6481..6936
                     /note="<html><body>f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand
                     synthesis</body></html>"
                     /direction="RIGHT"
                     /label="f1 ori"
     primer_bind     complement(6568..6587)
                     /Tm=57
                     /Description="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     6778..6799
                     /Tm=57
                     /Description="F1 origin, forward primer"
                     /label="F1ori-F"
     promoter        6962..7066
                     /gene="<html><body><i>bla</i></body></html>"
                     /label="AmpR promoter"
     CDS             join(7067..7135,7136..7927)
                     /gene="<html><body><i>bla</i></body></html>"
                     /note="<html><body>confers resistance to ampicillin,
                     carbenicillin, and related antibiotics</body></html>"
                     /product="<html><body>β-lactamase</body></html>"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVG
                     YIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLV
                     EYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGP
                     LLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQI
                     AEIGASLIKHW*"
                     /transl_table=1
                     /label="AmpR"
     primer_bind     complement(7285..7304)
                     /Tm=58
                     /Description="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     rep_origin      8098..8686
                     /note="<html><body>high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication</body></html>"
                     /direction="RIGHT"
                     /label="ori"
     primer_bind     8587..8606
                     /Tm=56
                     /Description="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
ORIGIN      
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
      361 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
      421 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
      481 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
      541 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
      601 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
      661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1321 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1381 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1441 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1501 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1561 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1621 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1681 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1741 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1801 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1861 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1921 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1981 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2041 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2101 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2161 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     2221 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2281 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2821 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2881 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agtccagtgt
     2941 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag cggggacaag tttgtacaaa
     3001 aaagcaggct tcaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3061 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3121 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     3181 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     3241 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3301 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3361 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3421 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     3481 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     3541 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3601 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     3661 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3721 gagctgtaca agtaaaaccc agctttcttg tacaaagtgg tcagctttct tgtacaaagt
     3781 ggttgatatc cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt
     3841 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3901 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3961 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4021 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4081 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4141 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4201 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4261 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4321 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4381 tccctttggg ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag
     4441 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     4501 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     4561 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     4621 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     4681 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     4741 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     4801 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     4861 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     4921 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4981 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     5041 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     5101 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     5161 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     5221 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     5281 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     5341 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     5401 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     5461 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     5521 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     5581 ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga
     5641 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
     5701 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5761 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5821 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5881 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5941 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6001 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6061 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6121 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6181 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6241 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6301 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6361 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6781 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6841 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6901 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6961 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7021 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7081 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7141 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7201 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7261 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7321 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7381 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7441 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7501 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7561 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7621 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7681 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7741 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7801 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7861 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7921 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7981 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8041 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8101 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8161 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8221 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8281 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8341 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8461 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8521 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8581 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8641 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8701 ggccttttta cggttcctgg ccttttgctg gccttttgct cacat
//
