LOCUS       pYPQAT48       15605 bp DNA     circular SYN 06-SEP-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15605)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 6, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15605
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    2301..2306
                     /label=Esp3I recognition site(1)
                     /note="Esp3I recognition site"
     primer_bind     complement(2737..2765)
                     /label=Alldest-mod-F
     misc_feature    2764
                     /label=G to C
                     /note="To kill BsaI site"
     misc_feature    complement(5150..5155)
                     /label=Esp3I recognition site
                     /note="Esp3I recognition site"
     misc_feature    complement(6234..6259)
                     /label=RightBorder
                     /note="RightBorder"
     misc_feature    complement(6338..6500)
                     /label=LacZpromoter(1)
                     /note="LacZpromoter"
     promoter        6519..7287
                     /label=2X35S promoter
                     /note="2X35S promoter"
     repeat_region   7323..7335
                     /label=attR1
                     /note="attR1"
     protein_bind    7328..7352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     7336..7352
                     /label=attL1
     gene            7371..7421
                     /label=N-terminal NLS
                     /note="N-terminal NLS"
     CDS             7422..11171
                     /codon_start=1
                     /label=Mb2Cpf1
                     /translation="LFQDFTHLYPLSKTVRFELKPIGRTLEHIHAKNFLSQDETMADMY
                     QKVKVILDDYHRDFIADMMGEVKLTKLAEFYDVYLKFRKNPKDDGLQKQLKDLQAVLRK
                     ESVKPIGSGGKYKTGYDRLFGAKLFKDGKELGDLAKFVIAQEGESSPKLAHLAHFEKFS
                     TYFTGFHDNRKNMYSDEDKHTAIAYRLIHENLPRFIDNLQILTTIKQKHSALYDQIINE
                     LTASGLDVSLASHLDGYHKLLTQEGITAYNRIIGEVNGYTNKHNQICHKSERIAKLRPL
                     HKQILSDGMGVSFLPSKFADDSEMCQAVNEFYRHYTDVFAKVQSLFDGFDDHQKDGIYV
                     EHKNLNELSKQAFGDFALLGRVLDGYYVDVVNPEFNERFAKAKTDNAKAKLTKEKDKFI
                     KGVHSLASLEQAIEHHTARHDDESVQAGKLGQYFKHGLAGVDNPIQKIHNNHSTIKGFL
                     ERERPAGERALPKIKSGKNPEMTQLRQLKELLDNALNVAHFAKLLTTKTTLDNQDGNFY
                     GEFGVLYDELAKIPTLYNKVRDYLSQKPFSTEKYKLNFGNPTLLRGWDLNVEKDRFGVI
                     LQKDGCYYLALLDKAHKKVFDNAPNTGKNVYQKMVYKLLPGPNKMLPRVFFAKSNLDYY
                     NPSAELLDKYAKGTHKKGDNFNLKDCHALIDFFKAGINKHPEWQHFGFKFSPTSSYRDL
                     SDFYREVEPQGYQVKFVDINADYIDELVEQGKLYLFQIYNKDFSPKAHGKPNLHTLYFK
                     ALFSEDNLADPIYKLNGEAQIFYRKASLDMNETTIHRAGEVLENKNPDNPKKRQFVYDI
                     IKDKRYTQDKFMLHVPITMNFGVQGMTIKEFNKKVNQSIQQYDEVNVIGIDRGERHLLY
                     LTVINSKGEILEQRSLNDITTASANGTQVTTPYHKILDKREIERLNARVGWGEIETIKE
                     LKSGYLSHVVHQINQLMLKYNAIVVLEDLNFGFKRGRFKVEKQIYQNFENALIKKLNHL
                     VLKDKADDEIGSYKNALQLTNNFTDLKSIGKQTGFLFYVPAWNTSKIDPETGFVDLLKP
                     RYENIAQSQAFFGKFDKICYNTDKGYFEFHIDYAKFTDKAKNSRQKWAICSHGDKRYVY
                     DKTANQNKGAAKGINVNDELKSLFARYHINDKQPNLVMDICQNNDKEFHKSLMCLLKTL
                     LALRYSNASSDEDFILSPVANDEGVFFNSALADDTQPQNADANGAYHIALKGLWLLNEL
                     KNSDDLNKVKLAIDNQTWLNFAQNR"
     misc_feature    9105..9107
                     /label=N563R
     misc_feature    9123..9125
                     /label=K569V
     misc_feature    9135..9137
                     /label=N573R
     misc_feature    9291..9293
                     /label=K625R
     misc_feature    10202
                     /label=C to T
                     /note="To kill BsaI site"
     misc_feature    11172..11219
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS"
     misc_feature    11220..11222
                     /label=stop
                     /note="stop"
     misc_feature    11248..11525
                     /label=NOS term
     protein_bind    11547..11567
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     misc_recomb     11547..11553
                     /label=attR5
     misc_feature    11554..11570
                     /label=attL5
     promoter        11601..12369
                     /label=2X35S promoter
     misc_feature    12403..12408
                     /label=1st 6nt complement
                     /note="1st 6nt complement"
     stem_loop       12409..12445
                     /label=Hammerhead ribozyme
                     /note="Hammerhead ribozyme"
     misc_feature    12446..12460
                     /label=FnCpf1 crRNA repeat
                     /note="ApEinfo_fwdcolor=#00ffff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=green"
     promoter        12659..12677
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(12725..12743)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    12788..12804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12812..12842)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12857..12878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     stem_loop       12920..12987
                     /label=HDV ribozyme
                     /note="HDV ribozyme"
     protein_bind    complement(13007..13031)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    13007..13016
                     /label=attL2
     repeat_region   13017..13031
                     /label=attR2
                     /note="attR2"
     terminator      complement(13085..13324)
                     /label=nos terminator
                     /note="nos terminator"
     terminator      complement(13325..13342)
                     /label=nos terminator(1)
                     /note="nos terminator"
     misc_feature    complement(13371..13434)
                     /label=LacZpromoter
                     /note="LacZpromoter"
     misc_binding    complement(13452..13474)
                     /label=LacO
                     /note="LacO"
     misc_feature    complement(13664..14444)
                     /label=2X35S Promoter
                     /note="2X35S Promoter"
     misc_feature    complement(14475..15272)
                     /label=nptII (kanR)
                     /note="nptII"
     misc_feature    complement(15310..15513)
                     /label=CaMV 3'UTR(polyA signal)
                     /note="CaMV 3'UTR"
     misc_feature    complement(15580..15605)
                     /label=LeftBorder
                     /note="LeftBorder"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg
     3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
     5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
     5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
     5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
     5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
     6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
     6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
     6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc
     6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
     6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6481 gtaaaacgac ggccagtgcc aagcttggcg tgcctgcagg tcaacatggt ggagcacgac
     6541 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     6601 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     6661 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     6721 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     6781 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     6841 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
     6901 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
     6961 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
     7021 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
     7081 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     7141 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     7201 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     7261 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgccaag
     7321 ctatcaaaca agtttgtaca aaaaagcagg ctccgaattc gcccttcacc atggctccta
     7381 agaagaagcg gaaggttggt attcacgggg tgcctgcggc tctgtttcaa gattttacac
     7441 atctgtaccc gctgagtaaa acagtgcggt tcgagctgaa acccatagga aggaccctcg
     7501 agcacatcca cgcgaagaat tttctgagcc aggatgaaac tatggctgat atgtatcaaa
     7561 aagttaaggt cattttggac gactatcatc gcgattttat tgccgacatg atgggagagg
     7621 tgaaactcac gaagcttgct gaattttacg acgtctatct gaagttcagg aaaaatccta
     7681 aggacgatgg gctgcaaaaa cagcttaaag accttcaagc tgtccttcgg aaggaatcgg
     7741 tgaagcctat agggtcaggt gggaagtaca aaacaggcta cgatagactc tttggggcaa
     7801 aactcttcaa agatggaaaa gagttgggtg acctcgcaaa attcgttata gcccaagaag
     7861 gtgagtcttc tccgaagctg gctcatcttg ctcattttga gaagttcagc acgtatttta
     7921 ctggatttca cgataatcgg aagaatatgt actcggatga agacaagcat actgcaatag
     7981 cgtacaggct catccatgag aatttgccga gattcatcga caatctgcaa atcttgacaa
     8041 caatcaaaca aaagcatagc gccctctatg atcagataat caacgagctc acggcctccg
     8101 ggctcgacgt ctccttggct tctcatcttg acgggtatca caagctcctt acacaagagg
     8161 ggatcacggc atacaacagg atcataggag aggtgaatgg atatacaaat aagcataacc
     8221 agatatgcca caagagcgag cgcatagcga aacttagacc cttgcacaag caaatccttt
     8281 ctgacggaat gggagtgtca ttccttccgt ctaagttcgc ggatgatagt gagatgtgcc
     8341 aagcggtcaa cgaattttat cgccattata ctgacgtgtt cgcaaaggtg caaagtctct
     8401 ttgacggatt tgatgatcac cagaaagacg ggatctatgt tgaacacaaa aaccttaatg
     8461 aactgagcaa acaggcgttc ggcgactttg ctttgctggg gagggtcctt gatggatact
     8521 acgtggacgt tgtcaatccg gagttcaatg agcggttcgc aaaggccaag actgacaatg
     8581 cgaaagccaa gcttacaaaa gaaaaggaca aattcattaa aggagtccac tcactggctt
     8641 ccctcgaaca agcaatagaa caccatacag ctagacacga cgatgagagt gttcaagccg
     8701 gaaaacttgg ccagtacttc aaacacggtt tggcgggggt tgacaacccg attcagaaaa
     8761 ttcacaataa ccattcgacg attaaagggt ttctggaaag ggaaaggcct gctggggaac
     8821 gggcgctccc gaagatcaag tcaggaaaaa acccagaaat gacacagctc aggcagctga
     8881 aggaactttt ggacaacgca ttgaatgtgg cgcacttcgc taagctgctg acaactaaaa
     8941 caaccttgga caaccaggat ggaaattttt acggggagtt tggggtgctt tacgacgagc
     9001 tggctaaaat tccaactctc tacaataagg ttagagatta tctctctcaa aagccctttt
     9061 ctaccgaaaa gtataagctc aacttcggca atccgaccct tctccgcggg tgggacctga
     9121 acgtggagaa agatcgcttt ggggttatac ttcagaagga tggatgctat tacttggcgc
     9181 ttcttgataa ggctcataaa aaagttttcg acaacgcccc taacactggt aagaacgtct
     9241 accaaaagat ggtctacaaa ctgttgcccg gccccaacaa aatgcttcct agggtgtttt
     9301 tcgcaaaatc gaatctcgac tattataatc catctgccga gctccttgac aaatatgcta
     9361 aggggaccca taaaaagggt gataatttca acctgaagga ctgccacgcg cttatcgact
     9421 ttttcaaagc cgggataaat aagcatccgg agtggcaaca ttttggtttt aaattttcgc
     9481 caacgtcgtc ctatcgcgac ctttccgatt tctataggga agttgaacct caggggtacc
     9541 aggtcaaatt tgttgacatt aatgcggact acattgatga attggtggag caagggaagc
     9601 tctacctctt tcaaatatat aacaaagatt tctcgccaaa agcgcatggt aaaccgaatc
     9661 ttcatacctt gtactttaaa gcactttttt cagaagataa cttggcggac ccgatctaca
     9721 agctgaatgg ggaagctcag atcttctaca ggaaagcttc gttggacatg aacgagacta
     9781 ccatacatcg cgcgggagag gtgcttgaga acaaaaatcc cgacaacccg aaaaagcggc
     9841 aattcgttta cgacatcatc aaagacaaac ggtacacgca ggacaaattt atgctccacg
     9901 tccccattac catgaatttt ggagtccaag gcatgaccat taaggaattc aacaaaaagg
     9961 tcaaccaaag tattcagcaa tacgatgaag tcaatgtcat aggcatagat cggggagaaa
    10021 ggcatctgtt gtatcttacc gtgattaact ctaagggtga aatactggag caacggtcac
    10081 ttaacgatat aaccacggcg tccgcgaacg gtacacaagt gaccactccc taccacaaaa
    10141 tattggataa aagggagata gaacgcttga atgcccgcgt tggctggggt gagattgaga
    10201 cgatcaaaga gcttaaatcg ggatatttgt ctcacgtcgt tcatcaaatt aaccaactca
    10261 tgcttaagta caatgcaatc gttgtgctcg aggacctgaa ctttggtttc aaaagaggga
    10321 ggttcaaggt ggaaaaacaa atttaccaga actttgaaaa cgcgcttatc aagaaattga
    10381 atcaccttgt tttgaaagat aaggcagatg acgaaatcgg gtcgtataaa aatgcactcc
    10441 agttgacaaa taatttcacg gatttgaagt cgatcggcaa gcaaacaggg ttcctctttt
    10501 atgtgccagc gtggaataca tcaaaaattg atccggagac gggatttgtc gacttgctga
    10561 agcctaggta tgagaacatt gcccaatctc aggccttttt cggcaaattc gataaaatat
    10621 gctacaacac agacaaaggt tattttgaat ttcacattga ttacgccaaa tttacagata
    10681 aggcgaaaaa cagcagacag aaatgggcta tctgttctca tggggacaaa cgctatgtct
    10741 acgataagac ggctaatcaa aataaaggcg ccgcaaaagg tattaatgtg aatgatgagc
    10801 tgaaaagctt gtttgcccgc taccatatca atgataaaca accaaacttg gtgatggaca
    10861 tatgccagaa caatgacaaa gaattccaca agtcactcat gtgcctgctt aaaacccttt
    10921 tggcgctgcg gtatagcaat gcatctagcg atgaagactt tattttgagt cccgtggcca
    10981 acgacgaggg cgtgtttttt aattcagcct tggcggacga tacgcagccc cagaatgcgg
    11041 acgcaaacgg cgcgtaccac attgcactga agggactgtg gcttctgaac gagctgaaaa
    11101 atagcgacga cctgaataaa gtcaagttgg ccattgacaa tcaaacctgg ttgaatttcg
    11161 ctcaaaatag aaagcgtcct gctgccacca aaaaggccgg acaggctaag aaaaagaagt
    11221 gagacgacta gtggcggccg ccgacgtccg atcgttcaaa catttggcaa taaagtttct
    11281 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
    11341 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
    11401 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
    11461 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg atcccccctc
    11521 gacagcttcc ggaaagggcg aattcgcaac tttgtataca aaagttgccc catggcgttc
    11581 cctctagtgg cgtgcctgca ggtcaacatg gtggagcacg acacacttgt ctactccaaa
    11641 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta
    11701 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
    11761 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
    11821 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
    11881 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg
    11941 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
    12001 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
    12061 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
    12121 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
    12181 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
    12241 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
    12301 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac ctcgactcta
    12361 gaggatcccc gggtacgttt ggtgttactt ctgcagggat ccaaattact gatgagtccg
    12421 tgaggacgaa acgagtaagc tcgtctaatt tctactgttg tagatgagac cggcgccgct
    12481 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    12541 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    12601 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
    12661 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcc tccagctttt
    12721 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
    12781 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    12841 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    12901 ctttccaccg gtggtctctg gccggcatgg tcccagcctc ctcgctggcg ccggctgggc
    12961 aacatgcttc ggcatggcga atgggacggt acccggccgg aattcgaccc agctttcttg
    13021 tacaaagtgg ttcgataatt cttaattaac tagttctaga gcggccgcca ccgcggtgga
    13081 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
    13141 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
    13201 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
    13261 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
    13321 gcgcggtgtc atctatgtta ctgaattcgt aatcatggtc atagaagctt gcatgcctgc
    13381 aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt
    13441 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    13501 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    13561 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    13621 cggggagagg cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac
    13681 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
    13741 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
    13801 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
    13861 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
    13921 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
    13981 atgtgataac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
    14041 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
    14101 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
    14161 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
    14221 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
    14281 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
    14341 acaatcccac tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag
    14401 aggacacgct gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga
    14461 tctgtcgatc gaccatgggg attgaacaag atggattgca cgcaggttct ccggccgctt
    14521 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
    14581 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
    14641 gtgccctgaa tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
    14701 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
    14761 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
    14821 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
    14881 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
    14941 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
    15001 aggcgcgcat gcccgacggc gaggatctcg tcgtgacaca tggcgatgcc tgcttgccga
    15061 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
    15121 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
    15181 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
    15241 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg gatcgatcct
    15301 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
    15361 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
    15421 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
    15481 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
    15541 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
    15601 tgcca
//
