LOCUS       Exported                6581 bp ds-DNA     circular SYN 19-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 1, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(2881..2890,3317..4171)
                     /organism="pPD95_62"
                     /mol_type="other DNA"
                     /label=source:pPD95_62
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    169..1831
                     /label=unc-25
     misc_feature    169..240
                     /label=epg-3
     misc_feature    1231..1831
                     /label=pMG168 (DD, VD, RME)
     misc_feature    1673..1690
                     /label=EV unc-25 End FWD
     misc_feature    1788..1831
                     /label=unc-25a
     misc_feature    1937..1978
                     /label=1xNLS
     misc_feature    1938..1957
                     /label=Color FWD
     misc_feature    1979..2014
                     /label=NLS2
     misc_feature    join(2015..2188,2240..2422,2474..2665,2717..2866)
                     /label=ceBFP2
     misc_feature    2348..2365
                     /label=EV ceBFP2 Mid FWD
     misc_feature    2396..2415
                     /label=EV ceBFP2 Mid REV
     misc_feature    2503..2521
                     /label=EV ceBFP2 Mid2 FWD
     misc_feature    2629..2646
                     /label=EV ceBFP2 Mid2 REV
     misc_feature    2636..2638
                     /label=I to A mTagBFP2 Fix
     misc_feature    complement(2867..2872)
                     /label=Linker AvrII Cut Site
     misc_feature    complement(2873..2874)
                     /label=Frame Link
     misc_feature    complement(2875..2880)
                     /label=AgeI Cut Site
     misc_feature    2891..3256
                     /label=H2B
     misc_feature    3038..3057
                     /label=H2B Mid REV
     misc_feature    3257..3262
                     /label=EcoRI Cut Site
     misc_feature    3263..3316
                     /label=Ligated Addition
     misc_feature    3385..4112
                     /label=unc-54 3'UTR
     misc_feature    3395..3413
                     /label=EV unc-54 3'UTR REV
     misc_feature    3863..4015
                     /label=aex-5 Exon1
     primer_bind     complement(4113..4138)
                     /label=Primer D54
     primer_bind     complement(4133..4158)
                     /label=PrimerD858
     misc_feature    4150..4167
                     /label=EV Landmark REV
     primer_bind     complement(4172..4194)
                     /label=3GEX_GW_PRIMER
     misc_feature    4172..4189
                     /label=EV ceBFP2 + H2B GIB REV
     CDS             4780..5146
                     /label=AmpR
     CDS             5147..5439
                     /label=AmpR
                     /label=AmpR(1)
     rep_origin      5537..6219
                     /label=ColE1 origin
     misc_binding    6559..6581
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc gcacgtcttc
      181 ttattcttct tcttcttctt cttttcctac cttttctatc ccactttttc cccggaattt
      241 tgagatttga catgattttt cagagaattt cgagttttga gaaaaaaaaa atcaaaaaag
      301 cgattttttg gtcaaaagcc gaaatttaaa gctagttttt ttggaaatat cgattttttt
      361 tttgagaatt tttcgatttt ccaaaaaaaa aatcgaaagt tgtttcccga aaagccgaaa
      421 ttttttttga gatatcgaaa aatcggagaa tttttcgatg tttcccagaa atactaaaat
      481 tttgaatttt ccggaaaaaa aatcgttaaa aaattagttt cttttaaaga ccgaaattta
      541 aagttttttt tttggaaata tcgatttttt ccgagatatc taaaattgaa agaaattttc
      601 gattttctga gaaaaatacg aaaaaatcga aaaaaaaacc caaatttcgg ttttttcgta
      661 aaaaaagtcg taaaaatgta atttttttcc tgaaaaatcg gaatttttta caaaatatcg
      721 gaaaatactc aaaaaaaagc tgaaaatttc gattttccgg agaaaaaatc tttttaaaaa
      781 aatatttttt ttttcagaaa ataagaaaag ccgaaattta taactatttc tccggaaatt
      841 cgaaattttt aacgaaatat cggaataatt tttagatttt tcaagtttga ctttgcgaga
      901 aaaaaatcgg aaaaacctcg attcgacgcc gaaaaatgct ccttttcgaa aagatttttg
      961 aaatttcaga aaatcgacat gcaagcgcgc tccacggcga aatgacaacg atgatccacc
     1021 gccctcaaaa agttgggtct cgttaggtat ttggcggtaa aactggtaaa actccagttt
     1081 tgcctccaac gagacccaat tttttggggc ggtggtggag cgcgcttgca caagctgaaa
     1141 gcatttttct gcgactcgat aatattttga aaacctgtgt caattctcga aattcttttt
     1201 ttaaaaaata atcccgagct tctctcagtc ctcctctatg aggatgttcc ttttttttgg
     1261 tttttcaatt ttttttaaaa ttccaaattt ctgttgtgca attcacttcc ccccaagaaa
     1321 tcccaaaaat ccccagtttt ccccaaaaat gttccgtttt catgtgattt ttcccccatt
     1381 tttaaaacat ttttttgact tttttttaaa atgattatta ttattgtttt ctatttcatg
     1441 gccggtaaat tatttttttt ctttcttttt ttttgctctt ttttttcaag aattttcgaa
     1501 ttgtttgaag ggctgctcat ctaatctttt gtcattttgt tctgatgcca tcatttctga
     1561 gaggaccttt gaagactcgt cacgaaacgg gaggggggct caagtgagca ttattattat
     1621 tattattgtc gcaaaaagtt taccccgggc tccccctggc tcccctcttt gagcaagggt
     1681 ttaagggctc attttgatga cgaattgctc attgggatta tagtcacgcc cctcttttgg
     1741 agcaactaca caactgagcc acagtaatcc ttgggggcgg ggtcagtagg accccctccg
     1801 gaatagggaa aagctcagtt caccgccaaa acccgggatt ggccaaagga cccaaaggta
     1861 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg cttggagggt
     1921 accgagctca gaaaaaatga ccgctccaaa gaagaagcgc aaggttccag tcgagaagat
     1981 gactgcccca aaaaagaaac gtaaggtccc gcggatgtca gagcttatta aggagaatat
     2041 gcatatgaaa ctgtacatgg aaggaaccgt ggacaaccat cactttaagt gcacttcgga
     2101 gggggaaggc aaaccgtacg agggcaccca gaccatgcga atcaaggtgg tcgagggagg
     2161 cccactgccc ttcgccttcg acattctggt aagtttaaac atatatatac taactaaccc
     2221 tgattattta aattttcagg ctactagctt cctgtacggc agcaagactt ttattaatca
     2281 cacccagggc atcccggact tcttcaaaca gtcgttccct gagggcttca catgggagcg
     2341 tgtgaccaca tacgaagacg gtggcgttct caccgctacc caggacacca gcctccagga
     2401 cggttgcctc atatataatg tggtaagttt aaacagttcg gtactaacta accatacata
     2461 tttaaatttt cagaagatcc ggggagtaaa cttcacatcc aatggtccgg ttatgcagaa
     2521 gaaaacactc ggctgggagg ccttcaccga gacgctgtat ccggctgacg gaggcctgga
     2581 aggtcgaaac gacatggccc tgaagctcgt cggcggtagc catctcatcg caaacgctaa
     2641 gaccacatat agatccaaga aaccggtaag tttaaacatg attttactaa ctaactaatc
     2701 tgatttaaat tttcaggcta agaaccttaa aatgccggga gtctactatg ttgactacag
     2761 actggaacgg atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggttgcagt
     2821 tgccagatac tgcgacctcc cgagcaaact gggtcacaag cttaatccta ggataccggt
     2881 gggtgaagac ccaccaaagc catctgccaa gggagccaag aaggccgcca agaccgttac
     2941 gaagccaaag gacggaaaga agagacgtca tgcccgtaag gaatcatact ccgtctacat
     3001 ctaccgtgtc ctcaagcaag ttcatccaga cactggagtt tcctccaaag ccatgtctat
     3061 catgaactct tttgtcaacg atgtcttcga gcgtattgct gctgaagcat cccgtcttgc
     3121 tcactacaac aagcgttcca caatctcatc ccgcgaaatt cagaccgctg tccgtctgat
     3181 ccttccagga gagcttgcca agcacgccgt gtctgaggga accaaggccg ttaccaagta
     3241 cacttccagc aagtaggaat tccaactgag cgccggtcgc taccattacc aacttgtctg
     3301 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     3361 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     3421 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     3481 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     3541 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     3601 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
     3661 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
     3721 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
     3781 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
     3841 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc
     3901 ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     3961 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     4021 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     4081 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     4141 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4201 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     4261 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     4321 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4381 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4441 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4561 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4621 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4681 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4741 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4801 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4861 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4921 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4981 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5041 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5101 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5161 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5221 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5281 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5341 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5401 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5461 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5521 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6001 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6361 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6481 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6541 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t
//
