LOCUS       AL044_Gal4_activ       12423 bp DNA     circular SYN 09-JAN-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 23, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12423
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    96..7517
                     /label=match to pHR-CMVLacZ
                     /note="color: #a6acb3"
     primer_bind     complement(320..337)
                     /label=SP6
                     /note="color: #00ff00; direction: LEFT"
     CDS             1231..2091
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1429..2088
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      2186..2868
                     /label=ColE1 origin
                     /note="color: #808080"
     promoter        3084..3410
                     /label=SV40 - Invitrogen promoter
                     /label=SV40 - Invitrogen
                     /note="color: #0000ff"
     promoter        3229..3425
                     /label=SV40 early promoter
                     /note="color: #6495ed"
     polyA_site      5105..5123
                     /label=SV40 pA
                     /note="color: #ccff66"
     polyA_signal    complement(5138..5329)
                     /label=SV40 late polyA
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_site      5199..5330
                     /label=SV40 early poly A
                     /note="color: #80ff00"
     polyA_site      5199..5329
                     /label=SV40 early poly A
                     /label=SV40 early poly A (2)
                     /note="color: #66ff66"
     repeat_region   7518..7697
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     7673..7696
                     /label=cPPT_5'seq
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7722..7807
                     /label=Gal4
                     /note="color: #99ccff"
     misc_feature    7848..7853
                     /label=SpeI
                     /note="color: #a6acb3"
     promoter        7854..7971
                     /label=mCMV
                     /note="color: #ffffff"
     primer_bind     7885..7904
                     /label=GMA_CMV.FOR
                     /note="color: black; sequence: agagctcgtttagtgaaccg"
     misc_feature    7972..7991
                     /label=BamHI front
                     /note="color: #a6acb3"
     primer_bind     7977..8003
                     /label=active_TGFb.FOR
                     /note="color: black; sequence: gacattcgttggatcgccGccATGCCG"
     misc_feature    7992..7997
                     /label=Kozak Sequence
                     /note="color: #ffff00"
     CDS             7998..9170
                     /codon_start=1
                     /label=mTGFb CDS
                     /note="color: #ffff99"
                     /translation="MPPSGLRLLPLLLPLPWLLVLTPGRPAAGLSTCKTIDMELVKRKR
                     IEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYY
                     AKEVTRVLMVDRNNAIYEKTKDISHSIYMFFNTSDIREAVPEPPLLSRAELRLQRLKSS
                     VEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAH
                     SSSDSKDNKLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALD
                     TNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVL
                     ALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS"
     primer_bind     8290..8309
                     /label=TGFbeta_313.for
                     /note="color: black; sequence: AGCCCGAAGCGGACTACTAT"
     misc_feature    8664..8666
                     /label=Changed from Cys to Ser
                     /note="color: #00ccff"
     misc_feature    8670..8672
                     /label=Change from Cys to Ser
                     /note="color: #00ccff"
     primer_bind     8762..8779
                     /label=TGFbeta_783.for
                     /note="color: black; sequence: GCCCTTCCTGCTCCTCAT"
     primer_bind     complement(9152..9185)
                     /label=pHR-Gal4-mTGFb.REV
                     /note="color: black; sequence: 
                     cgcaagtcaaggatcTCAGCTGCACTTGCAGGAG"
     primer_bind     complement(9159..9185)
                     /label=active_TGFb.REV
                     /note="color: black; sequence: cgcaagtcaaggatcTCAGCTGCACTT"
     misc_feature    9171..9185
                     /label=BamHI-pHR homology
                     /note="color: #ffcc99"
     misc_feature    9189..9762
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
                     /note="color: #a6acb3"
     CDS             9781..10488
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9813..9832)
                     /label=GMA.mCh.Rev
                     /note="color: black; sequence: gcatgaactccttgatgatg"
     misc_feature    10504..10510
                     /label=AarI
                     /note="color: #ff0080"
     misc_feature    10515..10518
                     /label=Part D
                     /note="color: #00ffff"
     primer_bind     10541..10578
                     /label=pHRUASIRESmC_SbfIhomo_pGK_fwd
                     /note="color: black; sequence: 
                     tagagtcgacctgcaCGAGGTTAACGAATTCTACCGGG"
     misc_feature    10576..11075
                     /label=promPGK
                     /note="color: #996633; direction: RIGHT"
     CDS             11096..11794
                     /codon_start=1
                     /gene="TagBFP"
                     /label=TagBFP
                     /note="color: #3366ff"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(11771..11812)
                     /label=pHR_SbfIhomo_tagBFP_rev
                     /note="color: black; sequence: 
                     gcttgcatgccTgcattaattaagcttgtgccccagtttgct"
     enhancer        11832..12423
                     /label=WPRE
                     /note="color: #a6acb3"
     misc_feature    complement(11855..11878)
                     /label=cw11
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     11886..11906
                     /label=QB0112 WPRE-R binding site
                     /note="color: #00ffff; direction: RIGHT"
ORIGIN
        1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
       61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
      301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
      361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
      421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
      481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
      901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
     6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7681 gcagagatcc agtttggatc gataagcttg atatcgaatt cggagcactg tcctccgaac
     7741 gtcggagcac tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct
     7801 ccgaacggag catgtcctcc gaacgtcgga gcactgtcct ccgaacgact agttaggcgt
     7861 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     7921 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctctcgaca
     7981 ttcgttggat cgccgccatg ccgccctcgg ggctgcggct actgccgctt ctgctcccac
     8041 tcccgtggct tctagtgctg acgcccggga ggccagccgc gggactctcc acctgcaaga
     8101 ccatcgacat ggagctggtg aaacggaagc gcatcgaagc catccgtggc cagatcctgt
     8161 ccaaactaag gctcgccagt cccccaagcc agggggaggt accgcccggc ccgctgcccg
     8221 aggcggtgct cgctttgtac aacagcaccc gcgaccgggt ggcaggcgag agcgccgacc
     8281 cagagccgga gcccgaagcg gactactatg ctaaagaggt cacccgcgtg ctaatggtgg
     8341 accgcaacaa cgccatctat gagaaaacca aagacatctc acacagtata tatatgttct
     8401 tcaatacgtc agacattcgg gaagcagtgc ccgaaccccc attgctgtcc cgtgcagagc
     8461 tgcgcttgca gagattaaaa tcaagtgtgg agcaacatgt ggaactctac cagaaatata
     8521 gcaacaattc ctggcgttac cttggtaacc ggctgctgac ccccactgat acgcctgagt
     8581 ggctgtcttt tgacgtcact ggagttgtac ggcagtggct gaaccaagga gacggaatac
     8641 agggctttcg attcagcgct cactcgtctt ccgacagcaa agataacaaa ctccacgtgg
     8701 aaatcaacgg gatcagcccc aaacgtcggg gcgacctggg caccatccat gacatgaacc
     8761 ggcccttcct gctcctcatg gccacccccc tggaaagggc ccagcacctg cacagctcac
     8821 ggcaccggag agccctggat accaactatt gcttcagctc cacagagaag aactgctgtg
     8881 tgcggcagct gtacattgac tttaggaagg acctgggttg gaagtggatc cacgagccca
     8941 agggctacca tgccaacttc tgtctgggac cctgccccta tatttggagc ctggacacac
     9001 agtacagcaa ggtccttgcc ctctacaacc aacacaaccc gggcgcttcg gcgtcaccgt
     9061 gctgcgtgcc gcaggctttg gagccactgc ccatcgtcta ctacgtgggt cgcaagccca
     9121 aggtggagca gttgtccaac atgattgtgc gctcctgcaa gtgcagctga gatccttgac
     9181 ttgcggcccc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     9241 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     9301 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     9361 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     9421 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     9481 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     9541 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     9601 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     9661 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     9721 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aaatgagcgc tggcgggtcc
     9781 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     9841 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     9901 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     9961 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
    10021 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
    10081 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
    10141 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
    10201 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
    10261 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
    10321 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
    10381 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
    10441 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta gggccgcaac
    10501 tcccacctgc aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcacgagg
    10561 ttaacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
    10621 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
    10681 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
    10741 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
    10801 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
    10861 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
    10921 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
    10981 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
    11041 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgag
    11101 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
    11161 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
    11221 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
    11281 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
    11341 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
    11401 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
    11461 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
    11521 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
    11581 gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa
    11641 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
    11701 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
    11761 cgacctccct agcaaactgg ggcacaagct taattaatgc aggcatgcaa gcttgatatc
    11821 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
    11881 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
    11941 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
    12001 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
    12061 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
    12121 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
    12181 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
    12241 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
    12301 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
    12361 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
    12421 cat
//
