LOCUS       pGBSedit_Sham_For_AddGene       11131 bp    DNA     circular UNA 11-JUL-2024
DEFINITION  Generated by Gibson/Homology Cloning (pJC005.em (digested by BtgZI)
            Fragment 2, pJC005 Sham Target Cassette).
ACCESSION   urn.local...j8-hy720p6
VERSION     urn.local...j8-hy720p6
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(<10765..11131,1..>10616)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /X-Transferred_From="pJC005.em (digested by BtgZI)
                     Fragment 2 -  CylE KO Construct for insert into pJC005"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; source synthetic DNA construct
                     (<a href=""urn:local:.:9o-gpba3j5"">pJC005.em (digested by
                     BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; source synthetic DNA construct
                     (<a href=""urn:local:.:17z-gmr0hlg"">pJC005.em (digested
                     by BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; source synthetic DNA construct
                     (<a href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; source synthetic DNA construct
                     (<a href=""urn:local:.:xn-gkqfp2u"">pJC005.em (digested by
                     BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CR_COH1_KO_Cassette_Colony_3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; source synthetic DNA construct
                     (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; source synthetic DNA construct
                     (<a href=""urn:local:.:9o-gpba3j5"">pJC005.em (digested by
                     BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; source synthetic DNA construct
                     (<a href=""urn:local:.:xn-gkqfp2u"">pJC005.em (digested by
                     BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; source synthetic DNA construct
                     (<a href=""urn:local:.:17z-gmr0hlg"">pJC005.em (digested
                     by BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; source synthetic DNA construct
                     (<a href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /Alternative_Match="100.00%; source synthetic DNA
                     construct (<a href=""urn:local:.:f7-gqwayv7"">pJC007 tet
                     promoter</a>)"
                     /Alternative_Match="100.00%; source synthetic DNA
                     construct (<a href=""urn:local:.:gc-gqwbn83"">pJC007
                     extraction</a>)"
                     /label="source synthetic DNA construct"
     orf             12..179
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:34b-c8zu34j"">pMBsacB</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; possible
                     omega family transcriptional repressor (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     possible omega family transcriptional repressor (<a
                     href=""urn:local:.:10n-gt5cvjv"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /label="possible omega family transcriptional repressor"
     primer_bind     17..54
                     /SerialCloner_Color="&hFF00FF"
                     /SerialCloner_Arrow="True"
                     /note="gene-specific Tm: 56.1  Ta: 57.1"
                     /note="overlap: attgggtaccgggccccccc"
                     /note="gene-specific primer: AGATGCTATAAAAGCAGC"
                     /X-Transferred_From="pSBMC (pMBsacB-mCherry)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Fwd (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Fwd (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Fwd (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Fwd (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Fwd (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /label="cylE_Barcode_Region-Fwd"
     primer_bind     complement(37..81)
                     /SerialCloner_Color="&h003300"
                     /SerialCloner_Arrow="True"
                     /note="gene-specific Tm: 61.0  Ta: 57.1"
                     /note="overlap: tggagctccaccgcggtggc"
                     /note="gene-specific primer: CCTCTTTACTTGTTCCGATAAAAAG"
                     /X-Transferred_From="pSBMC (pMBsacB-mCherry)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Rev (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Rev (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Rev (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Rev (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cylE_Barcode_Region-Rev (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /label="cylE_Barcode_Region-Rev"
     primer_bind     37..56
                     /Sequence="GTGCTGACTTGCACCATATC"
                     /Hairpin_Tm="52.6"
                     /Tm="56.9"
                     /Self_Dimer_Tm="11.4"
                     /%GC="50.0"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:79-ft1a98x"">p3012 w/GFP + PstI
                     +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm start codon confirm (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm start codon confirm (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm start codon confirm (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm start codon confirm (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm start codon confirm (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm start codon confirm (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm start codon confirm (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm start codon confirm (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm start codon confirm (<a
                     href=""urn:local:.:93-ft1fhm9"">3012 w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:wi-fvacily"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear (digested by BamHI, BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:vn-fvabwem"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:3w-fv1256p"">p3012 GA with GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     start codon confirm (<a
                     href=""urn:local:.:3w-fv1256p"">p3012 GA with GFP</a>)"
                     /label="Erm start codon confirm"
     primer_bind     144..153
                     /Sequence="GTGCAGTATC"
                     /Hairpin_Tm="None"
                     /Tm="25.3"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:79-ft1a98x"">p3012 w/GFP + PstI
                     +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ErmR start codon in this direction (3012 (digested by
                     SphI, BamHI) Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ErmR start codon in this direction (3012 (digested by
                     SphI, BamHI) Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ErmR start codon in this direction (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ErmR start codon in this direction (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ErmR start codon in this direction (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ErmR start codon in this direction (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ErmR start codon in this direction (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ErmR start codon in this direction (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ErmR start codon in this direction (<a
                     href=""urn:local:.:93-ft1fhm9"">3012 w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:wi-fvacily"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear (digested by BamHI, BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:vn-fvabwem"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ErmR
                     start codon in this direction (<a
                     href=""urn:local:.:3w-fv1256p"">p3012 GA with GFP</a>)"
                     /label="ErmR start codon in this direction"
     primer_bind     169..218
                     /note="GFP_fwd"
                     /note="gene-specific Tm: 58.2  Ta: 59.2"
                     /note="overlap: atagagtatgatggtgcatg"
                     /note="gene-specific primer:
                     GAATTCATTAAATTTAACTTTAAGAAGGAG"
                     /X-Transferred_From="New Assembly"
                     /X-Transferred_Similarity="62.00%"
                     /X-Primary_Match="62.00%; Adjusted-Interval-0:0/0; GFP fwd
                     primer bind (<a href=""urn:local:.:6n-fswcm0n"">New
                     Assembly</a>)"
                     /X-Alternative_Match="62.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a href=""urn:local:.:6h-fswcfnl"">New
                     Assembly</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GFP fwd
                     primer bind (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a href=""urn:local:.:5t-g30fg6k"">p3012
                     TIP 16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a href=""urn:local:.:vn-fvabwem"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a href=""urn:local:.:dh-gbasuap"">p3012
                     TIP 11 (original) GFP precursor_NEB (digested by NotI,
                     SphI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; GFP
                     fwd primer bind (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /label="GFP fwd primer bind"
     misc_feature    complement(193..218)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="26.9"
                     /Tm="53.8"
                     /Hairpin_Tm="32.4"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="7.8"
                     /Sequence="CTCCTTCTTAATTACAAATTTTTAGC"
                     /Product_Size=4216
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:34b-c8zu34j"">pMBsacB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:33y-c8ztvun"">pHY304_Cat</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a href=""urn:local:.:h-eq0qjpb"">3015b
                     with mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /label="pHY304_noERM GA R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(193..218)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="26.9"
                     /Tm="53.8"
                     /Hairpin_Tm="32.4"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTCCTTCTTAATTACAAATTTTTAGC"
                     /X-Transferred_From="pABC_ffluc_Erm"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA R (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /label="pHY304_noERM GA R"
     misc_feature    complement(194..225)
                     /Sequence="GTAATCACTCCTTCTTAATTACAAATTTTTAG"
                     /Hairpin_Tm="32.4"
                     /Tm="57.1"
                     /Self_Dimer_Tm="None"
                     /%GC="25.0"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a href=""urn:local:.:79-ft1a98x"">p3012
                     w/GFP + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 Frag Reverse (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 Frag Reverse (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 Frag Reverse (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 Frag Reverse (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 Frag Reverse (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 Frag Reverse (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 Frag Reverse (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 Frag Reverse (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 Frag Reverse (<a href=""urn:local:.:93-ft1fhm9"">3012
                     w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a href=""urn:local:.:5t-g30fg6k"">p3012 TIP
                     16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a href=""urn:local:.:vn-fvabwem"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     Frag Reverse (<a href=""urn:local:.:3w-fv1256p"">p3012 GA
                     with GFP</a>)"
                     /label="Cas9 Frag Reverse"
                     /note="Geneious type: primer_bind_reverse"
     orf             226..963
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /X-Transferred_From="pABC_ffluc_Erm"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; ORF 1
                     (frame 1); ermB (erythromycin resistance) (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ORF 11 (frame 2) (<a
                     href=""urn:local:.:a0-gurhjvc"">pORI23 (based on
                     high-quality MCS sequencing data) (digested by SalI) -
                     KG_pORI23_Sip_Promoter_C1_R-pORI23_MCS_R.ab1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermB (erythromycin resistance) (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermB (erythromycin resistance) (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermB (erythromycin resistance) (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermB (erythromycin resistance) (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermB (erythromycin resistance) (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermB (erythromycin resistance) (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermB (erythromycin resistance) (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermB (erythromycin resistance) (<a
                     href=""urn:local:.:fg-hhvb41a"">New Assembly</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ORF 1
                     (frame 1); ermB (erythromycin resistance) (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ORF
                     1 (frame 1); ermB (erythromycin resistance) (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ORF
                     11 (frame 2) (<a href=""urn:local:.:a0-gurhjvc"">pORI23
                     (based on high-quality MCS sequencing data) (digested by
                     SalI) - KG_pORI23_Sip_Promoter_C1_R-pORI23_MCS_R.ab1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ermB
                     (erythromycin resistance) (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ermB
                     (erythromycin resistance) (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ermB
                     (erythromycin resistance) (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ermB
                     (erythromycin resistance) (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ermB
                     (erythromycin resistance) (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ermB
                     (erythromycin resistance) (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ermB
                     (erythromycin resistance) (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /label="ORF 1 (frame 1); ermB (erythromycin resistance)"
     cds             226..963
                     /gene="ermAM"
                     /function="confers erythromycin resistance"
                     /note="ErmAM"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methylase"
                     /protein_id="AAB96789.1"
                     /db_xref="GI:2773394"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; ermAM
                     CDS (<a href=""urn:local:.:34b-c8zu34j"">pMBsacB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:34g-c8zu3ga"">pORI23
                     (based on high-quality MCS sequencing data)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:33t-c8ztve7"">pHY304
                     Genbank Submission</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ermAM
                     CDS (<a href=""urn:local:.:14x-gtbfk0r"">3015b with
                     mCherry SHAM Proto 08-06-2020 (digested by BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:13d-gl1gym0"">p3015b sham
                     w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:1dk-h0qw6u1"">pORI23
                     (based on high-quality MCS sequencing data)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a
                     href=""urn:local:.:1c-ha19yrx"">pDCcompHssS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:h-eq0qjpb"">3015b with
                     mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:5k-gh0jlcx"">p3015b sham
                     w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:61-gaahv0e"">3015b with
                     mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM CDS (<a href=""urn:local:.:jg-gh1eddv"">p3015b sham
                     w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /label="ermAM CDS"
     primer_bind     226..264
                     /Sequence="ATGAACAAAAATATAAAATATTCTCAAAACTTTTTAACG"
                     /created_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:79-ft1a98x"">p3012 w/GFP + PstI
                     +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:93-ft1fhm9"">3012 w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3007 GA
                     3-piece Erm Frag F (<a
                     href=""urn:local:.:wi-fvacily"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear (digested by BamHI, BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:vn-fvabwem"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:3w-fv1256p"">p3012 GA with GFP</a>)"
                     /label="3007 GA 3-piece Erm Frag F"
     primer_bind     complement(319..341)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="43.5"
                     /Tm="59.7"
                     /Hairpin_Tm="40.3"
                     /Self_Dimer_Tm="2.2"
                     /Sequence="CCTGTTCCAATTTCGTAAACGGT"
                     /X-Transferred_From="pABC_ffluc_Erm"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     deltaCyl DS confirm F (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; deltaCyl
                     DS confirm F (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     deltaCyl DS confirm F (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /label="deltaCyl DS confirm F"
     primer_bind     352..372
                     /Sequence="TTAACGACGAAACTGGCTAAA"
                     /Hairpin_Tm="None"
                     /Tm="55.9"
                     /Self_Dimer_Tm="None"
                     /%GC="38.1"
                     /created_by="primer3"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="cfbProm-eGFP into 3015-CPF GA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a href=""urn:local:.:357-c906sen"">cfbProm-eGFP
                     into 3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm qPCR F (<a href=""urn:local:.:32d-c8zthnm"">3015b Flex
                     sgRNA Deleted Cryptic Promoter</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Erm qPCR
                     F (<a href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry
                     SHAM Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a href=""urn:local:.:13d-gl1gym0"">p3015b sham w/
                     TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a href=""urn:local:.:h-eq0qjpb"">3015b with
                     mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/
                     TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a href=""urn:local:.:61-gaahv0e"">3015b with
                     mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a href=""urn:local:.:jg-gh1eddv"">p3015b sham w/
                     TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a href=""urn:local:.:7i-gh0m63n"">GA Predicted
                     Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR F (<a href=""urn:local:.:69-gh0jsjw"">p3015b sham w/
                     TIP dCas9 RBS</a>)"
                     /label="Erm qPCR F"
     dna_probe_bind  complement(438..461)
                     /Mismatches=0
                     /modified_by="User"
                     /created_by="primer3"
                     /%GC="33.3"
                     /Tm="57.1"
                     /Hairpin_Tm="29.9"
                     /Self_Dimer_Tm="11.2"
                     /Sequence="AAAGTGACACGAGTATTCAGTTTT"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Seq3b (<a href=""urn:local:.:34b-c8zu34j"">pMBsacB</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Seq3b (<a href=""urn:local:.:14x-gtbfk0r"">3015b with
                     mCherry SHAM Proto 08-06-2020 (digested by BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a href=""urn:local:.:h-eq0qjpb"">3015b with
                     mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a href=""urn:local:.:61-gaahv0e"">3015b
                     with mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /label="pHY304 Seq3b"
     primer_bind     complement(438..461)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="33.3"
                     /Tm="57.1"
                     /Hairpin_Tm="29.9"
                     /Self_Dimer_Tm="11.2"
                     /Sequence="AAAGTGACACGAGTATTCAGTTTT"
                     /X-Transferred_From="pABC_ffluc_Erm"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Seq3b (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Seq3b (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq3b (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /label="pHY304 Seq3b"
     misc_feature    complement(442..466)
                     /Sequence="GAATTAAAGTGACACGAGTATTCAG"
                     /Hairpin_Tm="None"
                     /Tm="56.3"
                     /Self_Dimer_Tm="15.4"
                     /%GC="36.0"
                     /created_by="primer3"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="cfbProm-eGFP into 3015-CPF GA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a href=""urn:local:.:357-c906sen"">cfbProm-eGFP
                     into 3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm qPCR R (<a href=""urn:local:.:32d-c8zthnm"">3015b Flex
                     sgRNA Deleted Cryptic Promoter</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Erm qPCR
                     R (<a href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry
                     SHAM Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a href=""urn:local:.:13d-gl1gym0"">p3015b sham w/
                     TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a href=""urn:local:.:h-eq0qjpb"">3015b with
                     mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/
                     TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a href=""urn:local:.:61-gaahv0e"">3015b with
                     mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a href=""urn:local:.:jg-gh1eddv"">p3015b sham w/
                     TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a href=""urn:local:.:7i-gh0m63n"">GA Predicted
                     Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     qPCR R (<a href=""urn:local:.:69-gh0jsjw"">p3015b sham w/
                     TIP dCas9 RBS</a>)"
                     /label="Erm qPCR R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    524..550
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="33.3"
                     /Tm="58.7"
                     /Hairpin_Tm="36.6"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTATTCCTTACCATTTAAGCACACAAA"
                     /X-Transferred_From="pABC_ffluc_Erm"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /label="TRS 2-Piece ERM F"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     524..550
                     /Sequence="GTATTCCTTACCATTTAAGCACACAAA"
                     /Hairpin_Tm="36.6"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="33.3"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a href=""urn:local:.:79-ft1a98x"">p3012
                     w/GFP + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a href=""urn:local:.:ab-ft1fro4"">p3012
                     GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a href=""urn:local:.:ab-ft1fro4"">p3012
                     GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a href=""urn:local:.:l4-ftqlegx"">p3012
                     GA with GFP extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a href=""urn:local:.:l4-ftqlegx"">p3012
                     GA with GFP extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a href=""urn:local:.:93-ft1fhm9"">3012
                     w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2-piece
                     GA Erm F (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a href=""urn:local:.:5t-g30fg6k"">p3012
                     TIP 16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a href=""urn:local:.:vn-fvabwem"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece GA Erm F (<a href=""urn:local:.:3w-fv1256p"">p3012
                     GA with GFP</a>)"
                     /label="2-piece GA Erm F"
     primer_bind     524..550
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="33.3"
                     /Tm="58.7"
                     /Hairpin_Tm="36.6"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GTATTCCTTACCATTTAAGCACACAAA"
                     /Product_Size=4275
                     /X-Transferred_From="pDCcompEV_promoter p3015"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TRS 2-Piece ERM F (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDCcompHssS_promoter p3015"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a href=""urn:local:.:fg-hhvb41a"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a
                     href=""urn:local:.:6g-gpaj3vp"">vPL3004</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a
                     href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a
                     href=""urn:local:.:hj-hiax3g7"">pDCcomp_stock1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a href=""urn:local:.:cm-hhrkx4b"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM F (<a href=""urn:local:.:lp-hiwlt8v"">New
                     Assembly</a>)"
                     /label="TRS 2-Piece ERM F"
     misc_feature    542..568
                     /Sequence="GCACACAAATTATTAAAAAAGTGGTTT"
                     /Hairpin_Tm="None"
                     /Tm="57.0"
                     /Self_Dimer_Tm="2.3"
                     /%GC="25.9"
                     /modified_by="User"
                     /X-Transferred_From="pCAM48 Transposon"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-Piece GA Long F (<a
                     href=""urn:local:.:e5-gvbajkj"">pCAM48 Transposon</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2-Piece
                     GA Long F (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-Piece GA Long F (<a
                     href=""urn:local:.:e5-gvbajkj"">pCAM48 Transposon</a>)"
                     /label="2-Piece GA Long F"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     542..568
                     /Sequence="GCACACAAATTATTAAAAAAGTGGTTT"
                     /Hairpin_Tm="None"
                     /Tm="57.0"
                     /Self_Dimer_Tm="2.3"
                     /%GC="25.9"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a href=""urn:local:.:79-ft1a98x"">p3012
                     w/GFP + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Long OH 2-piece GA F (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Long OH 2-piece GA F (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Long OH 2-piece GA F (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Long OH 2-piece GA F (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Long OH 2-piece GA F (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Long OH 2-piece GA F (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Long OH 2-piece GA F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Long OH 2-piece GA F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Long OH 2-piece GA F (<a
                     href=""urn:local:.:93-ft1fhm9"">3012 w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Long OH
                     2-piece GA F (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a href=""urn:local:.:5t-g30fg6k"">p3012
                     TIP 16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a href=""urn:local:.:vn-fvabwem"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Long
                     OH 2-piece GA F (<a href=""urn:local:.:3w-fv1256p"">p3012
                     GA with GFP</a>)"
                     /label="Long OH 2-piece GA F"
     primer_bind     565..584
                     /Sequence="GTTTTTGAAAGCCATGCGTC"
                     /Hairpin_Tm="None"
                     /Tm="56.8"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pDCcompEV_promoter p3015"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Short
                     OH 2-Piece (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDCcompHssS_promoter p3015"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Short OH
                     2-Piece (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (<a href=""urn:local:.:fg-hhvb41a"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (p3015b sham w/ TIP dCas9 REVCOM)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (p3015b sham w/ TIP dCas9 REVCOM)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (<a
                     href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (<a
                     href=""urn:local:.:hj-hiax3g7"">pDCcomp_stock1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece (<a href=""urn:local:.:cm-hhrkx4b"">New
                     Assembly</a>)"
                     /label="Short OH 2-Piece"
     primer_bind     565..584
                     /Sequence="GTTTTTGAAAGCCATGCGTC"
                     /Hairpin_Tm="None"
                     /Tm="56.8"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Short
                     OH 2-Piece  (<a href=""urn:local:.:79-ft1a98x"">p3012
                     w/GFP + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a href=""urn:local:.:93-ft1fhm9"">3012
                     w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Short OH
                     2-Piece  (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:vn-fvabwem"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Short OH 2-Piece  (<a
                     href=""urn:local:.:3w-fv1256p"">p3012 GA with GFP</a>)"
                     /label="Short OH 2-Piece "
     misc_feature    570..589
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="50.0"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.7"
                     /Sequence="TGAAAGCCATGCGTCTGACA"
                     /X-Transferred_From="pABC_ffluc_Erm"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     deltaCyl US confirm R (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; deltaCyl
                     US confirm R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     deltaCyl US confirm R (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /label="deltaCyl US confirm R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(571..591)
                     /Sequence="GATGTCAGACGCATGGCTTTC"
                     /Hairpin_Tm="None"
                     /Tm="59.9"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:79-ft1a98x"">p3012
                     w/GFP + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (3012 (digested by SphI, BamHI) Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (3012 (digested by SphI, BamHI) Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:ab-ft1fro4"">p3012 GA
                     with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:ab-ft1fro4"">p3012 GA
                     with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:l4-ftqlegx"">p3012 GA
                     with GFP extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:l4-ftqlegx"">p3012 GA
                     with GFP extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:93-ft1fhm9"">3012 w/
                     GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2-piece
                     Erm R (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:5t-g30fg6k"">p3012
                     TIP 16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:vn-fvabwem"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2-piece Erm R (<a href=""urn:local:.:3w-fv1256p"">p3012 GA
                     with GFP</a>)"
                     /label="2-piece Erm R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(571..591)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="52.4"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GATGTCAGACGCATGGCTTTC"
                     /Product_Size=5461
                     /X-Transferred_From="pDCcompEV_promoter p3015"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TRS 2-Piece ERM R (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDCcompHssS_promoter p3015"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:fg-hhvb41a"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a
                     href=""urn:local:.:6g-gpaj3vp"">vPL3004</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a
                     href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a
                     href=""urn:local:.:hj-hiax3g7"">pDCcomp_stock1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:cm-hhrkx4b"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:lp-hiwlt8v"">New
                     Assembly</a>)"
                     /label="TRS 2-Piece ERM R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(571..591)
                     /created_by="primer3"
                     /%GC="52.4"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GATGTCAGACGCATGGCTTTC"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:32c-c8zthnl"">3015
                     Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TRS 2-Piece ERM R (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TRS 2-Piece ERM R (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TRS 2-Piece ERM R (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TRS 2-Piece ERM R (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:h-eq0qjpb"">3015b
                     with mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:61-gaahv0e"">3015b
                     with mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TRS
                     2-Piece ERM R (<a href=""urn:local:.:j-fs5tu9o"">3010-flex
                     + mCherry Reporter</a>)"
                     /label="TRS 2-Piece ERM R"
     dna_probe_bind  complement(892..913)
                     /Mismatches=0
                     /modified_by="User"
                     /created_by="primer3"
                     /%GC="36.4"
                     /Tm="53.5"
                     /Hairpin_Tm="49.2"
                     /Self_Dimer_Tm="31.0"
                     /Sequence="GCTCATAAGTAACGGTACTTAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Seq2b (<a href=""urn:local:.:34b-c8zu34j"">pMBsacB</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Seq2b (<a href=""urn:local:.:14x-gtbfk0r"">3015b with
                     mCherry SHAM Proto 08-06-2020 (digested by BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a href=""urn:local:.:h-eq0qjpb"">3015b with
                     mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a href=""urn:local:.:61-gaahv0e"">3015b
                     with mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /label="pHY304 Seq2b"
     primer_bind     complement(892..913)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="36.4"
                     /Tm="53.5"
                     /Hairpin_Tm="49.2"
                     /Self_Dimer_Tm="31.0"
                     /Sequence="GCTCATAAGTAACGGTACTTAA"
                     /X-Transferred_From="pABC_ffluc_Erm"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Seq2b (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Seq2b (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Seq2b (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /label="pHY304 Seq2b"
     primer_bind     966..989
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="33.3"
                     /Tm="55.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="7.8"
                     /Sequence="CTATGAGTCGCTTTTGTAAATTTG"
                     /Product_Size=4216
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a
                     href=""urn:local:.:34b-c8zu34j"">pMBsacB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a
                     href=""urn:local:.:33y-c8ztvun"">pHY304_Cat</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a href=""urn:local:.:h-eq0qjpb"">3015b
                     with mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304_noERM GA F (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /label="pHY304_noERM GA F"
     primer_bind     complement(1019..1056)
                     /note="Rest of p3015b_rev"
                     /note="gene-specific Tm: 57.5  Ta: 58.5"
                     /note="overlap: tccttggaag"
                     /note="gene-specific primer: CTGTCAGTAGTATACCTAATAATTTATC"
                     /X-Transferred_From="NEBuilder p3015b TIP dCas9 w/ RBS &
                     aatII"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Rest
                     of p3015b rev primer bind (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Rest of p3015b rev primer bind (<a
                     href=""urn:local:.:im-gh1ckil"">Template p3015b TIP dCas9
                     w/ RBS</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Rest of
                     p3015b rev primer bind (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rest
                     of p3015b rev primer bind (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rest
                     of p3015b rev primer bind (<a
                     href=""urn:local:.:im-gh1ckil"">Template p3015b TIP dCas9
                     w/ RBS</a>)"
                     /label="Rest of p3015b rev primer bind"
     ligation        1027..1046
                     /X-Transferred_From="p3015b sham w/ TIP dCas9 RBS - Rest
                     of p3015b - p3015b sham w/ TIP dCas9 RBS - dCas9 +mCherry
                     (reversed) - p3015b sham w/ TIP dCas9 RBS - Synthesized
                     DNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:my-gh6iq5f"">p3015b sham
                     w/ TIP dCas9 RBS - Rest of p3015b - p3015b sham w/ TIP
                     dCas9 RBS - dCas9 +mCherry (reversed) - p3015b sham w/ TIP
                     dCas9 RBS - Synthesized DNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:l1-gh6g2on"">NEBuilder
                     p3015b TIP dCas9 w/ RBS & aatII</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Ligation
                     (<a href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:my-gh6iq5f"">p3015b sham
                     w/ TIP dCas9 RBS - Rest of p3015b - p3015b sham w/ TIP
                     dCas9 RBS - dCas9 +mCherry (reversed) - p3015b sham w/ TIP
                     dCas9 RBS - Synthesized DNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:l1-gh6g2on"">NEBuilder
                     p3015b TIP dCas9 w/ RBS & aatII</a>)"
                     /label="Ligation"
     primer_bind     1037..1065
                     /note="dCas9 + mCherry_fwd"
                     /note="gene-specific Tm: 59.2  Ta: 60.2"
                     /note="overlap: ctactgacag"
                     /note="gene-specific primer: CTTCCAAGGAGCTAAAGAG"
                     /X-Transferred_From="NEBuilder p3015b TIP dCas9 w/ RBS &
                     aatII"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; dCas9
                     + mCherry fwd primer bind (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     dCas9 + mCherry fwd primer bind (<a
                     href=""urn:local:.:im-gh1ckil"">Template p3015b TIP dCas9
                     w/ RBS</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; dCas9 +
                     mCherry fwd primer bind (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     dCas9 + mCherry fwd primer bind (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     dCas9 + mCherry fwd primer bind (<a
                     href=""urn:local:.:im-gh1ckil"">Template p3015b TIP dCas9
                     w/ RBS</a>)"
                     /label="dCas9 + mCherry fwd primer bind"
     primer_bind     complement(1041..1087)
                     /note="CAT (from pDC123)_rev"
                     /note="gene-specific Tm: 57.2  Ta: 58.2"
                     /note="overlap: agcgattctaagcgctaggg"
                     /note="spacer: gcggccgc"
                     /note="gene-specific primer: CTAAAGCACCCATTAGTTC"
                     /X-Transferred_From="p3012 +GFP +CAT(notI removable)"
                     /X-Transferred_Similarity="53.19%"
                     /X-Primary_Match="53.19%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:hx-ft1nswg"">p3012 +GFP +CAT(notI
                     removable)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:wi-fvacily"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear (digested by BamHI, BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:vn-fvabwem"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:dh-gbasuap"">p3012 TIP 11 (original)
                     GFP precursor_NEB (digested by NotI, SphI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:m3-fv6z0n5"">p3012 GFP
                     precursor_NEB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:1g-fwv1qej"">p3012 GFP precursor_NEB
                     (digested by SphI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:c8-gbabrez"">p3012 TIP 11 (original)
                     GFP precursor_NEB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:kb-fv5qpjw"">p3012 GFP precursor_NEB
                     (-4bp b4 tetO-2)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CAT
                     (from pDC123) rev primer bind (<a
                     href=""urn:local:.:21-g9q1urs"">p3012 GA with TIP
                     dCas9</a>)"
                     /label="CAT (from pDC123) rev primer bind"
     CDS             1068..1085
                     /created_by="gideo"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:79-ft1a98x"">p3012 w/GFP
                     + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     -erm GA forward (<a href=""urn:local:.:ab-ft1fro4"">p3012
                     GA with GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; -erm GA
                     forward (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:5t-g30fg6k"">p3012 TIP
                     16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:vn-fvabwem"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:3w-fv1256p"">p3012 GA
                     with GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:4e-guqe4cv"">p3012 GA
                     with GFP - p3012 remove GFP for dCas9 GA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:dh-gbasuap"">p3012 TIP
                     11 (original) GFP precursor_NEB (digested by NotI, SphI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:gh-fv5jgkb"">p3012 GA
                     with GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:m3-fv6z0n5"">p3012 GFP
                     precursor_NEB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; -erm
                     GA forward (<a href=""urn:local:.:1g-fwv1qej"">p3012 GFP
                     precursor_NEB (digested by SphI)</a>)"
                     /label="-erm GA forward"
     primer_bind     1068..1085
                     /note="p3012 GFP_fwd"
                     /note="gene-specific Tm: 62.2  Ta: 63.2"
                     /note="gene-specific primer: CCCTAGCGCTTAGAATCG"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; p3012
                     GFP fwd primer bind (<a
                     href=""urn:local:.:79-ft1a98x"">p3012 w/GFP + PstI
                     +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:hx-ft1nswg"">p3012 +GFP +CAT(notI
                     removable)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p3012
                     GFP fwd primer bind (<a
                     href=""urn:local:.:wi-fvacily"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear (digested by BamHI, BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:vn-fvabwem"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:dh-gbasuap"">p3012 TIP 11 (original)
                     GFP precursor_NEB (digested by NotI, SphI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:m3-fv6z0n5"">p3012 GFP
                     precursor_NEB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:1g-fwv1qej"">p3012 GFP precursor_NEB
                     (digested by SphI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:c8-gbabrez"">p3012 TIP 11 (original)
                     GFP precursor_NEB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:kb-fv5qpjw"">p3012 GFP precursor_NEB
                     (-4bp b4 tetO-2)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     p3012 GFP fwd primer bind (<a
                     href=""urn:local:.:hx-ft1nswg"">p3012 +GFP +CAT(notI
                     removable)</a>)"
                     /label="p3012 GFP fwd primer bind"
     primer_bind     complement(1098..1119)
                     /created_by="gideo"
                     /Sequence="CGACGATTATTGGACTGGATCG"
                     /Tm="59.0"
                     /%GC="50.0"
                     /Hairpin_Tm="43.7"
                     /Self_Dimer_Tm="None"
                     /modified_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3015b sham w/ TIP dCas9 RBS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Seq TIP
                     dCas9 into p3015b REV (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dCas9 into p3015b REV (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dCas9 into p3015b REV (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dCas9 into p3015b REV (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dCas9 into p3015b REV (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dCas9 into p3015b REV (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dCas9 into p3015b REV (<a
                     href=""urn:local:.:lg-gh6glbz"">p3015b sham w/ TIP dCas9
                     RBS - Rest of p3015b</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dCas9 into p3015b REV (<a
                     href=""urn:local:.:my-gh6iq5f"">p3015b sham w/ TIP dCas9
                     RBS - Rest of p3015b - p3015b sham w/ TIP dCas9 RBS -
                     dCas9 +mCherry (reversed) - p3015b sham w/ TIP dCas9 RBS -
                     Synthesized DNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dCas9 into p3015b REV (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dCas9 into p3015b REV (<a
                     href=""urn:local:.:154-gl1mv2p"">GA ORI into duel tet No
                     ORI PRODUCT</a>)"
                     /label="Seq TIP dCas9 into p3015b REV"
     primer_bind     complement(1098..1119)
                     /Sequence="CGACGATTATTGGACTGGATCG"
                     /Tm="59.0"
                     /%GC="50.0"
                     /Hairpin_Tm="43.7"
                     /Self_Dimer_Tm="None"
                     /created_by="gideo"
                     /X-Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dcas9 (<a href=""urn:local:.:61-gaahv0e"">3015b with
                     mCherry SHAM Proto 08-06-2020</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Seq TIP
                     dcas9 (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Seq
                     TIP dcas9 (<a href=""urn:local:.:61-gaahv0e"">3015b with
                     mCherry SHAM Proto 08-06-2020</a>)"
                     /label="Seq TIP dcas9"
     misc_feature    complement(1142..1162)
                     /Sequence="CCAAAAGTAGGTAATTTCACC"
                     /Hairpin_Tm="39.0"
                     /Tm="52.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.1"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:79-ft1a98x"">p3012 w/GFP + PstI
                     +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm Stop codon confirm (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm Stop codon confirm (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm Stop codon confirm (<a
                     href=""urn:local:.:aq-ft1ghkf"">(No ERM) p3012 GA with GFP
                     extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm Stop codon confirm (<a
                     href=""urn:local:.:aq-ft1ghkf"">(No ERM) p3012 GA with GFP
                     extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm Stop codon confirm (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm Stop codon confirm (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm Stop codon confirm (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm Stop codon confirm (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Erm Stop codon confirm (<a
                     href=""urn:local:.:93-ft1fhm9"">3012 w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Erm Stop
                     codon confirm (<a href=""urn:local:.:wi-fvacily"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear (digested by
                     BamHI, BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:vn-fvabwem"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:3w-fv1256p"">p3012 GA with GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Erm
                     Stop codon confirm (<a
                     href=""urn:local:.:3w-fv1256p"">p3012 GA with GFP</a>)"
                     /label="Erm Stop codon confirm"
                     /note="Geneious type: primer_bind_reverse"
     CDS             1289..1570
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="95.7"
                     /match_length="102.2"
                     /fragment="False"
                     /other="CDS"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; copR (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; copR
                     (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; copR
                     (<a href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; copR
                     (<a
                     href=""urn:local:.:mv-hiwsv0x"">pJC007_A909_CovR_398</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; copR
                     (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; copR
                     (<a
                     href=""urn:local:.:h2-hiaworf"">pJC007_A909_CovR_166</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; copR
                     (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; copR
                     (<a href=""urn:local:.:fq-hgb5znw"">pJC007_CovR166</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; copR
                     (<a href=""urn:local:.:7f-hgqv7f8"">pJC005_CydDC-CAT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; copR
                     (TH3-p3012_idCas9)"
                     /label="copR"
     primer_bind     1517..1536
                     /Sequence="GAACAAGTAACATTGGATTT"
                     /Hairpin_Tm="None"
                     /Tm="49.5"
                     /Self_Dimer_Tm="None"
                     /%GC="30.0"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:79-ft1a98x"">p3012 w/GFP + PstI
                     +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd Erm Sequence Forward (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd Erm Sequence Forward (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd Erm Sequence Forward (<a
                     href=""urn:local:.:aq-ft1ghkf"">(No ERM) p3012 GA with GFP
                     extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd Erm Sequence Forward (<a
                     href=""urn:local:.:aq-ft1ghkf"">(No ERM) p3012 GA with GFP
                     extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd Erm Sequence Forward (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd Erm Sequence Forward (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd Erm Sequence Forward (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd Erm Sequence Forward (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd Erm Sequence Forward (<a
                     href=""urn:local:.:93-ft1fhm9"">3012 w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2nd Erm
                     Sequence Forward (<a href=""urn:local:.:wi-fvacily"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear (digested by
                     BamHI, BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:9q-g0w2wva"">From R</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:vn-fvabwem"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     Erm Sequence Forward (<a
                     href=""urn:local:.:3w-fv1256p"">p3012 GA with GFP</a>)"
                     /label="2nd Erm Sequence Forward"
     misc_feature    1546..1576
                     /Sequence="ATCACACTACTCTTCTTTTGCCTATTATAAC"
                     /Hairpin_Tm="None"
                     /Tm="60.0"
                     /Self_Dimer_Tm="None"
                     /%GC="32.3"
                     /created_by="primer3"
                     /X-Transferred_From="p3012 GA with TIP dCas9"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:5l-gaahs9y"">p3012 GA
                     with TIP dCas9</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3015b sham w/ TIP dCas9 RBS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:dh-gbasuap"">p3012
                     TIP 11 (original) GFP precursor_NEB (digested by NotI,
                     SphI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:69-gh0jsjw"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:lg-gh6glbz"">p3015b
                     sham w/ TIP dCas9 RBS - Rest of p3015b</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:my-gh6iq5f"">p3015b
                     sham w/ TIP dCas9 RBS - Rest of p3015b - p3015b sham w/
                     TIP dCas9 RBS - dCas9 +mCherry (reversed) - p3015b sham w/
                     TIP dCas9 RBS - Synthesized DNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /label="Oh Primer Bind R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(1546..1576)
                     /created_by="primer3"
                     /%GC="32.3"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTTATAATAGGCAAAAGAAGAGTAGTGTGAT"
                     /X-Transferred_From="p3012 GA with TIP dCas9"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:5l-gaahs9y"">p3012 GA
                     with TIP dCas9</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3015b sham w/ TIP dCas9 RBS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:dh-gbasuap"">p3012
                     TIP 11 (original) GFP precursor_NEB (digested by NotI,
                     SphI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:69-gh0jsjw"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:lg-gh6glbz"">p3015b
                     sham w/ TIP dCas9 RBS - Rest of p3015b</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:my-gh6iq5f"">p3015b
                     sham w/ TIP dCas9 RBS - Rest of p3015b - p3015b sham w/
                     TIP dCas9 RBS - dCas9 +mCherry (reversed) - p3015b sham w/
                     TIP dCas9 RBS - Synthesized DNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /label="OH Primer Bind R"
     misc_feature    complement(1605..10708)
                     /Source="referenceToDocument: urn:local:.:ii-hgb88zn"
                     /X-Transferred_From="pJC005_CydDC-CAT_3piece (digested by
                     Esp3I) Fragment 2 - Annealed oligo product 2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005_CydDC-CAT_3piece (digested by Esp3I) Fragment 2 -
                     PJC005 CydDC gene (concatenated sequence 3) (<a
                     href=""urn:local:.:jw-hgb9zs5"">pJC005_CydDC-CAT_3piece
                     (digested by Esp3I) Fragment 2 - Annealed oligo product
                     2</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_COH_CovR_148"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005_CydDC-CAT_3piece (digested by Esp3I) Fragment 2 -
                     PJC005 CydDC gene (concatenated sequence 3) (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005_CydDC-CAT_3piece (digested by Esp3I) Fragment 2 -
                     PJC005 CydDC gene (concatenated sequence 3) (<a
                     href=""urn:local:.:jw-hgb9zs5"">pJC005_CydDC-CAT_3piece
                     (digested by Esp3I) Fragment 2 - Annealed oligo product
                     2</a>)"
                     /label="pJC005_CydDC-CAT_3piece (digested by Esp3I)
                     Fragment 2 - PJC005 CydDC gene (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     1937..1970
                     /note="cas9_fwd"
                     /note="gene-specific Tm: 54.3  Ta: 55.3"
                     /note="overlap: gtatgatggtgcatggcatg"
                     /note="gene-specific primer: ATGAATAAGCCATATTCAATAG"
                     /X-Transferred_From="p3012 GA with TIP dCas9"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a href=""urn:local:.:5l-gaahs9y"">p3012
                     GA with TIP dCas9</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3015b sham w/ TIP dCas9 RBS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cas9 fwd
                     primer bind (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a href=""urn:local:.:69-gh0jsjw"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a href=""urn:local:.:lg-gh6glbz"">p3015b
                     sham w/ TIP dCas9 RBS - Rest of p3015b</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a href=""urn:local:.:my-gh6iq5f"">p3015b
                     sham w/ TIP dCas9 RBS - Rest of p3015b - p3015b sham w/
                     TIP dCas9 RBS - dCas9 +mCherry (reversed) - p3015b sham w/
                     TIP dCas9 RBS - Synthesized DNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cas9
                     fwd primer bind (<a href=""urn:local:.:154-gl1mv2p"">GA
                     ORI into duel tet No ORI PRODUCT</a>)"
                     /label="cas9 fwd primer bind"
     CDS             1939..2238
                     /gene="repD"
                     /note="RepD"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein D"
                     /protein_id="AAB96787.1"
                     /db_xref="GI:2773392"
                     /X-Transferred_From="CR_COH1_KO_Cassette_Colony_3"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     repD CDS (<a href=""urn:local:.:cp-hcb1yvp"">pJC005 KO
                     CydDC Chloro Exchange</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     repD CDS (<a href=""urn:local:.:1dk-h0qw6u1"">pORI23
                     (based on high-quality MCS sequencing data)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     repD CDS (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; repD CDS
                     (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a href=""urn:local:.:14x-gtbfk0r"">3015b with
                     mCherry SHAM Proto 08-06-2020 (digested by BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a href=""urn:local:.:13d-gl1gym0"">p3015b sham w/
                     TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a href=""urn:local:.:1dk-h0qw6u1"">pORI23 (based on
                     high-quality MCS sequencing data)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP
                     dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repD
                     CDS (<a href=""urn:local:.:61-gaahv0e"">3015b with mCherry
                     SHAM Proto 08-06-2020</a>)"
                     /label="repD CDS"
     CDS             2281..3768
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="97.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; repS (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repS
                     (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repS
                     (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repS
                     (<a href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repS
                     (<a
                     href=""urn:local:.:mv-hiwsv0x"">pJC007_A909_CovR_398</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repS
                     (<a
                     href=""urn:local:.:nx-hgbd27z"">SC_pJC005_CovR_CompleteKO
                     (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repS
                     (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repS
                     (<a
                     href=""urn:local:.:7a-hgqv127"">TH4-pJC007_ddCas12</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repS
                     (<a
                     href=""urn:local:.:h2-hiaworf"">pJC007_A909_CovR_166</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; repS
                     (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /label="repS"
     gene            complement(<2336..>2355)
                     /gene="ermAM"
                     /X-Transferred_From="3012 (digested by SphI, BamHI)
                     Fragment 2"
                     /X-Transferred_Similarity="60.00%"
                     /X-Primary_Match="60.00%; Adjusted-Interval-0:0/0; ermAM
                     gene (3012 (digested by SphI, BamHI) Fragment 2)"
                     /X-Alternative_Match="60.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:ab-ft1fro4"">p3012 GA
                     with GFP</a>)"
                     /X-Alternative_Match="60.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:l4-ftqlegx"">p3012 GA
                     with GFP extraction</a>)"
                     /X-Alternative_Match="60.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="60.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:93-ft1fhm9"">3012 w/
                     GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ermAM
                     gene (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:9q-g0w2wva"">From
                     R</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:5t-g30fg6k"">p3012 TIP
                     16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:vn-fvabwem"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:5k-gh0jlcx"">p3015b sham
                     w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:dh-gbasuap"">p3012 TIP
                     11 (original) GFP precursor_NEB (digested by NotI, SphI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:jg-gh1eddv"">p3015b sham
                     w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ermAM gene (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /label="ermAM gene"
     misc_feature    complement(2607..2637)
                     /Sequence="ATCACACTACTCTTCTTTTGCCTATTATAAC"
                     /Hairpin_Tm="None"
                     /Tm="60.0"
                     /Self_Dimer_Tm="None"
                     /%GC="32.3"
                     /created_by="primer3"
                     /X-Transferred_From="3012 (digested by SphI, BamHI)
                     Fragment 1"
                     /X-Transferred_Similarity="45.16%"
                     /X-Primary_Match="45.16%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (3012 (digested by SphI, BamHI) Fragment 1)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (3012 (digested by SphI, BamHI) Fragment 1)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:93-ft1fhm9"">3012 w/
                     GFP</a>)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:93-ft1fhm9"">3012 w/
                     GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:wi-fvacily"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear (digested by
                     BamHI, BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:9q-g0w2wva"">From
                     R</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:5t-g30fg6k"">p3012
                     TIP 16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:vn-fvabwem"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:dh-gbasuap"">p3012
                     TIP 11 (original) GFP precursor_NEB (digested by NotI,
                     SphI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Oh
                     Primer Bind R (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /label="Oh Primer Bind R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2607..2637
                     /created_by="primer3"
                     /%GC="32.3"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTTATAATAGGCAAAAGAAGAGTAGTGTGAT"
                     /X-Transferred_From="3012 (digested by SphI, BamHI)
                     Fragment 1"
                     /X-Transferred_Similarity="45.16%"
                     /X-Primary_Match="45.16%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (3012 (digested by SphI, BamHI) Fragment 1)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (3012 (digested by SphI, BamHI) Fragment 1)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:93-ft1fhm9"">3012 w/
                     GFP</a>)"
                     /X-Alternative_Match="45.16%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:93-ft1fhm9"">3012 w/
                     GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:wi-fvacily"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear (digested by
                     BamHI, BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:9q-g0w2wva"">From
                     R</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:5t-g30fg6k"">p3012
                     TIP 16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:vn-fvabwem"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:dh-gbasuap"">p3012
                     TIP 11 (original) GFP precursor_NEB (digested by NotI,
                     SphI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; OH
                     Primer Bind R (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /label="OH Primer Bind R"
     primer_bind     2737..2756
                     /Sequence="GTCTATGTGACTTCAAAATC"
                     /Hairpin_Tm="42.2"
                     /Tm="49.5"
                     /Self_Dimer_Tm="None"
                     /%GC="35.0"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a href=""urn:local:.:79-ft1a98x"">p3012
                     w/GFP + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward [2] (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward [2] (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward [2] (<a
                     href=""urn:local:.:aq-ft1ghkf"">(No ERM) p3012 GA with GFP
                     extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward [2] (<a
                     href=""urn:local:.:aq-ft1ghkf"">(No ERM) p3012 GA with GFP
                     extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward [2] (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward [2] (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward [2] (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward [2] (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward [2] (<a
                     href=""urn:local:.:93-ft1fhm9"">3012 w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 4th Erm
                     Forward [2] (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a href=""urn:local:.:5t-g30fg6k"">p3012
                     TIP 16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a href=""urn:local:.:vn-fvabwem"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a href=""urn:local:.:3w-fv1256p"">p3012
                     GA with GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward [2] (<a href=""urn:local:.:3w-fv1256p"">p3012
                     GA with GFP</a>)"
                     /label="4th Erm Forward [2]"
     primer_bind     complement(2790..2828)
                     /Sequence="ATGAACAAAAATATAAAATATTCTCAAAACTTTTTAACG"
                     /created_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="51.28%"
                     /X-Primary_Match="51.28%; Adjusted-Interval-0:0/0; 3007 GA
                     3-piece Erm Frag F (<a
                     href=""urn:local:.:79-ft1a98x"">p3012 w/GFP + PstI
                     +BamHI</a>)"
                     /X-Alternative_Match="51.28%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="51.28%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="51.28%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="51.28%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="51.28%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="51.28%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="51.28%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="51.28%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="51.28%; Adjusted-Interval-0:0/0;
                     3007 GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:93-ft1fhm9"">3012 w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3007 GA
                     3-piece Erm Frag F (<a
                     href=""urn:local:.:wi-fvacily"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear (digested by BamHI, BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:vn-fvabwem"">p3012 GFP precursor_NEB
                     WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:dh-gbasuap"">p3012 TIP 11 (original)
                     GFP precursor_NEB (digested by NotI, SphI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3007
                     GA 3-piece Erm Frag F (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /label="3007 GA 3-piece Erm Frag F"
     primer_bind     2843..2864
                     /Sequence="GTAATCATTTTGGTATTGCTCG"
                     /Hairpin_Tm="None"
                     /Tm="53.9"
                     /Self_Dimer_Tm="None"
                     /%GC="36.4"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:79-ft1a98x"">p3012
                     w/GFP + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward (<a href=""urn:local:.:aq-ft1ghkf"">(No
                     ERM) p3012 GA with GFP extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward (<a href=""urn:local:.:aq-ft1ghkf"">(No
                     ERM) p3012 GA with GFP extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward (<a href=""urn:local:.:ab-ft1fro4"">p3012
                     GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward (<a href=""urn:local:.:ab-ft1fro4"">p3012
                     GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     4th Erm Forward (<a href=""urn:local:.:93-ft1fhm9"">3012
                     w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 4th Erm
                     Forward (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:5t-g30fg6k"">p3012 TIP
                     16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:vn-fvabwem"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:3w-fv1256p"">p3012 GA
                     with GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 4th
                     Erm Forward (<a href=""urn:local:.:3w-fv1256p"">p3012 GA
                     with GFP</a>)"
                     /label="4th Erm Forward"
     primer_bind     3725..3744
                     /Sequence="CTAAAACGGACACACAACTC"
                     /Hairpin_Tm="None"
                     /Tm="54.6"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /modified_by="User"
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:79-ft1a98x"">p3012
                     w/GFP + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5th Erm forward (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5th Erm forward (3012 (digested by SphI, BamHI) Fragment
                     2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5th Erm forward (<a href=""urn:local:.:aq-ft1ghkf"">(No
                     ERM) p3012 GA with GFP extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5th Erm forward (<a href=""urn:local:.:aq-ft1ghkf"">(No
                     ERM) p3012 GA with GFP extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5th Erm forward (<a href=""urn:local:.:ab-ft1fro4"">p3012
                     GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5th Erm forward (<a href=""urn:local:.:ab-ft1fro4"">p3012
                     GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5th Erm forward (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5th Erm forward (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     5th Erm forward (<a href=""urn:local:.:93-ft1fhm9"">3012
                     w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 5th Erm
                     forward (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:5t-g30fg6k"">p3012 TIP
                     16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:vn-fvabwem"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:3w-fv1256p"">p3012 GA
                     with GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 5th
                     Erm forward (<a href=""urn:local:.:3w-fv1256p"">p3012 GA
                     with GFP</a>)"
                     /label="5th Erm forward"
     rep_origin      3772..3828
                     /created_by="User"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="cfbProm-eGFP into 3015-CPF GA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Origin
                     of Replication (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Origin
                     of Replication (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Origin of Replication (<a
                     href=""urn:local:.:lg-gh6glbz"">p3015b sham w/ TIP dCas9
                     RBS - Rest of p3015b</a>)"
                     /label="Origin of Replication"
     misc_difference complement(4141..4158)
                     /created_by="gideo"
                     /modified_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3015b sham w/ TIP dCas9 RBS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:fg-hhvb41a"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:hj-hiax3g7"">pDCcomp_stock1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:cm-hhrkx4b"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Add
                     Missing ORI SHORT Annealing (<a
                     href=""urn:local:.:lp-hiwlt8v"">New Assembly</a>)"
                     /label="Add Missing ORI SHORT Annealing"
     primer_bind     complement(4157..4179)
                     /Sequence="CACGTAACCACAAGAACTAATCC"
                     /Tm="57.5"
                     /%GC="43.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="gideo"
                     /X-Transferred_From="pDCcompEV_promoter p3015"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Rev (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDCcompHssS_promoter p3015"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Rev (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:dh-gbasuap"">p3012 TIP 11
                     (original) GFP precursor_NEB (digested by NotI, SphI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:fg-hhvb41a"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:hj-hiax3g7"">pDCcomp_stock1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:cm-hhrkx4b"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     (<a href=""urn:local:.:lp-hiwlt8v"">New Assembly</a>)"
                     /label="Rev"
     primer_bind     complement(4157..4179)
                     /Sequence="CACGTAACCACAAGAACTAATCC"
                     /Tm="57.5"
                     /%GC="43.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="gideo"
                     /X-Transferred_From="pDCcompEV_promoter p3015"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Test F
                     (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Test F (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDCcompHssS_promoter p3015"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Test F
                     (<a href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Test
                     F (<a href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Test
                     F (<a href=""urn:local:.:fg-hhvb41a"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Test
                     F (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Test
                     F (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Test
                     F (<a href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Test
                     F (<a href=""urn:local:.:hj-hiax3g7"">pDCcomp_stock1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Test
                     F (<a href=""urn:local:.:cm-hhrkx4b"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Test
                     F (<a href=""urn:local:.:lp-hiwlt8v"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Test
                     F (<a href=""urn:local:.:2b-g304mha"">p3012 TIP 11
                     (original) GFP precursor_NEB Copy</a>)"
                     /label="Test F"
     primer_bind     4193..4219
                     /Sequence="AGTAGTGAATTTTTGATTTTTGGTGTG"
                     /Tm="57.9"
                     /%GC="29.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="7.4"
                     /Product_Size=225
                     /modified_by="gideo"
                     /X-Transferred_From="CR_COH1_KO_Cassette_Colony_3"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pORI23
                     MCS F (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a href=""urn:local:.:cp-hcb1yvp"">pJC005 KO
                     CydDC Chloro Exchange</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a href=""urn:local:.:1dk-h0qw6u1"">pORI23
                     (based on high-quality MCS sequencing data)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pORI23
                     MCS F (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a href=""urn:local:.:13d-gl1gym0"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a href=""urn:local:.:5t-g30fg6k"">p3012 TIP
                     16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a href=""urn:local:.:1dk-h0qw6u1"">pORI23
                     (based on high-quality MCS sequencing data)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /label="pORI23 MCS F"
     primer_bind     4193..4219
                     /Sequence="AGTAGTGAATTTTTGATTTTTGGTGTG"
                     /Tm="57.9"
                     /%GC="29.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="7.4"
                     /Product_Size=225
                     /modified_by="gideo"
                     /X-Transferred_From="pDCcompEV_promoter p3015"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pORI23
                     MCS F Use for Screening (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:9q-h6vyeky"">pDCcomp</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:22-hfq6gth"">pDC_HssRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:9m-h6vyefn"">pDCcomp</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:1c-ha19yrx"">pDCcompHssS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:ej-hcqxuvp"">pDC_HssS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:ai-h6vz7jg"">pDCcomp CydDC</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:1w-ha5lhbu"">pDC_HssRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:a2-h6vyruf"">pDCcomp HssRS</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDCcompHssS_promoter p3015"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pORI23
                     MCS F Use for Screening (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:1c-ha19yrx"">pDCcompHssS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:kl-hir897o"">pDCcomp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:bp-hhrk7lb"">pDCcompHssS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:ej-hcqxuvp"">pDC_HssS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:fg-hhvb41a"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pORI23 MCS F Use for Screening (<a
                     href=""urn:local:.:j-hfq3z6e"">pDC_HssRS-Heme_GFP</a>)"
                     /label="pORI23 MCS F Use for Screening"
     primer_bind     complement(4197..4219)
                     /Sequence="CACACCAAAAATCAAAAATTCAC"
                     /Tm="54.2"
                     /%GC="30.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry Placeholder"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Rev Bind
                     (<a href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     Bind (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     Bind (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /label="Rev Bind"
     note            4231..5753
                     /created_by="gideo"
                     /label="Piece2"
     promoter        4274..4290
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="58.6"
                     /fragment="True"
                     /other="promoter"
                     /X-Transferred_From="TH1-p3015b_original"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH1-p3015b_original)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH1-p3015b_original)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH2-p3015b_TN)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH2-p3015b_TN)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH3-p3012_idCas9)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH3-p3012_idCas9)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (<a
                     href=""urn:local:.:gv-hgb74jv"">SC_p3015b_Purple</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (<a
                     href=""urn:local:.:gv-hgb74jv"">SC_p3015b_Purple</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (<a
                     href=""urn:local:.:gz-hgb74jy"">SC_p3015b_White</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (<a
                     href=""urn:local:.:gz-hgb74jy"">SC_p3015b_White</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH1-p3015b_original)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH1-p3015b_original)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH2-p3015b_TN)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH2-p3015b_TN)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH3-p3012_idCas9)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH3-p3012_idCas9)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (<a
                     href=""urn:local:.:gv-hgb74jv"">SC_p3015b_Purple</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (<a
                     href=""urn:local:.:gv-hgb74jv"">SC_p3015b_Purple</a>)"
                     /label="tet promoter (fragment)"
     promoter        complement(4297..4313)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="58.6"
                     /fragment="True"
                     /other="promoter"
                     /X-Transferred_From="TH1-p3015b_original"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH1-p3015b_original)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH1-p3015b_original)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH2-p3015b_TN)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH2-p3015b_TN)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH3-p3012_idCas9)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (TH3-p3012_idCas9)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (<a
                     href=""urn:local:.:gv-hgb74jv"">SC_p3015b_Purple</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (<a
                     href=""urn:local:.:gv-hgb74jv"">SC_p3015b_Purple</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (<a
                     href=""urn:local:.:gz-hgb74jy"">SC_p3015b_White</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet promoter (fragment) (<a
                     href=""urn:local:.:gz-hgb74jy"">SC_p3015b_White</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH1-p3015b_original)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH1-p3015b_original)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH2-p3015b_TN)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH2-p3015b_TN)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH3-p3012_idCas9)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (TH3-p3012_idCas9)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (<a
                     href=""urn:local:.:gv-hgb74jv"">SC_p3015b_Purple</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     promoter (fragment) (<a
                     href=""urn:local:.:gv-hgb74jv"">SC_p3015b_Purple</a>)"
                     /label="tet promoter (fragment)"
     CDS             complement(4318..4328)
                     /created_by="gideo"
                     /X-Transferred_From="pDCcompEV_promoter p3015"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     primer (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Rev primer (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDCcompHssS_promoter p3015"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     primer (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     primer (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     primer (<a href=""urn:local:.:gh-fv5jgkb"">p3012 GA with
                     GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     primer (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     primer (<a
                     href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Rev
                     primer (<a
                     href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /label="Rev primer"
     primer_bind     complement(4325..4345)
                     /Sequence="GATTTCGTAAGGATCCCCGGG"
                     /Hairpin_Tm="37.6"
                     /Tm="60.3"
                     /Self_Dimer_Tm="15.8"
                     /%GC="57.1"
                     /modified_by="User"
                     /X-Transferred_From="3010-flex + mCherry Reporter"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pcas9 sequence (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd pcas9 sequence (<a
                     href=""urn:local:.:6g-gpaj3vp"">vPL3004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd pcas9 sequence (<a
                     href=""urn:local:.:8w-gpayre3"">vPL3004 ORI?</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pcas9 sequence (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pcas9 sequence (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pcas9 sequence (<a
                     href=""urn:local:.:6g-gpaj3vp"">vPL3004</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pcas9 sequence (<a href=""urn:local:.:8w-gpayre3"">vPL3004
                     ORI?</a>)"
                     /label="2nd pcas9 sequence"
     misc_feature    complement(4384..4405)
                     /Sequence="CTTTTATCATTCTACATTTAGG"
                     /Hairpin_Tm="None"
                     /Tm="48.2"
                     /Self_Dimer_Tm="None"
                     /%GC="27.3"
                     /created_by="primer3"
                     /X-Transferred_From="3010-flex + mCherry Reporter"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pCas9 2nd attempt  (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd pCas9 2nd attempt  (<a
                     href=""urn:local:.:6g-gpaj3vp"">vPL3004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2nd pCas9 2nd attempt  (<a
                     href=""urn:local:.:8w-gpayre3"">vPL3004 ORI?</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pCas9 2nd attempt  (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pCas9 2nd attempt  (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pCas9 2nd attempt  (<a
                     href=""urn:local:.:6g-gpaj3vp"">vPL3004</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 2nd
                     pCas9 2nd attempt  (<a
                     href=""urn:local:.:8w-gpayre3"">vPL3004 ORI?</a>)"
                     /label="2nd pCas9 2nd attempt "
                     /note="Geneious type: primer_bind_reverse"
     misc_RNA        complement(4505..4583)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="97.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_RNA"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tracrRNA
                     (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tracrRNA (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tracrRNA (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tracrRNA (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tracrRNA (<a
                     href=""urn:local:.:mv-hiwsv0x"">pJC007_A909_CovR_398</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tracrRNA (<a
                     href=""urn:local:.:nx-hgbd27z"">SC_pJC005_CovR_CompleteKO
                     (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tracrRNA (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tracrRNA (<a
                     href=""urn:local:.:7a-hgqv127"">TH4-pJC007_ddCas12</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tracrRNA (<a
                     href=""urn:local:.:h2-hiaworf"">pJC007_A909_CovR_166</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tracrRNA (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /label="tracrRNA"
     primer_bind     4622..4641
                     /modified_by="User"
                     /Mismatch_Positions="CG=T===C====T=G=ACG="
                     /Mismatches=9
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGCTGGGCATAATGGAACGA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009 GA
                     Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /label="3009 GA Product - cfb-TetR Confirm F"
     primer_bind     4647..4666
                     /modified_by="User"
                     /Mismatch_Positions="CGC==G===T==T=GA=CG="
                     /Mismatches=10
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGCTGGGCATAATGGAACGA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009 GA
                     Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /label="3009 GA Product - cfb-TetR Confirm F"
     primer_bind     4658..4677
                     /modified_by="User"
                     /Mismatch_Positions="C=CTGG===T==T=GA=C=="
                     /Mismatches=10
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGCTGGGCATAATGGAACGA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009 GA
                     Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /label="3009 GA Product - cfb-TetR Confirm F"
     misc_feature    4691..5305
                     /note="replicon of pMB1"
                     /X-Transferred_From="pUC57 with TRS+mCherry"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (<a href=""urn:local:.:2w-f8azgmh"">pUC57
                     with TRS+mCherry</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (<a href=""urn:local:.:2f-f8azfro"">pUC57
                     with TRS+mCherry</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (<a href=""urn:local:.:1b-f8a951f"">pUC57
                     (modified)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (pUC57 with TRS+mCherry (digested by
                     XmaI, SacI) Fragment 3)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (<a href=""urn:local:.:4w-fg0m7ah"">pUC57
                     with TRS+mCherry</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; replicon
                     of pMB1 (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (<a href=""urn:local:.:2w-f8azgmh"">pUC57
                     with TRS+mCherry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (<a href=""urn:local:.:2f-f8azfro"">pUC57
                     with TRS+mCherry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (<a href=""urn:local:.:1b-f8a951f"">pUC57
                     (modified)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (pUC57 with TRS+mCherry (digested by
                     XmaI, SacI) Fragment 3)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     replicon of pMB1 (<a href=""urn:local:.:4w-fg0m7ah"">pUC57
                     with TRS+mCherry</a>)"
                     /label="replicon of pMB1"
     rep_origin      complement(4706..5294)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     source          complement(<4706..>5294)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ORI"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; source
                     synthetic DNA construct (<a
                     href=""urn:local:.:89-fs11m2o"">pUC ORI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     source synthetic DNA construct (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /label="source synthetic DNA construct"
     primer_bind     complement(4786..4805)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     4948..4967
                     /modified_by="User"
                     /Mismatch_Positions="C=======ATAA==G=ACG="
                     /Mismatches=9
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGCTGGGCATAATGGAACGA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009 GA
                     Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /label="3009 GA Product - cfb-TetR Confirm F"
     primer_bind     4986..5005
                     /modified_by="User"
                     /Mismatch_Positions="=====G==A=A==GGAACG="
                     /Mismatches=9
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGCTGGGCATAATGGAACGA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009 GA
                     Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /label="3009 GA Product - cfb-TetR Confirm F"
     rep_origin      <5150..5305
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="Possible 2k end from F"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322
                     Gram-negative Ori (<a
                     href=""urn:local:.:6x-g0w1lg6"">Possible 2k end from
                     F</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:72/0;
                     pBR322 Gram-negative Ori (<a
                     href=""urn:local:.:9x-g0w2z33"">From F</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:143/0;
                     pBR322 Gram-negative Ori (<a
                     href=""urn:local:.:4t-g0w0spy"">p3012 GFP precursor_NEB
                     extraction</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322
                     Gram-negative Ori (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322 Gram-negative Ori (<a
                     href=""urn:local:.:6x-g0w1lg6"">Possible 2k end from
                     F</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:72/0;
                     pBR322 Gram-negative Ori (<a
                     href=""urn:local:.:9x-g0w2z33"">From F</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:143/0;
                     pBR322 Gram-negative Ori (<a
                     href=""urn:local:.:4t-g0w0spy"">p3012 GFP precursor_NEB
                     extraction</a>)"
                     /label="pBR322 Gram-negative Ori"
     primer_bind     5165..5184
                     /modified_by="User"
                     /Mismatch_Positions="====GG==AT==TGG=ACG="
                     /Mismatches=10
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGCTGGGCATAATGGAACGA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009 GA
                     Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     GA Product - cfb-TetR Confirm F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /label="3009 GA Product - cfb-TetR Confirm F"
     primer_bind     5262..5281
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="50.0"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="25.7"
                     /Sequence="CAGCAGATTACGCGCAGAAA"
                     /Product_Size=410
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a href=""urn:local:.:32c-c8zthnl"">3015
                     Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 F (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 F (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 F (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 F (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 F (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009 cfb
                     Confirm 2 F (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a href=""urn:local:.:h-eq0qjpb"">3015b
                     with mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a href=""urn:local:.:61-gaahv0e"">3015b
                     with mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 F (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /label="3009 cfb Confirm 2 F"
     primer_bind     complement(5329..5350)
                     /created_by="gideo"
                     /Sequence="CGTGAGTTTTCGTTCCACTGAG"
                     /Tm="59.8"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="gideo"
                     /X-Transferred_From="pJC007 Double dCas12 mutations"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pJC007
                     D908A GA1 Rev (pJC007 Double dCas12 mutations)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC007 D908A GA1 Rev (<a
                     href=""urn:local:.:v5-gz1iqqn"">pJC007 Double dCas12
                     mutations</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pJC007
                     D908A GA1 Rev (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC007 D908A GA1 Rev (pJC007 Double dCas12 mutations)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC007 D908A GA1 Rev (<a
                     href=""urn:local:.:v5-gz1iqqn"">pJC007 Double dCas12
                     mutations</a>)"
                     /label="pJC007 D908A GA1 Rev"
     primer_bind     complement(5345..5368)
                     /%GC="41.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.3"
                     /created_by="primer3"
                     /Sequence="ATGACCAAAATCCCTTAACGTGAG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry Placeholder"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3015b KG
                     PS Screen R (<a href=""urn:local:.:h-eq0qjpb"">3015b with
                     mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3015b KG PS Screen R (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:v-eq0qxqg"">3015b KG PS Screen R</a>"
                     /label="3015b KG PS Screen R"
     primer_bind     5349..5371
                     /created_by="gideo"
                     /Sequence="CGTTAAGGGATTTTGGTCATGAG"
                     /Tm="57.8"
                     /%GC="43.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="gideo"
                     /X-Transferred_From="pJC007 Double dCas12 mutations"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pJC007
                     D908A GA2 Fwd (pJC007 Double dCas12 mutations)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC007 D908A GA2 Fwd (<a
                     href=""urn:local:.:v5-gz1iqqn"">pJC007 Double dCas12
                     mutations</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pJC007
                     D908A GA2 Fwd (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC007 D908A GA2 Fwd (pJC007 Double dCas12 mutations)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC007 D908A GA2 Fwd (<a
                     href=""urn:local:.:v5-gz1iqqn"">pJC007 Double dCas12
                     mutations</a>)"
                     /label="pJC007 D908A GA2 Fwd"
     CDS             5470..5544
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="96.0"
                     /match_length="3.4"
                     /fragment="True"
                     /other="CDS"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a
                     href=""urn:local:.:mv-hiwsv0x"">pJC007_A909_CovR_398</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a
                     href=""urn:local:.:nx-hgbd27z"">SC_pJC005_CovR_CompleteKO
                     (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a
                     href=""urn:local:.:7a-hgqv127"">TH4-pJC007_ddCas12</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a
                     href=""urn:local:.:h2-hiaworf"">pJC007_A909_CovR_166</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; traI
                     (fragment) (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /label="traI (fragment)"
     primer_bind     5611..5627
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     primer_bind     5611..5627
                     /note="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     5611..5627
                     /created_by="User"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13_R
                     (<a href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13_R
                     (<a href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /label="M13_R"
     CDS             complement(5694..6317)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
                     /label="TetR"
     misc_feature    5694..5717
                     /Sequence="TTAAGACCCACTTTCACATTTAAG"
                     /Hairpin_Tm="39.7"
                     /Tm="55.3"
                     /Self_Dimer_Tm="None"
                     /%GC="33.3"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; TetR F
                     (<a href=""urn:local:.:32c-c8zthnl"">3015 Flexible
                     sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetR F (<a href=""urn:local:.:32c-c8zthnl"">3015 Flexible
                     sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetR F (<a href=""urn:local:.:32e-c8zthnn"">3015b Flex
                     sgRNA + cylE55 PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetR F (<a href=""urn:local:.:32e-c8zthnn"">3015b Flex
                     sgRNA + cylE55 PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetR F (<a href=""urn:local:.:357-c906sen"">cfbProm-eGFP
                     into 3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetR F (<a href=""urn:local:.:32d-c8zthnm"">3015b Flex
                     sgRNA Deleted Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetR F (<a href=""urn:local:.:32d-c8zthnm"">3015b Flex
                     sgRNA Deleted Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetR F (<a href=""urn:local:.:32f-c8zthnn"">3010 Flexible
                     sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetR F (<a href=""urn:local:.:32f-c8zthnn"">3010 Flexible
                     sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TetR F
                     (<a href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry
                     SHAM Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetR
                     F (<a href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP
                     dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetR
                     F (<a href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetR
                     F (<a href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP
                     dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetR
                     F (<a href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetR
                     F (<a href=""urn:local:.:61-gaahv0e"">3015b with mCherry
                     SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetR
                     F (<a href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP
                     dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetR
                     F (<a href=""urn:local:.:7i-gh0m63n"">GA Predicted
                     Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetR
                     F (<a href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetR
                     F (<a href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /label="TetR F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5757..5776
                     /Sequence="TAAGAAGGCTGGCTCTGCAC"
                     /modified_by="User"
                     /Hairpin_Tm="None"
                     /Tm="60.0"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=967
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3015 Flexible sgRNA"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:32c-c8zthnl"">3015 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:32e-c8zthnn"">3015b Flex sgRNA + cylE55
                     PS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:357-c906sen"">cfbProm-eGFP into
                     3015-CPF GA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:32d-c8zthnm"">3015b Flex sgRNA Deleted
                     Cryptic Promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:32f-c8zthnn"">3010 Flexible sgRNA</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cfb-TetR
                     Confirm R (<a href=""urn:local:.:14x-gtbfk0r"">3015b with
                     mCherry SHAM Proto 08-06-2020 (digested by BsaI) Fragment
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:j-fs5tu9o"">3010-flex + mCherry
                     Reporter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cfb-TetR Confirm R (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /label="cfb-TetR Confirm R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     5880..5899
                     /Sequence="CAATACGCAAACCGCCTCTC"
                     /Tm="59.6"
                     /%GC="55.0"
                     /Hairpin_Tm="41.8"
                     /Self_Dimer_Tm="None"
                     /created_by="gideo"
                     /X-Transferred_From="p3012 GA with TIP dCas9"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Primer R (<a href=""urn:local:.:5l-gaahs9y"">p3012 GA with
                     TIP dCas9</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3015b sham w/ TIP dCas9 RBS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pHY304
                     Primer R (<a href=""urn:local:.:13d-gl1gym0"">p3015b sham
                     w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Primer R (<a href=""urn:local:.:5k-gh0jlcx"">p3015b
                     sham w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Primer R (<a href=""urn:local:.:dh-gbasuap"">p3012
                     TIP 11 (original) GFP precursor_NEB (digested by NotI,
                     SphI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Primer R (<a href=""urn:local:.:jg-gh1eddv"">p3015b
                     sham w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Primer R (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Primer R (<a href=""urn:local:.:69-gh0jsjw"">p3015b
                     sham w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Primer R (<a href=""urn:local:.:lg-gh6glbz"">p3015b
                     sham w/ TIP dCas9 RBS - Rest of p3015b</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Primer R (<a href=""urn:local:.:my-gh6iq5f"">p3015b
                     sham w/ TIP dCas9 RBS - Rest of p3015b - p3015b sham w/
                     TIP dCas9 RBS - dCas9 +mCherry (reversed) - p3015b sham w/
                     TIP dCas9 RBS - Synthesized DNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Primer R (<a
                     href=""urn:local:.:l1-gh6g2on"">NEBuilder p3015b TIP dCas9
                     w/ RBS & aatII</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pHY304 Primer R (<a href=""urn:local:.:154-gl1mv2p"">GA
                     ORI into duel tet No ORI PRODUCT</a>)"
                     /label="pHY304 Primer R"
     misc_feature    6209..6228
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="60.0"
                     /Tm="59.9"
                     /Hairpin_Tm="35.4"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="25.7"
                     /Sequence="CTACACCTAGCTTCTGGGCG"
                     /Product_Size=410
                     /X-Transferred_From="p3012 w/GFP + PstI +BamHI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a href=""urn:local:.:79-ft1a98x"">p3012
                     w/GFP + PstI +BamHI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 R (3012 (digested by SphI, BamHI)
                     Fragment 2)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 R (<a
                     href=""urn:local:.:aq-ft1ghkf"">(No ERM) p3012 GA with GFP
                     extraction </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 R (<a
                     href=""urn:local:.:ab-ft1fro4"">p3012 GA with GFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 R (<a
                     href=""urn:local:.:l4-ftqlegx"">p3012 GA with GFP
                     extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     3009 cfb Confirm 2 R (<a
                     href=""urn:local:.:93-ft1fhm9"">3012 w/ GFP</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 GFP precursor_NEB WholePlasmid
                     PCR Linear (digested by BamHI, BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3009 cfb
                     Confirm 2 R (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a href=""urn:local:.:5t-g30fg6k"">p3012
                     TIP 16 GFP precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a
                     href=""urn:local:.:5l-fsw9gf9"">3012</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a href=""urn:local:.:vn-fvabwem"">p3012
                     GFP precursor_NEB WholePlasmid PCR Linear</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a href=""urn:local:.:3w-fv1256p"">p3012
                     GA with GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a href=""urn:local:.:4t-g0w0spy"">p3012
                     GFP precursor_NEB extraction</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3009
                     cfb Confirm 2 R (<a href=""urn:local:.:4e-guqe4cv"">p3012
                     GA with GFP - p3012 remove GFP for dCas9 GA</a>)"
                     /label="3009 cfb Confirm 2 R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     6225..6244
                     /note="Tetracycline resistance gene, reverse primer"
                     /label="Tet-R"
     promoter        <6426..6457
                     /created_by="User"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="p3015b sham w/ TIP dCas9 Crazy Dual
                     Tet No ORI"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     xyl/tet Promoter (<a
                     href=""urn:local:.:13j-gl1h1n7"">p3015b sham w/ TIP dCas9
                     Crazy Dual Tet No ORI</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GA ORI into duel tet No ORI PRODUCT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; xyl/tet
                     Promoter (<a href=""urn:local:.:154-gl1mv2p"">GA ORI into
                     duel tet No ORI PRODUCT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     xyl/tet Promoter (<a
                     href=""urn:local:.:13j-gl1h1n7"">p3015b sham w/ TIP dCas9
                     Crazy Dual Tet No ORI</a>)"
                     /label="xyl/tet Promoter"
     protein_bind    6427..6445
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    6427..6445
                     /note="bacterial operator O<sub>1</sub> for the
                     <i>tetR</i> and <i>tetA</i> genes"
                     /bound_moiety="tetracycline repressor TetR"
                     /X-Transferred_From="CR_COH1_KO_Cassette_Colony_3"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet operator (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tet operator (<a
                     href=""urn:local:.:ra-gj1983i"">pJC005.em</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a href=""urn:local:.:14i-gtbe5tv"">pJC007 Sham
                     Knockdown Plasmid (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a href=""urn:local:.:s0-grvqn0c"">pJC007 Sham
                     Knockdown Plasmid</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a href=""urn:local:.:so-grvreud"">pJC007
                     (digested by BtgZI) Fragment 2 - CylE Knockdown Cassette
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a href=""urn:local:.:f7-gqwayv7"">pJC007 tet
                     promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a href=""urn:local:.:29-go6k343"">pJC007
                     (digested by BtgZI) Fragment 2</a>)"
                     /label="tet operator"
     protein_bind    complement(6427..6445)
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pRMC2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tet
                     operator (<a href=""urn:local:.:2m-fs5xmi4"">pRMC2</a>)"
                     /label="tet operator"
     misc_difference 6429..6444
                     /created_by="gideo"
                     /modified_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 TIP 16 GFP precursor_NEB Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TetO (2)
                     (<a href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:dh-gbasuap"">p3012 TIP 11
                     (original) GFP precursor_NEB (digested by NotI, SphI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:2l-g3053xu"">p3012 GFP
                     precursor_NEB (digested by XmaI, SphI) Fragment 1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:c8-gbabrez"">p3012 TIP 11
                     (original) GFP precursor_NEB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a
                     href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:6v-g30htzc"">p3012 TIP 16 GFP
                     precursor_NEB Copy (digested by XmaI, SphI) Fragment
                     1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:6h-g30hrxp"">p3012 TIP 14 GFP
                     precursor_NEB Copy (digested by XmaI, SphI) Fragment
                     1</a>)"
                     /label="TetO (2)"
     misc_feature    6429..6444
                     /created_by="gideo"
                     /modified_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p3012 TIP 16 GFP precursor_NEB Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TetO (1)
                     (<a href=""urn:local:.:5t-g30fg6k"">p3012 TIP 16 GFP
                     precursor_NEB Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a
                     href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:dh-gbasuap"">p3012 TIP 11
                     (original) GFP precursor_NEB (digested by NotI, SphI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:2l-g3053xu"">p3012 GFP
                     precursor_NEB (digested by XmaI, SphI) Fragment 1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:c8-gbabrez"">p3012 TIP 11
                     (original) GFP precursor_NEB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a
                     href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:6v-g30htzc"">p3012 TIP 16 GFP
                     precursor_NEB Copy (digested by XmaI, SphI) Fragment
                     1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:6h-g30hrxp"">p3012 TIP 14 GFP
                     precursor_NEB Copy (digested by XmaI, SphI) Fragment
                     1</a>)"
                     /label="TetO (1)"
     CDS             6429..6444
                     /created_by="gideo"
                     /X-Transferred_From="pDCcompEV_promoter p3015"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetO (1) (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDCcompHssS_promoter p3015"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TetO (1)
                     (<a href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:gh-fv5jgkb"">p3012 GA with
                     GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:m3-fv6z0n5"">p3012 GFP
                     precursor_NEB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:ol-fv79v4s"">p3012 GFP
                     precursor_NEB (-4bp b4 tetO-2) (digested by BsaI, BamHI)
                     Fragment 1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:kb-fv5qpjw"">p3012 GFP
                     precursor_NEB (-4bp b4 tetO-2)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a
                     href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (1) (<a href=""urn:local:.:4z-fv138oo"">p3012 GA with GFP
                     Alt1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:wi-fvacily"">p3012 GFP
                     precursor_NEB WholePlasmid PCR Linear (digested by BamHI,
                     BsaI) Fragment 2</a>)"
                     /label="TetO (1)"
     CDS             6429..6444
                     /created_by="gideo"
                     /X-Transferred_From="pDCcompEV_promoter p3015"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     TetO (2) (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDCcompHssS_promoter p3015"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; TetO (2)
                     (<a href=""urn:local:.:bh-hg5zdzb"">pDCcompHssS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:gh-fv5jgkb"">p3012 GA with
                     GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a
                     href=""urn:local:.:ax-hg5ys1m"">pDCcompHssRS_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:9c-hg5va0n"">pDCcompEV_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a
                     href=""urn:local:.:dz-hg63uzt"">pDCHemeGFP_promoter
                     p3015</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TetO
                     (2) (<a href=""urn:local:.:4z-fv138oo"">p3012 GA with GFP
                     Alt1</a>)"
                     /label="TetO (2)"
     CDS             6510..10430
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARN
                     DHYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATY
                     RNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLR
                     SFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSL
                     REHFENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKG
                     LNEVLNLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFC
                     KYKTLLRNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYER
                     RISELTGKITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAAL
                     DQPLPTTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEME
                     PSLSFYNKARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLG
                     IMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTT
                     PILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRD
                     FLSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKL
                     YLFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRM
                     AHRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSH
                     EIIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNL
                     IYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYL
                     SQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKD
                     YPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTI
                     KNHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQ
                     FDAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLL
                     ENDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDA
                     DANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN"
                     /label="AsCpf1"
     primer_bind     complement(9216..9238)
                     /Sequence="CCTCGGTCTATGCCAATAATTGG"
                     /Tm="59.2"
                     /%GC="47.8"
                     /Hairpin_Tm="53.6"
                     /Self_Dimer_Tm="None"
                     /created_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC007 (digested by BtgZI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:29-go6k343"">pJC007 (digested by BtgZI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:dl-gqat620"">pJC007 (digested by BtgZI)
                     Fragment 2 2 - CylE Knockdown Cassette </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:x-go6f7ev"">pJC007</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:d2-gqat0if"">pJC007 (digested by BtgZI)
                     Fragment 2 2</a>)"
                     /label
     primer_bind     9226..9244
                     /created_by="gideo"
                     /Sequence="GCATAGACCGAGGAGAACG"
                     /Tm="57.4"
                     /%GC="57.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC007 (digested by BtgZI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:29-go6k343"">pJC007 (digested by BtgZI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:dl-gqat620"">pJC007 (digested by BtgZI)
                     Fragment 2 2 - CylE Knockdown Cassette </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:x-go6f7ev"">pJC007</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:d2-gqat0if"">pJC007 (digested by BtgZI)
                     Fragment 2 2</a>)"
                     /label
     primer_bind     complement(9582..9604)
                     /Sequence="GGATAATCTTTCAGAACCAGGCA"
                     /Tm="58.5"
                     /%GC="43.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC007 Double dCas12 mutations"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; dCas12
                     Mutations Seq R (pJC007 Double dCas12 mutations)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     dCas12 Mutations Seq R (<a
                     href=""urn:local:.:v5-gz1iqqn"">pJC007 Double dCas12
                     mutations</a>)"
                     /label="dCas12 Mutations Seq R"
     misc_feature    10449..10476
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="49.1"
                     /fragment="True"
                     /other="misc_feature"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:mv-hiwsv0x"">pJC007_A909_CovR_398</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:nx-hgbd27z"">SC_pJC005_CovR_CompleteKO
                     (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:7a-hgqv127"">TH4-pJC007_ddCas12</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:h2-hiaworf"">pJC007_A909_CovR_166</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (fragment) (<a
                     href=""urn:local:.:fq-hgb5znw"">pJC007_CovR166</a>)"
                     /label="MCS (fragment)"
     primer_bind     10588..10622
                     /Sequence="GAGAAATCCCTAAATAAAAAGATGCCAGTGTGCTG"
                     /Tm="66.3"
                     /%GC="40.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="5.4"
                     /created_by="gideo"
                     /X-Transferred_From="CR_COH1_KO_Cassette_Colony_3"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SOE PCR Universal Fwd (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SOE PCR Universal Fwd (<a
                     href=""urn:local:.:ra-gj1983i"">pJC005.em</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SOE PCR
                     Universal Fwd (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (pJC007 Double dCas12 mutations)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (<a
                     href=""urn:local:.:7f-hgqv7f8"">pJC005_CydDC-CAT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (<a
                     href=""urn:local:.:x-hgbsi03"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2 - Annealed oligo
                     product Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (<a
                     href=""urn:local:.:jw-hgb9zs5"">pJC005_CydDC-CAT_3piece
                     (digested by Esp3I) Fragment 2 - Annealed oligo product
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Fwd (<a
                     href=""urn:local:.:h6-hiawori"">pJC007_A909_CovR_398</a>)"
                     /label="SOE PCR Universal Fwd"
     misc_feature    10597..10785
                     /label="pJC005 Sham Target Cassette (concatenated sequence
                     2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        10597..10616
                     /X-Transferred_From="CR_COH1_KO_Cassette_Colony_3"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:cp-hcb1yvp"">pJC005 KO
                     CydDC Chloro Exchange</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:9o-gpba3j5"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:17z-gmr0hlg"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:xn-gkqfp2u"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:a7-hcavy3x"">pJC005 Sham
                     Target</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:a7-hcavy3x"">pJC005 Sham
                     Target</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:yh-gsq9yx0"">pJC005 Sham
                     Target Cassette</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:yh-gsq9yx0"">pJC005 Sham
                     Target Cassette</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Ligation
                     (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:14i-gtbe5tv"">pJC007 Sham
                     Knockdown Plasmid (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:14i-gtbe5tv"">pJC007 Sham
                     Knockdown Plasmid (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:s0-grvqn0c"">pJC007 Sham
                     Knockdown Plasmid</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:s0-grvqn0c"">pJC007 Sham
                     Knockdown Plasmid</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:so-grvreud"">pJC007
                     (digested by BtgZI) Fragment 2 - CylE Knockdown Cassette
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:so-grvreud"">pJC007
                     (digested by BtgZI) Fragment 2 - CylE Knockdown Cassette
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /label="Ligation"
     misc_difference 10597..10616
                     /created_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005.em (digested by BtgZI) Fragment
                     2 -  CylE KO Construct for insert into pJC005"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Homology
                     to 5' end of KO Insert (<a
                     href=""urn:local:.:17z-gmr0hlg"">pJC005.em (digested by
                     BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Homology to 5' end of KO Insert (<a
                     href=""urn:local:.:r7-gj1943h"">pJC005.em</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Homology to 5' end of KO Insert (<a
                     href=""urn:local:.:tw-gk13p5q"">pJC005.em (digested by
                     BtgZI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Homology to 5' end of KO Insert (<a
                     href=""urn:local:.:xn-gkqfp2u"">pJC005.em (digested by
                     BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Homology to pJC005 US BtgZI site (<a
                     href=""urn:local:.:vd-gkq913r""> CylE KO Construct for
                     insert into pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Homology to pJC005 US BtgZI site (<a
                     href=""urn:local:.:17z-gmr0hlg"">pJC005.em (digested by
                     BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Homology to pJC005 US BtgZI site (<a
                     href=""urn:local:.:x6-gkqfnmq""> CylE KO Construct for
                     insert into pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Homology to pJC005 US BtgZI site (<a
                     href=""urn:local:.:xn-gkqfp2u"">pJC005.em (digested by
                     BtgZI) Fragment 2 -  CylE KO Construct for insert into
                     pJC005</a>)"
                     /label="Homology to 5' end of KO Insert"
     primer_bind     10603..10622
                     /Tm=56
                     /Sequence="AAAAAGATGCCAGTGTGCTG"
                     /Description="<html><body></body></html>"
                     /label="oJC270"
     promoter        10639..10690
                     /label="P3"
     misc_feature    10691..10709
                     /label="repete"
     source          <10705..>10737
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /plasmid="New Assembly"
                     /X-Transferred_From="pJC005 KO CydDC Chloro Exchange"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; source
                     synthetic DNA construct (<a
                     href=""urn:local:.:cp-hcb1yvp"">pJC005 KO CydDC Chloro
                     Exchange</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-11746/588; source Cloning vector
                     pDC123 (<a href=""urn:local:.:a6-hcavy3x"">pDC123
                     (modified) - Chlro Resistane Cassette</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-11746/588; source Cloning vector
                     pDC123 (<a href=""urn:local:.:9z-hcavv3m"">pDC123
                     (modified) - Chlro Resistane Cassette</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-11746/588; source Cloning vector
                     pDC123 (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%; source synthetic DNA
                     construct (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%; source synthetic DNA
                     construct (<a href=""urn:local:.:9o-gpba3j5"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; source synthetic DNA
                     construct (<a href=""urn:local:.:17z-gmr0hlg"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; source synthetic DNA
                     construct (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%; source synthetic DNA
                     construct (<a href=""urn:local:.:xn-gkqfp2u"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece (digested by
                     Esp3I) Fragment 1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; source
                     synthetic DNA construct (<a
                     href=""urn:local:.:ie-hgb88zm"">pJC005_CydDC-CAT_3piece
                     (digested by Esp3I) Fragment 1</a>)"
                     /label="source synthetic DNA construct"
     note            10710..10732
                     /created_by="gideo"
                     /label="Sham Protospacer"
     note            10710..10715
                     /created_by="gideo"
                     /modified_by="gideo"
                     /label="Esp3I Cut Sites to remove sham protospacer"
     misc_feature    <10716..>10726
                     /created_by="User"
                     /modified_by="hoovet01"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pJC005 KO CydDC Chloro Exchange"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:cp-hcb1yvp"">pJC005 KO CydDC Chloro
                     Exchange</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:a7-hcavy3x"">pJC005 Sham Target</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:yh-gsq9yx0"">pJC005 Sham Target
                     Cassette</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:1en-h0r49oq"">pJC005 Sham Target</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:s0-grvqn0c"">pJC007 Sham Knockdown
                     Plasmid</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:cp-hcb1yvp"">pJC005 KO CydDC Chloro
                     Exchange</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:a7-hcavy3x"">pJC005 Sham Target</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:ie-hgb88zm"">pJC005_CydDC-CAT_3piece
                     (digested by Esp3I) Fragment 1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:xl-gsq8pwc"">pJC007 (digested by BtgZI)
                     Fragment 2 - CylE Knockdown Cassette TAHed1 extraction
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:y7-gsq9n27"">pJC007 Sham Knockdown
                     Cassette</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:1en-h0r49oq"">pJC005 Sham Target</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Protospacer Sequence (<a
                     href=""urn:local:.:yh-gsq9yx0"">pJC005 Sham Target
                     Cassette</a>)"
                     /label="Protospacer Sequence"
     note            10727..10732
                     /created_by="gideo"
                     /modified_by="gideo"
                     /label="Esp3I Cut Sites to remove sham protospacer"
     misc_feature    10733..10751
                     /label="repete"
     misc_feature    10734..10708
                     /Source="referenceToDocument: urn:local:.:na-hgbd27l"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005 Editable Sham (digested by
                     Esp3I) Fragment 2 - Annealed oligo product"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pJC005
                     Editable Sham (digested by Esp3I) Fragment 2 (concatenated
                     sequence 1) (<a href=""urn:local:.:ou-hgbd6fw"">pJC005
                     Editable Sham (digested by Esp3I) Fragment 2 - Annealed
                     oligo product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005 Editable Sham (digested by Esp3I) Fragment 2
                     (concatenated sequence 1) (<a
                     href=""urn:local:.:jw-hiqj71t"">pJC005 Editable Sham
                     (digested by Esp3I) Fragment 2 - Annealed oligo product
                     3</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:31/-92;
                     pJC005.em (digested by BtgZI) Fragment 2 (concatenated
                     sequence 1) (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:31/-92;
                     pJC005.em (digested by BtgZI) Fragment 2 (concatenated
                     sequence 1) (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:31/-92;
                     pJC005.em (digested by BtgZI) Fragment 2 (concatenated
                     sequence 1) (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:31/-92;
                     pJC005.em (digested by BtgZI) Fragment 2 (concatenated
                     sequence 1) (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:31/-92;
                     pJC005.em (digested by BtgZI) Fragment 2 (concatenated
                     sequence 1) (<a href=""urn:local:.:9o-gpba3j5"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:31/-92;
                     pJC005.em (digested by BtgZI) Fragment 2 (concatenated
                     sequence 1) (<a href=""urn:local:.:xn-gkqfp2u"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:31/-92;
                     pJC005.em (digested by BtgZI) Fragment 2 (concatenated
                     sequence 1) (<a
                     href=""urn:local:.:7f-hgqv7f8"">pJC005_CydDC-CAT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:31/-92;
                     pJC005.em (digested by BtgZI) Fragment 2 (concatenated
                     sequence 1) (<a
                     href=""urn:local:.:x-hgbsi03"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2 - Annealed oligo
                     product Copy</a>)"
                     /label="pJC005 Editable Sham (digested by Esp3I) Fragment
                     2 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     note            10752..10757
                     /created_by="gideo"
                     /label="XhoI Site to add homology arms"
     primer_bind     complement(10752..10786)
                     /Sequence="GCCAGTGCCAAGCTTGCATGTCTGCAGGCCTCGAG"
                     /Tm="77.3"
                     /%GC="62.9"
                     /Hairpin_Tm="65.4"
                     /Self_Dimer_Tm="26.6"
                     /created_by="gideo"
                     /X-Transferred_From="pJC005.em"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Rev (<a
                     href=""urn:local:.:ra-gj1983i"">pJC005.em</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC007 Double dCas12 mutations"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SOE PCR
                     Universal Rev (pJC007 Double dCas12 mutations)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Rev (<a
                     href=""urn:local:.:h6-hiawori"">pJC007_A909_CovR_398</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Rev (<a
                     href=""urn:local:.:v5-gz1iqqn"">pJC007 Double dCas12
                     mutations</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Rev (<a
                     href=""urn:local:.:x-go6f7ev"">pJC007</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SOE
                     PCR Universal Rev (<a
                     href=""urn:local:.:ra-gj1983i"">pJC005.em</a>)"
                     /label="SOE PCR Universal Rev"
     gene            10757..10752
                     /note="PJC005 CydDC"
                     /X-Transferred_From="pJC005 Editing CydDC"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; PJC005
                     CydDC gene (pJC005 Editing CydDC)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PJC005 CydDC gene (<a
                     href=""urn:local:.:1ey-h0r5bz4"">pJC005 Editing
                     CydDC</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005 CydDC PS gene (<a
                     href=""urn:local:.:cp-hcb1yvp"">pJC005 KO CydDC Chloro
                     Exchange</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-13762/-3373; ermAM gene (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-13762/-3373; ermAM gene (<a
                     href=""urn:local:.:1dk-h0qw6u1"">pORI23 (based on
                     high-quality MCS sequencing data)</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-13762/-3373; ermAM gene (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-2313/8514; repD gene (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-2313/8514; repD gene (<a
                     href=""urn:local:.:1dk-h0qw6u1"">pORI23 (based on
                     high-quality MCS sequencing data)</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-2313/8514; repD gene (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; PJC005
                     CydDC gene (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PJC005 CydDC gene (<a
                     href=""urn:local:.:1ey-h0r5bz4"">pJC005 Editing
                     CydDC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PJC005 CydDC gene (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PJC005 CydDC gene (pJC005 Editing CydDC)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005 CydDC PS gene (<a
                     href=""urn:local:.:cp-hcb1yvp"">pJC005 KO CydDC Chloro
                     Exchange</a>)"
                     /Alternative_Match="100.00%; PJC005 CydDC gene (<a
                     href=""urn:local:.:jw-hgb9zs5"">pJC005_CydDC-CAT_3piece
                     (digested by Esp3I) Fragment 2 - Annealed oligo product
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:29/-4999;
                     pJC005 Backbone gene (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%;
                     Adjusted-Interval-0:-10531/-20920; ermAM gene (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%;
                     Adjusted-Interval-0:-10531/-20920; ermAM gene (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%;
                     Adjusted-Interval-0:-10531/-20920; ermAM gene (<a
                     href=""urn:local:.:1dk-h0qw6u1"">pORI23 (based on
                     high-quality MCS sequencing data)</a>)"
                     /label="PJC005 CydDC gene"
     ligation        10765..10785
                     /X-Transferred_From="CR_COH1_KO_Cassette_Colony_3"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:cp-hcb1yvp"">pJC005 KO
                     CydDC Chloro Exchange</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:9o-gpba3j5"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:17z-gmr0hlg"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:xn-gkqfp2u"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:a7-hcavy3x"">pJC005 Sham
                     Target</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:a7-hcavy3x"">pJC005 Sham
                     Target</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:yh-gsq9yx0"">pJC005 Sham
                     Target Cassette</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:yh-gsq9yx0"">pJC005 Sham
                     Target Cassette</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Ligation
                     (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:14i-gtbe5tv"">pJC007 Sham
                     Knockdown Plasmid (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:14i-gtbe5tv"">pJC007 Sham
                     Knockdown Plasmid (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:s0-grvqn0c"">pJC007 Sham
                     Knockdown Plasmid</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:s0-grvqn0c"">pJC007 Sham
                     Knockdown Plasmid</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:so-grvreud"">pJC007
                     (digested by BtgZI) Fragment 2 - CylE Knockdown Cassette
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a href=""urn:local:.:so-grvreud"">pJC007
                     (digested by BtgZI) Fragment 2 - CylE Knockdown Cassette
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /label="Ligation"
     primer_bind     complement(10766..10785)
                     /Tm=61
                     /Sequence="CCAGTGCCAAGCTTGCATGT"
                     /Description="<html><body></body></html>"
                     /label="oJC271"
     misc_difference 10767..10785
                     /created_by="gideo"
                     /modified_by="gideo"
                     /label="Homology to pJC005 DS of BtgZI Site"
     CDS             10780..10934
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacZα
                     (fragment) (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:mv-hiwsv0x"">pJC007_A909_CovR_398</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:nx-hgbd27z"">SC_pJC005_CovR_CompleteKO
                     (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:7a-hgqv127"">TH4-pJC007_ddCas12</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:h2-hiaworf"">pJC007_A909_CovR_166</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:fq-hgb5znw"">pJC007_CovR166</a>)"
                     /label="lacZα (fragment)"
     primer_bind     10780..10800
                     /Sequence="CACTGGCCGTCGTTTTACAAC"
                     /Tm="60.3"
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="hoovet01"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pBSU101 cfb-promoter eGFP &
                     downstream"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pKD101
                     F (<a href=""urn:local:.:338-c8ztrii"">pBSU101
                     cfb-promoter eGFP & downstream</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:343-c8ztwko"">pKD101 PCR
                     Product</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pKD101 F
                     (<a href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry
                     SHAM Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:13d-gl1gym0"">p3015b sham
                     w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:h-eq0qjpb"">3015b with
                     mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:5k-gh0jlcx"">p3015b sham
                     w/ TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:61-gaahv0e"">3015b with
                     mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:jg-gh1eddv"">p3015b sham
                     w/ TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:7i-gh0m63n"">GA Predicted
                     Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:69-gh0jsjw"">p3015b sham
                     w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:lg-gh6glbz"">p3015b sham
                     w/ TIP dCas9 RBS - Rest of p3015b</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pKD101 F (<a href=""urn:local:.:my-gh6iq5f"">p3015b sham
                     w/ TIP dCas9 RBS - Rest of p3015b - p3015b sham w/ TIP
                     dCas9 RBS - dCas9 +mCherry (reversed) - p3015b sham w/ TIP
                     dCas9 RBS - Synthesized DNA</a>)"
                     /label="pKD101 F"
     misc_feature    complement(10781..10797)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCR2.1-TOPO (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:33c-c8ztrwg"">pCR2.1-TOPO
                     (modified)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:lg-gh6glbz"">p3015b sham w/ TIP dCas9
                     RBS - Rest of p3015b</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_forward20_primer (<a
                     href=""urn:local:.:my-gh6iq5f"">p3015b sham w/ TIP dCas9
                     RBS - Rest of p3015b - p3015b sham w/ TIP dCas9 RBS -
                     dCas9 +mCherry (reversed) - p3015b sham w/ TIP dCas9 RBS -
                     Synthesized DNA</a>)"
                     /label="M13_forward20_primer"
     primer_bind     complement(10781..10798)
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /label="M13 Forward"
     primer_bind     complement(10781..10797)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /X-Transferred_From="CR_COH1_KO_Cassette_Colony_3"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:cp-hcb1yvp"">pJC005 KO
                     CydDC Chloro Exchange</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:9o-gpba3j5"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:9m-gpba3j5"">pJC005
                     Original.em</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:tw-gk13p5q"">pJC005.em
                     (digested by BtgZI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:17z-gmr0hlg"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a
                     href=""urn:local:.:ra-gj1983i"">pJC005.em</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:xn-gkqfp2u"">pJC005.em
                     (digested by BtgZI) Fragment 2 -  CylE KO Construct for
                     insert into pJC005</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:a7-hcavy3x"">pJC005 Sham
                     Target</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 fwd
                     (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:14i-gtbe5tv"">pJC007 Sham
                     Knockdown Plasmid (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:s0-grvqn0c"">pJC007 Sham
                     Knockdown Plasmid</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:so-grvreud"">pJC007 (digested
                     by BtgZI) Fragment 2 - CylE Knockdown Cassette </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:9m-gpba3j5"">pJC005
                     Original.em</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:g-eq5azla"">pffluc_Kan</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:r7-gj1943h"">pJC005.em</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a
                     href=""urn:local:.:85-fs11jnf"">pAT28-proD/SaEGFP</a>)"
                     /label="M13 fwd"
     primer_bind     complement(10781..10797)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="52.9"
                     /Tm="54.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTAAAACGACGGCCAGT"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pABC_ffluc_Erm"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13-F
                     (-20) (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /label="M13-F (-20)"
     note            10786..5753
                     /created_by="gideo"
                     /modified_by="gideo"
                     /label="pJC005 empty Backbone"
     note            10786..4172
                     /created_by="gideo"
                     /label="Piece1"
     primer_bind     10788..10806
                     /Sequence="GTCGTTTTACAACGTCGTG"
                     /Tm="55.2"
                     /%GC="47.4"
                     /Hairpin_Tm="52.0"
                     /Self_Dimer_Tm="13.4"
                     /created_by="gideo"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry Placeholder"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Fwd Bind
                     (<a href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Fwd
                     Bind (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /label="Fwd Bind"
     misc_feature    complement(10790..10812)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCR2.1-TOPO (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a
                     href=""urn:local:.:33c-c8ztrwg"">pCR2.1-TOPO
                     (modified)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a
                     href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry SHAM
                     Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a href=""urn:local:.:7i-gh0m63n"">GA
                     Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13_pUC_fwd_primer (<a
                     href=""urn:local:.:lg-gh6glbz"">p3015b sham w/ TIP dCas9
                     RBS - Rest of p3015b</a>)"
                     /label="M13_pUC_fwd_primer"
     primer_bind     complement(10790..10812)
                     /note="In lacZ gene"
                     /label="M13/pUC Forward"
     misc_feature    complement(10791..10810)
                     /created_by="primer3"
                     /%GC="45.0"
                     /Tm="55.3"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="7.6"
                     /Sequence="CAGTCACGACGTTGTAAAAC"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pBSU101 cfb-promoter mCherry &
                     downstream"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 1,219
                     R (<a href=""urn:local:.:35m-c94fcjg"">pBSU101
                     cfb-promoter mCherry & downstream</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 1,219 R
                     (<a href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry
                     SHAM Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,219 R (<a href=""urn:local:.:13d-gl1gym0"">p3015b sham
                     w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,219 R (<a href=""urn:local:.:h-eq0qjpb"">3015b with
                     mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,219 R (<a href=""urn:local:.:1g-fjr13zh"">pBSU101
                     cfb-promoter mCherry & downstream</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,219 R (<a href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/
                     TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,219 R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,219 R (<a href=""urn:local:.:61-gaahv0e"">3015b with
                     mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,219 R (<a href=""urn:local:.:jg-gh1eddv"">p3015b sham w/
                     TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,219 R (<a href=""urn:local:.:7i-gh0m63n"">GA Predicted
                     Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,219 R (<a href=""urn:local:.:69-gh0jsjw"">p3015b sham w/
                     TIP dCas9 RBS</a>)"
                     /label="1,219 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10796..10815)
                     /Sequence="TTTCCCAGTCACGACGTTGT"
                     /Tm="59.8"
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="1.5"
                     /created_by="hoovet01"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pKD101 PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:343-c8ztwko"">pKD101 PCR Product</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBSU101
                     MCS Universal R (<a href=""urn:local:.:14x-gtbfk0r"">3015b
                     with mCherry SHAM Proto 08-06-2020 (digested by BsaI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:13d-gl1gym0"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:h-eq0qjpb"">3015b with mCherry
                     Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/ TIP dCas9
                     REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:61-gaahv0e"">3015b with mCherry SHAM
                     Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:jg-gh1eddv"">p3015b sham w/ TIP dCas9
                     RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:7i-gh0m63n"">GA Predicted Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:69-gh0jsjw"">p3015b sham w/ TIP dCas9
                     RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS Universal R (<a
                     href=""urn:local:.:lg-gh6glbz"">p3015b sham w/ TIP dCas9
                     RBS - Rest of p3015b</a>)"
                     /label="pBSU101 MCS Universal R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(10796..10815)
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="1.5"
                     /Tm="59.8"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTTCCCAGTCACGACGTTGT"
                     /created_by="primer3"
                     /Product_Size=1288
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pBSU101 cfb-promoter eGFP &
                     downstream"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 1,297
                     R (<a href=""urn:local:.:338-c8ztrii"">pBSU101
                     cfb-promoter eGFP & downstream</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3015b with mCherry SHAM Proto
                     08-06-2020 (digested by BsaI) Fragment 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 1,297 R
                     (<a href=""urn:local:.:14x-gtbfk0r"">3015b with mCherry
                     SHAM Proto 08-06-2020 (digested by BsaI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,297 R (<a href=""urn:local:.:13d-gl1gym0"">p3015b sham
                     w/ TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,297 R (<a href=""urn:local:.:h-eq0qjpb"">3015b with
                     mCherry Placeholder</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,297 R (<a href=""urn:local:.:5k-gh0jlcx"">p3015b sham w/
                     TIP dCas9 REVCOM</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,297 R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,297 R (<a href=""urn:local:.:61-gaahv0e"">3015b with
                     mCherry SHAM Proto 08-06-2020</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,297 R (<a href=""urn:local:.:jg-gh1eddv"">p3015b sham w/
                     TIP dCas9 RBS and new RS PvuI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,297 R (<a href=""urn:local:.:7i-gh0m63n"">GA Predicted
                     Product</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,297 R (<a href=""urn:local:.:69-gh0jsjw"">p3015b sham w/
                     TIP dCas9 RBS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     1,297 R (<a href=""urn:local:.:lg-gh6glbz"">p3015b sham w/
                     TIP dCas9 RBS - Rest of p3015b</a>)"
                     /label="1,297 R"
     primer_bind     complement(10796..10815)
                     /Sequence="TTTCCCAGTCACGACGTTGT"
                     /Tm="59.8"
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="1.5"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=1288
                     /modified_by="gideo"
                     /X-Transferred_From="pBSU101 cfb-promoter eGFP &
                     downstream"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS R (REAL) (<a
                     href=""urn:local:.:1e-eq0rlgc"">pBSU101 cfb-promoter eGFP
                     & downstream</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBSU101
                     MCS R (REAL) (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBSU101 MCS R (REAL) (<a
                     href=""urn:local:.:1e-eq0rlgc"">pBSU101 cfb-promoter eGFP
                     & downstream</a>)"
                     /label="pBSU101 MCS R (REAL)"
     primer_bind     complement(10796..10815)
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="1.5"
                     /Tm="59.8"
                     /Sequence="TTTCCCAGTCACGACGTTGT"
                     /created_by="primer3"
                     /X-Transferred_From="pBSU101 Universal MCS R"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1i-eq0rlgc"">pBSU101 Universal MCS
                     R</a>"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBSU101
                     Universal MCS R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1i-eq0rlgc"">pBSU101 Universal MCS
                     R</a>"
                     /label="pBSU101 Universal MCS R"
     primer_bind     complement(10801..10817)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="52.9"
                     /Tm="52.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTTTTCCCAGTCACGAC"
                     /X-Transferred_From="pABC_ffluc_Erm"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13-F
                     (-40) (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13-F
                     (-40) (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13-F (-40) (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /label="M13-F (-40)"
     primer_bind     complement(10802..10823)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="59.1"
                     /Tm="65.0"
                     /Hairpin_Tm="61.3"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCCAGGGTTTTCCCAGTCACGA"
                     /X-Transferred_From="pABC_ffluc_Erm"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13-F
                     (-46) (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13-F
                     (-46) (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13-F (-46) (<a
                     href=""urn:local:.:f-eq5azla"">pABC_ffluc_Erm</a>)"
                     /label="M13-F (-46)"
     primer_bind     complement(10821..10840)
                     /Sequence="CGATTAAGTTGGGTAACGCC"
                     /modified_by="hoovet01"
                     /Tm="56.9"
                     /%GC="50.0"
                     /Hairpin_Tm="41.7"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="3.7"
                     /Product_Size=2503
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pffluc_Kan"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pFfluc
                     Cloning Site US (<a
                     href=""urn:local:.:g-eq5azla"">pffluc_Kan</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pFfluc Cloning Site US (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /label="pFfluc Cloning Site US"
     primer_bind     complement(10872..10890)
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="57.9"
                     /Tm="57.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGGCCTCTTCGCTATTACG"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; PCAM45
                     Rebuild F (<a href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PCAM45 Rebuild F (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; PCAM45
                     Rebuild F (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PCAM45 Rebuild F (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PCAM45 Rebuild F (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /label="PCAM45 Rebuild F"
     misc_feature    10876..10908
                     /Mismatches=0
                     /created_by="primer3"
                     /%GC="66.7"
                     /Tm="77.9"
                     /Hairpin_Tm="49.0"
                     /Self_Dimer_Tm="24.0"
                     /Sequence="ATAGCGAAGAGGCCCGCACCGATCGCCCTTCCC"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; PCAM46
                     with OL R (<a href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PCAM46 with OL R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; PCAM46
                     with OL R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PCAM46 with OL R (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PCAM46 with OL R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /label="PCAM46 with OL R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     10876..10908
                     /created_by="primer3"
                     /%GC="66.7"
                     /Tm="77.9"
                     /Hairpin_Tm="49.0"
                     /Self_Dimer_Tm="24.0"
                     /Sequence="ATAGCGAAGAGGCCCGCACCGATCGCCCTTCCC"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; PCAM46
                     with OL R (<a href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PCAM46 with OL R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; PCAM46
                     with OL R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PCAM46 with OL R (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PCAM46 with OL R (<a href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /label="PCAM46 with OL R"
     primer_bind     complement(10911..10929)
                     /created_by="primer3"
                     /%GC="57.9"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=1447
                     /Sequence="CATTCAGGCTGCGCAACTG"
                     /modified_by="hoovet01"
                     /X-Transferred_From="pSBMC (pMBsacB-mCherry)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; 247 F;
                     247 F; pMBsacB MCS R; pMBsacB MCS R (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 247 F;
                     247 F; pMBsacB MCS R; pMBsacB MCS R (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 247
                     F; 247 F; pMBsacB MCS R; pMBsacB MCS R (<a
                     href=""urn:local:.:10-eq0r5sk"">pSBMC
                     (pMBsacB-mCherry)</a>)"
                     /label="247 F; 247 F; pMBsacB MCS R; pMBsacB MCS R"
     primer_bind     complement(10911..10929)
                     /Sequence="CATTCAGGCTGCGCAACTG"
                     /Tm="60.2"
                     /%GC="57.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=1447
                     /modified_by="hoovet01"
                     /X-Transferred_From="pMBsacB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pMBsacB MCS R (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pMBsacB
                     MCS R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pMBsacB MCS R (<a
                     href=""urn:local:.:z-eq0r5sk"">pMBsacB</a>)"
                     /label="pMBsacB MCS R"
     primer_bind     complement(11023..11044)
                     /created_by="gideo"
                     /Sequence="GCTATCTTCGTCATAGTTACCT"
                     /Tm="54.9"
                     /%GC="40.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="gideo"
                     /label="pJC005/7 PS Screen Rev (use with Fwd PS oligo to
                     screen)"
     primer_bind     complement(11023..11044)
                     /Sequence="GCTATCTTCGTCATAGTTACCT"
                     /Tm="54.9"
                     /%GC="40.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="gideo"
                     /X-Transferred_From="pJC007 (digested by BtgZI) Fragment
                     2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005&7 PS Screen R (<a
                     href=""urn:local:.:29-go6k343"">pJC007 (digested by BtgZI)
                     Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005&7 PS Screen R (<a
                     href=""urn:local:.:dl-gqat620"">pJC007 (digested by BtgZI)
                     Fragment 2 2 - CylE Knockdown Cassette </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005&7 PS Screen R (<a
                     href=""urn:local:.:x-go6f7ev"">pJC007</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005&7 PS Screen R (<a
                     href=""urn:local:.:d2-gqat0if"">pJC007 (digested by BtgZI)
                     Fragment 2 2</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="New Assembly"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pJC005&7
                     PS Screen R (<a href=""urn:local:.:ma-hiwrh9c"">New
                     Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005&7 PS Screen R (<a
                     href=""urn:local:.:29-go6k343"">pJC007 (digested by BtgZI)
                     Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005&7 PS Screen R (<a
                     href=""urn:local:.:dl-gqat620"">pJC007 (digested by BtgZI)
                     Fragment 2 2 - CylE Knockdown Cassette </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005&7 PS Screen R (<a
                     href=""urn:local:.:x-go6f7ev"">pJC007</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005&7 PS Screen R (<a
                     href=""urn:local:.:d2-gqat0if"">pJC007 (digested by BtgZI)
                     Fragment 2 2</a>)"
                     /label="pJC005&7 PS Screen R"
     primer_bind     complement(11023..11044)
                     /Sequence="GCTATCTTCGTCATAGTTACCT"
                     /Tm="54.9"
                     /%GC="40.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="gideo"
                     /X-Transferred_From="CR_COH1_KO_Cassette_Colony_3"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:cp-hcb1yvp"">pJC005 KO CydDC Chloro
                     Exchange</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:4m-hdb0mcs"">pJC005_CydDC-CAT</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:a7-hcavy3x"">pJC005 Sham Target</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:1en-h0r49oq"">pJC005 Sham Target</a>)"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pJC005_CydDC-CAT_3piece"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pJC005/7
                     PS Screen Rev (<a
                     href=""urn:local:.:ec-hgb44gx"">pJC005_CydDC-CAT_3piece</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:gk-hiavvx6"">pJC005_COH_CovR_148</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:mr-hgbcycj"">pJC005_CydDC-CAT_3piece
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:ma-hiwrh9c"">New Assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:pi-hgbe0rh"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:cp-hcb1yvp"">pJC005 KO CydDC Chloro
                     Exchange</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:a7-hcavy3x"">pJC005 Sham Target</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:5d-hdb39lw"">CR_COH1_KO_Cassette_Colony
                     _3</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:7f-hgqv7f8"">pJC005_CydDC-CAT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pJC005/7 PS Screen Rev (<a
                     href=""urn:local:.:x-hgbsi03"">pJC005_CydDC-CAT_3piece
                     (reversed) (digested by Esp3I) Fragment 2 - Annealed oligo
                     product Copy</a>)"
                     /label="pJC005/7 PS Screen Rev"
ORIGIN      
        1 aaaaagatat aatgggagat aagacggttc gtgttcgtgc tgacttgcac catatcataa
       61 aaatcgaaac agcaaagaat ggcggaaacg taaaagaagt tatggaaata agacttagaa
      121 gcaaacttaa gagtgtgttg atagtgcagt atcttaaaat tttgtataat aggaattgaa
      181 gttaaattag atgctaaaaa tttgtaatta agaaggagtg attacatgaa caaaaatata
      241 aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg
      301 aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg
      361 aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac
      421 ttatcgtcag aaaaattaaa actgaatact cgtgtcactt taattcacca agatattcta
      481 cagtttcaat tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta
      541 agcacacaaa ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt
      601 gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg
      661 cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa
      721 ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa
      781 tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg
      841 tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc
      901 gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa
      961 taattctatg agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga
     1021 taaattatta ggtatactac tgacagcttc caaggagcta aagaggtccc tagcgcttag
     1081 aatcgcttta ggaaacacga tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt
     1141 tggtgaaatt acctactttt ggaataatca caaatcacaa gtgattaatc acaaatcaca
     1201 agtgattaat cacttgttta ttaagatatt aaaagctata atttaaataa agcgtgaatt
     1261 ttattacaca aaaagagggg ggagaaactt ggaactagca tttagagaaa gcttaaaaaa
     1321 gatgagaggt accaaatcaa aagaaaaatt ctcccaagaa ttagaaatga gtagatcaaa
     1381 ttattcacga atagaatcag gaaaatcaga tccaaccata aaaacactag aacaaattgc
     1441 aaagttaact aactcaacgc tagtagtgga tttaatccca aatgagccaa cagaaccaga
     1501 accagaaaca gaatcagaac aagtaacatt ggatttagaa atggaagaag aaaaaagcaa
     1561 tgacttcgtg tgaataatgc gcgaaatcgt tgcttatttt ttttaaaagc ggtatactag
     1621 atataacgaa acaacgaact gaatagaaac gaaaaaagag ccatgacaca tttataaaat
     1681 gtttgacgac attttataaa tgcatagccc gataagattg ccaaaccaac gcttatcagt
     1741 tagtcagatg aactcttccc tcgtaagaag ttatttaatt aactttgttt gaagacggta
     1801 tataaccgta ctatcattat atagggaaat cagagagttt tcaagtatct aagctactga
     1861 atttaagaat tgttaagcaa tcaatcggaa atcgtttgat tgcttttttt gtattcattt
     1921 atagaaggtg gagtttgtat gaatcatgat gaatgtaaaa cttatataaa aaatagttta
     1981 ttggagataa gaaaattagc aaatatctat acactagaaa cgtttaagaa agagttagaa
     2041 aagagaaata tctacttaga aacaaaatca gataagtatt tttcttcgga gggggaagat
     2101 tatatatata agttaataga aaataacaaa ataatttatt cgattagtgg aaaaaaattg
     2161 acttataaag gaaaaaaatc tttttcaaaa catgcaatat tgaaacagtt gaatgaaaaa
     2221 gcaaaccaag ttaattaaac aacctatttt ataggattta taggaaagga gaacagctga
     2281 atgaatatcc cttttgttgt agaaactgtg cttcatgacg gcttgttaaa gtacaaattt
     2341 aaaaatagta aaattcgctc aatcactacc aagccaggta aaagcaaagg ggctattttt
     2401 gcgtatcgct caaaatcaag catgattggc ggtcgtggtg ttgttctgac ttccgaggaa
     2461 gcgattcaag aaaatcaaga tacatttaca cattggacac ccaacgttta tcgttatgga
     2521 acgtatgcag acgaaaaccg ttcatacacg aaaggacatt ctgaaaacaa tttaagacaa
     2581 atcaatacct tctttattga ttttgatatt cacacggcaa aagaaactat ttcagcaagc
     2641 gatattttaa caaccgctat tgatttaggt tttatgccta ctatgattat caaatctgat
     2701 aaaggttatc aagcatattt tgttttagaa acgccagtct atgtgacttc aaaatcagaa
     2761 tttaaatctg tcaaagcagc caaaataatt tcgcaaaata tccgagaata ttttggaaag
     2821 tctttgccag ttgatctaac gtgtaatcat tttggtattg ctcgcatacc aagaacggac
     2881 aatgtagaat tttttgatcc taattaccgt tattctttca aagaatggca agattggtct
     2941 ttcaaacaaa cagataataa gggctttact cgttcaagtc taacggtttt aagcggtaca
     3001 gaaggcaaaa aacaagtaga tgaaccctgg tttaatctct tattgcacga aacgaaattt
     3061 tcaggagaaa agggtttaat agggcgtaat aacgtcatgt ttaccctctc tttagcctac
     3121 tttagttcag gctattcaat cgaaacgtgc gaatataata tgtttgagtt taataatcga
     3181 ttagatcaac ccttagaaga aaaagaagta atcaaaattg ttagaagtgc ctattcagaa
     3241 aactatcaag gggctaatag ggaatacatt accattcttt gcaaagcttg ggtatcaagt
     3301 gatttaacca gtaaagattt atttgtccgt caagggtggt ttaaattcaa gaaaaaaaga
     3361 agcgaacgtc aacgtgttca tttgtcagaa tggaaagaag atttaatggc ttatattagc
     3421 gaaaaatcag atgtatacaa gccttattta gtgacgacca aaaaagagat tagagaagtg
     3481 ctaggcattc ctgaacggac attagataaa ttgctgaagg tactgaaggc gaatcaggaa
     3541 attttcttta agattaaacc aggaagaaat ggtggcattc aacttgctag tgttaaatca
     3601 ttgttgctat cgatcattaa agtaaaaaaa gaagaaaaag aaagctatat aaaggcgctg
     3661 acaaattctt ttgacttaga gcatacattc attcaagaga ctttaaacaa gctagcagaa
     3721 cgccctaaaa cggacacaca actcgatttg tttagctatg atacaggctg aaaataaaac
     3781 ccgcactatg ccattacatt tatatctatg atacgtgttt gttttttctt tgctgtttag
     3841 cgaatgatta gcagaaatat acagagtaag attttaatta attattaggg ggagaaggag
     3901 agagtagccc gaaaactttt agttggcttg gactgaacga agtgagggaa aggctactaa
     3961 aacgtcgagg ggcagtgaga gcgaagcgaa cacttgattt tttaattttc tatcttttat
     4021 aggtcattag agtatactta tttgtcctat aaactattta gcagcataat agatttattg
     4081 aataggtcat ttaagttgag catattagag gaggaaaatc ttggagaaat atttgaagaa
     4141 cccgattaca tggattggat tagttcttgt ggttacgtgg tttttaacta aaagtagtga
     4201 atttttgatt tttggtgtgt gtgtcttgtt gtcagtattt gctagtcaaa gtgattaaat
     4261 agaattctca tgtttgacag cttatcatcg gagctccgat gataagctgt caaacatgag
     4321 aattcccggg gatccttacg aaatcatcct gtggagctta gtaggtttag caagatggca
     4381 gcgcctaaat gtagaatgat aaaaggatta agagattaat ttccctaaaa atgataaaac
     4441 aagcgttttg aaagcgcttg tttttttggt ttgcagtcag agtagaatag aagtatcaaa
     4501 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
     4561 gctatgctgt taagaatggt tccaacgctc actcaaaggc ggtaatacgg ttatccacag
     4621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa agatgtgatc
     5461 cgtagcggta tgcgctccat caagaagagc gacttcgcgg agctggtgaa gtacatcacc
     5521 gacgagcaag gcaagaccga tcgggccccc tgcaggataa aaaaattgta gataaatttt
     5581 ataaaatagt tttatctaca atttttttat caggaaacag ctatgaccgc ggccgctgta
     5641 tccacataaa aataagaagc ctgcatttgc aggcttctta tttttatggt accttaagac
     5701 ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag
     5761 aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc
     5821 atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc
     5881 aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt
     5941 gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct
     6001 gtaggccgtg tacctaaatg tacttttgct ccatctcggt gacttagtaa agcacatcta
     6061 aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag
     6121 tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt
     6181 tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt
     6241 gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt
     6301 ttatctaatc tagacatcat taattcctcc tttttgttga cattatatca ttgatagagt
     6361 tatttgtcaa actagttttt tatttcgatg ccctggactt catgaaaaac taaaaaaaat
     6421 attgacactc tatcattgat agagtataat taaaataagc ttgatcgtag cgttaacaga
     6481 tctgagctcc tgcagtaaag gaggttttta tgacacagtt tgaaggcttc accaatctct
     6541 accaggtcag caagacgcta cgttttgagc ttatcccgca gggaaaaacc ctgaaacaca
     6601 ttcaggaaca ggggttcata gaggaagata aggcgcgtaa cgaccattat aaagaactga
     6661 agcctataat cgaccgtatt tataaaacgt acgcggatca gtgcctgcag ctggttcagc
     6721 tggattggga gaatctgtcc gcggctattg atagctatcg caaagagaag accgaggaaa
     6781 cccgtaacgc actgattgaa gagcaggcga cctatcggaa tgcgatccat gattacttca
     6841 tcggccgcac cgacaacctg accgatgcaa ttaacaaacg tcacgcagag atttacaaag
     6901 gtctgtttaa agcagagtta ttcaatggca aggttctgaa acagctgggt acggtcacca
     6961 ccaccgaaca cgaaaacgca ctgctgagga gctttgataa atttaccaca tatttcagcg
     7021 gtttctatga aaatcgtaag aatgtattta gcgccgaaga tatttccacc gcaattcctc
     7081 atcgtattgt gcaggataat tttccgaagt ttaaagaaaa ttgtcatatt tttacccgtc
     7141 tgatcaccgc ggtaccgagc ctgcgagagc attttgaaaa cgttaagaaa gccattggaa
     7201 tttttgtcag taccagcatt gaagaagtgt tttcgttccc gttctataac caactgctga
     7261 cccagaccca gattgatctg tacaatcagc tgctgggggg cataagccgc gaggcaggta
     7321 ccgaaaagat aaagggactc aatgaggtgc tgaatctggc aattcagaag aatgatgaaa
     7381 cggctcatat cattgctagc ctgccgcatc gtttcattcc cctgtttaag caaatcctga
     7441 gcgatcgcaa tacactgagc tttatcctcg aagagtttaa atcggacgaa gaagttatcc
     7501 agagcttttg caaatacaaa accctgctgc ggaacgaaaa tgtgctggag accgctgaag
     7561 cactgtttaa tgaactgaac tcgatcgacc tcacccatat ttttatatcc cacaaaaaac
     7621 tggaaaccat aagcagcgct ctgtgtgacc attgggatac cctgcgcaac gccctgtatg
     7681 aacggcgtat cagcgagctg accgggaaaa tcaccaaatc cgcaaaggaa aaagttcagc
     7741 gtagtctgaa acacgaggac atcaacctgc aagaaattat tagcgcagca ggtaaagagc
     7801 tgagcgaagc attcaaacag aaaaccagcg aaatcctgag ccatgcccat gctgcactgg
     7861 atcagccgct gccgaccacc ctgaaaaaac aggaggaaaa ggagattctg aaaagccaac
     7921 tggacagcct gctgggcctg tatcacctgc tggactggtt tgcagtcgat gagagcaacg
     7981 aggttgatcc tgagttctcc gctcgtctga ccggaatcaa gctggagatg gaaccgagtc
     8041 tgtcgtttta caataaagcg cgtaattacg cgaccaagaa accgtatagc gtggaaaaat
     8101 tcaaactgaa ctttcagatg ccgacccttg caagcggatg ggacgttaac aaagaaaaaa
     8161 acaatggggc aattctgttt gtgaaaaatg gcctctatta tctgggtatc atgccgaaac
     8221 agaaagggcg ctacaaagcc ctgtcatttg agccgaccga gaaaacctca gagggtttcg
     8281 acaagatgta ctacgattat ttcccggatg cggcaaaaat gatacccaaa tgtagcaccc
     8341 aactgaaggc agttacagcc cactttcaga cccataccac cccgatcctg ctgtcgaaca
     8401 attttataga gccgctggaa attaccaaag agatttatga tctgaataat ccggaaaagg
     8461 agcccaagaa atttcagacg gcgtatgcaa aaaagaccgg ggatcagaaa ggttatcgtg
     8521 aagcgctgtg caaatggatt gactttaccc gtgactttct gtcaaaatat accaaaacga
     8581 cgagcattga tctgagcagc ctacgtccga gcagccaata taaggatctg ggcgaatatt
     8641 acgccgaact gaatccgctg ctctaccata tttccttcca acgaatcgct gaaaaagaaa
     8701 taatggacgc cgttgaaacc ggcaaactgt atctgtttca aatctacaac aaagatttcg
     8761 ccaaaggcca tcacggtaag ccgaacctgc ataccctgta ttggaccggt ctgtttagcc
     8821 cggagaatct ggccaaaacc agcatcaagc tgaacggaca ggcagaactg ttttaccgcc
     8881 ccaaaagccg tatgaaaagg atggcacacc gcctgggcga aaaaatgctg aataagaaac
     8941 tcaaagatca gaaaacgccg ataccggata ccctttatca ggagctgtat gattatgtta
     9001 accaccggct gagccatgac ctgagcgacg aagcgcgtgc actgctgccg aacgtgatta
     9061 ccaaggaagt ctcgcatgaa attattaaag atcggcgctt caccagtgat aaatttttct
     9121 tccatgtacc gatcaccctg aattatcaag ccgcaaatag cccttccaaa tttaatcaac
     9181 gcgtgaatgc gtacctgaaa gagcatccgg agaccccaat tattggcata gaccgaggag
     9241 aacgcaatct catttatatc accgtcattg atagcaccgg taagatcctg gaacagcgta
     9301 gcctgaatac cattcagcag tttgactacc agaaaaagct ggacaacaga gaaaaggaac
     9361 gtgtagccgc ccggcaggct tggagtgtgg tgggtactat caaggatctg aagcaggggt
     9421 atctctccca agttatccat gaaattgtcg atctaatgat tcactatcaa gcagtagtgg
     9481 tactggaaaa tctgaatttc ggtttcaaaa gcaaacgtac agggatcgct gaaaaagccg
     9541 tttatcagca gttcgagaaa atgctgatag acaagctgaa ttgcctggtt ctgaaagatt
     9601 atccggcaga gaaggtgggc ggtgtgctga acccgtacca gctgactgat caatttacga
     9661 gctttgcaaa aatgggaacg cagagcggtt tcctgttcta tgttccggcg ccatatacca
     9721 gcaagataga cccgctgaca ggtttcgtag atccgtttgt ctggaaaacc attaaaaatc
     9781 atgaaagtcg caaacatttt ctggagggct ttgattttct gcactatgac gtgaaaaccg
     9841 gcgacttcat tctgcatttt aaaatgaacc gtaatctgtc ctttcagcgc ggcctgcctg
     9901 gctttatgcc ggcgtgggac attgtttttg aaaagaatga gacacagttt gatgccaaag
     9961 gtaccccctt tattgcgggg aaacgcattg tgcccgttat agaaaatcac cgcttcaccg
    10021 gacggtatag ggacttgtac ccggcaaatg aattgatagc gctgctggag gagaaaggta
    10081 ttgtctttcg ggatggatca aacatcctgc cgaagctgct ggagaacgat gacagccacg
    10141 caatagacac catggtagcg ctgatccgaa gcgtgctgca gatgcgtaac agtaatgcgg
    10201 ctacggggga agactacatt aatagcccgg tccgtgatct gaacggcgtt tgtttcgata
    10261 gcagatttca aaatccggag tggccgatgg atgccgatgc caatggagct taccatatcg
    10321 ctctcaaagg tcagctccta ctgaaccatt tgaaagaatc aaaagatctg aaactgcaga
    10381 acggcatctc gaatcaggac tggctggcct acattcaaga actgagaaac taatatgacc
    10441 atgattacga attcgagctc ggtacccggg gatcctataa gttttaataa aactttaaat
    10501 agaaaaaggc ttctctcatg agaagtcttt tttatttaaa ataaatataa aataaaatag
    10561 aggctataaa tagcctctat tttatgtgag aaatccctaa ataaaaagat gccagtgtgc
    10621 tggaattcgt ccactagtca ttctacagtt tattcttgac attgcactgt ccccctggta
    10681 taataactat aatttctact cttgtagatg agacgatcag ccgcatcgtc tcaatttcta
    10741 ctcttgtaga tctcgaggcc tgcagacatg caagcttggc actggccgtc gttttacaac
    10801 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    10861 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    10921 gcctgaatgg cgaatggcgc tagcataaaa ataagaagcc tgcatttgca ggcttcttat
    10981 ttttatggcg cgccgttctg aatccttagc taatggttca acaggtaact atgacgaaga
    11041 tagcaccctg gagcaaccag gaatgaatta ctatcccttt tatcaagaag cgcacaaaaa
    11101 gaaaaacgaa atgatacacc aatcagtgca a
//
