LOCUS       pAAV-TNT4-Zfr-P2A-HA-ya 6877 bp ds-DNA     circular     21-JUN-2024
DEFINITION  .
COMMENT     ^^
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /label="5' ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    29..34
                     /label="XmaI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    40..45
                     /label="XmaI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    176..197
                     /label="overlap"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    178..197
                     /label="nhe1cut"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    198..218
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    198..741
                     /label="hTNT promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    733..756
                     /label="NOT1 cut"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          737..777
                     /label="tnt4-zn-clone-F"
                     /note="sequence: GGCAGagctCCTCGAGAAGcACTGACCAACAGCGTGAAGAC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    757..777
                     /label="overlap"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    757..795
                     /label="upstream"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            757..795
                     /label="Zfr-201 Exon 19"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     gene            757..1989
                     /label="Zfr minigene(1233BP)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          769..792
                     /label="Zfr-rtpcr-F"
                     /note="sequence: CGTGAAGACATTACATCCAGTGCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    841..930
                     /label="I1D1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    841..1200
                     /label="to delete"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    931..1020
                     /label="I1D2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1021..1110
                     /label="I1D3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1111..1200
                     /label="I1D4"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1261..1264
                     /label="bp"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1296..1379
                     /label="PSEX"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1299..1379
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="KGLHIKNIV*TL*GQRSVLISPD*KL*"
     primer          1345..1369
                     /label="Zfr-qpcr-F"
                     /note="sequence: GAAGTGTGCTCATTTCTCCCGATTA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1376..1379
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="M"
     misc_feature    1380..1948
                     /label="keep"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1433..1522
                     /label="I2D1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1433..1882
                     /label="to delete"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1523..1612
                     /label="I2D2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1613..1702
                     /label="I2D3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1703..1792
                     /label="I2D4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1793..1882
                     /label="I2D5"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1933..1937
                     /label="bp"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1949..1989
                     /label="downstream"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            1949..1989
                     /label="Zfr-201 Exon 20"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1951..1989
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="CIETPCIPSDTQS"
     misc_feature    1966..1989
                     /label="overlap"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1990..2046
                     /label="P2A"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1990..2046
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer          complement(2003..2025)
                     /label="P2A-R1"
                     /note="sequence: gtctccagcctgTttTagTaggc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2005..2029)
                     /label="P2A-R2"
                     /note="sequence: cAacgtctccagcctgTttTagTag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2007..2028
                     /label="non-AUG start codons removed"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2011..2031)
                     /label="P2A-R5"
                     /note="sequence: ctcAacgtctccagcctgTtt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2017..2037)
                     /label="P2A-R3"
                     /note="sequence: gttctcctcAacgtctccagc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2025..2044)
                     /label="P2A-R4"
                     /note="sequence: gtccagggttctcctcAacg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2047..2070
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="YPYDVPDY"
     misc_feature    2047..2073
                     /label="HA tag"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2071..2089
                     /label="NOT1 cut"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2071..3543
                     /label="Translation 2071-3543"
                     /translation="ADPGQQPPPQPAPQGQGQPPSQPPQGQGPPSGPGQPAPAATQAAPQAPPAGHQIVHVRGDAETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQAATDAGTAGALTPQHVRAHAAPAALQLGAVSPGTLTPTGVVSGPAATPTAQHLRQAAFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDEATDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWLYL*"
     exon            2073..2391
                     /label="YAP1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             2073..3533
                     /label="YAP1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2251..2253
                     /label="YAP1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2257..2259
                     /label="YAP1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     PCR_primer      2266..2292
                     /label="hYAP-seq-F1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2383..2385
                     /label="YAP1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     exon            2392..2642
                     /label="YAP1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2395..2397
                     /label="YAP1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2398..2400
                     /label="YAP1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2425..2427
                     /label="YAP1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2449..2451
                     /label="YAP1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2449..2451
                     /label="Zhiqiang's S127A mutation"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2452..2454
                     /label="YAP1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2461..2463
                     /label="YAP1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2482..2484
                     /label="YAP1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2530..2532
                     /label="YAP1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2557..2559
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2560..2562
                     /label="YAP1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     exon            2643..2758
                     /label="YAP1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            2759..2872
                     /label="YAP1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     PCR_primer      2844..2873
                     /label="hYAP-Seq-F2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     exon            2873..3054
                     /label="YAP1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2890..2892
                     /label="YAP1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2935..2937
                     /label="YAP1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2941..3533
                     /label="YAP1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3053..3058
                     /label="skip one exon here in Zhiqiang's plasmid"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     exon            3055..3055
                     /label="YAP1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            3056..3185
                     /label="YAP1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3121..3123
                     /label="YAP1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3133..3135
                     /label="YAP1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3166..3168
                     /label="YAP1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            3186..3298
                     /label="YAP1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3211..3213
                     /label="YAP1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3220..3222
                     /label="YAP1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3229..3231
                     /label="YAP1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3241..3243
                     /label="YAP1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3256..3258
                     /label="YAP1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     exon            3299..3533
                     /label="YAP1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     PCR_primer      3370..3394
                     /label="hYAP-Seq-F3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3505..3533
                     /label="overlap"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3528..3547
                     /label="kpn1-CUT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          3528..3568
                     /label="mir122-clone-F"
                     /note="sequence: atggttGTACCTCTAGGTACgatggggactgaaagcttcgg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3548..3568
                     /label="ol"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3560..3560
                     /label="overlap"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3561..3565
                     /label="HindIII"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3566..3586
                     /label="PCR-mi122-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3566..3652
                     /label="合成"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     source          3566..3652
                     /label="source"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="organism: synthetic DNA construct mol_type: other DNA"
     misc_feature    3570..3591
                     /label="miR122"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3598..3619
                     /label="miR122"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3626..3647
                     /label="miR122"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3637..3652
                     /label="PCR-mi122-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3648..3668
                     /label="ol"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3648..3688)
                     /label="mir122-clone-R"
                     /note="sequence: CGTCCTCGAGGCCGCCCGGGcggtatcgataagctTcggcg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3653..3657
                     /label="HindIII"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3669..3688
                     /label="sal1-cut"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     terminator      3712..3838
                     /label="PolyA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(3743..3762)
                     /label="polyA-R"
                     /note="sequence: CTCAAGGGGCTTCATGATGT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3927..4056
                     /label="3' ITR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4012..4017
                     /label="XmaI"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4023..4028
                     /label="XmaI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      4233..4688
                     /label="f1 origin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4714..4818
                     /label="Amp Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4819..5676
                     /label="Amp resistent"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      5850..6438
                     /label="origin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG
      181 ATCCTCTAGA ACTATAGCTC AGTCCATTAG GAGCCAGTAG CCTGGAAGAT GTCTTTACCC
      241 CCAGCATCAG TTCAAGTGGA GCAGCACATA ACTCTTGCCC TCTGCCTTCC AAGATTCTGG
      301 TGCTGAGACT TATGGAGTGT CTTGGAGGTT GCCTTCTGCC CCCCAACCCT GCTCCCAGCT
      361 GGCCCTCCCA GGCCTGGGTT GCTGGCCTCT GCTTTATCAG GATTCTCAAG AGGGACAGCT
      421 GGTTTATGTT GCATGACTGT TCCCTGCATA TCTGCTCTGG TTTTAAATAG CTTATCTGAG
      481 CAGCTGGAGG ACCACATGGG CTTATATGGC GTGGGGTACA TGTTCCTGTA GCCTTGTCCC
      541 TGGCACCTGC CAAAATAGCA GCCAACAccc cccaccccca ccgccatccc cctgccccac
      601 ccgtccccTG TCGCACATTC CTCCCTCCGC AGGGCTGGCT CACCAGGCCC CAGCCCACAT
      661 GCCTGCTTAA AGCCCTCTCC ATCCTCTGCC TCACCCAGTC CCCGCTGAGA CTGAGCAGAC
      721 GCCTCCAGGA TCTGTCGGCA GagctCCTCG AGAAGcACTG ACCAACAGCG TGAAGACATT
      781 ACATCCAGTG CACAGGTAAT TGAATTCTTG TTGGATTACT GTCTCTAAGG CCTGGGAGTT
      841 TTATAATTGT TTCTTTATGT GTAATAATAA CCGTACTAGT CATTGTGATA CTTATTTGGG
      901 AAGTAAGCCT TTAAAGAGGT TATTAAAAAG ATTTACTGAT TCCCACACCG GAAGTTGCTC
      961 TTCTAGCCGT GAAAGAAGGA CGGCGTGGTC TTCAGTTTGC TATGTCCACT AACAATATGT
     1021 CCGACCCACG AAGGCCCACC AAAGTTATAC TTGTGGCACA TATAAACCTT TGGAATCAAG
     1081 GGACCTGATA CTGGATTATT AAATATTTAA GAGAATTTAT ATTTAATAAA CTTGATACTT
     1141 CAGTGAGAAA GGACAAATCA TCAGTAGAAT GGTTTTGAGA TAGCTGATGA TTCTATTAAA
     1201 AGTAGATATG TGTGTGGGTT AAATGACTTA AGCATACATC AAAAGACTGA GAAAGGCTCT
     1261 TAACATTAAA AGATGGGCCt ttttttttCT TTTAGGTTAA AGGATTACAT ATTAAAAATA
     1321 TTGTATAGAC TCTTTAAGGC CAAAGAAGTG TGCTCATTTC TCCCGATTAA AAGTTATGAG
     1381 TAAGAATTGG GTATTTTGCC TTTTGGGGGT GGGAGAtttg aaacagggtt tctccttaca
     1441 gctctggctt tcctggagca gtgaattgag cctggctcct ctgtaggagt aacgagttgc
     1501 tttaaccact gacatctttc caccaccacc accaccCCTT ATGAATAGAC TAAATTTATT
     1561 TAAAGATTGA TATTTTCTAG CTTAATTATT TGGAAAGCAA ATAAGCACAC AGAAAAGCCT
     1621 CCTCCCCaaa aaaatataaa taaaaaaGTC TATCAGGACA TAGTATATGT CCCTTCTGAT
     1681 TAATCATTGG TGGTGAAACA ACCCATTCAG GGTGTCTCTT CCATCACAGT GGAttttttt
     1741 CATATTGTTA CATGGAGAAG TTAACTTAGT AAGAATTGAT GTGCTTCTGT GCCAGTGAGA
     1801 TAGCTCTGCA GGTAAAAGGG AGACTGACCC CCAACTTGTA CTCTGGCCGT GGCAGTCATG
     1861 TGCACACAAG TAAACTGAAT AAATATAAAG AGCAGATTTT AGAGTGTTTT GGCTTTAAAT
     1921 TAATTCTATA TTCTAACTCT ATTTGCAGTT TGCATTGAGA CTCCTTGCAT TCCGTCAGAT
     1981 ACACAAAGTg ctactaactt cagcctActA aaAcaggctg gagacgtTga ggagaaccct
     2041 ggacctTATC CATAcGACGT TCCAGATTAC GCagatcccg ggcagcagcc gccgcctcaa
     2101 ccggcccccc agggccaagg gcagccgcct tcgcagcccc cgcaggggca gggcccgccg
     2161 tccggacccg ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc accccccgcc
     2221 gggcatcaga tcgtgcacgt ccgcggggac gcggagaccg acctggaggc gctcttcaac
     2281 gccgtcatga accccaagac ggccaacgtg ccccagaccg tgcccatgag gctccggaag
     2341 ctgcccgact ccttcttcaa gccgccggag cccaaatccc actcccgaca ggccgctact
     2401 gatgcaggca ctgcaggagc cctgactcca cagcatgttc gagctcatgc cgctccagct
     2461 gctctgcagt tgggagctgt ttctcctggg acactgaccc ccactggagt agtctctggc
     2521 ccagcagcta cacccacagc tcagcatctt cgacaggctg cttttgagat acctgatgat
     2581 gtacctctgc cagcaggttg ggagatggca aagacatctt ctggtcagag atacttctta
     2641 aatcacatcg atcagacaac aacatggcag gaccccagga aggccatgct gtcccagatg
     2701 aacgtcacag cccccaccag tccaccagtg cagcagaata tgatgaactc ggcttcaggt
     2761 cctcttcctg atggatggga acaagccatg actcaggatg gagaaattta ctatataaac
     2821 cataagaaca agaccacctc ttggctagac ccaaggcttg accctcgttt tgccatgaac
     2881 cagagaatca gtcagagtgc tccagtgaaa cagccaccac ccctggctcc ccagagccca
     2941 cagggaggcg tcatgggtgg cagcaactcc aaccagcagc aacagatgcg actgcagcaa
     3001 ctgcagatgg agaaggagag gctgcggctg aaacagcaag aactgcttcg gcaggAgtta
     3061 gccctgcgta gccagttacc aacactggag caggatggtg ggactcaaaa tccagtgtct
     3121 tctcccggga tgtctcagga attgagaaca atgacgacca atagctcaga tcctttcctt
     3181 aacagtggca cctatcactc tcgagatgag gctacagaca gtggactaag catgagcagc
     3241 tacagtgtcc ctcgaacccc agatgacttc ctgaacagtg tggatgagat ggatacaggt
     3301 gatactatca accaaagcac cctgccctca cagcagaacc gtttcccaga ctaccttgaa
     3361 gccattcctg ggacaaatgt ggaccttgga acactggaag gagatggaat gaacatagaa
     3421 ggagaggagc tgatgccaag tctgcaggaa gctttgagtt ctgacatcct taatgacatg
     3481 gagtctgttt tggctgccac caagctagat aaagaaagct ttcttacatg gttGTACCTC
     3541 TAGGTACgat ggggactgaa agcttcggtc aaacaccatt gtcacactcc atactatcaa
     3601 acaccattgt cacactccag atcaacaaac accattgtca cactccacgc cgAagcttat
     3661 cgataccgCC CGGGCGGCCT CGAGGACGGG GTGAACTACG CCTGAGGATC CGATCTTTTT
     3721 CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA
     3781 TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA
     3841 AGCAATTCGT TGATCTGAAT TTCGACCACC CATAATACCC ATTACCCTGG TAGATAAGTA
     3901 GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC
     3961 TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT
     4021 TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAACCTA ATTCACTGGC
     4081 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
     4141 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC
     4201 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAAGCGC
     4261 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC
     4321 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT
     4381 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA
     4441 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC
     4501 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT
     4561 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG
     4621 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT
     4681 TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC
     4741 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA
     4801 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT
     4861 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT
     4921 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC
     4981 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA
     5041 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC
     5101 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC
     5161 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC
     5221 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG
     5281 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC
     5341 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC
     5401 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT
     5461 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA
     5521 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC
     5581 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG
     5641 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA
     5701 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC
     5761 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA
     5821 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC
     5881 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA
     5941 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT
     6001 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC
     6061 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG
     6121 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG
     6181 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG
     6241 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC
     6301 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT
     6361 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
     6421 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
     6481 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT
     6541 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA
     6601 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG
     6661 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC
     6721 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
     6781 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC
     6841 CATGATTACG CCAGATTTAA TTAAGGCCTT AATTAGG
//