LOCUS       AL179-pHR-Gal4-s       12600 bp DNA     circular SYN 09-JAN-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 23, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12600
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    96..7517
                     /label=match to pHR-CMVLacZ
                     /note="color: #a6acb3"
     primer_bind     complement(320..337)
                     /label=SP6
                     /note="color: #00ff00; direction: LEFT"
     CDS             1231..2091
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1429..2088
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      2186..2868
                     /label=ColE1 origin
                     /note="color: #808080"
     promoter        3084..3410
                     /label=SV40 - Invitrogen promoter
                     /label=SV40 - Invitrogen
                     /note="color: #0000ff"
     promoter        3229..3425
                     /label=SV40 early promoter
                     /note="color: #6495ed"
     polyA_site      5105..5123
                     /label=SV40 pA
                     /note="color: #ccff66"
     polyA_signal    complement(5138..5329)
                     /label=SV40 late polyA
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_site      5199..5330
                     /label=SV40 early poly A
                     /note="color: #80ff00"
     polyA_site      5199..5329
                     /label=SV40 early poly A
                     /label=SV40 early poly A (2)
                     /note="color: #66ff66"
     repeat_region   7518..7697
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     7673..7696
                     /label=cPPT_5'seq
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7721..7737
                     /label=UASBD
                     /note="color: #a6acb3"
     misc_feature    7722..7807
                     /label=Gal4
                     /note="color: #99ccff"
     misc_feature    7848..7853
                     /label=SpeI
                     /note="color: #a6acb3"
     promoter        7854..7971
                     /label=mCMV
                     /note="color: #ffffff"
     primer_bind     7885..7904
                     /label=GMA_CMV.FOR
                     /note="color: black; sequence: agagctcgtttagtgaaccg"
     misc_feature    7972..7991
                     /label=BamHI front
                     /note="color: #a6acb3"
     primer_bind     7977..8003
                     /label=Fragment.FOR
                     /note="color: black; sequence: gacattcgttggatcgccGccatgggc;
                     added: 2019-07-04"
     primer_bind     7977..8000
                     /label=336. AL179.FOR
                     /note="color: black; sequence: gacattcgttggatcgccGccatg; 
                     added: 2019-07-04"
     misc_feature    7986..8669
                     /label=gb12 sTNFR1
                     /note="color: #a6acb3"
     misc_feature    7986..7991
                     /label=BamHI-pHR homology
                     /note="color: #a6acb3"
     misc_feature    7986..7991
                     /label=NBT-1324_synTCR_gBlock
                     /note="color: #ffcc99"
     misc_feature    7988..7991
                     /label=BamHI  back
                     /note="color: #a6acb3"
     misc_feature    7992..7997
                     /label=Kozak Sequence
                     /note="color: #ffff00"
     CDS             7998..8648
                     /codon_start=1
                     /label=sTNFR1-IgG
                     /note="color: #993366"
                     /translation="MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCP
                     QGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSK
                     CRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQN
                     TVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTLVPRGS"
     exon            7998..8630
                     /label=hTNFRIs
                     /note="/vntifkey=""61"" - /label=hTNFRIs - 717..1355"
                     /note="color: #00ff40; direction: RIGHT"
     mat_peptide     8631..8648
                     /label=hexapeptide\thrombine\sensible
                     /note="/vntifkey=""84"" - 
                     /label=hexapeptide\thrombine\sensible - 1356..1373"
                     /note="color: #84a4c0; direction: RIGHT"
     CDS             8649..9344
                     /codon_start=1
                     /label=Human IgG1 Hinge_CH2_CH3
                     /note="http://www.uniprot.org/uniprot/P01857"
                     /note="color: #993300"
                     /translation="EPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVT
                     CVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEY
                     KCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIA
                     VEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYT
                     QKSLSLSPGK"
     primer_bind     complement(9336..9362)
                     /label=337. AL179.REV
                     /note="color: black; sequence: cgcaagtcaaggatcTCACTTGCCCGG;
                     added: 2019-07-04"
     misc_feature    9348..9367
                     /label=BamHI  back
                     /note="color: #a6acb3"
     misc_feature    9366..9939
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
                     /note="color: #a6acb3"
     CDS             9958..10665
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9990..10009)
                     /label=GMA.mCh.Rev
                     /note="color: black; sequence: gcatgaactccttgatgatg"
     misc_feature    10681..10687
                     /label=AarI
                     /note="color: #ff0080"
     misc_feature    10692..10695
                     /label=Part D
                     /note="color: #00ffff"
     primer_bind     10718..10755
                     /label=pHRUASIRESmC_SbfIhomo_pGK_fwd
                     /note="color: black; sequence: 
                     tagagtcgacctgcaCGAGGTTAACGAATTCTACCGGG"
     misc_feature    10753..11252
                     /label=promPGK
                     /note="color: #996633; direction: RIGHT"
     CDS             11273..11971
                     /codon_start=1
                     /gene="TagBFP"
                     /label=TagBFP
                     /note="color: #3366ff"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(11948..11989)
                     /label=pHR_SbfIhomo_tagBFP_rev
                     /note="color: black; sequence: 
                     gcttgcatgccTgcattaattaagcttgtgccccagtttgct"
     enhancer        12009..12600
                     /label=WPRE
                     /note="color: #a6acb3"
     misc_feature    complement(12032..12055)
                     /label=cw11
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     12063..12083
                     /label=QB0112 WPRE-R binding site
                     /note="color: #00ffff; direction: RIGHT"
ORIGIN
        1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
       61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
      301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
      361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
      421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
      481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
      901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
     6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7681 gcagagatcc agtttggatc gataagcttg atatcgaatt cggagcactg tcctccgaac
     7741 gtcggagcac tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct
     7801 ccgaacggag catgtcctcc gaacgtcgga gcactgtcct ccgaacgact agttaggcgt
     7861 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     7921 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctctcgaca
     7981 ttcgttggat cgccgccatg ggcctctcca ccgtgcctga cctgctgctg ccgctggtgc
     8041 tcctggagct gttggtggga atatacccct caggggttat tggactggtc cctcacctag
     8101 gggacaggga gaagagagat agtgtgtgtc cccaaggaaa atatatccac cctcaaaata
     8161 attcgatttg ctgtaccaag tgccacaaag gaacctactt gtacaatgac tgtccaggcc
     8221 cggggcagga tacggactgc agggagtgtg agagcggctc cttcaccgct tcagaaaacc
     8281 acctcagaca ctgcctcagc tgctccaaat gccgaaagga aatgggtcag gtggagatct
     8341 cttcttgcac agtggaccgg gacaccgtgt gtggctgcag gaagaaccag taccggcatt
     8401 attggagtga aaaccttttc cagtgcttca attgcagcct ctgcctcaat gggaccgtgc
     8461 acctctcctg ccaggagaaa cagaacaccg tgtgcacctg ccatgcaggt ttctttctaa
     8521 gagaaaacga gtgtgtctcc tgtagtaact gtaagaaaag cctggagtgc acgaagttgt
     8581 gcctacccca gattgagaat gttaagggca ctgaggactc aggcaccaca ctggttccgc
     8641 gtggatccga gcccaagtcc tgtgataaga ctcatacatg tccgccctgc ccggctcccg
     8701 aacttctcgg cggcccctca gttttcctct tcccccccaa accgaaagac acactgatga
     8761 tcagtaggac cccggaggta acctgcgtag ttgtcgatgt cagccatgag gacccggaag
     8821 ttaaatttaa ttggtatgtc gatggtgtgg aagtacataa tgcgaaaact aagcctcggg
     8881 aggagcagta caatagtacc tatcgggtgg tttccgtcct gaccgttctg catcaggact
     8941 ggcttaacgg aaaggaatac aagtgcaagg tcagtaataa ggcactgcct gctccgattg
     9001 aaaaaacaat atcaaaggct aaagggcagc cgcgcgagcc ccaggtctac acgcttcccc
     9061 ctagtaggga ggaaatgaca aaaaatcaag tctcacttac ttgcttggtt aaggggttct
     9121 acccatcaga catcgcagta gaatgggaga gcaacgggca accggaaaac aactacaaaa
     9181 cgacaccccc agtactggat tctgatggta gttttttctt gtactcaaag ctgaccgtgg
     9241 ataaaagtag gtggcaacag ggaaatgttt tctcttgctc agtcatgcat gaggcactgc
     9301 ataaccatta cacacagaag tcattgagct tgagcccggg caagtgagat ccttgacttg
     9361 cggcccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     9421 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     9481 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     9541 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     9601 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     9661 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     9721 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     9781 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     9841 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     9901 cacggggacg tggttttcct ttgaaaaaca cgatgataaa tgagcgctgg cgggtccatg
     9961 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
    10021 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
    10081 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
    10141 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
    10201 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
    10261 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
    10321 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
    10381 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
    10441 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
    10501 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
    10561 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
    10621 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaggg ccgcaactcc
    10681 cacctgcaac atgcgtgact gactgaggcc gcgactctag agtcgacctg cacgaggtta
    10741 acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
    10801 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
    10861 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
    10921 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
    10981 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
    11041 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
    11101 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
    11161 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
    11221 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgagcga
    11281 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
    11341 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
    11401 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
    11461 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
    11521 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
    11581 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
    11641 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
    11701 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
    11761 gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc
    11821 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
    11881 ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
    11941 cctccctagc aaactggggc acaagcttaa ttaatgcagg catgcaagct tgatatcaag
    12001 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    12061 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    12121 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    12181 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    12241 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    12301 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    12361 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    12421 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    12481 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    12541 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
//
