LOCUS       pCBh-SpCas9(MRRWMR)-P2A-mTagBFP2_(RAS3579) 8619 bp    DNA     circular UNK 02-OCT-2024
DEFINITION  .
ACCESSION   pCBh-SpCas9(MRRWMR)-P2A-mTagBFP2_(RAS3579)
VERSION     pCBh-SpCas9(MRRWMR)-P2A-mTagBFP2_(RAS3579)
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /note="pLannotate"
                     /standard_name="AmpR"
     regulatory      complement(1032..1136)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
     misc_feature    complement(1207..1251)
                     /note="pLannotate"
                     /standard_name="bom"
     regulatory      1420..1705
                     /note="Geneious type: enhancer"
                     /note="pLannotate"
                     /standard_name="CMV enhancer"
     regulatory      1707..2219
                     /note="Geneious type: promoter"
                     /standard_name="chicken beta actin promoter"
     misc_feature    2228..2240
                     /note="Geneious type: motif"
                     /standard_name="Kozac"
     CDS             6341..6364
                     /standard_name="linker"
     CDS             6365..6415
                     /standard_name="BPNLS SV40)"
     CDS             6416..6427
                     /standard_name="linker"
     CDS             6428..6493
                     /note="pLannotate"
                     /standard_name="3xFLAG"
     CDS             6494..6502
                     /standard_name="linker"
     CDS             6503..6568
                     /note="pLannotate"
                     /standard_name="P2A"
     CDS             6569..7279
                     /note="pLannotate"
                     /standard_name="mTagBFP2"
     misc_feature    7331..7558
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /note="Geneious type: terminator"
                     /standard_name="bGH poly(A) signal"
     rep_origin      complement(8031..8619)
     motif           5639..5641
                     /standard_name="M1135"
     motif           5642..5644
                     /standard_name="R1136"
     motif           5888..5890
                     /standard_name="R1218"
     motif           5891..5893
                     /standard_name="W1219"
     motif           6239..6241
                     /standard_name="M1335"
     motif           6245..6247
                     /standard_name="R1337"
     CDS             2237..6340
                     /note="pLannotate"
                     /standard_name="SpCas9-MRRWMR"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgtctagat cagggtaccc gttacataac ttacggtaaa
     1441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat
     1501 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1561 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1621 cgcctggcat tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1681 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     1741 catctccccc cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1801 tgtgcagcga tgggggcggg gggggggggg gggggcgcgc gccaggcggg gcggggcggg
     1861 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
     1921 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     1981 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
     2041 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     2101 gcccttctcc tccgggctgt aattagctga gcaagaggta agggtttaag ggatggttgg
     2161 ttggtggggt attaatgttt aattacctgg agcacctgcc tgaaatcact ttttttcagg
     2221 ttggaccggc gccaccatgg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     2281 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     2341 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     2401 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     2461 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     2521 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     2581 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     2641 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     2701 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     2761 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     2821 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     2881 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     2941 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     3001 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     3061 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     3121 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     3181 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     3241 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     3301 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     3361 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     3421 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     3481 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     3541 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     3601 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     3661 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     3721 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     3781 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     3841 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     3901 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     3961 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     4021 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     4081 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     4141 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     4201 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     4261 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     4321 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     4381 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     4441 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     4501 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     4561 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     4621 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     4681 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     4741 ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     4801 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     4861 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     4921 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     4981 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     5041 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     5101 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     5161 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     5221 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     5281 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     5341 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     5401 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     5461 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     5521 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     5581 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcat
     5641 gagacccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     5701 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     5761 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     5821 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     5881 ctctgccaga tggctgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     5941 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     6001 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     6061 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     6121 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     6181 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagat
     6241 gtacagaagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     6301 gtacgagaca cggatcgacc tgtctcagct gggaggcgac ggatccggcg gaggcggaag
     6361 cgggaaaaga accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtctcgag
     6421 cggaggcgac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
     6481 tgacgatgac aagggaggcg gtggaagcgg cgcaacaaac ttctctctgc tgaaacaagc
     6541 cggagatgtc gaagagaatc ctggaccgat ggtgtctaag ggcgaagagc tgattaagga
     6601 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
     6661 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     6721 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
     6781 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
     6841 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     6901 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
     6961 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     7021 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
     7081 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
     7141 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
     7201 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
     7261 actggggcac aaacttaatt gaccggtcat catcaccatc accattgagt ttaaacccgc
     7321 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7381 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7441 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7501 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     7561 tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg
     7621 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7681 atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca
     7741 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7801 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7861 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7921 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7981 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8041 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8101 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8161 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8221 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8281 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8341 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8401 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8461 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8521 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8581 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa
//
