LOCUS       pFOXA2-T2A-mTagB        6995 bp DNA     circular SYN 25-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 24, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6995
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     CDS             complement(146..396)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQL
                     RSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICSDAA"
     primer_bind     357..417
                     /label=Fragment 1.FOR
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             397..1225
                     /codon_start=1
                     /gene="FOXA2"
                     /gene_synonym="HNF-3-beta; HNF3B; TCF3B"
                     /product="hepatocyte nuclear factor 3-beta isoform 2"
                     /label=FOXA2
                     /note="isoform 2 is encoded by transcript variant 2;
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS13147.1"
                     /db_xref="GeneID:3170"
                     /db_xref="HGNC:HGNC:5022"
                     /db_xref="MIM:600288"
                     /protein_id="NP_710141.1"
                     /translation="LSEIYQWIMDLFPFYRQNQQRWQNSIRHSLSFNDCFLKVPRSPDK
                     PGKGSFWTLHPDSGNMFENGCYLRRQKRFKCEKQLALKEAAGAAGSGKKAAAGAQASQA
                     QLGEAAGPASETPAGTESPHSSASPCQEHKRGGLGELKGTPAAALSPPEPAPSPGQQQQ
                     AAAHLLGPPHHPGLPPEAHLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLK
                     AYEQVMHYPGYGSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS"
     misc_feature    397..1225
                     /label=5' HA
     misc_feature    1042
                     /label=HES3 has a>g SNP here
     primer_bind     complement(1205..1245)
                     /label=Fragment 1.REV
     primer_bind     1206..1241
                     /label=Fragment 2.FOR
     misc_feature    1217..1225
                     /label=gRNA 1
     misc_feature    complement(1224..1225)
                     /label=gRNA 2
     CDS             1226..1279
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A - rare codons changed
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1278..1279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1280..1987
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     primer_bind     1962..1981
                     /label=NHa05 Geno TagBFP F
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     gene            2024..2057
                     /label=loxP
                     /note="loxP"
     gene            2116..2630
                     /label=PGK promoter
                     /note="PGK promoter"
     promoter        2122..2621
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     gene            2633..2698
                     /label=EM7 promoter
                     /note="EM7 promoter"
     promoter        2633..2680
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2699..3502
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2699..3499
                     /label=Neo
                     /note="Neo"
     gene            3516..3818
                     /label=bGHpA
                     /note="bGHpA"
     polyA_signal    3540..3764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3764..3783
                     /label=NHa05 Geno bGH pA F
     protein_bind    complement(3821..3854)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     gene            3821..3854
                     /label=loxP
                     /note="loxP"
     primer_bind     complement(3864..3906)
                     /label=Fragment 2.REV
     primer_bind     3867..3907
                     /label=Fragment 3.FOR
     primer_bind     complement(3870..3886)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(3886)
                     /label=NHa05 sgRNA 638
                     /note="https://wge.stemcell.sanger.ac.uk/crispr/1183464638"
     misc_feature    3886
                     /label=NHa05 sgRNA 644
                     /note="https://wge.stemcell.sanger.ac.uk/crispr/1183464644"
     gene            3886
                     /gene="SOX10"
                     /gene_synonym="DOM; PCWH; WS2E; WS4; WS4C"
                     /label=SOX10
                     /note="SRY-box transcription factor 10; Derived by
                     automated computational analysis using gene prediction 
                     method: BestRefSeq."
                     /db_xref="GeneID:6663"
                     /db_xref="HGNC:HGNC:11190"
                     /db_xref="MIM:602229"
     gene            complement(3886)
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;RPB14.4; 
                     RPB6; RPC15"
                     /label=POLR2F
                     /note="RNA polymerase II, I and III subunit F; Derived by 
                     automated computational analysis using gene prediction 
                     method: BestRefSeq."
                     /db_xref="GeneID:5435"
                     /db_xref="HGNC:HGNC:9193"
                     /db_xref="MIM:604414"
     gene            3887..4693
                     /gene="FOXA2"
                     /gene_synonym="HNF-3-beta; HNF3B; TCF3B"
                     /label=FOXA2
                     /note="forkhead box A2; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq."
                     /db_xref="GeneID:3170"
                     /db_xref="HGNC:HGNC:5022"
                     /db_xref="MIM:600288"
     mRNA            3887..4693
                     /gene="FOXA2"
                     /gene_synonym="HNF-3-beta; HNF3B; TCF3B"
                     /product="forkhead box A2, transcript variant 2"
                     /label=FOXA2 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:3170"
                     /db_xref="HGNC:HGNC:5022"
                     /db_xref="MIM:600288"
                     /transcript_id="NM_153675.3"
     mRNA            3887..4693
                     /gene="FOXA2"
                     /gene_synonym="HNF-3-beta; HNF3B; TCF3B"
                     /product="forkhead box A2, transcript variant 1"
                     /label=FOXA2 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /db_xref="Ensembl:ENST00000419308.7"
                     /db_xref="GeneID:3170"
                     /db_xref="HGNC:HGNC:5022"
                     /db_xref="MIM:600288"
                     /transcript_id="NM_021784.5"
     misc_feature    3887..4693
                     /label=3' HA
     misc_feature    complement(3887..3904)
                     /label=gRNA 2
     misc_feature    3887..3897
                     /label=gRNA 1
     CDS             3887..3889
                     /codon_start=1
                     /gene="FOXA2"
                     /gene_synonym="HNF-3-beta; HNF3B; TCF3B"
                     /product="hepatocyte nuclear factor 3-beta isoform 1"
                     /label=FOXA2
                     /note="isoform 1 is encoded by transcript variant 1;
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS46585.1"
                     /db_xref="Ensembl:ENSP00000400341.3"
                     /db_xref="GeneID:3170"
                     /db_xref="HGNC:HGNC:5022"
                     /db_xref="MIM:600288"
                     /protein_id="NP_068556.2"
                     /translation=""
     CDS             3887..3889
                     /codon_start=1
                     /gene="FOXA2"
                     /gene_synonym="HNF-3-beta; HNF3B; TCF3B"
                     /product="hepatocyte nuclear factor 3-beta isoform 2"
                     /label=FOXA2
                     /note="isoform 2 is encoded by transcript variant 2;
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS13147.1"
                     /db_xref="GeneID:3170"
                     /db_xref="HGNC:HGNC:5022"
                     /db_xref="MIM:600288"
                     /protein_id="NP_710141.1"
                     /translation=""
     primer_bind     complement(4671..4733)
                     /label=Fragment 3.REV
     CDS             complement(4694..4778)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREV"
     primer_bind     complement(4774..4790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4798..4814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4822..4828,4829..4846,4847..4852))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4867..4888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5176..5764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5935..6726,6727..6795))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6796..6900)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtggctg agcgagatct accagtggat
      421 catggacctc ttccccttct accggcagaa ccagcagcgc tggcagaact ccatccgcca
      481 ctcgctctcc ttcaacgact gtttcctgaa ggtgccccgc tcgcccgaca agcccggcaa
      541 gggctccttc tggaccctgc accctgactc gggcaacatg ttcgagaacg gctgctacct
      601 gcgccgccag aagcgcttca agtgcgagaa gcagctggcg ctgaaggagg ccgcaggcgc
      661 cgccggcagc ggcaagaagg cggccgccgg agcccaggcc tcacaggctc aactcgggga
      721 ggccgccggg ccggcctccg agactccggc gggcaccgag tcgcctcact cgagcgcctc
      781 cccgtgccag gagcacaagc gagggggcct gggagagctg aaggggacgc cggctgcggc
      841 gctgagcccc ccagagccgg cgccctctcc cgggcagcag cagcaggccg cggcccacct
      901 gctgggcccg ccccaccacc cgggcctgcc gcctgaggcc cacctgaagc cggaacacca
      961 ctacgccttc aaccacccgt tctccatcaa caacctcatg tcctcggagc agcagcacca
     1021 ccacagccac caccaccacc aaccccacaa aatggacctc aaggcctacg aacaggtgat
     1081 gcactacccc ggctacggtt cccccatgcc tggcagcttg gccatgggcc cggtcacgaa
     1141 caaaacgggc ctggacgcct cgcccctggc cgcagatacc tcctactacc agggggtgta
     1201 ctcccggccc attatgaact cctctgaggg caggggaagc ctgctgacat gcggggacgt
     1261 ggaggaaaat cccggccccg tgtctaaggg cgaagagctg attaaggaga acatgcacat
     1321 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     1381 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     1441 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     1501 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     1561 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
     1621 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     1681 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     1741 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     1801 cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     1861 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     1921 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     1981 gcttaattga gcccaattcc gatcatattc aataaccctt aatataactt cgtataatgt
     2041 atgctatacg aagttattag gtctgaagag gagtttacgt ccagccaagc tagcttggct
     2101 gcaggtcgtc gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     2161 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     2221 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     2281 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     2341 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     2401 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     2461 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     2521 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     2581 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ctgttgacaa
     2641 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     2701 gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     2761 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     2821 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     2881 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     2941 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     3001 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     3061 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     3121 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     3181 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     3241 gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     3301 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     3361 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     3421 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     3481 ccttcttgac gagttcttct gaggggatca attctctaga gctcgctgat cagcctcgac
     3541 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     3601 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     3661 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     3721 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     3781 aaccagctgg ggctcgacta gagcttgcgg aacccttaat ataacttcgt ataatgtatg
     3841 ctatacgaag ttattaggtc cctcgagggg atccactagt tctagataag aagacgacgg
     3901 cttcaggccc ggctaactct ggcaccccgg atcgaggaca agtgagagag caagtggggg
     3961 tcgagacttt ggggagacgg tgttgcagag acgcaaggga gaagaaatcc ataacacccc
     4021 caccccaaca cccccaagac agcagtcttc ttcacccgct gcagccgttc cgtcccaaac
     4081 agagggccac acagataccc cacgttctat ataaggagga aaacgggaaa gaatataaag
     4141 ttaaaaaaaa gcctccggtt tccactactg tgtagactcc tgcttcttca agcacctgca
     4201 gattctgatt tttttgttgt tgttgttctc ctccattgct gttgttgcag ggaagtctta
     4261 cttaaaaaaa aaaaaaaatt ttgtgagtga ctcggtgtaa aaccatgtag ttttaacaga
     4321 accagagggt tgtactattg tttaaaaaca ggaaaaaaaa taatgtaagg gtctgttgta
     4381 aatgaccaag aaaaagaaaa aaaaagcatt cccaatcttg acacggtgaa atccaggtct
     4441 cgggtccgat taatttatgg tttctgcgtg ctttatttat ggcttataaa tgtgtattct
     4501 ggctgcaagg gccagagttc cacaaatcta tattaaagtg ttatacccgg ttttatccct
     4561 tgaatctttt cttccagatt tttcttttct ttacttggct tacaaaatat acaggcttgg
     4621 aaattatttc aagaaggagg gagggatacc ctgtctggtt gcaggttgta ttttattttg
     4681 gcccagggag tgtgtacctc gcgaatgcat ctagatatcg gatcccgggc ccgtcgactg
     4741 cagaggcctg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4801 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4861 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4921 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4981 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5581 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5701 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5761 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5821 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5881 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5941 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6001 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6061 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6121 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6181 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6241 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6301 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6361 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6421 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6481 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6541 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6601 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6661 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6721 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6781 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6841 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6901 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6961 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
