LOCUS       SP3_GAL4_IL35_IR       12579 bp DNA     circular SYN 09-JAN-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12579)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 23, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12579
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    96..7517
                     /label=match to pHR-CMVLacZ
                     /note="color: #a6acb3"
     primer_bind     complement(320..337)
                     /label=SP6
                     /note="color: #00ff00; direction: LEFT"
     CDS             1231..2091
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1429..2088
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      2186..2868
                     /label=ColE1 origin
                     /note="color: #808080"
     promoter        3084..3410
                     /label=SV40 - Invitrogen promoter
                     /label=SV40 - Invitrogen
                     /note="color: #0000ff"
     promoter        3229..3425
                     /label=SV40 early promoter
                     /note="color: #6495ed"
     polyA_site      5105..5123
                     /label=SV40 pA
                     /note="color: #ccff66"
     polyA_signal    complement(5138..5329)
                     /label=SV40 late polyA
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_site      5199..5330
                     /label=SV40 early poly A
                     /note="color: #80ff00"
     polyA_site      5199..5329
                     /label=SV40 early poly A
                     /label=SV40 early poly A (2)
                     /note="color: #66ff66"
     repeat_region   7518..7697
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     7673..7696
                     /label=cPPT_5'seq
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7722..7807
                     /label=Gal4
                     /note="color: #99ccff"
     misc_feature    7848..7853
                     /label=SpeI
                     /note="color: #a6acb3"
     promoter        7854..7971
                     /label=mCMV
                     /note="color: #ffffff"
     primer_bind     7885..7904
                     /label=GMA_CMV.FOR
                     /note="color: black; sequence: agagctcgtttagtgaaccg"
     primer_bind     7972..8017
                     /label=Primer 1
                     /note="color: black; sequence: 
                     ctctcgacattcgttggatcgccGccATGACCCCTCAACTGCTTTT; added: 
                     2021-01-12"
     misc_feature    7992..7997
                     /label=Kozak Sequence
                     /note="color: #a6acb3"
     primer_bind     7996..8073
                     /label=(PR712) P2A-homo_IL27BSS_IL27
                     /note="color: black; sequence: 
                     TGGAGGAGAATCCAGGTCCCATGACCCCTCAACTGCTTTTGGCGCTTGTGCTCTGGGCA
                     TCTTGCCCACCTTGCTCTGGCAGAAAAGGCCCTCCCG"
     CDS             7998..9323
                     /codon_start=1
                     /label=P1108_gBlock
                     /note="color: #ffcc99"
                     /translation="MTPQLLLALVLWASCPPCSGRKGPPAALTLPRVQCRASRYPIAVD
                     CSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVL
                     NVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSL
                     KYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPAT
                     ATMSLGKGGGSGGGSGGGSGGGSRNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQT
                     LEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTS
                     FMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSET
                     VPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS"
     CDS             7998..8684
                     /codon_start=1
                     /label=EBI3
                     /note="color: #993366"
                     /translation="MTPQLLLALVLWASCPPCSGRKGPPAALTLPRVQCRASRYPIAVD
                     CSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVL
                     NVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSL
                     KYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPAT
                     ATMSLGK"
     misc_feature    7998..8057
                     /label=IL-27B Signal peptide
                     /note="UniProt- 
                     https://www.uniprot.org/blast/?about=Q14213[1-20]"
                     /note="color: #a6acb3"
     misc_feature    8058..8684
                     /label=IL-27beta
                     /note="IDT cod opt
                     https://www.uniprot.org/blast/?about=Q14213[21-229]"
                     /note="color: #ff9900"
     misc_feature    8685..8732
                     /label=(GGGS)4 linker
                     /note="IDT cod opt"
                     /note="color: #a6acb3"
     CDS             8733..9323
                     /codon_start=1
                     /label=IL12 p35
                     /note="color: #993366"
                     /translation="RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSE
                     EIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSS
                     IYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEE
                     PDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS"
     misc_feature    8733..9323
                     /label=IL-12alpha
                     /note="IDT cod opt
                     https://www.uniprot.org/blast/?about=P29459[23-219]"
                     /note="color: #ffff00"
     primer_bind     complement(9304..9344)
                     /label=(PR713) BamHI-pHR-homo_IL12a_rev
                     /note="color: black; sequence: 
                     gcggccgcaagtcaaggatcTCATGAAGCGTTCAGGTAAGACA"
     misc_feature    9327..9344
                     /label=BamHI-pHR rev homology
                     /note="color: #a6acb3"
     primer_bind     complement(9327..9344)
                     /label=Primer 2
                     /note="color: black; sequence: ggccgcaagtcaaggatc; added: 
                     2021-01-12"
     misc_feature    9345..9918
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
                     /note="color: #a6acb3"
     CDS             9937..10644
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9969..9988)
                     /label=GMA.mCh.Rev
                     /note="color: black; sequence: gcatgaactccttgatgatg"
     primer_bind     10628..10685
                     /label=p49 mCherrySTOP to AarI
                     /note="color: black; sequence: 
                     tggacgagctgtacaagTGAtgcgtgactgactga"
     misc_feature    10660..10666
                     /label=AarI
                     /note="color: #ff0080"
     misc_feature    10671..10674
                     /label=Part D
                     /note="color: #00ffff"
     primer_bind     10697..10734
                     /label=pHRUASIRESmC_SbfIhomo_pGK_fwd
                     /note="color: black; sequence: 
                     tagagtcgacctgcaCGAGGTTAACGAATTCTACCGGG"
     misc_feature    10732..11231
                     /label=promPGK
                     /note="color: #996633; direction: RIGHT"
     CDS             11252..11950
                     /codon_start=1
                     /gene="TagBFP"
                     /label=TagBFP
                     /note="color: #3366ff"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(11927..11968)
                     /label=pHR_SbfIhomo_tagBFP_rev
                     /note="color: black; sequence: 
                     gcttgcatgccTgcattaattaagcttgtgccccagtttgct"
     enhancer        11988..12579
                     /label=WPRE
                     /note="color: #a6acb3"
     misc_feature    complement(12011..12034)
                     /label=cw11
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     12042..12062
                     /label=QB0112 WPRE-R binding site
                     /note="color: #00ffff; direction: RIGHT"
ORIGIN
        1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
       61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
      301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
      361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
      421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
      481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
      901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
     6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7681 gcagagatcc agtttggatc gataagcttg atatcgaatt cggagcactg tcctccgaac
     7741 gtcggagcac tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct
     7801 ccgaacggag catgtcctcc gaacgtcgga gcactgtcct ccgaacgact agttaggcgt
     7861 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     7921 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctctcgaca
     7981 ttcgttggat cgccgccatg acccctcaac tgcttttggc gcttgtgctc tgggcatctt
     8041 gcccaccttg ctctggcaga aaaggccctc ccgccgcctt gacgttgccc cgcgtgcagt
     8101 gccgggctag tagatatccg atagctgtag attgttcatg gacattgccc ccggcaccaa
     8161 attcaacatc accggtcagt ttcatcgcta cttatcggtt gggcatggcc gcccgcggtc
     8221 atagctggcc ctgcttgcag cagaccccta caagcacgtc ctgtactatc actgatgttc
     8281 aattgtttag catggccccc tacgtattga acgtaacagc ggtacacccc tggggctcca
     8341 gcagtagctt tgtacctttt atcacggagc atattatcaa gcccgaccct ccagaaggag
     8401 ttagactgtc cccgctcgcg gagcggcagc tccaagttca atgggaacca ccgggcagct
     8461 ggccgttccc agagatattt agcttgaaat actggattcg atataaacgc caaggtgctg
     8521 ctcgctttca tcgagtaggc ccgattgagg ctacttcctt tatattgcga gcagtgagac
     8581 cgcgagctcg gtattatgtc caagttgcag cccaagatct tactgattac ggagaactct
     8641 cagattggtc cttgccagct actgcgacca tgagcctcgg taaaggaggt gggagcggcg
     8701 gcggaagtgg ggggggtagc ggtggtggct cccggaacct tccggtggca actccagatc
     8761 ctggcatgtt cccctgcttg caccattcac aaaacttgct ccgcgccgtt tcaaatatgc
     8821 ttcagaaagc ccggcaaact ttggagtttt atccatgtac ttcagaggaa attgaccatg
     8881 aggatattac taaggacaag acgtccactg tcgaagcgtg ccttcccctg gagcttacaa
     8941 aaaatgaatc ctgcctcaat tcacgcgaga caagcttcat aaccaacggt tcctgtctgg
     9001 ctagtcggaa aacatcattt atgatggcac tgtgcttgag ttccatctat gaggacctga
     9061 aaatgtacca agtcgagttt aagacaatga acgcaaaact tctgatggac cccaaaaggc
     9121 aaatctttct tgaccaaaac atgctcgcgg tgatagatga actcatgcaa gcactgaatt
     9181 tcaatagcga gactgtcccg caaaaatctt cacttgagga gccagatttc tacaagacta
     9241 agatcaaact ctgcattctt ctgcacgcgt tccgcataag agccgtgaca attgacagag
     9301 tgatgtctta cctgaacgct tcatgagatc cttgacttgc ggcccccctc tccctccccc
     9361 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     9421 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     9481 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     9541 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     9601 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     9661 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     9721 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     9781 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     9841 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     9901 tgaaaaacac gatgataaat gagcgctggc gggtccatgg tgagcaaggg cgaggaggat
     9961 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
    10021 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
    10081 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
    10141 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
    10201 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
    10261 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
    10321 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
    10381 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
    10441 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
    10501 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
    10561 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
    10621 ggcggcatgg acgagctgta caagtagggc cgcaactccc acctgcaaca tgcgtgactg
    10681 actgaggccg cgactctaga gtcgacctgc acgaggttaa cgaattctac cgggtagggg
    10741 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
    10801 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
    10861 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
    10921 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
    10981 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
    11041 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
    11101 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
    11161 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
    11221 cgggcctttc gacctgcagc ccaagcttac catgagcgag ctgattaagg agaacatgca
    11281 catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg
    11341 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
    11401 tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat
    11461 caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg
    11521 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
    11581 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg
    11641 ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc
    11701 tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct
    11761 gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc
    11821 tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta
    11881 cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca
    11941 caagcttaat taatgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
    12001 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    12061 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    12121 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    12181 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    12241 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    12301 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    12361 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    12421 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    12481 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    12541 cagacgagtc ggatctccct ttgggccgcc tccccgcat
//
