LOCUS       pYPQAT25       19235 bp DNA     circular SYN 28-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19235)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19235
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    1119
                     /label=C to G
                     /note="To kill BsaI site"
     rep_origin      1125..1319
                     /label=pVS1 oriV
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas
                     plasmidpVS1 (Heeb et al., 2000)"
     misc_feature    1663..1803
                     /label=bom
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1989..2577)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2664..3458)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5082..5106
                     /label=LB T-DNA repeat
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    5184..5358
                     /label=CaMV poly(A) signal
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5399..6424)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=aph(4)-Ia
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6491..7167)
                     /note="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with
                     aduplicated enhancer region"
     protein_bind    7358..7379
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
     promoter        7394..7424
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7432..7448
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7456..7472
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      7489..7741
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7805..7829
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7805..7812
                     /gene="mutant version of attR"
                     /label=LR Clonase(TM) binding site
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7813..7829
                     /label=attL2
     terminator      complement(7850..8024)
                     /label=AtU3 terminator
                     /note="AtU3 terminator"
     stem_loop       8025..8167
                     /label=guide RNA scaffold 2.0
                     /note="guide RNA scaffold 2.0"
     misc_structure  complement(8168)
                     /label=guide RNA cloning site
                     /note="guide RNA cloning site"
     promoter        8197..8318
                     /label=lacZ promoter
                     /note="lacZ promoter"
     misc_feature    8319..8594
                     /label=lacZ'
                     /note="lacZ'"
     misc_feature    8610..8621
                     /label=FUS_A_left
                     /note="FUS_A_left"
     misc_feature    8622
                     /label=first necletide transcript
                     /note="first necletide transcript"
     promoter        complement(8623..8948)
                     /label=AtU3 promoter
                     /note="AtU3 promoter"
     TATA_signal     8646..8653
                     /note="TATA box"
     misc_feature    8678..8687
                     /label=USE
                     /note="USE"
     modified_base   complement(8873)
                     /label=G to c
                     /note="G to c"
     misc_feature    8982..8991
                     /label=attL5
     protein_bind    complement(8985..9005)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     misc_recomb     8992..9005
                     /label=attR5
                     /note="/ApEinfo_fwdcolor=#00ff00 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=attR5
                     /invitrogen=2703000
                     /vntifkey=86"
     misc_feature    9027..9304
                     /label=NOS term
                     /note="/ApEinfo_fwdcolor=#f5010a 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     /ApEinfo_revcolor=green
                     /dnas_title=NOS term
                     /vntifkey=21"
     misc_feature    9324..9371
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS"
     misc_feature    9378..9671
                     /label=UGI-NLS
                     /note="UGI-NLS"
     misc_feature    9678..9707
                     /label=5X GS linker
                     /note="5X GS linker"
     misc_feature    9708..10097
                     /label=MS2 (At codon optimized)
                     /note="MS2 (At codon optimized)"
     misc_feature    9762..9767
                     /label=BsaI recognize
     gene            complement(10098..10148)
                     /label=N-terminal NLS
                     /note="N-terminal NLS"
     misc_feature    10122..10142
                     /label=SV40 NLS
                     /note="SV40 NLS"
     misc_feature    10149..10202
                     /label=T2A
                     /note="T2A"
     misc_feature    10203..11615
                     /label=GGGS-SH3-3XFLAG-PmCDA1-UGI
                     /note="GGGS-SH3-3XFLAG-PmCDA1-UGI"
     sig_peptide     10689..10709
                     /label=NLS
     CDS             complement(10710..11336)
                     /codon_start=1
                     /label=PmCDA
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKWRSELSIMIQVKILHTTKSPAVG"
     misc_feature    11625..11672
                     /label=nucleoplasmin NLS
     CDS             complement(11673..15773)
                     /codon_start=1
                     /label=zCas9
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    15747..15749
                     /label=D10A
     misc_feature    15798..15818
                     /label=SV40 NLS
     sig_peptide     15825..15890
                     /label=3xFLAG
     protein_bind    complement(15912..15936)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     15912..15921
                     /label=attL1
                     /note="/ApEinfo_fwdcolor=#00ff40 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /dnas_title=attL1
                     /invitrogen=2670000
                     /vntifkey=86"
     protein_bind    complement(15922..15936)
                     /gene="mutant version of attR"
                     /label=LR Clonase(TM) binding site
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(16619..16635)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    16838..16862
                     /label=RB T-DNA repeat
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             18163..18792
                     /codon_start=1
                     /product="stability protein from the Pseudomonas
                     plasmidpVS1 (Heeb et al., 2000)"
                     /label=stability protein from the Pseudomonas plasmid
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
       61 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
      121 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
      181 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
      241 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
      301 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
      361 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
      421 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
      481 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
      541 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
      601 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
      661 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
      721 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
      781 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
      841 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
      901 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
      961 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     1021 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     1081 caaattgccc tagcagggga aaaaggtcga aaaggtctgt ttcctgtgga tagcacgtac
     1141 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     1201 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     1261 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     1321 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     1381 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     1441 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     1501 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     1561 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     1621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     1681 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     1741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1801 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1861 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2401 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     2461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2641 attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt
     2701 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
     2761 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
     2821 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
     2881 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
     2941 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
     3001 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
     3061 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
     3121 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
     3181 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
     3241 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
     3301 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
     3361 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
     3421 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
     3481 tacagtattt aaagataccc caagaagcta aggaaggtgc gaataagcgg ggaaattctt
     3541 ctcggctgac tcagtcattt catttcttca tgtttgagcc gattttttct cccgtaaatg
     3601 ccttgaatca gcctatttag accgtttctt cgccatttaa ggcgttatcc ccagttttta
     3661 gtgagatctc tcccactgac gtatcatttg gtccgcccga aacaggttgg ccagcgtgaa
     3721 taacatcgcc agttggttat cgtttttcag caaccccttg tatctggctt tcacgaagcc
     3781 gaactgtcgc ttgatgatgc gaaatgggtg ctccaccctg gcccggatgc tggctttcat
     3841 gtattcgatg ttgatggccg ttttgttctt gcgtggatgc tgtttcaagg ttcttacctt
     3901 gccggggcgc tcggcgatca gccagtccac atccacctcg gccagctcct cgcgctgtgg
     3961 cgccccttgg tagccggcat cggctgagac aaattgctcc tctccatgca gcagattacc
     4021 cagctgattg aggtcatgct cgttggccgc ggtggtgacc aggctgtggg tcaggccact
     4081 cttggcatcg acaccaatgt gggccttcat gccaaagtgc cactgattgc ctttcttggt
     4141 ctgatgcatc tccggatcgc gttgctgctc tttgttcttg gtcgagctgg gtgcctcaat
     4201 gatggtggca tcgaccaagg tgccttgagt catcatgacg cctgcttcgg ccagccagcg
     4261 attgatggtc ttgaacaatt ggcgggccag ttgatgctgc tccagcaggt ggcggaaatt
     4321 catgatggtg gtgcggtccg gcaaggcgct atccagggat aaccgggcaa acagacgcat
     4381 ggaggcgatt tcgtacagag catcttccat cgcgccatcg ctcaggttgt accaatgctg
     4441 catgcagtga atgcgtagca tggtttccag cggataaggt cgccggccat taccagcctt
     4501 ggggtaaaac ggctcgatga cttccaccat gttttgccat ggcagaatct gctccatgcg
     4561 ggacaagaaa atctcttttc tggtctgacg gcgcttactg ctgaattcac tgtcggcgaa
     4621 ggtaagttga tgactcatga tgaaccctgt tctatggctc cagatgacaa acatgatctc
     4681 atatcaggga cttgttcgca ccttccctaa ttataacaag acgaactcca attcactgtt
     4741 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
     4801 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
     4861 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
     4921 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
     4981 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
     5041 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     5101 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
     5161 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
     5221 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     5281 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
     5341 cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg catctactct
     5401 atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     5461 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     5521 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     5581 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     5641 gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     5701 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     5761 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     5821 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     5881 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     5941 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     6001 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     6061 tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt
     6121 cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc tcgctaaact
     6181 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     6241 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     6301 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     6361 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     6421 tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt gtagagagag
     6481 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
     6541 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     6601 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     6661 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     6721 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     6781 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     6841 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     6901 cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     6961 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     7021 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
     7081 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
     7141 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
     7201 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa
     7261 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7321 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7381 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7441 gataacaatt tcacacagga aacagctatg acatgattac gaattcccga tctagtaaca
     7501 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
     7561 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
     7621 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
     7681 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
     7741 cggggaaatt cgagctccac cgcggtggcg gccgctctag aactagttaa ttaagaatta
     7801 tcgaaccact ttgtacaaga aagctgggtc gaattcgccc ttactagttc acaatgtttt
     7861 aatttaggct cattatagat tctagttcca aaattcaaga attatctcta aatcagtcat
     7921 gaaagacatt cacatggatg ttggtttgtt ctaggttttc aatatctaca atgatcgata
     7981 tacaaattta agtttatggc aaaaaaaaga aaaaaggaaa gggaaaaaaa agcaccgact
     8041 cggtgccact tggccctgca gacatgggtg atcctcatgt tggccaagtt gataacggac
     8101 tagccttatt ttaacttgct aggccctgca gacatgggtg atcctcatgt tggcctagct
     8161 ctaaaacaga gaccaccggt ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8221 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8281 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     8341 cgcaattaac cctcactaaa gggaacaaaa gctggaggac ctcgaggggg ggcccggtac
     8401 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     8461 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8521 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8581 tgaatggcga atgggacgcg ccctgtagcg gcgccggtct ctgaccaatg gtgctccctc
     8641 agtgttatat atacaacaag atttgtttag atttgagcga tgtgggagaa attaggagct
     8701 tatatgaaac gcatacatgg gtactgggca tgggtattag tgaatatatg gacgatagcc
     8761 cattaaatta cataaaaagc ccacttatct tattgctcga tataagctcc gtatataaac
     8821 ggaagttgat cctaaaagtt cacttgtttc gtccaacata gccaaaacag atgttcttaa
     8881 acccagacgc catttgtttg attcagttat ccatcacagg ctgagccata agaaaaattt
     8941 aaagtaaata ggatcctgcg ttatctagag ggaacgccat ggggcaactt ttgtatacaa
     9001 agttgcgaat tcgccctttc cggaagctgt cgagggggga tcaattcccg atctagtaac
     9061 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     9121 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     9181 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     9241 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     9301 tcggacgtcg gcggccgcca ctacttcttt ttcttagcct gtccggcctt tttggtggca
     9361 gcaggacgct tggtaccaac cttgcgtttc ttctttgggg agccacctga gagcatcttg
     9421 attttatttt ccccgttaga gtcctgaata accagtgccc agggtttata ttctggcgca
     9481 tcgctggtca agagcatcac attttcatcc gtgctctcgt catacgccgt atgcacgagt
     9541 atatcgctct caggcttgtt tcctataact tcctcgactt cctcagggag catcagaatg
     9601 ctctcttgaa taaccagttg cttaccagtt tccttctcta tgatgtcact gaggtttgtt
     9661 gacccgcctg aactagttga gccagaaccc gaccccgatc cagagccata gataccgctg
     9721 ttagctgcga tagcggacgg tatagggttt ccatctttca agagtccttg catcgcctta
     9781 acaatcagtt cacaatcaga gtttgtagcg aatattggaa ttgttagctc catgtttaag
     9841 taagatctcc aggctgccac agggagctca acaccaccga ctgtttgtgt tgcaacttta
     9901 gggacttcaa ccttgatggt atattttctt ttttgcgcag aactctgcct aacagaacag
     9961 gtaaccttgt aggcctggga ccttgaattt gagctaatcc actcagcgac tccgttcgca
    10021 aaatttgatg gagctactgt aacgtcgcca gtcccgccgt tatcaaccaa aacaaactgc
    10081 gtgaaatttg acgccatagc cgcaggcacc ccgtgaatac caaccttccg cttcttctta
    10141 ggagccatag ggccgggatt ctcctccacg tcaccgcatg ttagaagact tcctctgccc
    10201 tcgggcacgg ggcaggtagg tctagacagg aagaacctag ctcccctcct ctgacatccg
    10261 ctgcatggag gcatgagagc agcggactgt ctattatggc cagtcatggc gctgtacccc
    10321 cgccatccga ggggccgcag cacttgggta caaccatctt gctctatcat cctggatggt
    10381 cctggattct cttccacatc tccacatgtc agcagggagc cccttccttc aagcatttta
    10441 atcttatttt ctccgttact atcttgtatg accagagccc aaggcttgta ttcaggtgcg
    10501 tccgatgtca gcaacatgac attctcgtcc gtactttcat cgtacgctgt gtgaaccagt
    10561 atgtcagact cgggcttatt tcctataact tcttcgactt cttcgggaag catcaaaatc
    10621 gattcttgga tgaccagttg ctttcctgtt tccttctcga ttatatctga gagattagtc
    10681 atggtcccca ccttgcgctt cttcttcggt cccaccgccg gagatttggt agtatgcagt
    10741 attttaacct ggatcattat cgagagttca ctcctccact tctcggcccg cttcagggtt
    10801 ttttcgagcc acctattctc gttcaattga ttatgtgacg actgaatgaa tatcttccgg
    10861 cagcactggt aatgctccga caccatcaca ttcaacccca cgccgttgtc gcgcaagttc
    10921 cagaggccga tctggttcct tgcatttttc tcgtagtaaa gcttgcaagc ccaaatcttg
    10981 agggtgtgac cgtttcctct gagttcttga ttataccact ccagtatctt ttcagcgcaa
    11041 tctgcacacg gggaccatga agagtaccaa ttgatagtga attgcccggg attatcccga
    11101 aggtattcct cgacctttct aatgctaaat atctccgcgt gaatgcccct ttctgtgccg
    11161 ctttgcggct tgttgacggc atagccccaa aagcaggctc tcctctcacc tctgcgtttc
    11221 aattcgaaca gcacgtaaca gcggtgacta acagattttt tgttgttgaa aaactgtttc
    11281 ttaaacgtgt agatgtcaag tttctcatga atcctgacgt actctgcatc ggtcattccg
    11341 ctcttatcat catcatcctt ataatctata tcgtggtcct tataatctcc atcgtggtct
    11401 ttatagtcac ccgaatactt ctcgacgtag gggacaagaa tcatacctct cttaccttca
    11461 gagtcttcag cgttccacca ttgttcttcc ggcttatctc tgatcctgag aatatctccc
    11521 tttttaaaag gcagatcttc ctcgtcgttt ccattgaagt cgaacaacgc cctgacatac
    11581 tcagcggacc caccccctcc tgtaccgcca cctccgtcgt ctcccttctt cttcttcgcc
    11641 tgccccgcct tcttcgtcgc cgctggccgc ttgtcgcccc cgagctgaga caggtcgatg
    11701 cgcgtctcgt agaggccggt aatcgactgg tggatgaggg tcgcgtccag gacctcctta
    11761 gtgcttgtgt acctcttgcg atcgatagtt gtgtcgaagt acttgaaagc agcaggggcg
    11821 ccgaggttcg tcagggtgaa gagatgaatg atattctcag cctgctccct gattggcttg
    11881 tcgcggtgct tgttgtacgc ggagaggacc ttatccagat tcgcgtcggc caggatcacg
    11941 cgcttggaga actcggaaat ctgctcaatg atctcgtcga ggtaatgctt gtgctgctcg
    12001 acgaacagct gcttctgctc gttgtcctcg gggctgccct tgagcttctc gtagtgggag
    12061 gccaggtaga ggaagttcac atacttggac ggcagagcca gctcgttccc cttctgcagc
    12121 tcgccagcgg aagccagcat ccgcttcctg ccgttctcca gctcgaagag tgagtacttg
    12181 gggagcttaa tgatcaggtc cttcttcacc tccttgtagc ccttcgcctc caggaaatcg
    12241 atcgggttct tctcgaagct ggagcgctcc ataatcgtga tccccagcag ctccttcacg
    12301 ctcttgagct tcttggactt gcccttctca accttcgcca caaccaggac cgagtaggcc
    12361 acagtggggc tgtcgaaccc gccgtacttc ttcggatccc agtccttctt gcgggcgatg
    12421 agcttgtcgc tgttccgctt aggcagaatt gactccttag agaacccgcc agtctggacc
    12481 tctgtcttct tgacgatatt cacttgtggc atggagagaa ccttcctgac ggtcgcgaaa
    12541 tccctgccct tgtcccacac gatctccccc gtctcgccgt tcgtctcgat gagggggcgc
    12601 ttccggatct cgccattggc cagagtgatc tctgtcttga agaaattcat aatgttagag
    12661 tagaagaagt acttggcggt agccttgcca atctcctgct ccgacttggc gatcatcttc
    12721 ctcacatcgt aaaccttgta gtccccgtac acgaactcgc tctcgagctt tgggtacttc
    12781 ttgatcagag ctgtgccgac caccgcgttc aggtacgcgt catgggcatg gtggtaattg
    12841 ttgatctccc gaaccttgta gaactggaaa tccttcctga agtcggagac gagctttgac
    12901 ttcagggtga tgaccttcac ctcgcggatc agcttgtcat tctcatcgta cttagtgttc
    12961 atccgtgagt cgagaatctg cgcaacgtgc ttagtgatct gccgtgtctc gaccagctgc
    13021 ctcttgatga agcccgcctt gtccagctca gagagcccgc ccctctcagc ctttgtgagg
    13081 ttatcgaact tccgctgcgt gatcagcttg gcattcagga gctggcgcca gtagttcttc
    13141 atcttcttaa cgacctcctc tgaaggaaca ttatcagact tgccccggtt cttgtccgac
    13201 ctggtgagga ccttgttgtc aatggagtca tccttcagga atgactgtgg aacgatatga
    13261 tcgacgtcgt aatcgctgag cctgttaata tccagctcct ggtccacata catatcgcgg
    13321 ccattctgga ggtagtacag gtagagcttc tcattctgca gctgcgtgtt ctccaccggg
    13381 tgctccttga ggatctggga ccccagctcc ttaatgccct cctcgatcct cttcatcctc
    13441 tcgcgtgagt tcttctggcc cttctgcgtg gtctgattct cccgggccat ctcaatgacg
    13501 atgttctcag gcttgtgcct gcccatgacc ttcaccagct cgtccacaac cttcacggtc
    13561 tgcagaatcc ccttcttgat agctggcgag ccagcgaggt tcgcgatatg ctcgtgcagc
    13621 gagtccccct ggccgctcac ctgagccttc tggatatcct ccttgaatgt gaggctgtca
    13681 tcgtgaatca gctgcatgaa attgcggttc gcgaagccat cgctcttcag gaagtcgagg
    13741 atcgtcttcc cggactgctt gtcccgaatg ccgttgatga gcttcctgct cagcctcccc
    13801 cagccggtgt acctcctcct cttgagctgc ttcatgacct tgtcatcgaa gagatgggcg
    13861 taagtcttca ggcgctcctc gatcatctcc cggtcctcga acagagtgag tgtcagcaca
    13921 atgtcctcga ggatatcctc attctcctcg ttgtccagga agtccttatc cttaatgatc
    13981 ttcaggagat cgtggtaggt ccccagggag gcgttgaagc ggtcctcaac gccagagatc
    14041 tcgaccgaat cgaagcactc aatcttcttg aagtagtcct ccttgagctg cttaaccgtg
    14101 accttccggt tggtcttgaa caggaggtcc acgatggcct tcttctgctc cccagacagg
    14161 aaagccggct tcctcatgcc ctcggtcaca tacttcacct tagtcagctc gttgtagact
    14221 gtgaagtact cgtacaggag cgagtgctta gggagcacct tctcatttgg caggttcttg
    14281 tcgaaattcg tcatcctctc gatgaacgac tgagcgctag cgcccttgtc gaccacctcc
    14341 tcgaagttcc acggcgtgat cgtctcctct gacttgcggg tcatccaagc gaagcgggag
    14401 ttgcccctag cgagtgggcc gacgtagtac gggatcctga aagtcagaat cttctcgatc
    14461 ttctcgcggt tatccttgag gaaagggtag aagtcctcct gcctcctcag gatagcgtgc
    14521 agctccccga gatgaatctg gtgtgggatg ctgccgttat cgaatgtccg ctgcttcctc
    14581 aggaggtcct cgcgattgag cttcaccagc agctcctccg tgccgtccat cttctccaga
    14641 atcggcttga tgaacttgta gaactcctcc tgagaggccc cgccgtcaat gtacccagcg
    14701 tagccgttct tcgactgatc gaagaagatc tccttgtact tctcggggag ctgctgcctg
    14761 accagcgcct tcaggagggt cagatcctga tggtgctcgt cgtagcgctt gatcatggag
    14821 gctgagagcg gagccttcgt aatctcggtg ttcaccctga gaatatcaga caggaggatg
    14881 gcgtccgaca gattcttggc agcgaggaac aggtccgcgt actgatcgcc gatctgggcc
    14941 aggaggttat ccaggtcatc gtcgtatgtg tccttggaga gctgcagctt ggcgtcctca
    15001 gcgagatcga aattcgactt gaagttgggc gtgagcccca ggctgagcgc aatgagattc
    15061 ccgaacaggc cgttcttctt ctcgcccggc agctgggcga tcaggttctc gaggcgccga
    15121 gacttcgaga gcctagcgga caggatagcc ttcgcgtcga cgcctgacgc attaatgggg
    15181 ttctcctcga agagctggtt gtacgtctgc acgagctgga tgaacagctt gtcaacatcg
    15241 ctattgtccg ggttgagatc cccctcgatc aggaaatggc ccctgaactt aatcatgtgg
    15301 gccagagcga ggtagatcag gcggaggtcc gccttatctg tggagtccac gagcttcttc
    15361 cgcagatggt agatcgtagg gtacttctcg tggtaggcaa cctcgtcgac aatgttgccg
    15421 aagattggat gccgctcgtg cttcttatcc tcctccacga ggaatgactc ctccagcctg
    15481 tggaagaaag aatcgtcaac cttcgccatc tcgttggaga aaatctcctg caggtagcag
    15541 atgcgattct tcctgcgcgt gtaccgcctg cgggcggtgc gcttgagccg cgtagcctca
    15601 gccgtctcgc cgctgtcgaa caggagagcg ccaatgagat tcttcttgat ggaatgccga
    15661 tcggtgttgc ccaggacctt gaacttcttt gagggcacct tgtactcgtc ggtgatcacg
    15721 gcccagccaa cagagttagt cccaatagcg aggccgatcg agtacttctt gtcagcagct
    15781 ggcaccccgt ggatgccaac cttcctcttc ttcttcggag ccatcttgtc atcatcatcc
    15841 ttgtaatcaa tgtcgtggtc cttgtaatcc ccgtcgtggt ccttgtaatc catggtgaag
    15901 ggcgaattcg gagcctgctt ttttgtacaa acttgtttga tagcttggcg cgcctcgagg
    15961 gggggcccgg taccctgtta atcagaaaaa ctcagattaa tctacaaatt cgatcgcaca
    16021 aactagaaac taacaccaga tctagataga aatcacaaat cgaagagtaa ttattcgaca
    16081 aaactcaaat tatttgaaca aatcggatga tatctatgaa accctaatcg agaattaaga
    16141 tgatatctaa cgatcaaacc cagaaaatcg tcttcgatct aagattaaca gaatctaaac
    16201 caaagaacat atacgaaatt gggatcgaac gaaaacaaaa tcgaagattt tgagagaata
    16261 aggaacacag aaatttacct tgatcacggt agagagaatt gagagaaagt ttttaagatt
    16321 ttgagaaatt gaaatctgaa ttgtgaagaa gaagagctct ttgggtattg ttttatagaa
    16381 gaagaagaag aaaagacgag gacgactagg tcacgagaaa gctaaggcgg tgaagcaata
    16441 gctaataata aaatgacacg tgtattgagc gttgtttaca cgcaaagttg tttttggcta
    16501 attgccttat ttttaggttg aggaaaagta tttgtgcttt gagttgataa acacgactcg
    16561 tgtgtgccgg ctgcaaccac tttgacgccg tttattactg actcgtcgaa agcttggcac
    16621 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    16681 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    16741 cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga
    16801 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
    16861 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
    16921 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
    16981 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
    17041 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
    17101 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
    17161 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
    17221 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
    17281 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
    17341 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
    17401 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
    17461 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
    17521 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
    17581 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
    17641 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
    17701 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
    17761 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
    17821 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
    17881 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
    17941 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
    18001 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
    18061 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
    18121 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
    18181 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
    18241 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
    18301 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
    18361 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
    18421 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
    18481 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
    18541 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
    18601 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
    18661 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
    18721 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
    18781 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
    18841 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
    18901 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
    18961 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
    19021 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
    19081 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
    19141 ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc
    19201 cccaagcccg aggaatcggc gtgagcggtc gcaaa
//
