LOCUS       pYPQAT21       16620 bp DNA     circular SYN 28-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16620)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16620
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    2301..2306
                     /label=Esp3I recognition site(1)
                     /note="Esp3I recognition site"
     misc_feature    2764
                     /label=G to C
                     /note="To kill BsaI site"
     misc_feature    complement(5150..5155)
                     /label=Esp3I recognition site
                     /note="Esp3I recognition site"
     misc_feature    complement(6234..6259)
                     /label=RightBorder
                     /note="RightBorder"
     misc_feature    complement(6338..6500)
                     /label=LacZpromoter(1)
                     /note="LacZpromoter"
     primer_bind     6480..6497
                     /label=M13-fwd
                     /note="M13-fwd"
     promoter        6519..7287
                     /label=2X35S promoter
                     /note="2X35S promoter"
     repeat_region   7323..7335
                     /label=attR1
                     /note="attR1"
     protein_bind    7328..7352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     7336..7352
                     /label=attL1
                     /note="attL1
                     vntifkey=86
                     ApEinfo_revcolor=pink
                     invitrogen=2670000
                     ApEinfo_fwdcolor=#00ff40
                     dnas_title=attL1
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     CDS             7368..7964
                     /codon_start=1
                     /product="Rice codon optimized"
                     /label=hA3A
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIFDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     misc_feature    7755..7757
                     /product="Mutation to narrow base editing window"
                     /label=Y130F
     CDS             7965..8012
                     /codon_start=1
                     /label=XTEN linker
                     /note="XTEN linker"
                     /translation="SGSETPGTSESATPES"
     sig_peptide     8019..8084
                     /label=3xFLAG
     misc_feature    8091..8111
                     /label=SV40 NLS
     CDS             8147..12207
                     /codon_start=1
                     /label=zCas9
                     /translation="LDRPRYWD*LCWLGRDHRRVQGALKEVQGPGQHRSAFHQEESHWR
                     SPVRQRRDG*EDAAQAHRPQAVHAQEESHLLPAGDFLQRDGEG*RFFLPQAGGVIPRGG
                     G*EARAASNLRQHCRRGCLPREVPYDLPSAEEARGLHR*GGPPPDLPRSGPHD*VQGPF
                     PDRGGSQPGQ*RC*QAVHPARADVQPALRGEPH*CVRRRREGYPVR*ALEVSAPREPDR
                     PAAGREEERPVRESHCAQPGAHAQLQVEFRSR*GRQAAALQGHIRR*PG*PPGPDRRSV
                     RGPVPRCQESVGRHPPV*YSQGEHRDYEGSALSLHDQALRRAPSGSDPPEGAGQAAAPR
                     EVQGDLLRSVEERLRWVH*RRGLSGGVLQVHQADSGEDGRHGGAAGEAQSRGPPEEAAD
                     IR*RQHPTPDSSRGAARYPEEAGGLLPFPQG*PREDREDSDFQDPVLRRPTR*GQLPLR
                     LDDPQVRGDDHAVELRGGGRQGR*RSVVHREDDEFRQEPAK*EGAP*ALAPVRVLHSLQ
                     RAD*GEVCDRGHEEAGFPVWGAEEGHRGPPVQDQPEGHG*AAQGGLLQED*VLRFGRDL
                     WR*GPLQRLPGDLPRSPEDH*G*GLPGQRGE*GYPRGHCADTHSVRGPGDDRGAPEDLR
                     PSLR*QGHEAAQEEEVHRLGEAEQEAHQRHSGQAVREDDPRLPEERWLREPQFHAADSR
                     *QPHIQGGYPEGSGERPGGLAARAYREPRWLASYQEGDSADREGCGRAGEGHGQAQA*E
                     HRH*DGPGESDHAEGPEELTREDEEDRGGH*GAGVPDPQGAPGGEHAAAE*EALPVLPP
                     EWPRYVCGPGAGY*QAQRLRRRSYRSTVIPEG*LH*QQGPHQVGQEPGQV**CSFRGGR
                     *EDEELLAPAPECQADHAAEVR*PHKG*EGRAL*AGQGGLHQEAAGRDTADH*ARCADS
                     RLTDEH*VR*E*QADPRGEGHHPEVKARLRLQEGFPVLQGSGDQQLPPCP*RVPERGGR
                     HSSDQEVPKARERVRVRGLQGLRCEEDDRQVGAGDWQGYRQVLLLL*HYEFLQDRDHSG
                     QWRDPEAPPHRDERRDGGDRVGQGQGFRDRQEGSLHATSEYRQEDRGPDWRVL*GVN*A
                     *AEQRQAHRPQEGLGSEEVRRVPVAHCGLLGPGCGEG*EGQVQEAQEREGAAGDHDYGA
                     LQLREEPDRFPGGEGLQGGEEGPDH*APQVLTLRAGERQEADAGFR*AAAEGERAGSAV
                     QVCELPLPGLPLREAQGQPRGQRAEAAVRRAAQALPRRDH*ADFRVLQARDPGRRESG*
                     GPLRVQQAPRQANQGAG*EYHSSLHPDEARRP*GFQVLRHNYRSEAVQEH*GGPGRDPH
                     PPVDYRPLRD"
     misc_feature    8160..8162
                     /label=D10A
     misc_feature    8313..8315
                     /label=A61R
     misc_feature    11463..11465
                     /label=L1111R
     misc_feature    11535..11537
                     /label=D1135L
     misc_feature    11538..11540
                     /label=S1136W
     misc_feature    11784..11786
                     /label=G1218K
     misc_feature    11787..11789
                     /label=E1219Q
     misc_feature    12081..12083
                     /label=N1317R
     misc_feature    12096..12098
                     /label=A1322R
     misc_feature    12129..12131
                     /label=R1333P
     misc_feature    12135..12137
                     /label=R1335Q
     misc_feature    12141..12143
                     /label=T1337R
     misc_feature    12237..12284
                     /label=nucleoplasmin NLS
     misc_feature    12294..12587
                     /label=UGI-NLS
                     /note="UGI-NLS"
     misc_feature    12607..12884
                     /label=NOS term
                     /note="NOS term
                     vntifkey=21
                     ApEinfo_revcolor=green
                     ApEinfo_fwdcolor=#f5010a
                     dnas_title=NOS term
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     protein_bind    12906..12926
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     misc_recomb     12906..12912
                     /label=attR5
                     /note="attR5
                     vntifkey=86
                     ApEinfo_revcolor=pink
                     invitrogen=2703000
                     ApEinfo_fwdcolor=#00ff00
                     dnas_title=attR5
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     misc_feature    12913..12929
                     /label=attL5
                     /note="/dnas_title=attL5"
                     /note="/vntifkey=21"
     misc_feature    12943..12955
                     /label=For Left homology arm
                     /label=nonstandard type: insertion_seq
                     /note="/dnas_title=For Left homology arm"
                     /note="/vntifkey=14"
     promoter        12963..13288
                     /label=AtU3 promoter
                     /note="AtU3 promoter"
     modified_base   13038
                     /label=G to c
                     /note="G to c"
     misc_feature    13224..13233
                     /label=USE
                     /note="USE"
     TATA_signal     13258..13265
                     /note="TATA box"
     misc_feature    13289
                     /label=first necletide transcript
                     /note="first necletide transcript"
     misc_feature    13290..13301
                     /label=FUS_A_left
                     /note="FUS_A_left"
     misc_feature    complement(13317..13592)
                     /label=lacZ'
                     /note="lacZ'"
     promoter        complement(13593..13714)
                     /label=lacZ promoter
                     /note="lacZ promoter"
     misc_structure  13743
                     /label=guide RNA cloning site
                     /note="guide RNA cloning site"
     misc_feature    13744..13819
                     /label=guide RNA scaffold
                     /note="guide RNA scaffold"
     terminator      13820..14001
                     /note="AtU3 terminator"
     protein_bind    complement(14022..14046)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    14022..14031
                     /label=attL2
                     /note="/dnas_title=attL2"
                     /note="/vntifkey=21"
     repeat_region   14032..14046
                     /label=attR2
                     /note="attR2"
     terminator      complement(14100..14339)
                     /label=nos terminator
                     /note="nos terminator"
     terminator      complement(14340..14357)
                     /label=nos terminator(1)
                     /note="nos terminator"
     misc_feature    complement(14386..14449)
                     /label=LacZpromoter
                     /note="LacZpromoter"
     primer_bind     complement(14442..14461)
                     /label=M13-rev
                     /note="M13-rev"
     misc_binding    complement(14467..14489)
                     /label=LacO
                     /note="LacO"
     misc_feature    complement(14679..15459)
                     /label=2X35S Promoter
                     /note="2X35S Promoter"
     misc_feature    complement(15490..16287)
                     /label=nptII (kanR)
                     /note="nptII"
     misc_feature    complement(16325..16528)
                     /label=CaMV 3'UTR(polyA signal)
                     /note="CaMV 3'UTR"
     misc_feature    complement(16595..16620)
                     /label=LeftBorder
                     /note="LeftBorder"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg
     3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
     5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
     5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
     5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
     5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
     6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
     6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
     6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc
     6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
     6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6481 gtaaaacgac ggccagtgcc aagcttggcg tgcctgcagg tcaacatggt ggagcacgac
     6541 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     6601 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     6661 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     6721 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     6781 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     6841 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
     6901 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
     6961 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
     7021 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
     7081 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     7141 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     7201 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     7261 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgccaag
     7321 ctatcaaaca agtttgtaca aaaaagcagg ctccgaattc gcccttcatg gaggcctctc
     7381 cggcttcagg tcctcgccat ctcatggacc cccatatctt tacctccaat ttcaataacg
     7441 gcattggcag gcacaagaca tacctctgtt atgaggtcga gcggctcgat aacggaacct
     7501 cagtgaagat ggaccaacac aggggtttcc ttcataacca agcgaaaaat ctcctttgcg
     7561 gtttctatgg gcggcacgcg gagctgagat tcttggattt ggtcccgagc ctccagctgg
     7621 atcccgcgca aatataccgc gtcacttggt tcatatcatg gagcccgtgc tttagctggg
     7681 ggtgtgccgg tgaggttcgc gcgttcctcc aggaaaacac ccatgttcgc ctccggattt
     7741 tcgcagcgag gattttcgac tatgatccac tttacaaaga ggccctccaa atgcttagag
     7801 atgctggggc tcaagtttca attatgacat acgacgagtt taagcattgt tgggacactt
     7861 ttgtcgatca tcagggttgt cccttccagc cgtgggatgg tcttgatgag cattcgcaag
     7921 ctttgagtgg caggcttcgc gcgatacttc agaaccaggg taactcaggt tctgagacgc
     7981 ctggcacaag tgagtcagca acacccgagt ccaccatgga ttacaaggac cacgacgggg
     8041 attacaagga ccacgacatt gattacaagg atgatgatga caagatggct ccgaagaaga
     8101 agaggaaggt tggcatccac ggggtgccag ctgctgacaa gaagtactcg atcggcctcg
     8161 ctattgggac taactctgtt ggctgggccg tgatcaccga cgagtacaag gtgccctcaa
     8221 agaagttcaa ggtcctgggc aacaccgatc ggcattccat caagaagaat ctcattggcg
     8281 ctctcctgtt cgacagcggc gagacggctg agaggacgcg gctcaagcgc accgcccgca
     8341 ggcggtacac gcgcaggaag aatcgcatct gctacctgca ggagattttc tccaacgaga
     8401 tggcgaaggt tgacgattct ttcttccaca ggctggagga gtcattcctc gtggaggagg
     8461 ataagaagca cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg
     8521 agaagtaccc tacgatctac catctgcgga agaagctcgt ggactccaca gataaggcgg
     8581 acctccgcct gatctacctc gctctggccc acatgattaa gttcaggggc catttcctga
     8641 tcgaggggga tctcaacccg gacaatagcg atgttgacaa gctgttcatc cagctcgtgc
     8701 agacgtacaa ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg
     8761 ctatcctgtc cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc
     8821 cgggcgagaa gaagaacggc ctgttcggga atctcattgc gctcagcctg gggctcacgc
     8881 ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa gctgcagctc tccaaggaca
     8941 catacgacga tgacctggat aacctcctgg cccagatcgg cgatcagtac gcggacctgt
     9001 tcctcgctgc caagaatctg tcggacgcca tcctcctgtc tgatattctc agggtgaaca
     9061 ccgagattac gaaggctccg ctctcagcct ccatgatcaa gcgctacgac gagcaccatc
     9121 aggatctgac cctcctgaag gcgctggtca ggcagcagct ccccgagaag tacaaggaga
     9181 tcttcttcga tcagtcgaag aacggctacg ctgggtacat tgacggcggg gcctctcagg
     9241 aggagttcta caagttcatc aagccgattc tggagaagat ggacggcacg gaggagctgc
     9301 tggtgaagct caatcgcgag gacctcctga ggaagcagcg gacattcgat aacggcagca
     9361 tcccacacca gattcatctc ggggagctgc acgctatcct gaggaggcag gaggacttct
     9421 accctttcct caaggataac cgcgagaaga tcgagaagat tctgactttc aggatcccgt
     9481 actacgtcgg cccactcgct aggggcaact cccgcttcgc ttggatgacc cgcaagtcag
     9541 aggagacgat cacgccgtgg aacttcgagg aggtggtcga caagggcgct agcgctcagt
     9601 cgttcatcga gaggatgacg aatttcgaca agaacctgcc aaatgagaag gtgctcccta
     9661 agcactcgct cctgtacgag tacttcacag tctacaacga gctgactaag gtgaagtatg
     9721 tgaccgaggg catgaggaag ccggctttcc tgtctgggga gcagaagaag gccatcgtgg
     9781 acctcctgtt caagaccaac cggaaggtca cggttaagca gctcaaggag gactacttca
     9841 agaagattga gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct
     9901 ccctggggac ctaccacgat ctcctgaaga tcattaagga taaggacttc ctggacaacg
     9961 aggagaatga ggatatcctc gaggacattg tgctgacact cactctgttc gaggaccggg
    10021 agatgatcga ggagcgcctg aagacttacg cccatctctt cgatgacaag gtcatgaagc
    10081 agctcaagag gaggaggtac accggctggg ggaggctgag caggaagctc atcaacggca
    10141 ttcgggacaa gcagtccggg aagacgatcc tcgacttcct gaagagcgat ggcttcgcga
    10201 accgcaattt catgcagctg attcacgatg acagcctcac attcaaggag gatatccaga
    10261 aggctcaggt gagcggccag ggggactcgc tgcacgagca tatcgcgaac ctcgctggct
    10321 cgccagctat caagaagggg attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg
    10381 tcatgggcag gcacaagcct gagaacatcg tcattgagat ggcccgggag aatcagacca
    10441 cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag gatcgaggag ggcattaagg
    10501 agctggggtc ccagatcctc aaggagcacc cggtggagaa cacgcagctg cagaatgaga
    10561 agctctacct gtactacctc cagaatggcc gcgatatgta tgtggaccag gagctggata
    10621 ttaacaggct cagcgattac gacgtcgatc atatcgttcc acagtcattc ctgaaggatg
    10681 actccattga caacaaggtc ctcaccaggt cggacaagaa ccggggcaag tctgataatg
    10741 ttccttcaga ggaggtcgtt aagaagatga agaactactg gcgccagctc ctgaatgcca
    10801 agctgatcac gcagcggaag ttcgataacc tcacaaaggc tgagaggggc gggctctctg
    10861 agctggacaa ggcgggcttc atcaagaggc agctggtcga gacacggcag atcactaagc
    10921 acgttgcgca gattctcgac tcacggatga acactaagta cgatgagaat gacaagctga
    10981 tccgcgaggt gaaggtcatc accctgaagt caaagctcgt ctccgacttc aggaaggatt
    11041 tccagttcta caaggttcgg gagatcaaca attaccacca tgcccatgac gcgtacctga
    11101 acgcggtggt cggcacagct ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt
    11161 acggggacta caaggtttac gatgtgagga agatgatcgc caagtcggag caggagattg
    11221 gcaaggctac cgccaagtac ttcttctact ctaacattat gaatttcttc aagacagaga
    11281 tcactctggc caatggcgag atccggaagc gccccctcat cgagacgaac ggcgagacgg
    11341 gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc
    11401 cacaagtgaa tatcgtcaag aagacagagg tccagactgg cgggttctct aaggagtcaa
    11461 ttaggcctaa gcggaacagc gacaagctca tcgcccgcaa gaaggactgg gatccgaaga
    11521 agtacggcgg gttcctgtgg cccactgtgg cctactcggt cctggttgtg gcgaaggttg
    11581 agaagggcaa gtccaagaag ctcaagagcg tgaaggagct gctggggatc acgattatgg
    11641 agcgctccag cttcgagaag aacccgatcg atttcctgga ggcgaagggc tacaaggagg
    11701 tgaagaagga cctgatcatt aagctcccca agtactcact cttcgagctg gagaacggca
    11761 ggaagcggat gctggcttcc gctaagcagc tgcagaaggg gaacgagctg gctctgccgt
    11821 ccaagtatgt gaacttcctc tacctggcct cccactacga gaagctcaag ggcagccccg
    11881 aggacaacga gcagaagcag ctgttcgtcg agcagcacaa gcattacctc gacgagatca
    11941 ttgagcagat ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat ctggataagg
    12001 tcctctccgc gtacaacaag caccgcgaca agccaatcag ggagcaggct gagaatatca
    12061 ttcatctctt caccctgacg aggctcggcg cccctagggc tttcaagtac ttcgacacaa
    12121 ctatcgatcc gaagcagtac aggagcacta aggaggtcct ggacgcgacc ctcatccacc
    12181 agtcgattac cggcctctac gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc
    12241 ggccagcggc gacgaagaag gcggggcagg cgaagaagaa gaagggagac ggatcaggcg
    12301 ggtcaacaaa cctcagtgac atcatagaga aggaaactgg taagcaactg gttattcaag
    12361 agagcattct gatgctccct gaggaagtcg aggaagttat aggaaacaag cctgagagcg
    12421 atatactcgt gcatacggcg tatgacgaga gcacggatga aaatgtgatg ctcttgacca
    12481 gcgatgcgcc agaatataaa ccctgggcac tggttattca ggactctaac ggggaaaata
    12541 aaatcaagat gctctcaggt ggctccccaa agaagaaacg caaggtttag tggcggccgc
    12601 cgacgtccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
    12661 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
    12721 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
    12781 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
    12841 tgtcatctat gttactagat cgggaattga tcccccctcg acagcttccg gaaagggcga
    12901 attcgcaact ttgtatacaa aagttgcccc atggcgttcc ctctagataa cgcaggatcc
    12961 tatttacttt aaatttttct tatggctcag cctgtgatgg ataactgaat caaacaaatg
    13021 gcgtctgggt ttaagaacat ctgttttggc tatgttggac gaaacaagtg aacttttagg
    13081 atcaacttcc gtttatatac ggagcttata tcgagcaata agataagtgg gctttttatg
    13141 taatttaatg ggctatcgtc catatattca ctaataccca tgcccagtac ccatgtatgc
    13201 gtttcatata agctcctaat ttctcccaca tcgctcaaat ctaaacaaat cttgttgtat
    13261 atataacact gagggagcac cattggtcag agaccggcgc cgctacaggg cgcgtcccat
    13321 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    13381 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    13441 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
    13501 ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc ttttgttccc tttagtgagg
    13561 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    13621 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    13681 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc accggtggtc
    13741 tctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
    13801 agtggcaccg agtcggtgct tttttttccc tttccttttt tctttttttt gccataaact
    13861 taaatttgta tatcgatcat tgtagatatt gaaaacctag aacaaaccaa catccatgtg
    13921 aatgtctttc atgactgatt tagagataat tcttgaattt tggaactaga atctataatg
    13981 agcctaaatt aaaacattgt gaactagtaa gggcgaattc gacccagctt tcttgtacaa
    14041 agtggttcga taattcttaa ttaactagtt ctagagcggc cgccaccgcg gtggagctcg
    14101 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
    14161 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
    14221 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
    14281 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
    14341 gtgtcatcta tgttactgaa ttcgtaatca tggtcataga agcttgcatg cctgcaggtc
    14401 gactctagag gatccccggg taccgagctc gaattcgtaa tcatgtcata gctgtttcct
    14461 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    14521 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    14581 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    14641 agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg
    14701 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
    14761 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
    14821 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
    14881 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
    14941 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
    15001 ataacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
    15061 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
    15121 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
    15181 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
    15241 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
    15301 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
    15361 cccactatcc ttcgcaagac cttcctctat ataaggaagt tcatttcatt tggagaggac
    15421 acgctgaaat caccagtctc tctctacaaa tctatctctc tcgagctttc gcagatctgt
    15481 cgatcgacca tggggattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
    15541 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
    15601 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
    15661 ctgaatgaac tccaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
    15721 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
    15781 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
    15841 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
    15901 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
    15961 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
    16021 cgcatgcccg acggcgagga tctcgtcgtg acacatggcg atgcctgctt gccgaatatc
    16081 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
    16141 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
    16201 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
    16261 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcggatcg atcctctagc
    16321 tagagtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
    16381 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
    16441 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
    16501 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
    16561 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
//
