LOCUS       Exported                6859 bp ds-DNA     circular SYN 11-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Marie-Eve Paquet
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 28, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     promoter        162..842
                     /label=gfaABC1D
                     /note="GFAP promoter"
     promoter        906..924
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      949..958
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             967..1029
                     /codon_start=1
                     /label=IgK
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             1036..2793
                     /codon_start=1
                     /label=R-eLACCO2
                     /translation="MFSPMAVAQARRYKWRIQTAWDAGTVGYSLFQKFTERVKELTDGQ
                     LEVQPLPAGAVVGTFDMFDAVKTGVLDGMNPFTLYWAERMPVTAFLSSYALGLDRPDQW
                     ETWFYSLGGLDNARRAFAEQGLFYVGPVQHDLNTIHSKKPIRRFEDFKGVKLRVPGGMI
                     AEVFAAAGASTVLLPGGEVYPALERGAVTAVSERMYPEDGALKSEIKKGLRLKDGGHYA
                     AVVKTTYKAKKPVQLPGAYVVDIQLDIVSHNEDYTIVEQCERAEGRHSTGGVVELDKGG
                     TGGSLVSKGEEDDMAIVKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTK
                     GGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMYFEDGGIIHVDQ
                     DSSLQDGVFIYKVKLRGTNFPPDGPVMQKKTMGWERGRSAADFVGPAVNYNLGFHQEAK
                     YIIMGPPETPAIHQPVDLMDFTINLNRWRSLPKPLQERFIAAVHEYSWIHYAGIQKANL
                     EAWPKYRQAGVEVIRLGNEDVRKFRRLAIPIWFKWAKMDKYSREAFASQLEYMRGIGHV
                     TDEELKGLSL"
     CDS             2854..2970
                     /codon_start=1
                     /label=GPI_COBL9 Anchor
                     /translation="SDNDYGKDGLAGASGGRRNGAITVLSFITFYVAAFMVLL"
     misc_feature    2976..3584
                     /label=WPRE
     polyA_signal    3604..4082
                     /label=hGH polyA
     misc_feature    4122..4262
                     /label=R-ITR
     rep_origin      4354..4660
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    5179..6036
                     /label=AmpR
     rep_origin      6187..6854
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggatc taacatatcc tggtgtggag
      181 taggggacgc tgctctgaca gaggctcggg ggcctgagct ggctctgtga gctggggagg
      241 aggcagacag ccaggccttg tctgcaagca gacctggcag cattgggctg gccgcccccc
      301 agggcctcct cttcatgccc agtgaatgac tcaccttggc acagacacaa tgttcggggt
      361 gggcacagtg cctgcttccc gccgcacccc agcccccctc aaatgccttc cgagaagccc
      421 attgagcagg gggcttgcat tgcaccccag cctgacagcc tggcatcttg ggataaaagc
      481 agcacagccc cctaggggct gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc
      541 tctattcagc ctgtgcccag gaaaggggat caggggatgc ccaggcatgg acagtgggtg
      601 gcaggggggg agaggagggc tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg
      661 ggagagctct ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg
      721 ggtgttgcca ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc
      781 atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc
      841 gcggggatcc accggtgagc tctctggcta actagagaac ccactgctta ctggcttatc
      901 gaaattaata cgactcacta tagggagacc caagctggct agcgtgccgc caccatggcc
      961 tcgagaatgg agaccgacac cctgctgctg tgggtgctgc tgctgtgggt gcccggctcc
     1021 accggcgaca gatctatgtt ctctcccatg gcagtggccc aggctcgacg ctataagtgg
     1081 aggattcaaa ccgcgtggga tgctggaaca gtaggctaca gtctgttcca aaagtttacg
     1141 gagcgggtaa aggaactgac ggatggacag cttgaggtcc agcctcttcc agcgggtgcg
     1201 gtcgtaggca cgtttgatat gttcgacgcg gtcaagactg gagttttgga cggaatgaat
     1261 cctttcacgc tgtattgggc agagaggatg ccggttacag ctttcctctc cagctacgcc
     1321 ctcgggttgg accgacctga ccagtgggaa acatggtttt actccctggg tggcctcgac
     1381 aacgcccgac gagcctttgc cgagcaaggc ctcttctacg tgggaccagt tcagcacgac
     1441 ctcaatacaa ttcattcaaa gaagccgatc cggagatttg aggacttcaa aggtgtcaag
     1501 ctccgagtgc caggcggcat gattgcggaa gtctttgcgg ccgcaggagc ttccaccgtc
     1561 ctcttgcctg ggggggaggt ctaccccgct ctggaaagag gggcagtgac tgcggtttcc
     1621 gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaaggg gctgaggctg
     1681 aaggacggcg gccactacgc cgccgtggtc aagaccacct acaaggccaa gaagcccgtg
     1741 cagctgcccg gcgcctacgt cgtcgacatc cagttggaca tcgtgtccca caacgaggac
     1801 tacactatcg tggaacagtg cgaacgcgcc gagggccgcc actccaccgg cggcgtggtc
     1861 gagctggaca agggaggtac aggcgggagt ctggtgagca agggcgagga ggatgacatg
     1921 gccatcgtca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     1981 gagttcgaga tcgagggcga gggcgagggc cgcccctacg aggcctttca gaccgctaag
     2041 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctatc ccctcagttc
     2101 atgtacggct ccaaggccta cattaagcac ccagccgaca tccccgacta cttcaagctg
     2161 tccttccccg agggcttcag gtgggagcgc gtgatgtact tcgaggacgg cggcattatt
     2221 cacgttgacc aggactcctc cctgcaggac ggcgtattca tctacaaggt gaagctgcgc
     2281 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggagcgg
     2341 gggcgtagtg cggccgattt tgttgggcct gcagtaaact ataacttggg gttccatcag
     2401 gaggcaaagt acatcataat ggggcctcca gagacgcctg ccattcatca acctgtagac
     2461 cttatggact tcaccataaa ccttaatcgg tggcgcagcc ttccaaagcc tctgcaagaa
     2521 agattcatcg cggcggttca cgagtactct tggattcact acgcaggcat tcagaaggct
     2581 aatttggagg cgtggccgaa atatcggcag gcaggtgttg aggtcatccg gctgggcaac
     2641 gaggatgtta gaaaattcag gcgactcgct atccccattt ggtttaagtg ggctaagatg
     2701 gataaatact cacgagaggc tttcgcgtcc caactggagt atatgagagg tataggtcac
     2761 gttacggacg aggaattgaa gggcttgagc ctgggctcca cctccggctc cggcaagccc
     2821 ggctccggcg agggctccac caagggcgaa ttctcagata acgactacgg taaagatggg
     2881 ttggctgggg cttccggagg cagacgaaat ggcgctataa ctgtgctcag tttcataacg
     2941 ttctacgtgg cagcatttat ggtattgttg tagaagctta tcgataatca acctctggat
     3001 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3061 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3121 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3181 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3241 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3301 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3361 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3421 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3481 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3541 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga
     3601 tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     3661 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     3721 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     3781 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     3841 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     3901 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     3961 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     4021 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     4081 ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga
     4141 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     4201 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     4261 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     4321 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4381 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4441 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4501 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     4561 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4621 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     4681 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4741 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     4801 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     4861 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4921 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     4981 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5041 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5101 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5161 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5221 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5401 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5881 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6841 tggccttttg ctcacatgt
//
