LOCUS       Exported                5471 bp ds-DNA     circular SYN 01-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 1, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    169..1007
                     /label=hlh-34
     misc_feature    893..912
                     /label=EV hlh-34 End FWD
     misc_feature    1034..1075
                     /label=artificial intron
     misc_feature    1090..1095
                     /label=KpnI Cut Site
     primer_bind     1096..1113
                     /label=M13F-41_GW_PRIMER
     misc_feature    1112..1117
                     /label=MluI Cut Site
                     /label=MluI Cut Site(1)
     misc_feature    1118..1123
                     /label=KpnI Cut Site
                     /label=KpnI Cut Site(1)
     misc_feature    1137..1217
                     /label=2xNLS
     misc_feature    1256..1261
                     /label=MluI Cut Site
     misc_feature    1258..1278
                     /label=EV NLS3 intron REV
     misc_feature    1263..1265
                     /label=ORF ATG
     misc_feature    1269..1319
                     /label=intron
     misc_feature    1319..1324
                     /label=BssHII Cut Site
     CDS             join(1392..1415,1418..1454,1456,1458..1553,1581..1586,
                     1638..1812,1815..1952,2010..2159)
                     /codon_start=1
                     /label=mCardinal
                     /translation="MNHHFKCTRRGGQALRGHPDQGLRWWREAPCRSHSTSWPPALCTG
                     ARPSSTTPPSLRASHGRESPHTKTGACLPLPRTPASRTAA*STTSSSEG*TSHPTAL*C
                     RRKHSAGRPTETLYPADGGLEGRCDMALKLVGGGHLHCNLKTTYRSKKPAKNLKMVYFV
                     DRRLERIKEADNETYVEQHEVAVARYCDLPSKLGHKLNGMDELYK"
     misc_feature    1518..1520
                     /label=Same as mBeRFP Start
     misc_feature    1521..1538
                     /label=EV mNeptune2.5 Mid FWD
     misc_feature    1587..1637
                     /label=intron 2
     misc_feature    1632..1649
                     /label=EV mBeRFP/mNep2.5 intron3 Mid FWD
     misc_feature    1638..1644
                     /label=Bsu36I Cut Site
     misc_feature    1644..1661
                     /label=EV mNeptune2.5 Mid REV
     misc_feature    1659..1661
                     /label=Same as mBeRFP End
     misc_feature    1925..1944
                     /label=EV mCardinal Mid REV
     misc_feature    1946..1968
                     /label=EV mNeptune2.5 intron MID REV
     misc_feature    1959..2009
                     /label=intron 3
     misc_feature    2010..2015
                     /label=AccI Cut Site
     misc_feature    2133..2135
                     /label=Last mChOpti Difference
     misc_feature    2160..2982
                     /label=unc-54 3' UTR
     misc_feature    2174..2195
                     /label=EV unc-54 3'UTR GIB REV
     misc_feature    2285..2303
                     /label=EV unc-54 3'UTR REV
     misc_feature    3040..3057
                     /label=EV Landmark REV
     primer_bind     complement(3062..3084)
                     /label=3GEX_GW_PRIMER
     CDS             3670..4329
                     /label=AmpR
     rep_origin      4427..5109
                     /label=ColE1 origin
     misc_binding    5449..5471
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg aagttcatgg
      181 acagttgagt aagtgtattt tgactccaag gcaacgtatg cctgcaccag gagtgtttcg
      241 taaaatttac tgggctacag aaattgttgt atttttctaa tagactcaaa actataccaa
      301 aactgaattt cataatgaga cctcttgaaa atgtctttca aaaaatttca gttgtggttt
      361 aaagtttcga aaactgagaa aaaagaccca cactaatgct aaaataagaa aatcaaaaaa
      421 aaaaaaccga acttcacaaa aaactacgaa agttacgcag gtatgatacc tgcagatgtg
      481 cctgcctacc acataatcga tagttttaac tgcttatttt ttttaggatt atgtgaagcc
      541 gaaggatcat gtcgacattg tgcagacagt agatattcat actatcgatg ccgtatgcag
      601 gatgactgtt ttattggcga aatctgtgat aatggatttt gctgtcccaa cgttttgcca
      661 acattttctc ttcaacgaga acgtgagttt ctatcttgtt ttattttttg cctacaatta
      721 actatcaata cttttactaa cataatctaa cacactgcac tatttctcag caactagtcc
      781 taaaccaaaa agaccattaa atatcacatc ggatccccca gctgatgagc catgtccaga
      841 tgaatctaga tggtcaaaaa ggtgctcaag agatgaggat tgtactcatc aagaagaact
      901 ttgcatcgag ggaaaatgtt gtaaaagtgc gtttattttt taaatttgaa accaaagtgt
      961 ttatcttctt tgaaattagc gtaataaaaa aagaaataaa atgagaaccc gggattggcc
     1021 aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga
     1081 cccttggagg gtaccggttt tcccagtcac gacgcgtggt accgagctca gaaaaaatga
     1141 ccgctccaaa gaagaaacgc aaagtaccga gctcagaaaa aatgacggca cctaaaaaga
     1201 aacgcaaagt accggtcgcc gagaagacag ccccaaaaaa gaagcgtaaa gttccacgcg
     1261 tcatgggagt aagtttaaac agttcggtac taactaacca tacatattta aattttcagc
     1321 gcgctgtctc caagggagag gagctcatca aggagaacat gcacaccaag ctctacatgg
     1381 agggaaccgt caacaaccac cacttcaagt gcacccacga aggggagggc aagccctacg
     1441 agggcaccca gaccaacagg attaaggtgg tggagggagg ccccctgccg ttcgcattcg
     1501 acatcctggc cacctgcttt atgtacggga gcaagacctt catcaaccac acccaaggaa
     1561 tcccagactt cttcaagcaa tccttcgtaa gtttaaacat gattttacta actaactaat
     1621 ctgatttaaa ttttcagcct gagggcttca catgggagag agtcaccaca tacgaagacg
     1681 ggggcgtgct taccgttacc caggacacca gcctccagga cggctgcttg atctacaacg
     1741 tcaagctcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
     1801 gctgggaggc cagtaccgag accctgtacc ccgctgacgg cggcctggaa ggcagatgcg
     1861 acatggccct gaagctcgtg ggcgggggcc acctgcactg caacctgaag accacataca
     1921 gatccaagaa acccgctaag aacctcaaga tgccaggagt aagtttaaac atatatatac
     1981 taactaaccc tgattattta aattttcagg tctactttgt ggaccgcaga ctggaaagaa
     2041 tcaaggaggc cgacaatgag acctacgtcg agcagcacga ggtggctgtg gccagatact
     2101 gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtaag
     2161 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     2221 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     2281 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     2341 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     2401 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     2461 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     2521 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     2581 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     2641 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     2701 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     2761 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     2821 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     2881 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     2941 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     3001 ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     3061 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     3121 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     3181 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     3241 gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt
     3301 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3481 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3541 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3601 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3661 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     3721 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3781 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3841 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3901 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3961 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4021 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4081 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4141 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4201 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4261 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4321 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4381 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4441 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4501 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4561 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4621 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     4681 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4741 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4801 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4861 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     4921 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4981 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5041 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5101 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5161 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5221 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5281 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5341 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     5401 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     5461 ggataacaat t
//
