LOCUS       macroH2A1-1-F192V                 5834 bp    DNA     circular SYN 21-JUN-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     CDS             4433..5227
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2483..3202
                     /note="pLannotate"
                     /label="EGFP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(3480..3908)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        4041..4398
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        764..1067
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        1068..1271
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    3325..3446
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        3935..4039
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1322..2425
                     /note="pLannotate"
                     /label="Macroh2a1"
                     /database="swissprot"
                     /identity="93.0"
                     /match_length="99.2"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    5459..5506
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     misc_feature    2425..2469
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="84.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             5248..5400
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    1294..1321
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3219..3302
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     polyA_signal    complement(5597..5613)
                     /note="pLannotate"
                     /label="bGH poly(A) signal (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.2"
                     /fragment="True"
                     /other="polyA_signal"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
     1321 gatgtcgagc cgtggtggga agaagaaatc caccaagacc tcccggtcag ccaaggccgg
     1381 ggtcatcttc cccgtgggac ggatgctgcg gtacatcaag aaaggccacc ctaagtatcg
     1441 gatcggagtg ggggcgcctg tgtacatggc tgccgtactg gaatacctga ccgctgagat
     1501 cctggagctg gccggcaatg cagcgagaga caacaagaag gggcgggtca cgccgcggca
     1561 catcctgtta gcagtggcca atgatgagga gctaaaccag ctgctaaaag gagtcaccat
     1621 agccagtggg ggcgtgttgc cgaacatcca tcctgagttg ctagcgaaga aacgaggatc
     1681 caagggaaaa ttggaagcca tcatcacgcc tccaccagcc aaaaaggcca agtctccatc
     1741 ccagaagaag ccagtggcta agaaaacagg aggcaagaaa ggggcccgga agtctaagaa
     1801 gcagggagaa gtcagcaagg cagccagcgc agacagtaca acggagggcg cgcctacaga
     1861 cggctttact gtcctctcta ccaagagcct cgtgctcggc cagaagttgc aagttgttca
     1921 ggctgacatt gcctcgatcg acagtgatgc tgtcgttcac ccgacaaaca ctgacttcta
     1981 catcggtggt gaagtaggaa gcacactgga gaagaaaggt ggcaaggagt ttgtagaagc
     2041 tgttctggaa cttcggaaaa agaacgggcc cttggaagta gctggagctg ctgttagtgc
     2101 aggccacggc ctgcctgcca agtttgtgat ccactgtaac agtcctgtct ggggcgcaga
     2161 caaatgtgaa gaacttctag aaaagacggt gaaaaactgc ctggctctag ctgatgacag
     2221 aaagctgaag tccatcgcct tcccgtctat tggcagtggc aggaacgggt tcccgaagca
     2281 gacagccgcc caactcattc tgaaggccat ctccagctac tttgtgtcca ctatgtcgtc
     2341 ctccatcaaa actgtgtact ttgtgctttt tgacagtgag agcataggca tctacgtgca
     2401 ggaaatggcc aaactggatg ccaacaagct tcgaattctg cagtcgacgg taccgcgggc
     2461 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2521 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2581 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2641 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     2701 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2761 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2821 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2881 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     2941 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3001 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3061 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3121 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3181 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     3241 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3301 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3421 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     3481 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3541 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3601 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3661 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3721 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3781 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3841 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3901 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3961 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4021 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4081 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4141 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4201 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4261 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4321 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4381 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4441 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4501 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4561 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4621 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4681 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4741 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4801 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4861 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4921 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4981 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5041 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5101 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5161 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5221 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5281 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5341 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5401 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5461 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5521 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5581 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5641 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5701 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5821 atcaaaggat cttc
//
