LOCUS       223328_pDONOR_MA       10049 bp    DNA        circular     24-JUL-2024
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3988..5707
                     /locus_tag="CAG"
                     /label="CAG"
                     /ApEinfo_label="CAG"
                     /ApEinfo_fwdcolor="#7980ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      1081..1763
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5718..7997
                     /locus_tag="ORF"
                     /label="ORF"
                     /ApEinfo_label="ORF"
                     /ApEinfo_fwdcolor="#ff7d78"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(9594..10034)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5718..6443
                     /locus_tag="Ciona voltage sensitive phosphatase - CiVSP"
                     /label="Ciona voltage sensitive phosphatase - CiVSP"
                     /ApEinfo_label="Ciona voltage sensitive phosphatase -
                     CiVSP"
                     /ApEinfo_fwdcolor="#0096ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             9519..9587
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="cornflower blue"
                     /ApEinfo_revcolor="cornflower blue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7290..7997
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /note="mammalian codon-optimized"
                     /translation=VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGAL
                     KGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYE
                     RAEGRHSTGGMDELYK
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff2f92"
                     /ApEinfo_revcolor="#ff2f92"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    2103..2125
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="cornflower blue"
                     /ApEinfo_revcolor="cornflower blue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7269..7289
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation=PKKKRKV
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             324..983
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7194..7247
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation=EGRGSLLTCGDVEENPGP
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_label="T2A"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2199..3002)
                     /locus_tag="AAVS1 HDR_template"
                     /label="AAVS1 HDR_template"
                     /ApEinfo_label="AAVS1 HDR_template"
                     /ApEinfo_fwdcolor="#4e5fb9"
                     /ApEinfo_revcolor="#4e5fb9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6444..7193
                     /locus_tag="superecliptic pHluorin"
                     /label="superecliptic pHluorin"
                     /ApEinfo_label="superecliptic pHluorin"
                     /ApEinfo_fwdcolor="#00fa92"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3058..3111
                     /locus_tag="T2A Peptide cleavage"
                     /label="T2A Peptide cleavage"
                     /ApEinfo_label="T2A Peptide cleavage"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6882..6884
                     /locus_tag="A389"
                     /label="A389"
                     /ApEinfo_label="A389"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(8569..9388)
                     /locus_tag="AAVS1 HDR teamplate wo T2 binding site"
                     /label="AAVS1 HDR teamplate wo T2 binding site"
                     /ApEinfo_label="AAVS1 HDR teamplate wo T2 binding site"
                     /ApEinfo_fwdcolor="#4e5fb9"
                     /ApEinfo_revcolor="#4e5fb9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6885..6887
                     /locus_tag="A390"
                     /label="A390"
                     /ApEinfo_label="A390"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    8079..8539
                     /locus_tag="Rabbit b-globin pA"
                     /label="Rabbit b-globin pA"
                     /ApEinfo_label="Rabbit b-globin pA"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffa54a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7041..7043
                     /locus_tag="V442"
                     /label="V442"
                     /ApEinfo_label="V442"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5660..5708
                     /locus_tag="rabbit-beta-globin-exon-3"
                     /label="rabbit-beta-globin-exon-3"
                     /ApEinfo_label="rabbit-beta-globin-exon-3"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6075..6134
                     /locus_tag="TM1"
                     /label="TM1"
                     /ApEinfo_label="TM1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          5619..5659
                     /locus_tag="rabbit-beta-globin-intron-2-splice-accepto r"
                     /label="rabbit-beta-globin-intron-2-splice-accepto r"
                     /ApEinfo_label="rabbit-beta-globin-intron-2-splice-accepto
                     r"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6162..6227
                     /locus_tag="TM2"
                     /label="TM2"
                     /ApEinfo_label="TM2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3988..4364
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6267..6329
                     /locus_tag="TM3"
                     /label="TM3"
                     /ApEinfo_label="TM3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4367..4642
                     /locus_tag="chicken-beta-actin-promoter"
                     /label="chicken-beta-actin-promoter"
                     /ApEinfo_label="chicken-beta-actin-promoter"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6360..6392
                     /locus_tag="TM4"
                     /label="TM4"
                     /ApEinfo_label="TM4"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6636..6638
                     /locus_tag="S65T which render it super ecliptic pHluorin"
                     /label="S65T which render it super ecliptic pHluorin"
                     /ApEinfo_label="S65T which render it super ecliptic
                     pHluorin"
                     /ApEinfo_fwdcolor="#0432ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          4658..5614
                     /locus_tag="chicken-beta-actin-intron"
                     /label="chicken-beta-actin-intron"
                     /ApEinfo_label="chicken-beta-actin-intron"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5928..5987
                     /locus_tag="KIR2.1 channel Golgi-to-plasma membrane
                     trafficking signal"
                     /label="KIR2.1 channel Golgi-to-plasma membrane
                     trafficking signal"
                     /ApEinfo_label="KIR2.1 channel Golgi-to-plasma membrane
                     trafficking signal"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3121..3720
                     /locus_tag="PURO"
                     /label="PURO"
                     /ApEinfo_label="PURO"
                     /ApEinfo_fwdcolor="#7980ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    7158..7180
                     /locus_tag="Kir 2.1 channel ER export signal sequence"
                     /label="Kir 2.1 channel ER export signal sequence"
                     /ApEinfo_label="Kir 2.1 channel ER export signal sequence"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3755..3982
                     /locus_tag="bGH_Pa_terminator"
                     /label="bGH_Pa_terminator"
                     /ApEinfo_label="bGH_Pa_terminator"
                     /ApEinfo_fwdcolor="#fefc78"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3015..3033
                     /locus_tag="splice_acceptor"
                     /label="splice_acceptor"
                     /ApEinfo_label="splice_acceptor"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     polyA_signal    8141..8196
                     /locus_tag="rabbit beta globin polyA signal Gil and
                     Proudfoot 1987"
                     /label="rabbit beta globin polyA signal Gil and Proudfoot
                     1987"
                     /ApEinfo_label="rabbit beta globin polyA signal Gil and
                     Proudfoot 1987"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5709..5717
                     /locus_tag="kozak"
                     /label="kozak"
                     /ApEinfo_label="kozak"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3034..3057
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#75d5ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3112..3120
                     /locus_tag="linker(1)"
                     /label="linker(1)"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#75d5ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcaaac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctgtg ctttctctga ccagcattct
     2221 ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac ctctacggct
     2281 ggcccagatc cttccctgcc gcctccttca ggttccgtct tcctccactc cctcttcccc
     2341 ttgctctctg ctgtgttgct gcccaaggat gctctttccg gagcacttcc ttctcggcgc
     2401 tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc tgggtcctct ccgggcatct
     2461 ctcctccctc acccaacccc atgccgtctt cactcgctgg gttccctttt ccttctcctt
     2521 ctggggcctg tgccatctct cgtttcttag gatggccttc tccgacggat gtctcccttg
     2581 cgtcccgcct ccccttcttg taggcctgca tcatcaccgt ttttctggac aaccccaaag
     2641 taccccgtct ccctggcttt agccacctct ccatcctctt gctttctttg cctggacacc
     2701 ccgttctcct gtggattcgg gtcacctctc actcctttca tttgggcagc tcccctaccc
     2761 cccttacctc tctagtctgt gctagctctt ccagccccct gtcatggcat cttccagggg
     2821 tccgagagct cagctagtct tcttcctcca acccgggccc ctatgtccac ttcaggacag
     2881 catgtttgct gcctccaggg atcctgtgtc cccgagctgg gaccacctta tattcccagg
     2941 gccggttaat gtggctctgg ttctgggtac ttttatctgt cccctccacc ccacagtggg
     3001 gcaagcttct gacctcttct cttcctccca cagggcctcg agagatctgg cagcggagag
     3061 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc taggctcgag
     3121 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     3181 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     3241 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     3301 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     3361 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     3421 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     3481 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     3541 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     3601 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     3661 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     3721 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3781 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3841 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3901 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3961 ggggatgcgg tgggctctat gggtcgacat tgattattga ctagttatta atagtaatca
     4021 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     4081 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4141 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     4201 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     4261 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     4321 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     4381 acgttctgct tcactctccc catctccccc cctccccacc cccaattttg tatttattta
     4441 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggcgcg cgccaggcgg
     4501 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     4561 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     4621 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     4681 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     4741 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     4801 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     4861 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     4921 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     4981 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     5041 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     5101 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     5161 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     5221 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     5281 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     5341 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     5401 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     5461 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     5521 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     5581 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     5641 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     5701 tggcaaaggc cgccaccatg gaaggttttg acgggtcaga ctttagccct cctgccgatc
     5761 tcgtgggtgt ggacggtgct gtgatgcgga acgtggtgga cgtgacaatc aacggcgacg
     5821 tgactgcacc acctaaggca gctcctagaa aatctgagag tgtgaagaaa gtccactgga
     5881 acgacgtgga tcagggacca agcgagaagc ccgaaacaag acaggagaag agcaggatca
     5941 ccagcgaggg cgagtacatc cccctggacc agatcgacat caacgtggac gacgggagga
     6001 tggaaattcc taccacagga gtgggtagag tccagtttag ggtgagagct gtcatcgacc
     6061 atctgggaat gcgggtgttc ggcgtctttc tcatcttcct ggacatcatt ctgatgatca
     6121 ttgatctgag tctccctggg aagtcagaga gctcccagag cttctacgac ggcatggcac
     6181 tggccctcag ctgctacttc atgctggatc tcggcctgag gatcttcgca tacggaccca
     6241 agaacttctt taccaatcct tgggaggtgg ccgacggact gatcattgtg gtcacttttg
     6301 tggtcaccat tttctacaca gtgctggacg agtacgtgca ggagacagga gcagacggac
     6361 tcggacagct ggtggtcctc gctcgactgc tccgagtggt ccgactggca aggatctttt
     6421 actcccacca gcaggggaat tcaatgtcta aaggggagga actgttcact ggtgtggtcc
     6481 ccattctggt ggagctcgac ggcgacgtga acggccataa gttttcagtg agcggggagg
     6541 gtgaaggcga cgctacttac ggaaagctca ccctgaaatt catctgcact accgggaagc
     6601 tgccagtgcc ttggcctacc ctggtcacaa ctctcacata cggcgtgcag tgtttcagtc
     6661 gctatcctga ccatatgaag cgacacgatt tctttaaatc tgctatgcca gagggctacg
     6721 tgcaggaacg gaccatcttc tttaaggacg acggcaatta taaaactcgc gcagaggtga
     6781 agtttgaagg cgacaccctg gtcaaccgaa tcgagctcaa gggaattgac ttcaaagaag
     6841 acggaaatat cctggggcac aagctcgagt acaactataa cgccgctcag gtgtacatca
     6901 tggccgataa gcagaaaaac ggcattaagg ctaacttcaa gatccgccac aacattgaag
     6961 acggcggagt gcagctggcc gatcattacc agcagaacac accaatcgga gacgggcctg
     7021 tcctgctccc agataatcac gttctgttca ccacatccac tctgtctaag gaccccaacg
     7081 agaaaagaga tcatatggtg ctgctggagt ttgtcactgc cgacgggatt acccacggaa
     7141 tggacgagct gtataagttc tgctacgaga acgaggtggc atgcggaagc ggagaaggac
     7201 ggggtagtct gctgacatgc ggcgacgtag aagagaatcc tggtcccgcc ccggggtcga
     7261 ccatggtgcc caagaagaag aggaaagtcg tgagcaaggg cgaggaggac aacatggcca
     7321 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     7381 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     7441 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     7501 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     7561 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     7621 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     7681 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     7741 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     7801 tgaaggacgg cggccactac gacgccgagg tcaagaccac ctacaaggcc aagaagcccg
     7861 tgcagctgcc cggcgcctac aacgtcaaca tcaagctgga catcacctcc cacaacgagg
     7921 actacaccat cgtggaacag tacgagcgcg ccgagggccg ccactccacc ggcggcatgg
     7981 acgagctgta caagtaacac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     8041 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     8101 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     8161 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     8221 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     8281 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     8341 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     8401 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     8461 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     8521 tccctcgacc tgcagcccaa gcttggatcc ctcgacagta ctaagctttg acagaaaagc
     8581 cccatcctta ggcctcctcc ttcctagtct cctgatattg ggtctaaccc ccacctcctg
     8641 ttaggcagat tccttatctg gtgacacacc cccatttcct ggagccatct ctctccttgc
     8701 cagaacctct aaggtttgct tacgatggag ccagagagga tcctgggagg gagagcttgg
     8761 cagggggtgg gagggaaggg ggggatgcgt gacctgcccg gttctcagtg gccaccctgc
     8821 gctaccctct cccagaacct gagctgctct gacgcggctg tctggtgcgt ttcactgatc
     8881 ctggtgctgc agcttcctta cacttcccaa gaggagaagc agtttggaaa aacaaaatca
     8941 gaataagttg gtcctgagtt ctaactttgg ctcttcacct ttctagtccc caatttatat
     9001 tgttcctccg tgcgtcagtt ttacctgtga gataaggcca gtagccagcc ccgtcctggc
     9061 agggctgtgg tgaggagggg ggtgtccgtg tggaaaactc cctttgtgag aatggtgcgt
     9121 cctaggtgtt caccaggtcg tggccgcctc tactcccttt ctctttctcc atccttcttt
     9181 ccttaaagag tccccagtgc tatctgggac atattcctcc gcccagagca gggtcccgct
     9241 tccctaaggc cctgctctgg gcttctgggt ttgagtcctt ggcaagccca ggagaggcgc
     9301 tcaggcttcc ctgtccccct tcctcgtcca ccatctcatg cccctggctc tcctgcccct
     9361 tccctacagg ggttcctggc tctgctctag cgatcgccaa ttcgccctat agtgagtcgt
     9421 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     9481 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     9541 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     9601 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     9661 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     9721 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     9781 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     9841 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     9901 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9961 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
    10021 acaaaatatt aacgcttaca atttaggtg   
//
