LOCUS       pYPQAT47       19328 bp DNA     circular SYN 06-SEP-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19328)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 6, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19328
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    34..50
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(58..88)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    103..124
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
     primer_bind     complement(141..153)
                     /label=HDV-LacZ-R
     promoter        315..991
                     /note="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with
                     aduplicated enhancer region"
     CDS             1058..2083
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=aph(4)-Ia
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2376..2400
                     /label=LB T-DNA repeat
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4024..4818
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4905..5493
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5679..5819
                     /label=bom
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      6163..6357
                     /label=pVS1 oriV
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas
                     plasmidpVS1 (Heeb et al., 2000)"
     CDS             complement(6423..7496)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas
                     plasmidpVS1 (Heeb et al., 2000)"
                     /label=replication protein from the Pseudomonas plasmi
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7925..8554)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas
                     plasmidpVS1 (Heeb et al., 2000)"
                     /label=stability protein from the Pseudomonas plasmid
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9849..9873
                     /label=RB T-DNA repeat
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10076..10092
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10108..12105
                     /note="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     protein_bind    12148..12172
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    12148..12155
                     /gene="mutant version of attR"
                     /label=LR Clonase(TM) binding site
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     12156..12172
                     /label=attL1
     gene            12191..12241
                     /label=N-terminal NLS
                     /note="N-terminal NLS"
     CDS             12242..15991
                     /codon_start=1
                     /label=Mb2Cpf1
                     /translation="LFQDFTHLYPLSKTVRFELKPIGRTLEHIHAKNFLSQDETMADMY
                     QKVKVILDDYHRDFIADMMGEVKLTKLAEFYDVYLKFRKNPKDDGLQKQLKDLQAVLRK
                     ESVKPIGSGGKYKTGYDRLFGAKLFKDGKELGDLAKFVIAQEGESSPKLAHLAHFEKFS
                     TYFTGFHDNRKNMYSDEDKHTAIAYRLIHENLPRFIDNLQILTTIKQKHSALYDQIINE
                     LTASGLDVSLASHLDGYHKLLTQEGITAYNRIIGEVNGYTNKHNQICHKSERIAKLRPL
                     HKQILSDGMGVSFLPSKFADDSEMCQAVNEFYRHYTDVFAKVQSLFDGFDDHQKDGIYV
                     EHKNLNELSKQAFGDFALLGRVLDGYYVDVVNPEFNERFAKAKTDNAKAKLTKEKDKFI
                     KGVHSLASLEQAIEHHTARHDDESVQAGKLGQYFKHGLAGVDNPIQKIHNNHSTIKGFL
                     ERERPAGERALPKIKSGKNPEMTQLRQLKELLDNALNVAHFAKLLTTKTTLDNQDGNFY
                     GEFGVLYDELAKIPTLYNKVRDYLSQKPFSTEKYKLNFGNPTLLRGWDLNVEKDRFGVI
                     LQKDGCYYLALLDKAHKKVFDNAPNTGKNVYQKMVYKLLPGPNKMLPRVFFAKSNLDYY
                     NPSAELLDKYAKGTHKKGDNFNLKDCHALIDFFKAGINKHPEWQHFGFKFSPTSSYRDL
                     SDFYREVEPQGYQVKFVDINADYIDELVEQGKLYLFQIYNKDFSPKAHGKPNLHTLYFK
                     ALFSEDNLADPIYKLNGEAQIFYRKASLDMNETTIHRAGEVLENKNPDNPKKRQFVYDI
                     IKDKRYTQDKFMLHVPITMNFGVQGMTIKEFNKKVNQSIQQYDEVNVIGIDRGERHLLY
                     LTVINSKGEILEQRSLNDITTASANGTQVTTPYHKILDKREIERLNARVGWGEIETIKE
                     LKSGYLSHVVHQINQLMLKYNAIVVLEDLNFGFKRGRFKVEKQIYQNFENALIKKLNHL
                     VLKDKADDEIGSYKNALQLTNNFTDLKSIGKQTGFLFYVPAWNTSKIDPETGFVDLLKP
                     RYENIAQSQAFFGKFDKICYNTDKGYFEFHIDYAKFTDKAKNSRQKWAICSHGDKRYVY
                     DKTANQNKGAAKGINVNDELKSLFARYHINDKQPNLVMDICQNNDKEFHKSLMCLLKTL
                     LALRYSNASSDEDFILSPVANDEGVFFNSALADDTQPQNADANGAYHIALKGLWLLNEL
                     KNSDDLNKVKLAIDNQTWLNFAQNR"
     misc_feature    13925..13927
                     /label=N563R
     misc_feature    13943..13945
                     /label=K569V
     misc_feature    13955..13957
                     /label=N573R
     misc_feature    14111..14113
                     /label=K625R
     misc_feature    15022
                     /label=C to T
                     /note="To kill BsaI site"
     misc_feature    15992..16039
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS"
     misc_feature    16040..16042
                     /label=stop
                     /note="stop"
     misc_feature    16068..16345
                     /label=NOS term
     protein_bind    16367..16387
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     misc_recomb     16367..16373
                     /label=attR5
     misc_feature    16374..16390
                     /label=attL5
                     /note="/dnas_title=attL5"
                     /note="/vntifkey=21"
     promoter        16408..18370
                     /label=Ubi1 (maize ubiquitin promoter 1)
                     /note="Ubi1 (maize ubiquitin promoter 1)"
     misc_feature    18377..18382
                     /label=1st 6nt complement
                     /note="1st 6nt complement"
     stem_loop       18383..18419
                     /label=Hammerhead ribozyme
                     /note="Hammerhead ribozyme"
     misc_feature    18420..18439
                     /label=FnCpf1 crRNA repeat
     misc_feature    18440..18742
                     /label=LacZ
     promoter        18633..18651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(18699..18717)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    18762..18778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(18786..18816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18831..18852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     stem_loop       18894..18961
                     /label=HDV ribozyme
                     /note="HDV ribozyme"
     protein_bind    complement(18981..19005)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    18981..18990
                     /label=attL2
                     /note="/dnas_title=attL2"
                     /note="/vntifkey=21"
     protein_bind    complement(18991..19005)
                     /gene="mutant version of attR"
                     /label=LR Clonase(TM) binding site
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      19069..19321
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      541 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
      601 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
      661 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
      721 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
      781 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      841 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      901 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      961 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     1021 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     1321 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
     2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
     2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2761 tcttgttata attagggaag gtgcgaacaa gtccctgata tgagatcatg tttgtcatct
     2821 ggagccatag aacagggttc atcatgagtc atcaacttac cttcgccgac agtgaattca
     2881 gcagtaagcg ccgtcagacc agaaaagaga ttttcttgtc ccgcatggag cagattctgc
     2941 catggcaaaa catggtggaa gtcatcgagc cgttttaccc caaggctggt aatggccggc
     3001 gaccttatcc gctggaaacc atgctacgca ttcactgcat gcagcattgg tacaacctga
     3061 gcgatggcgc gatggaagat gctctgtacg aaatcgcctc catgcgtctg tttgcccggt
     3121 tatccctgga tagcgccttg ccggaccgca ccaccatcat gaatttccgc cacctgctgg
     3181 agcagcatca actggcccgc caattgttca agaccatcaa tcgctggctg gccgaagcag
     3241 gcgtcatgat gactcaaggc accttggtcg atgccaccat cattgaggca cccagctcga
     3301 ccaagaacaa agagcagcaa cgcgatccgg agatgcatca gaccaagaaa ggcaatcagt
     3361 ggcactttgg catgaaggcc cacattggtg tcgatgccaa gagtggcctg acccacagcc
     3421 tggtcaccac cgcggccaac gagcatgacc tcaatcagct gggtaatctg ctgcatggag
     3481 aggagcaatt tgtctcagcc gatgccggct accaaggggc gccacagcgc gaggagctgg
     3541 ccgaggtgga tgtggactgg ctgatcgccg agcgccccgg caaggtaaga accttgaaac
     3601 agcatccacg caagaacaaa acggccatca acatcgaata catgaaagcc agcatccggg
     3661 ccagggtgga gcacccattt cgcatcatca agcgacagtt cggcttcgtg aaagccagat
     3721 acaaggggtt gctgaaaaac gataaccaac tggcgatgtt attcacgctg gccaacctgt
     3781 ttcgggcgga ccaaatgata cgtcagtggg agagatctca ctaaaaactg gggataacgc
     3841 cttaaatggc gaagaaacgg tctaaatagg ctgattcaag gcatttacgg gagaaaaaat
     3901 cggctcaaac atgaagaaat gaaatgactg agtcagccga gaagaatttc cccgcttatt
     3961 cgcaccttcc ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
     4021 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
     4081 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
     4141 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
     4201 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
     4261 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
     4321 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
     4381 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
     4441 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
     4501 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
     4561 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
     4621 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
     4681 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
     4741 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
     4801 ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg tgagttttcg
     4861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5041 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     5701 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     5761 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     5821 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5881 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5941 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     6001 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
     6061 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     6121 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     6181 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     6241 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     6301 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     6361 aacagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     6421 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     6481 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
     6541 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
     6601 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
     6661 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
     6721 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
     6781 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
     6841 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
     6901 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
     6961 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
     7021 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
     7081 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
     7141 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
     7201 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
     7261 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
     7321 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
     7381 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
     7441 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
     7501 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc cagccgctta
     7561 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     7621 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     7681 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     7741 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     7801 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     7861 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     7921 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     7981 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     8041 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     8101 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     8161 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     8221 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     8281 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     8341 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     8401 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     8461 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     8521 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     8581 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     8641 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     8701 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     8761 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     8821 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     8881 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct aggcaccgcg ccgcctggcc
     8941 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
     9001 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
     9061 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
     9121 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
     9181 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
     9241 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
     9301 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
     9361 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
     9421 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
     9481 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
     9541 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
     9601 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
     9661 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
     9721 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
     9781 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
     9841 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg
     9901 gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac
     9961 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    10021 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    10081 acgacggcca gtgccaagct tgcatgcctg cagtgcagcg tgacccggtc gtgcccctct
    10141 ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc
    10201 acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga
    10261 ataatataat ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca
    10321 gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac agttttatct
    10381 ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca
    10441 tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt
    10501 tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt
    10561 tagttttttt atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta
    10621 aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga
    10681 taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca
    10741 gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc
    10801 ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt
    10861 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg
    10921 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
    10981 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
    11041 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
    11101 cgtcctcccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatggttag
    11161 ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt
    11221 gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt
    11281 gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga
    11341 tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa
    11401 tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg
    11461 atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattaattc tgtttcaaac
    11521 tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac
    11581 gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt
    11641 ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt
    11701 gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat
    11761 ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg
    11821 gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata
    11881 catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa
    11941 taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag
    12001 cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt
    12061 ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcaggtcga ctctagagga
    12121 tcccctcgag gcgcgccaag ctatcaaaca agtttgtaca aaaaagcagg ctccgaattc
    12181 gcccttcacc atggctccta agaagaagcg gaaggttggt attcacgggg tgcctgcggc
    12241 tctgtttcaa gattttacac atctgtaccc gctgagtaaa acagtgcggt tcgagctgaa
    12301 acccatagga aggaccctcg agcacatcca cgcgaagaat tttctgagcc aggatgaaac
    12361 tatggctgat atgtatcaaa aagttaaggt cattttggac gactatcatc gcgattttat
    12421 tgccgacatg atgggagagg tgaaactcac gaagcttgct gaattttacg acgtctatct
    12481 gaagttcagg aaaaatccta aggacgatgg gctgcaaaaa cagcttaaag accttcaagc
    12541 tgtccttcgg aaggaatcgg tgaagcctat agggtcaggt gggaagtaca aaacaggcta
    12601 cgatagactc tttggggcaa aactcttcaa agatggaaaa gagttgggtg acctcgcaaa
    12661 attcgttata gcccaagaag gtgagtcttc tccgaagctg gctcatcttg ctcattttga
    12721 gaagttcagc acgtatttta ctggatttca cgataatcgg aagaatatgt actcggatga
    12781 agacaagcat actgcaatag cgtacaggct catccatgag aatttgccga gattcatcga
    12841 caatctgcaa atcttgacaa caatcaaaca aaagcatagc gccctctatg atcagataat
    12901 caacgagctc acggcctccg ggctcgacgt ctccttggct tctcatcttg acgggtatca
    12961 caagctcctt acacaagagg ggatcacggc atacaacagg atcataggag aggtgaatgg
    13021 atatacaaat aagcataacc agatatgcca caagagcgag cgcatagcga aacttagacc
    13081 cttgcacaag caaatccttt ctgacggaat gggagtgtca ttccttccgt ctaagttcgc
    13141 ggatgatagt gagatgtgcc aagcggtcaa cgaattttat cgccattata ctgacgtgtt
    13201 cgcaaaggtg caaagtctct ttgacggatt tgatgatcac cagaaagacg ggatctatgt
    13261 tgaacacaaa aaccttaatg aactgagcaa acaggcgttc ggcgactttg ctttgctggg
    13321 gagggtcctt gatggatact acgtggacgt tgtcaatccg gagttcaatg agcggttcgc
    13381 aaaggccaag actgacaatg cgaaagccaa gcttacaaaa gaaaaggaca aattcattaa
    13441 aggagtccac tcactggctt ccctcgaaca agcaatagaa caccatacag ctagacacga
    13501 cgatgagagt gttcaagccg gaaaacttgg ccagtacttc aaacacggtt tggcgggggt
    13561 tgacaacccg attcagaaaa ttcacaataa ccattcgacg attaaagggt ttctggaaag
    13621 ggaaaggcct gctggggaac gggcgctccc gaagatcaag tcaggaaaaa acccagaaat
    13681 gacacagctc aggcagctga aggaactttt ggacaacgca ttgaatgtgg cgcacttcgc
    13741 taagctgctg acaactaaaa caaccttgga caaccaggat ggaaattttt acggggagtt
    13801 tggggtgctt tacgacgagc tggctaaaat tccaactctc tacaataagg ttagagatta
    13861 tctctctcaa aagccctttt ctaccgaaaa gtataagctc aacttcggca atccgaccct
    13921 tctccgcggg tgggacctga acgtggagaa agatcgcttt ggggttatac ttcagaagga
    13981 tggatgctat tacttggcgc ttcttgataa ggctcataaa aaagttttcg acaacgcccc
    14041 taacactggt aagaacgtct accaaaagat ggtctacaaa ctgttgcccg gccccaacaa
    14101 aatgcttcct agggtgtttt tcgcaaaatc gaatctcgac tattataatc catctgccga
    14161 gctccttgac aaatatgcta aggggaccca taaaaagggt gataatttca acctgaagga
    14221 ctgccacgcg cttatcgact ttttcaaagc cgggataaat aagcatccgg agtggcaaca
    14281 ttttggtttt aaattttcgc caacgtcgtc ctatcgcgac ctttccgatt tctataggga
    14341 agttgaacct caggggtacc aggtcaaatt tgttgacatt aatgcggact acattgatga
    14401 attggtggag caagggaagc tctacctctt tcaaatatat aacaaagatt tctcgccaaa
    14461 agcgcatggt aaaccgaatc ttcatacctt gtactttaaa gcactttttt cagaagataa
    14521 cttggcggac ccgatctaca agctgaatgg ggaagctcag atcttctaca ggaaagcttc
    14581 gttggacatg aacgagacta ccatacatcg cgcgggagag gtgcttgaga acaaaaatcc
    14641 cgacaacccg aaaaagcggc aattcgttta cgacatcatc aaagacaaac ggtacacgca
    14701 ggacaaattt atgctccacg tccccattac catgaatttt ggagtccaag gcatgaccat
    14761 taaggaattc aacaaaaagg tcaaccaaag tattcagcaa tacgatgaag tcaatgtcat
    14821 aggcatagat cggggagaaa ggcatctgtt gtatcttacc gtgattaact ctaagggtga
    14881 aatactggag caacggtcac ttaacgatat aaccacggcg tccgcgaacg gtacacaagt
    14941 gaccactccc taccacaaaa tattggataa aagggagata gaacgcttga atgcccgcgt
    15001 tggctggggt gagattgaga cgatcaaaga gcttaaatcg ggatatttgt ctcacgtcgt
    15061 tcatcaaatt aaccaactca tgcttaagta caatgcaatc gttgtgctcg aggacctgaa
    15121 ctttggtttc aaaagaggga ggttcaaggt ggaaaaacaa atttaccaga actttgaaaa
    15181 cgcgcttatc aagaaattga atcaccttgt tttgaaagat aaggcagatg acgaaatcgg
    15241 gtcgtataaa aatgcactcc agttgacaaa taatttcacg gatttgaagt cgatcggcaa
    15301 gcaaacaggg ttcctctttt atgtgccagc gtggaataca tcaaaaattg atccggagac
    15361 gggatttgtc gacttgctga agcctaggta tgagaacatt gcccaatctc aggccttttt
    15421 cggcaaattc gataaaatat gctacaacac agacaaaggt tattttgaat ttcacattga
    15481 ttacgccaaa tttacagata aggcgaaaaa cagcagacag aaatgggcta tctgttctca
    15541 tggggacaaa cgctatgtct acgataagac ggctaatcaa aataaaggcg ccgcaaaagg
    15601 tattaatgtg aatgatgagc tgaaaagctt gtttgcccgc taccatatca atgataaaca
    15661 accaaacttg gtgatggaca tatgccagaa caatgacaaa gaattccaca agtcactcat
    15721 gtgcctgctt aaaacccttt tggcgctgcg gtatagcaat gcatctagcg atgaagactt
    15781 tattttgagt cccgtggcca acgacgaggg cgtgtttttt aattcagcct tggcggacga
    15841 tacgcagccc cagaatgcgg acgcaaacgg cgcgtaccac attgcactga agggactgtg
    15901 gcttctgaac gagctgaaaa atagcgacga cctgaataaa gtcaagttgg ccattgacaa
    15961 tcaaacctgg ttgaatttcg ctcaaaatag aaagcgtcct gctgccacca aaaaggccgg
    16021 acaggctaag aaaaagaagt gagacgacta gtggcggccg ccgacgtccg atcgttcaaa
    16081 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
    16141 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
    16201 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
    16261 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
    16321 tcgggaattg atcccccctc gacagcttcc ggaaagggcg aattcgcaac tttgtataca
    16381 aaagttgccc catggcgttc cctctagaga gataatgagc attgcatgtc taagttataa
    16441 aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata
    16501 catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg
    16561 ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg
    16621 acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa
    16681 atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg
    16741 ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta
    16801 aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag
    16861 aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg
    16921 aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta
    16981 acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg
    17041 catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc
    17101 cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg
    17161 gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc
    17221 gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa
    17281 cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg
    17341 cacctccgct tcaaggtacg ccgctcgtcc tccccccccc ccccctctct accttctcta
    17401 gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt
    17461 agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg
    17521 tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct
    17581 ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt
    17641 ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt
    17701 catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg
    17761 gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt
    17821 gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg
    17881 ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc
    17941 ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa
    18001 tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca
    18061 tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt
    18121 gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct
    18181 aaccttgagt acctatctat tataataaac aagtatgttt tataattatt ttgatcttga
    18241 tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat
    18301 acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt
    18361 acttctgcag ggatccaaat tactgatgag tccgtgagga cgaaacgagt aagctcgtct
    18421 aatttctact gttgtagatg agaccggcgc cgctacaggg cgcgtcccat tcgccattca
    18481 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    18541 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    18601 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
    18661 gtaccgggcc ccccctcgag gtcctccagc ttttgttccc tttagtgagg gttaattgcg
    18721 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    18781 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    18841 taactcacat taattgcgtt gcgctcactg cccgctttcc accggtggtc tctggccggc
    18901 atggtcccag cctcctcgct ggcgccggct gggcaacatg cttcggcatg gcgaatggga
    18961 cggtacccgg ccggaattcg acccagcttt cttgtacaaa gtggttcgat aattcttaat
    19021 taactagttc tagagcggcc gccaccgcgg tggagctcga atttccccga tcgttcaaac
    19081 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
    19141 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
    19201 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
    19261 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
    19321 cgggaatt
//
