LOCUS       (selected)_pAAV-U6-Nr1d 5293 bp ds-DNA     circular     16-MAY-2022
DEFINITION  .
COMMENT     Primer design for gRNA Vipr1 3종: 1.1_INF_Mlu1-6-U6_cnF
            ctgcggccgcacgcgtCGTGTAgagggcctatttcccatg 1.1_MG_VIPR1-1-U6_cnR :
            TCGGACCCGCGGTGAGTGTGC GGTGTTTCGTCCTTT 1.2_MG_VIPR1-1-Sca_cnF :
            GCACACTCACCGCGGGTCCGA gttttagagctaGAA 1.2_INF_5-Asc1-5-Sca_cnR
            gcagtctagaACCGGccaggggcgcgccgtcgcTAAAAAAgcaccgactcgg
            2.1_INF_Mlu1-5-Asc1-5-U6_cnF
            ctgcggccgcacgcgtgcgacggcgcgcccctgggagggcctatttcccatg
            2.1_MG_VIPR1-2-U6_cnR : CTTGGGGCCAGGTGGTAGTC GGTGTTTCGTCCTTT
            2.2_MG_VIPR1-2-Sca_cnF : GACTACCACCTGGCCCCAAG gttttagagctaGAA
            2.2_INF-4-Nhe1-5-Sca_cnR
            gcagtctagaACCGGgcatgctagccaccaTAAAAAAgcaccgactcgg
            3.1_INF-Mlu1-5-Nhe1-4-U6_cnF
            ctgcggccgcacgcgttggtggctagcatgcgagggcctatttcccatg
            3.1_MG_VIPR1-3-U6_cnR : CCGCCGTCTTCATCAAGGATC GGTGTTTCGTCCTTT
            3.2_MG_VIPR1-3-Sca_cnF : GATCCTTGATGAAGACGGCGG gttttagagctaGAA
            3.2_INF_Age1_cnR gcagtctagaACCGGT
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    151..155
                     /label="MluI"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     promoter        156..404
                     /label="Human U6 Promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    224..991
                     /label="hU6 sequencing"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    402..404
                     /label="5'"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    405..427
                     /label="Rev-erba gRNA #2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    428..430
                     /label="3'"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    428..509
                     /label="chimeric RNA backbone 1 (SapI)"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    510..515
                     /label="XbaI"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    510..991
                     /label="hSyn"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     exon            1019..1723
                     /label="mCherry"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1019..1723
                     /label="hSyn-F (2 mis)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1748..1996
                     /label="KASH domain of Nesprin2"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2005..2018
                     /label="WPRE"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     polyA_signal    2038..2516
                     /label="hGH polyA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    2556..2696
                     /label="R-ITR"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     rep_origin      2788..3094
                     /label="f1 Origin"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3613..4470
                     /label="AmpR"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     rep_origin      4621..5288
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
      181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
      241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
      301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
      361 tcgatttctt ggctttatat atcttGTGGA AAGGACGAAA CACCGTTGCG ATTGATGCGA
      421 ACGATGGgtt ttagagctaG AAAtagcaag ttaaaataag gctagtccgt tatcaacttg
      481 aaaaagtggc accgagtcgg tgcTTTTTTt ctagactgca gagggccctg cgtatgagtg
      541 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
      601 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
      661 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
      721 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
      781 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
      841 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
      901 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
      961 gtcgtgtcgt gcctgagagc gcagtcgaga aggtaccgga tcctctagag TCGACatggt
     1021 gagcaagggc gaggaggata acatggccat catcGaggag ttcatgcgct tcaaggtgca
     1081 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1141 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     1201 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     1261 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1321 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     1381 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     1441 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1501 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     1561 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     1621 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     1681 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagGAATTCt ccggactcag
     1741 atctcgagag gaggaggagg agacagacag caggatgccc cacctcgaca gccccggcag
     1801 ctcccagccg agacgctcct tcctctcaag ggtgatcagg gcagcgctac cgttgcagct
     1861 gcttctgctg ctgctgctgc tcctggcctg cctgctacct gcctctgaag atgactacag
     1921 ctgcacccag gccaacaact ttgcccgatc cttctacccc atgctgcggt acaccaacgg
     1981 gccacctccc acctagGATa tcaagcttat cgataccgag cgctgctcga gagatctacg
     2041 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     2101 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     2161 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     2221 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     2281 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     2341 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     2401 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     2461 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     2521 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     2581 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     2641 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     2701 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     2761 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2821 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2881 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2941 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     3001 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3061 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     3121 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3181 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     3241 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3301 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3361 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3421 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3481 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3541 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3601 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3661 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3721 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3781 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3841 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3901 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3961 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4021 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4081 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4141 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4201 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4261 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4321 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4381 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4441 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4501 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4561 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4621 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4681 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4741 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4801 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4861 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4921 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4981 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5041 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5101 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5161 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5221 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5281 tttgctcaca tgt
//