LOCUS       pYPQAT09       16028 bp DNA     circular SYN 28-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16028)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16028
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    2301..2306
                     /label=Esp3I recognition site(1)
                     /note="Esp3I recognition site"
     misc_feature    2764
                     /label=G to C
                     /note="To kill BsaI site"
     misc_feature    complement(5150..5155)
                     /label=Esp3I recognition site
                     /note="Esp3I recognition site"
     misc_feature    complement(6234..6259)
                     /label=RightBorder
                     /note="RightBorder"
     misc_feature    complement(6338..6500)
                     /label=LacZpromoter(1)
                     /note="LacZpromoter"
     primer_bind     6480..6497
                     /label=M13-fwd
                     /note="M13-fwd"
     promoter        6519..7287
                     /label=2X35S promoter
                     /note="2X35S promoter"
     repeat_region   7323..7335
                     /label=attR1
                     /note="attR1"
     protein_bind    7328..7352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     7336..7352
                     /label=attL1
                     /note="/ApEinfo_fwdcolor=#00ff40 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=attL1 
                     /invitrogen=2670000 /vntifkey=86"
     sig_peptide     7374..7439
                     /label=3xFLAG
     misc_feature    7446..7466
                     /label=SV40 NLS
     CDS             7491..11591
                     /codon_start=1
                     /label=zCas9
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    7668..7670
                     /label=A61R
     misc_feature    10818..10820
                     /label=L1111R
     misc_feature    10890..10892
                     /label=D1135L
     misc_feature    10893..10895
                     /label=S1136W
     misc_feature    11139..11141
                     /label=G1218K
     misc_feature    11142..11144
                     /label=E1219Q
     misc_feature    11436..11438
                     /label=N1317R
     misc_feature    11451..11453
                     /label=A1322R
     misc_feature    11484..11486
                     /label=R1333P
     misc_feature    11490..11492
                     /label=R1335Q
     misc_feature    11496..11498
                     /label=T1337R
     misc_feature    11592..11639
                     /label=nucleoplasmin NLS
     terminator      11647..12267
                     /label=rbcS-E9t
                     /note="/dnas_title=rbcS-E9t /vntifkey=43"
     protein_bind    12314..12334
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     misc_recomb     12314..12320
                     /label=attR5
                     /note="/ApEinfo_fwdcolor=#00ff00 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 /ApEinfo_revcolor=pink /dnas_title=attR5 
                     /invitrogen=2703000 /vntifkey=86"
     misc_feature    12321..12337
                     /label=attL5
                     /note="/dnas_title=attL5"
                     /note="/vntifkey=21"
     misc_feature    12351..12363
                     /label=For Left homology arm
                     /label=nonstandard type: insertion_seq
                     /note="/dnas_title=For Left homology arm"
                     /note="/vntifkey=14"
     promoter        12371..12696
                     /label=AtU3 promoter
                     /note="AtU3 promoter"
     modified_base   12446
                     /label=G to c
                     /note="G to c"
     misc_feature    12632..12641
                     /label=USE
                     /note="USE"
     TATA_signal     12666..12673
                     /note="TATA box"
     misc_feature    12697
                     /label=first necletide transcript
                     /note="first necletide transcript"
     misc_feature    12698..12709
                     /label=FUS_A_left
                     /note="FUS_A_left"
     misc_feature    complement(12725..13000)
                     /label=lacZ'
                     /note="lacZ'"
     promoter        complement(13001..13122)
                     /label=lacZ promoter
                     /note="lacZ promoter"
     misc_structure  13151
                     /label=guide RNA cloning site
                     /note="guide RNA cloning site"
     misc_feature    13152..13227
                     /label=guide RNA scaffold
                     /note="guide RNA scaffold"
     terminator      13228..13409
                     /note="AtU3 terminator"
     protein_bind    complement(13430..13454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    13430..13439
                     /label=attL2
                     /note="/dnas_title=attL2"
                     /note="/vntifkey=21"
     repeat_region   13440..13454
                     /label=attR2
                     /note="attR2"
     terminator      complement(13508..13747)
                     /label=nos terminator
                     /note="nos terminator"
     terminator      complement(13748..13765)
                     /label=nos terminator(1)
                     /note="nos terminator"
     misc_feature    complement(13794..13857)
                     /label=LacZpromoter
                     /note="LacZpromoter"
     primer_bind     complement(13850..13869)
                     /label=M13-rev
                     /note="M13-rev"
     misc_binding    complement(13875..13897)
                     /label=LacO
                     /note="LacO"
     misc_feature    complement(14087..14867)
                     /label=2X35S Promoter
                     /note="2X35S Promoter"
     misc_feature    complement(14898..15695)
                     /label=nptII (kanR)
                     /note="nptII"
     misc_feature    complement(15733..15936)
                     /label=CaMV 3'UTR(polyA signal)
                     /note="CaMV 3'UTR"
     misc_feature    complement(16003..16028)
                     /label=LeftBorder
                     /note="LeftBorder"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg
     3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
     5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
     5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
     5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
     5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
     6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
     6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
     6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc
     6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
     6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6481 gtaaaacgac ggccagtgcc aagcttggcg tgcctgcagg tcaacatggt ggagcacgac
     6541 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     6601 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     6661 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     6721 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     6781 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     6841 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
     6901 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
     6961 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
     7021 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
     7081 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     7141 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     7201 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     7261 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgccaag
     7321 ctatcaaaca agtttgtaca aaaaagcagg ctccgaattc gcccttcacc atggattaca
     7381 aggaccacga cggggattac aaggaccacg acattgatta caaggatgat gatgacaaga
     7441 tggctccgaa gaagaagagg aaggttggca tccacggggt gccagctgct gacaagaagt
     7501 actcgatcgg cctcgatatt gggactaact ctgttggctg ggccgtgatc accgacgagt
     7561 acaaggtgcc ctcaaagaag ttcaaggtcc tgggcaacac cgatcggcat tccatcaaga
     7621 agaatctcat tggcgctctc ctgttcgaca gcggcgagac ggctgagagg acgcggctca
     7681 agcgcaccgc ccgcaggcgg tacacgcgca ggaagaatcg catctgctac ctgcaggaga
     7741 ttttctccaa cgagatggcg aaggttgacg attctttctt ccacaggctg gaggagtcat
     7801 tcctcgtgga ggaggataag aagcacgagc ggcatccaat cttcggcaac attgtcgacg
     7861 aggttgccta ccacgagaag taccctacga tctaccatct gcggaagaag ctcgtggact
     7921 ccacagataa ggcggacctc cgcctgatct acctcgctct ggcccacatg attaagttca
     7981 ggggccattt cctgatcgag ggggatctca acccggacaa tagcgatgtt gacaagctgt
     8041 tcatccagct cgtgcagacg tacaaccagc tcttcgagga gaaccccatt aatgcgtcag
     8101 gcgtcgacgc gaaggctatc ctgtccgcta ggctctcgaa gtctcggcgc ctcgagaacc
     8161 tgatcgccca gctgccgggc gagaagaaga acggcctgtt cgggaatctc attgcgctca
     8221 gcctggggct cacgcccaac ttcaagtcga atttcgatct cgctgaggac gccaagctgc
     8281 agctctccaa ggacacatac gacgatgacc tggataacct cctggcccag atcggcgatc
     8341 agtacgcgga cctgttcctc gctgccaaga atctgtcgga cgccatcctc ctgtctgata
     8401 ttctcagggt gaacaccgag attacgaagg ctccgctctc agcctccatg atcaagcgct
     8461 acgacgagca ccatcaggat ctgaccctcc tgaaggcgct ggtcaggcag cagctccccg
     8521 agaagtacaa ggagatcttc ttcgatcagt cgaagaacgg ctacgctggg tacattgacg
     8581 gcggggcctc tcaggaggag ttctacaagt tcatcaagcc gattctggag aagatggacg
     8641 gcacggagga gctgctggtg aagctcaatc gcgaggacct cctgaggaag cagcggacat
     8701 tcgataacgg cagcatccca caccagattc atctcgggga gctgcacgct atcctgagga
     8761 ggcaggagga cttctaccct ttcctcaagg ataaccgcga gaagatcgag aagattctga
     8821 ctttcaggat cccgtactac gtcggcccac tcgctagggg caactcccgc ttcgcttgga
     8881 tgacccgcaa gtcagaggag acgatcacgc cgtggaactt cgaggaggtg gtcgacaagg
     8941 gcgctagcgc tcagtcgttc atcgagagga tgacgaattt cgacaagaac ctgccaaatg
     9001 agaaggtgct ccctaagcac tcgctcctgt acgagtactt cacagtctac aacgagctga
     9061 ctaaggtgaa gtatgtgacc gagggcatga ggaagccggc tttcctgtct ggggagcaga
     9121 agaaggccat cgtggacctc ctgttcaaga ccaaccggaa ggtcacggtt aagcagctca
     9181 aggaggacta cttcaagaag attgagtgct tcgattcggt cgagatctct ggcgttgagg
     9241 accgcttcaa cgcctccctg gggacctacc acgatctcct gaagatcatt aaggataagg
     9301 acttcctgga caacgaggag aatgaggata tcctcgagga cattgtgctg acactcactc
     9361 tgttcgagga ccgggagatg atcgaggagc gcctgaagac ttacgcccat ctcttcgatg
     9421 acaaggtcat gaagcagctc aagaggagga ggtacaccgg ctgggggagg ctgagcagga
     9481 agctcatcaa cggcattcgg gacaagcagt ccgggaagac gatcctcgac ttcctgaaga
     9541 gcgatggctt cgcgaaccgc aatttcatgc agctgattca cgatgacagc ctcacattca
     9601 aggaggatat ccagaaggct caggtgagcg gccaggggga ctcgctgcac gagcatatcg
     9661 cgaacctcgc tggctcgcca gctatcaaga aggggattct gcagaccgtg aaggttgtgg
     9721 acgagctggt gaaggtcatg ggcaggcaca agcctgagaa catcgtcatt gagatggccc
     9781 gggagaatca gaccacgcag aagggccaga agaactcacg cgagaggatg aagaggatcg
     9841 aggagggcat taaggagctg gggtcccaga tcctcaagga gcacccggtg gagaacacgc
     9901 agctgcagaa tgagaagctc tacctgtact acctccagaa tggccgcgat atgtatgtgg
     9961 accaggagct ggatattaac aggctcagcg attacgacgt cgatcatatc gttccacagt
    10021 cattcctgaa ggatgactcc attgacaaca aggtcctcac caggtcggac aagaaccggg
    10081 gcaagtctga taatgttcct tcagaggagg tcgttaagaa gatgaagaac tactggcgcc
    10141 agctcctgaa tgccaagctg atcacgcagc ggaagttcga taacctcaca aaggctgaga
    10201 ggggcgggct ctctgagctg gacaaggcgg gcttcatcaa gaggcagctg gtcgagacac
    10261 ggcagatcac taagcacgtt gcgcagattc tcgactcacg gatgaacact aagtacgatg
    10321 agaatgacaa gctgatccgc gaggtgaagg tcatcaccct gaagtcaaag ctcgtctccg
    10381 acttcaggaa ggatttccag ttctacaagg ttcgggagat caacaattac caccatgccc
    10441 atgacgcgta cctgaacgcg gtggtcggca cagctctgat caagaagtac ccaaagctcg
    10501 agagcgagtt cgtgtacggg gactacaagg tttacgatgt gaggaagatg atcgccaagt
    10561 cggagcagga gattggcaag gctaccgcca agtacttctt ctactctaac attatgaatt
    10621 tcttcaagac agagatcact ctggccaatg gcgagatccg gaagcgcccc ctcatcgaga
    10681 cgaacggcga gacgggggag atcgtgtggg acaagggcag ggatttcgcg accgtcagga
    10741 aggttctctc catgccacaa gtgaatatcg tcaagaagac agaggtccag actggcgggt
    10801 tctctaagga gtcaattagg cctaagcgga acagcgacaa gctcatcgcc cgcaagaagg
    10861 actgggatcc gaagaagtac ggcgggttcc tgtggcccac tgtggcctac tcggtcctgg
    10921 ttgtggcgaa ggttgagaag ggcaagtcca agaagctcaa gagcgtgaag gagctgctgg
    10981 ggatcacgat tatggagcgc tccagcttcg agaagaaccc gatcgatttc ctggaggcga
    11041 agggctacaa ggaggtgaag aaggacctga tcattaagct ccccaagtac tcactcttcg
    11101 agctggagaa cggcaggaag cggatgctgg cttccgctaa gcagctgcag aaggggaacg
    11161 agctggctct gccgtccaag tatgtgaact tcctctacct ggcctcccac tacgagaagc
    11221 tcaagggcag ccccgaggac aacgagcaga agcagctgtt cgtcgagcag cacaagcatt
    11281 acctcgacga gatcattgag cagatttccg agttctccaa gcgcgtgatc ctggccgacg
    11341 cgaatctgga taaggtcctc tccgcgtaca acaagcaccg cgacaagcca atcagggagc
    11401 aggctgagaa tatcattcat ctcttcaccc tgacgaggct cggcgcccct agggctttca
    11461 agtacttcga cacaactatc gatccgaagc agtacaggag cactaaggag gtcctggacg
    11521 cgaccctcat ccaccagtcg attaccggcc tctacgagac gcgcatcgac ctgtctcagc
    11581 tcgggggcga caagcggcca gcggcgacga agaaggcggg gcaggcgaag aagaagaagt
    11641 gagctcagag ctttcgttcg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc
    11701 agtttcattg cgcacacacc agaatcctac tgagtttgag tattatggca ttgggaaaac
    11761 tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc
    11821 tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt
    11881 tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat
    11941 tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat
    12001 gaccgaagtt aatatgagga gtaaaacact tgtagttgta ccattatgct tattcactag
    12061 gcaacaaata tattttcaga cctagaaaag ctgcaaatgt tactgaatac aagtatgtcc
    12121 tcttgtgttt tagacattta tgaactttcc tttatgtaat tttccagaat ccttgtcaga
    12181 ttctaatcat tgctttataa ttatagttat actcatggat ttgtagttga gtatgaaaat
    12241 attttttaat gcattttatg acttgccaat tgttccggaa ctctagataa gcttaccgga
    12301 aagggcgaat tcgcaacttt gtatacaaaa gttgccccat ggcgttccct ctagataacg
    12361 caggatccta tttactttaa atttttctta tggctcagcc tgtgatggat aactgaatca
    12421 aacaaatggc gtctgggttt aagaacatct gttttggcta tgttggacga aacaagtgaa
    12481 cttttaggat caacttccgt ttatatacgg agcttatatc gagcaataag ataagtgggc
    12541 tttttatgta atttaatggg ctatcgtcca tatattcact aatacccatg cccagtaccc
    12601 atgtatgcgt ttcatataag ctcctaattt ctcccacatc gctcaaatct aaacaaatct
    12661 tgttgtatat ataacactga gggagcacca ttggtcagag accggcgccg ctacagggcg
    12721 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    12781 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    12841 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
    12901 cactataggg cgaattgggt accgggcccc ccctcgaggt cctccagctt ttgttccctt
    12961 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    13021 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    13081 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccac
    13141 cggtggtctc tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
    13201 cttgaaaaag tggcaccgag tcggtgcttt tttttccctt tccttttttc ttttttttgc
    13261 cataaactta aatttgtata tcgatcattg tagatattga aaacctagaa caaaccaaca
    13321 tccatgtgaa tgtctttcat gactgattta gagataattc ttgaattttg gaactagaat
    13381 ctataatgag cctaaattaa aacattgtga actagtaagg gcgaattcga cccagctttc
    13441 ttgtacaaag tggttcgata attcttaatt aactagttct agagcggccg ccaccgcggt
    13501 ggagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
    13561 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
    13621 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
    13681 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
    13741 cgcgcgcggt gtcatctatg ttactgaatt cgtaatcatg gtcatagaag cttgcatgcc
    13801 tgcaggtcga ctctagagga tccccgggta ccgagctcga attcgtaatc atgtcatagc
    13861 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    13921 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    13981 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    14041 gcgcggggag aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga
    14101 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
    14161 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
    14221 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
    14281 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
    14341 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
    14401 ttgatgtgat aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
    14461 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
    14521 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
    14581 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
    14641 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
    14701 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
    14761 cgcacaatcc cactatcctt cgcaagacct tcctctatat aaggaagttc atttcatttg
    14821 gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc
    14881 agatctgtcg atcgaccatg gggattgaac aagatggatt gcacgcaggt tctccggccg
    14941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
    15001 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
    15061 ccggtgccct gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg
    15121 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
    15181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
    15241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    15301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    15361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    15421 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc
    15481 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    15541 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    15601 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    15661 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcggatcgat
    15721 cctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
    15781 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
    15841 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
    15901 caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta
    15961 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
    16021 tcctgcca
//
