LOCUS       pAAV-TNT4-Zfr-P2A-HA-lu 7064 bp ds-DNA     circular     21-JUN-2024
DEFINITION  .
COMMENT     ^^
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /label="5' ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    29..34
                     /label="XmaI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    40..45
                     /label="XmaI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    176..197
                     /label="overlap"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          176..218
                     /label="cmv2TNT4-clone-F"
                     /note="sequence: TGGGGATCCTCTAGAACTATAGCTCAGTCCATTAGGAGCCAGT"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    178..197
                     /label="nhe1cut"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    198..218
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    198..741
                     /label="hTNT promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    737..756
                     /label="cut of not1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          737..777
                     /label="tnt4-zn-clone-F"
                     /note="sequence: GGCAGagctCCTCGAGAAGcACTGACCAACAGCGTGAAGAC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    757..777
                     /label="overlap"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    757..795
                     /label="upstream"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            757..795
                     /label="Zfr-201 Exon 19"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     gene            757..1989
                     /label="Zfr minigene(1233BP)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          769..792
                     /label="Zfr-rtpcr-F"
                     /note="sequence: CGTGAAGACATTACATCCAGTGCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    841..930
                     /label="I1D1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    841..1200
                     /label="to delete"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    931..1020
                     /label="I1D2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1021..1110
                     /label="I1D3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1111..1200
                     /label="I1D4"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1261..1264
                     /label="bp"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1296..1379
                     /label="PSEX"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1299..1379
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="KGLHIKNIV*TL*GQRSVLISPD*KL*"
     primer          1345..1369
                     /label="Zfr-qpcr-F"
                     /note="sequence: GAAGTGTGCTCATTTCTCCCGATTA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1376..1379
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="M"
     misc_feature    1380..1948
                     /label="keep"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1390..1431)
                     /label="tnt4-zn-clone-R"
                     /note="sequence: aaaccctgtttcaaaTCTCCCACCCCCAAAAGGCAAAATACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1412..1431
                     /label="overlap"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1412..1453
                     /label="zn-luci-clone-F"
                     /note="sequence: GGAGAtttgaaacagggtttctccttacagctctggctttcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1433..1522
                     /label="I2D1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1433..1882
                     /label="to delete"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1523..1612
                     /label="I2D2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1613..1702
                     /label="I2D3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1703..1792
                     /label="I2D4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1793..1882
                     /label="I2D5"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1933..1937
                     /label="bp"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1949..1989
                     /label="downstream"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            1949..1989
                     /label="Zfr-201 Exon 20"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1951..1989
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="CIETPCIPSDTQS"
     misc_feature    1966..1989
                     /label="overlap"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1990..2046
                     /label="P2A"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1990..2046
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer          complement(2003..2025)
                     /label="P2A-R1"
                     /note="sequence: gtctccagcctgTttTagTaggc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2005..2029)
                     /label="P2A-R2"
                     /note="sequence: cAacgtctccagcctgTttTagTag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2007..2028
                     /label="non-AUG start codons removed"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2011..2031)
                     /label="P2A-R5"
                     /note="sequence: ctcAacgtctccagcctgTtt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2017..2037)
                     /label="P2A-R3"
                     /note="sequence: gttctcctcAacgtctccagc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2025..2044)
                     /label="P2A-R4"
                     /note="sequence: gtccagggttctcctcAacg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2036..2077)
                     /label="zn-luci-clone-R"
                     /note="sequence: cttctGCGTAATCTGGAACGTCgTATGGATAaggtccagggt"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2047..2070
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="YPYDVPDY"
     misc_feature    2047..2073
                     /label="HA tag"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2047..2098
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="YPYDVPDYAEDAKNIKK"
     misc_feature    2059..2077
                     /label="OVERLAP"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2059..2101
                     /label="ha-luci-clone-F"
                     /note="sequence: GTTCCAGATTACGCagaagacgccaaaaacataaagaaaggcc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2074..2086
                     /label="Translation 2074-2086"
                     /translation="EDAK"
     CDS             2074..3722
                     /label="luciferase"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="EC_number: product: firefly luciferase codon_start: 1 translation: MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV* transl_table: 1 gene: luc+ note: enhanced luc+ version of the luciferase gene protein_id: "
     CDS             2074..3722
                     /label="Translation 2074-3722"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKMPC"
     primer          complement(2169..2188)
                     /label="DM-St-luci-qPCR-R1"
                     /note="sequence: ttgttccaggaaccagggcg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2541..2584)
                     /label="HA-luci-clone-R"
                     /note="sequence: gatgagatgtgacgaacgtgtacatcgactgaaatccctggtaa"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2566..2584
                     /label="cut of bsrg1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          3082..3104
                     /label="luciferase-F"
                     /note="sequence: caaggatatgggctcactgagac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3082..3303
                     /label="qpcr-sequence"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3190..3207)
                     /label="Lucifer-seq3"
                     /note="sequence: cccggtatccagatccac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3286..3303)
                     /label="luciferase-R"
                     /note="sequence: caaggcgttggtcgcttc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3704..3722
                     /label="overlap"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3715..3734
                     /label="cut"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             3715..3745
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="PCKYL*RMGT"
     primer          3715..3755
                     /label="luci-mir122-clone-F"
                     /note="sequence: ccgtgtaaGTACCTCTAGAGgatggggactgaaagcttcgg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3723..3727
                     /label="KpnI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3735..3755
                     /label="ol"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     source          3753..3839
                     /label="source"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="organism: synthetic DNA construct mol_type: other DNA"
     misc_feature    3757..3778
                     /label="miR122"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3785..3806
                     /label="miR122"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3813..3834
                     /label="miR122"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3835..3855
                     /label="ol"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3835..3875)
                     /label="mir122-clone-R"
                     /note="sequence: CGTCCTCGAGGCCGCCCGGGcggtatcgataagctTcggcg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3840..3844
                     /label="HindIII"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3856..3861
                     /label="XmaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3856..3875
                     /label="cut"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      3899..4025
                     /label="PolyA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4114..4243
                     /label="3' ITR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4199..4204
                     /label="XmaI"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4210..4215
                     /label="XmaI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      4420..4875
                     /label="f1 origin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4901..5005
                     /label="Amp Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5006..5863
                     /label="Amp resistent"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      6037..6625
                     /label="origin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG
      181 ATCCTCTAGA ACTATAGCTC AGTCCATTAG GAGCCAGTAG CCTGGAAGAT GTCTTTACCC
      241 CCAGCATCAG TTCAAGTGGA GCAGCACATA ACTCTTGCCC TCTGCCTTCC AAGATTCTGG
      301 TGCTGAGACT TATGGAGTGT CTTGGAGGTT GCCTTCTGCC CCCCAACCCT GCTCCCAGCT
      361 GGCCCTCCCA GGCCTGGGTT GCTGGCCTCT GCTTTATCAG GATTCTCAAG AGGGACAGCT
      421 GGTTTATGTT GCATGACTGT TCCCTGCATA TCTGCTCTGG TTTTAAATAG CTTATCTGAG
      481 CAGCTGGAGG ACCACATGGG CTTATATGGC GTGGGGTACA TGTTCCTGTA GCCTTGTCCC
      541 TGGCACCTGC CAAAATAGCA GCCAACAccc cccaccccca ccgccatccc cctgccccac
      601 ccgtccccTG TCGCACATTC CTCCCTCCGC AGGGCTGGCT CACCAGGCCC CAGCCCACAT
      661 GCCTGCTTAA AGCCCTCTCC ATCCTCTGCC TCACCCAGTC CCCGCTGAGA CTGAGCAGAC
      721 GCCTCCAGGA TCTGTCGGCA GagctCCTCG AGAAGcACTG ACCAACAGCG TGAAGACATT
      781 ACATCCAGTG CACAGGTAAT TGAATTCTTG TTGGATTACT GTCTCTAAGG CCTGGGAGTT
      841 TTATAATTGT TTCTTTATGT GTAATAATAA CCGTACTAGT CATTGTGATA CTTATTTGGG
      901 AAGTAAGCCT TTAAAGAGGT TATTAAAAAG ATTTACTGAT TCCCACACCG GAAGTTGCTC
      961 TTCTAGCCGT GAAAGAAGGA CGGCGTGGTC TTCAGTTTGC TATGTCCACT AACAATATGT
     1021 CCGACCCACG AAGGCCCACC AAAGTTATAC TTGTGGCACA TATAAACCTT TGGAATCAAG
     1081 GGACCTGATA CTGGATTATT AAATATTTAA GAGAATTTAT ATTTAATAAA CTTGATACTT
     1141 CAGTGAGAAA GGACAAATCA TCAGTAGAAT GGTTTTGAGA TAGCTGATGA TTCTATTAAA
     1201 AGTAGATATG TGTGTGGGTT AAATGACTTA AGCATACATC AAAAGACTGA GAAAGGCTCT
     1261 TAACATTAAA AGATGGGCCt ttttttttCT TTTAGGTTAA AGGATTACAT ATTAAAAATA
     1321 TTGTATAGAC TCTTTAAGGC CAAAGAAGTG TGCTCATTTC TCCCGATTAA AAGTTATGAG
     1381 TAAGAATTGG GTATTTTGCC TTTTGGGGGT GGGAGAtttg aaacagggtt tctccttaca
     1441 gctctggctt tcctggagca gtgaattgag cctggctcct ctgtaggagt aacgagttgc
     1501 tttaaccact gacatctttc caccaccacc accaccCCTT ATGAATAGAC TAAATTTATT
     1561 TAAAGATTGA TATTTTCTAG CTTAATTATT TGGAAAGCAA ATAAGCACAC AGAAAAGCCT
     1621 CCTCCCCaaa aaaatataaa taaaaaaGTC TATCAGGACA TAGTATATGT CCCTTCTGAT
     1681 TAATCATTGG TGGTGAAACA ACCCATTCAG GGTGTCTCTT CCATCACAGT GGAttttttt
     1741 CATATTGTTA CATGGAGAAG TTAACTTAGT AAGAATTGAT GTGCTTCTGT GCCAGTGAGA
     1801 TAGCTCTGCA GGTAAAAGGG AGACTGACCC CCAACTTGTA CTCTGGCCGT GGCAGTCATG
     1861 TGCACACAAG TAAACTGAAT AAATATAAAG AGCAGATTTT AGAGTGTTTT GGCTTTAAAT
     1921 TAATTCTATA TTCTAACTCT ATTTGCAGTT TGCATTGAGA CTCCTTGCAT TCCGTCAGAT
     1981 ACACAAAGTg ctactaactt cagcctActA aaAcaggctg gagacgtTga ggagaaccct
     2041 ggacctTATC CATAcGACGT TCCAGATTAC GCagaagacg ccaaaaacat aaagaaaggc
     2101 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
     2161 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
     2221 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
     2281 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     2341 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     2401 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
     2461 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
     2521 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     2581 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
     2641 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
     2701 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
     2761 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     2821 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     2881 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
     2941 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     3001 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
     3061 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
     3121 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     3181 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
     3241 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     3301 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     3361 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
     3421 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca
     3481 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     3541 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
     3601 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     3661 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatgccgtgt
     3721 aaGTACCTCT AGAGgatggg gactgaaagc ttcggtcaaa caccattgtc acactccata
     3781 ctatcaaaca ccattgtcac actccagatc aacaaacacc attgtcacac tccacgccgA
     3841 agcttatcga taccgCCCGG GCGGCCTCGA GGACGGGGTG AACTACGCCT GAGGATCCGA
     3901 TCTTTTTCCC TCTGCCAAAA ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC
     3961 TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC
     4021 ACTCGGAAGC AATTCGTTGA TCTGAATTTC GACCACCCAT AATACCCATT ACCCTGGTAG
     4081 ATAAGTAGCA TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA
     4141 CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC
     4201 CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCCTTAAT TAACCTAATT
     4261 CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC
     4321 GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC
     4381 GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA CGCGCCCTGT AGCGGCGCAT
     4441 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG
     4501 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC
     4561 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC
     4621 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT
     4681 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA
     4741 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG
     4801 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT
     4861 TAACGCTTAC AATTTAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT
     4921 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
     4981 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC
     5041 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA
     5101 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG
     5161 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT
     5221 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG
     5281 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC
     5341 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC
     5401 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA
     5461 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC
     5521 AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT
     5581 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA
     5641 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA
     5701 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA
     5761 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA
     5821 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT
     5881 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT
     5941 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG
     6001 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT
     6061 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA
     6121 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC
     6181 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC
     6241 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT
     6301 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG
     6361 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA
     6421 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT
     6481 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA
     6541 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC
     6601 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
     6661 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
     6721 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
     6781 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
     6841 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
     6901 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
     6961 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
     7021 CTATGACCAT GATTACGCCA GATTTAATTA AGGCCTTAAT TAGG
//