LOCUS       pYPQAT51       15537 bp DNA     circular SYN 06-SEP-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15537)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 6, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15537
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    2301..2306
                     /label=Esp3I recognition site(1)
                     /note="Esp3I recognition site"
     misc_feature    2764
                     /label=G to C
                     /note="To kill BsaI site"
     misc_feature    complement(5150..5155)
                     /label=Esp3I recognition site
                     /note="Esp3I recognition site"
     misc_feature    complement(6234..6259)
                     /label=RightBorder
                     /note="RightBorder"
     misc_feature    complement(6338..6500)
                     /label=LacZpromoter(1)
                     /note="LacZpromoter"
     promoter        6519..7287
                     /label=2X35S promoter
                     /note="2X35S promoter"
     repeat_region   7323..7335
                     /label=attR1
                     /note="attR1"
     protein_bind    7328..7352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     7336..7352
                     /label=attL1
     gene            7371..7421
                     /label=N-terminal NLS
                     /note="N-terminal NLS"
     gene            7422..11102
                     /label=LbCpf1
                     /note="LbCpf1"
     misc_feature    7854..7856
                     /label=G146R
     misc_feature    7962..7964
                     /label=R182V
     misc_feature    9801..9803
                     /label=CAG
     misc_difference 9801..9803
                     /label=E795Q
     misc_feature    11103..11150
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS"
     misc_feature    11151..11153
                     /label=stop
                     /note="stop"
     misc_feature    11179..11456
                     /label=NOS term
     protein_bind    11478..11498
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     misc_recomb     11478..11484
                     /label=attR5
     misc_feature    11485..11501
                     /label=attL5
     promoter        11532..12300
                     /label=2X35S promoter
     misc_feature    12334..12339
                     /label=1st 6nt complement
                     /note="1st 6nt complement"
     stem_loop       12340..12376
                     /label=Hammerhead ribozyme
                     /note="Hammerhead ribozyme"
     repeat_region   12377..12392
                     /label=LbCpf1 crRNA repeat
                     /note="LbCpf1 crRNA repeat"
     promoter        12591..12609
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(12657..12675)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    12720..12736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12744..12774)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12789..12810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     stem_loop       12852..12919
                     /label=HDV ribozyme
                     /note="HDV ribozyme"
     protein_bind    complement(12939..12963)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    12939..12948
                     /label=attL2
     repeat_region   12949..12963
                     /label=attR2
                     /note="attR2"
     terminator      complement(13017..13256)
                     /label=nos terminator
                     /note="nos terminator"
     terminator      complement(13257..13274)
                     /label=nos terminator(1)
                     /note="nos terminator"
     misc_feature    complement(13303..13366)
                     /label=LacZpromoter
                     /note="LacZpromoter"
     misc_binding    complement(13384..13406)
                     /label=LacO
                     /note="LacO"
     misc_feature    complement(13596..14376)
                     /label=2X35S Promoter
                     /note="2X35S Promoter"
     misc_feature    complement(14407..15204)
                     /label=nptII (kanR)
                     /note="nptII"
     misc_feature    complement(15242..15445)
                     /label=CaMV 3'UTR(polyA signal)
                     /note="CaMV 3'UTR"
     misc_feature    complement(15512..15537)
                     /label=LeftBorder
                     /note="LeftBorder"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg
     3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
     5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
     5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
     5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
     5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
     6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
     6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
     6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc
     6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
     6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6481 gtaaaacgac ggccagtgcc aagcttggcg tgcctgcagg tcaacatggt ggagcacgac
     6541 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     6601 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     6661 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     6721 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     6781 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     6841 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
     6901 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
     6961 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
     7021 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
     7081 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     7141 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     7201 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     7261 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgccaag
     7321 ctatcaaaca agtttgtaca aaaaagcagg ctccgaattc gcccttcacc atggctccta
     7381 agaagaagcg gaaggttggt attcacgggg tgcctgcggc ttcaaagctc gagaaattca
     7441 ccaactgtta ttcgttgagc aaaacactgc ggtttaaagc gattccagtc ggcaagactc
     7501 aagagaatat agacaataag cggctgttgg tggaagatga aaagcgcgcg gaagactaca
     7561 aaggggtgaa gaagttgttg gacagatact acctctcttt tatcaatgat gtcttgcact
     7621 caatcaaatt gaagaatctg aacaactaca tctccctctt cagaaagaaa acaaggacag
     7681 aaaaggagaa taaggaactt gaaaatttgg agatcaatct gaggaaagag atcgcgaaag
     7741 cctttaaagg caacgaagga tacaaaagtc tgttcaagaa ggatataatt gagacaattt
     7801 tgccagagtt cctcgatgac aaggacgaga ttgcgctggt caattcgttc aaccgcttca
     7861 caacagcatt cacaggcttc tttcgcaatc gggaaaatat gttctctgag gaggcaaagt
     7921 ccacttctat tgcgttcagg tgtatcaatg agaatctcac tgtgtacatt tccaacatgg
     7981 atatctttga gaaggttgac gcaatttttg acaagcacga agttcaggag attaaggaga
     8041 agatcctcaa ttccgattat gacgttgagg acttcttcga aggtgagttt tttaatttcg
     8101 tgctcactca agagggtatc gacgtgtata atgcgatcat cggtgggttc gtgactgagt
     8161 ccggtgaaaa gattaaggga ttgaacgagt atatcaacct ttacaaccaa aagacgaaac
     8221 agaagctgcc aaagttcaag cctctttaca aacaggttct ttcagaccgc gagtcactct
     8281 cgttctatgg ggagggctac acttcggatg aggaagtcct ggaggtgttc aggaatactc
     8341 tcaataagaa ttcggagatt ttctcttcta taaaaaaact ggaaaagttg tttaagaatt
     8401 ttgacgaata ctctagcgcc ggcatatttg tgaaaaacgg cccggccata tcaacgataa
     8461 gtaaagatat cttcggcgaa tggaacgtga tcagagacaa atggaacgcg gagtatgacg
     8521 atattcacct gaagaagaag gctgtcgtaa cggagaagta cgaggatgat cgcaggaaaa
     8581 gcttcaaaaa gatcggaagt ttcagcctgg aacagttgca ggagtatgct gacgccgatc
     8641 ttagcgtcgt cgagaagttg aaggagataa tcatccaaaa ggtcgacgag atatataaag
     8701 tctatggatc aagtgaaaaa ctgttcgacg ccgacttcgt tttggagaag tccctgaaga
     8761 agaacgacgc tgttgttgcc attatgaagg atctgctcga cagcgtgaag agtttcgaga
     8821 actatattaa ggcttttttc ggggagggga aggagactaa cagagatgag tccttctacg
     8881 gagacttcgt cctcgcgtac gatatactcc ttaaggtaga ccacatctac gacgcaatca
     8941 gaaattacgt gacacaaaag ccgtacagca aggacaagtt caaactctac ttccagaacc
     9001 cccagttcat gggcggctgg gacaaggaca aggaaacgga ttacagggct acgatcctga
     9061 ggtatggttc aaaatactac ttggcgatta tggacaagaa gtacgccaag tgtctccaga
     9121 agattgacaa agacgatgtc aatggcaatt atgagaagat caactacaag ctgcttccgg
     9181 gtccgaacaa gatgctccca aaggttttct tcagcaagaa atggatggcc tactataacc
     9241 caagcgagga catccagaag atttataaga acggtacgtt caagaagggc gacatgttca
     9301 atcttaacga ctgtcacaag ctgatcgact tcttcaaaga ctcaattagc cggtacccaa
     9361 agtggtctaa cgcctatgac ttcaactttt cggaaaccga gaagtacaag gatatagccg
     9421 gattttatag agaggtggaa gagcagggct acaaggtgtc attcgagtcc gccagcaaga
     9481 aggaagtgga caagctcgtg gaagagggta agctctacat gttccagatt tataataaag
     9541 actttagcga taagagccac gggacaccta atctccacac aatgtatttc aagctgctct
     9601 tcgacgagaa taaccacggc caaatcaggt tgtcaggagg ggctgaactc ttcatgcggc
     9661 gcgctagcct taagaaggag gagcttgtag tccaccctgc gaatagtcca attgcgaata
     9721 agaacccgga caatcctaaa aagactacaa cattgagcta cgacgtgtac aaggataaga
     9781 ggttttccga ggatcagtac gagctccaca tcccgattgc gatcaacaag tgcccaaaga
     9841 atattttcaa gataaacaca gaggtgcgtg tactcctgaa gcatgacgac aatccttacg
     9901 tcattgggat tgatcggggc gagaggaacc tcctctatat tgtggtggtg gacgggaagg
     9961 ggaacatagt cgaacagtac tcccttaacg aaataattaa caatttcaac ggcatccgta
    10021 tcaagaccga ctaccattcg ttgctggaca agaaggagaa ggagagattt gaggcgcggc
    10081 aaaattggac aagtatcgag aacatcaagg aactcaaagc aggttatatc tctcaagttg
    10141 tgcataagat atgcgagctg gttgagaagt atgacgcagt gatcgctctt gaggacctca
    10201 actcgggctt taagaattct agagttaaag tggagaagca ggtctatcaa aagttcgaga
    10261 agatgcttat agataagctc aactacatgg tcgataagaa atcgaaccca tgtgccaccg
    10321 gcggcgcact caaaggttac caaataacaa acaaattcga gtccttcaaa tcgatgagta
    10381 ctcagaatgg gttcatattt tatataccgg cgtggcttac gtctaagatc gacccgtcaa
    10441 ctggttttgt caacctgttg aagacgaaat acacgtccat tgccgattcg aaaaagttca
    10501 tatctagttt tgatcgtatt atgtacgtcc cagaggaaga tcttttcgag tttgctctcg
    10561 actacaaaaa cttttcgcgg accgatgcgg attacattaa aaaatggaaa ctctattcgt
    10621 acggcaacag aatcaggatt tttcgcaacc ctaagaagaa taacgtcttt gattgggagg
    10681 aagtttgctt gactagcgcg tacaaggagc tctttaataa gtatggcatt aactaccaac
    10741 agggtgatat cagagcactg ctttgcgaac aatctgacaa ggctttctac tcatccttca
    10801 tggctttgat gagcctgatg ctccagatga gaaattcaat tacaggcaga accgacgtgg
    10861 atttcttgat ctccccggtt aaaaattctg atggcatctt ttacgatagc aggaactatg
    10921 aagcgcaaga gaatgcgatt ctgccaaaaa atgcagacgc caacggtgcc tataacatcg
    10981 ccaggaaagt cctgtgggcg atcggccagt tcaaaaaggc cgaagacgaa aaattggaca
    11041 aggtcaaaat cgctatcagc aacaaagagt ggctggagta tgctcagaca tccgtaaagc
    11101 ataagcgtcc tgctgccacc aaaaaggccg gacaggctaa gaaaaagaag tgagacgact
    11161 agtggcggcc gccgacgtcc gatcgttcaa acatttggca ataaagtttc ttaagattga
    11221 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
    11281 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
    11341 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
    11401 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt gatcccccct cgacagcttc
    11461 cggaaagggc gaattcgcaa ctttgtatac aaaagttgcc ccatggcgtt ccctctagtg
    11521 gcgtgcctgc aggtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa
    11581 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
    11641 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
    11701 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
    11761 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
    11821 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg ataacatggt ggagcacgac
    11881 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
    11941 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
    12001 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
    12061 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
    12121 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    12181 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
    12241 ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgactct agaggatccc
    12301 cgggtacgtt tggtgttact tctgcaggga tccaaattac tgatgagtcc gtgaggacga
    12361 aacgagtaag ctcgtctaat ttctactaag tgtagatgag accggcgccg ctacagggcg
    12421 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    12481 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    12541 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
    12601 cactataggg cgaattgggt accgggcccc ccctcgaggt cctccagctt ttgttccctt
    12661 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    12721 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    12781 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccac
    12841 cggtggtctc tggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct
    12901 tcggcatggc gaatgggacg gtacccggcc ggaattcgac ccagctttct tgtacaaagt
    12961 ggttcgataa ttcttaatta actagttcta gagcggccgc caccgcggtg gagctcgaat
    13021 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
    13081 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
    13141 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
    13201 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
    13261 tcatctatgt tactgaattc gtaatcatgg tcatagaagc ttgcatgcct gcaggtcgac
    13321 tctagaggat ccccgggtac cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg
    13381 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    13441 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    13501 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    13561 ggcggtttgc gtattggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct
    13621 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
    13681 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
    13741 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
    13801 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
    13861 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
    13921 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
    13981 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
    14041 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
    14101 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
    14161 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
    14221 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
    14281 actatccttc gcaagacctt cctctatata aggaagttca tttcatttgg agaggacacg
    14341 ctgaaatcac cagtctctct ctacaaatct atctctctcg agctttcgca gatctgtcga
    14401 tcgaccatgg ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
    14461 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
    14521 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
    14581 aatgaactcc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
    14641 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
    14701 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
    14761 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
    14821 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
    14881 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
    14941 atgcccgacg gcgaggatct cgtcgtgaca catggcgatg cctgcttgcc gaatatcatg
    15001 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
    15061 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
    15121 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
    15181 cgccttcttg acgagttctt ctgagcggga ctctggggtt cggatcgatc ctctagctag
    15241 agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
    15301 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
    15361 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
    15421 actaaaatcc agatcccccg aattaattcg gcgttaattc agtacattaa aaacgtccgc
    15481 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgcca
//
