LOCUS       Exported                6681 bp ds-DNA     circular SYN 11-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Marie-Eve Paquet
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 28, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     misc_feature    156..640
                     /label=hSyn
     promoter        728..746
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      771..780
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             789..851
                     /codon_start=1
                     /label=IgK
                     /translation="METDTLLLWVLLLWVPGSTGD"
     misc_feature    858..2615
                     /label=R-deLACCO1
     CDS             2676..2792
                     /codon_start=1
                     /label=GPI_COBL9 Anchor
                     /translation="SDNDYGKDGLAGASGGRRNGAITVLSFITFYVAAFMVLL"
     misc_feature    2802..3406
                     /label=WPRE
     polyA_signal    3426..3904
                     /label=hGH polyA
     misc_feature    3944..4084
                     /label=R-ITR
     rep_origin      4176..4482
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    5001..5858
                     /label=AmpR
     rep_origin      6009..6676
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cggacgaata
      661 acgcgtggat ccaccggtga gctctctggc taactagaga acccactgct tactggctta
      721 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgtgcc gccaccatgg
      781 cctcgagaat ggagaccgac accctgctgc tgtgggtgct gctgctgtgg gtgcccggct
      841 ccaccggcga cagatctatg ttctctccca tggcagtggc ccaggctcga cgctataagt
      901 ggaggattca aaccgcgtgg gatgctggaa cagtaggcta cagtctgttc caaaagttta
      961 cggagcgggt aaaggagctg acggatggtc agcttgaggt ccagcctctt ccagcgggtg
     1021 cggtcgtagg cacgtttgat atgttcgacg cggtcaagac tggagttttg gacggaatga
     1081 atcctttcac gaactattgg gcagagagga tgccggttac agctttcctc tccagctacg
     1141 ccctcgggtt ggaccgacct gaccagtggg aaacatggtt ttactccctg ggtggcctcg
     1201 acaacgcccg acgagccttt gccgagcaag gcctcttcta cgtgggacca gttcagcacg
     1261 acctcaatac aattcattca aagaagccga tccggagatt tgaggacttc aaaggtgtca
     1321 agctccgagt gccaggcggc atgattgcgg aagtctttgc ggccgcagga gcttccaccg
     1381 tcctcttgcc tgggggggag gtcaaccccg ctctggaaag aggggcagtg actgcggttt
     1441 ccgagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag gggctgaggc
     1501 tgaaggacgg cggccactac gccgccgtgg tcaagaccac ctacaaggcc aagaagcccg
     1561 tgcagctgcc cggcgcctac gtcgtcgaca tccggttgga catcgtgtcc cacaacgagg
     1621 actacactat cgtggaacag tgcgaacgcg ccgagggccg ccactccacc ggcggcgtgg
     1681 tcgagctgga caagggaggt acaggcggga gtctgttgag caagggcgag gaggttgaca
     1741 tggccatcgt caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1801 acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta
     1861 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatccta tcccctcagt
     1921 tcatgtacgg ctccaaggcc tacattaagc acccagccga catccccgac tacttcaagc
     1981 tgtccttccc cgagggcttc aggtgggagc gcgtgatgta cttcgaggac ggcggcatta
     2041 ttcacgttga ccaggactcc tccctgcagg acggcgtatt catctacaag gtgaagctgc
     2101 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagc
     2161 gggggcgtag tgcggccaac tttgtcgggc ctgcagtaaa ctataacttg gggttccatc
     2221 aggaggcaaa gtacatcata atggggcctc cagagacgcc tgccattcat caacctgtag
     2281 accttatgga cttcaccata aaccttaatc ggtggcgaag ccttccaaag cctctgcaag
     2341 aaagattcat cgcggcggtt cacgagtact cttggattca ctacgcaggc attcggaagg
     2401 ctaatttgga ggcgtggccg aaatatcggc aggcaggtgt agaggtcatc cggctgggca
     2461 acgaggatgt tagaaagttc aggcgacttg ctatccccat ttggtttaag tgggctaaga
     2521 tggataaata ctcacgagag gctttcgcgt cccaactgga gtatatgaga ggtataggtc
     2581 acgttacgga cgaggaattg aagggcttga gcctgggctc cacctccggc tccggcaagc
     2641 ccggctccgg cgagggctcc accaagggcg aattctcaga taacgactac ggtaaagatg
     2701 ggttggctgg ggcttccgga ggcagacgaa atggcgctat aactgtgctc agtttcataa
     2761 cgttctacgt ggcagcattt atggtattgt tgtagaagct tatcgataat caacctctgg
     2821 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2881 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2941 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3001 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3061 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3121 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3181 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     3241 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3301 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3361 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
     3421 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     3481 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     3541 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     3601 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     3661 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     3721 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     3781 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     3841 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     3901 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     3961 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     4021 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     4081 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4141 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4201 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4261 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4321 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4381 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4441 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4501 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4561 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4621 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4681 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4741 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4801 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4861 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4921 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4981 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5041 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5101 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5161 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5221 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5281 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5341 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5401 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5461 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5521 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5581 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5641 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5701 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5761 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5821 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5881 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5941 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6661 gctggccttt tgctcacatg t
//
