LOCUS       Exported                5666 bp ds-DNA     circular SYN 01-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 1, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    170..1202
                     /label=nsy-5 prom 3
     misc_feature    973..991
                     /label=EV nsy-5 1.2K FWD
     misc_feature    1229..1270
                     /label=artificial intron
     misc_feature    1285..1290
                     /label=KpnI Cut Site
     primer_bind     1291..1308
                     /label=M13F-41_GW_PRIMER
     misc_feature    1307..1312
                     /label=MluI Cut Site
                     /label=MluI Cut Site(1)
     misc_feature    1313..1318
                     /label=KpnI Cut Site
                     /label=KpnI Cut Site(1)
     misc_feature    1332..1412
                     /label=2xNLS
     misc_feature    1451..1456
                     /label=MluI Cut Site
     misc_feature    1453..1473
                     /label=EV NLS3 intron REV
     misc_feature    1458..1460
                     /label=ORF ATG
     misc_feature    1464..1514
                     /label=intron
     misc_feature    1514..1519
                     /label=BssHII Cut Site
     CDS             join(1587..1610,1613..1649,1651,1653..1748,1776..1781,
                     1833..2007,2010..2147,2205..2354)
                     /codon_start=1
                     /label=mCardinal
                     /translation="MNHHFKCTRRGGQALRGHPDQGLRWWREAPCRSHSTSWPPALCTG
                     ARPSSTTPPSLRASHGRESPHTKTGACLPLPRTPASRTAA*STTSSSEG*TSHPTAL*C
                     RRKHSAGRPTETLYPADGGLEGRCDMALKLVGGGHLHCNLKTTYRSKKPAKNLKMVYFV
                     DRRLERIKEADNETYVEQHEVAVARYCDLPSKLGHKLNGMDELYK"
     misc_feature    1713..1715
                     /label=Same as mBeRFP Start
     misc_feature    1716..1733
                     /label=EV mNeptune2.5 Mid FWD
     misc_feature    1782..1832
                     /label=intron 2
     misc_feature    1827..1844
                     /label=EV mBeRFP/mNep2.5 intron3 Mid FWD
     misc_feature    1833..1839
                     /label=Bsu36I Cut Site
     misc_feature    1839..1856
                     /label=EV mNeptune2.5 Mid REV
     misc_feature    1854..1856
                     /label=Same as mBeRFP End
     misc_feature    2120..2139
                     /label=EV mCardinal Mid REV
     misc_feature    2141..2163
                     /label=EV mNeptune2.5 intron MID REV
     misc_feature    2154..2204
                     /label=intron 3
     misc_feature    2205..2210
                     /label=AccI Cut Site
     misc_feature    2328..2330
                     /label=Last mChOpti Difference
     misc_feature    2355..3177
                     /label=unc-54 3' UTR
     misc_feature    2369..2390
                     /label=EV unc-54 3'UTR GIB REV
     misc_feature    2480..2498
                     /label=EV unc-54 3'UTR REV
     misc_feature    3235..3252
                     /label=EV Landmark REV
     primer_bind     complement(3257..3279)
                     /label=3GEX_GW_PRIMER
     CDS             3865..4524
                     /label=AmpR
     rep_origin      4622..5304
                     /label=ColE1 origin
     misc_binding    5644..5666
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga aagaaagaac
      181 attttaaaca tggggcatcg tggccaaaat aacaaagtgt gctcctaatt cgccatactt
      241 tacgcgcaaa gttaaaaaat tttattggaa aagaacatta atatgcttga tttaatttta
      301 cgctaaaatt ttgaattttt gaaaaaaaat ttcaaatttt aagttttcaa aaaaattttt
      361 tttttcaatt tcaaattttc aaaaaaaaat ttataccaaa atacctaccc aaaaaaagtt
      421 actttaaaaa tcaatatagc gcaataactt ctggaaattt gacgaatttt ttttcaaagc
      481 agtaatcaag aaacaaattc aaaaaaatca aatactaggc tcattcaaga ataaaaacaa
      541 atttaaattt ccaattttca gtagaaattt tttccaactt ttgtcttttt gcaaacgatg
      601 ccccacagtg agcttccacg tcaccactta tcccatttcc ttaccgccac tttgcgcctt
      661 ctaatccgtc catttcaaaa ctcgagtact tttctttctc ccggcctttc tccatgtcat
      721 cccacacctc ttcccgtgct aattgccacg gaaatagtgt agaagcctac gagacgcagc
      781 acacaacgtt ttgcttatta gtacgttcat tgaaatgagc agcagaagag atgaagtcgt
      841 ccttttgaca cataccctac tttagattat tattattatt actactacta ctactataga
      901 attcctatta tacatctggt aactccaata attcgacgaa cgtataataa gaaaattatt
      961 aagaaaagtg agtcgttccg atgacagatg ggaccttttt ttcaatatta ggaaaattat
     1021 ttttgaatca cctgagaatg tcgcatgccc acacagatac acaaacattt ttcaaaattt
     1081 ctgattttct aggttcgtgg gctagaaaac taaagaaaaa actcggccac caattttttc
     1141 cggtggccga gttttctgag tttctcggcc actaagtggg ggctaggctg tcccaccacg
     1201 ttcccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga
     1261 acattttcag gaggaccctt ggagggtacc ggttttccca gtcacgacgc gtggtaccga
     1321 gctcagaaaa aatgaccgct ccaaagaaga aacgcaaagt accgagctca gaaaaaatga
     1381 cggcacctaa aaagaaacgc aaagtaccgg tcgccgagaa gacagcccca aaaaagaagc
     1441 gtaaagttcc acgcgtcatg ggagtaagtt taaacagttc ggtactaact aaccatacat
     1501 atttaaattt tcagcgcgct gtctccaagg gagaggagct catcaaggag aacatgcaca
     1561 ccaagctcta catggaggga accgtcaaca accaccactt caagtgcacc cacgaagggg
     1621 agggcaagcc ctacgagggc acccagacca acaggattaa ggtggtggag ggaggccccc
     1681 tgccgttcgc attcgacatc ctggccacct gctttatgta cgggagcaag accttcatca
     1741 accacaccca aggaatccca gacttcttca agcaatcctt cgtaagttta aacatgattt
     1801 tactaactaa ctaatctgat ttaaattttc agcctgaggg cttcacatgg gagagagtca
     1861 ccacatacga agacgggggc gtgcttaccg ttacccagga caccagcctc caggacggct
     1921 gcttgatcta caacgtcaag ctcagagggg tgaacttccc atccaacggc cctgtgatgc
     1981 agaagaaaac actcggctgg gaggccagta ccgagaccct gtaccccgct gacggcggcc
     2041 tggaaggcag atgcgacatg gccctgaagc tcgtgggcgg gggccacctg cactgcaacc
     2101 tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgcca ggagtaagtt
     2161 taaacatata tatactaact aaccctgatt atttaaattt tcaggtctac tttgtggacc
     2221 gcagactgga aagaatcaag gaggccgaca atgagaccta cgtcgagcag cacgaggtgg
     2281 ctgtggccag atactgcgac ctccctagca aactggggca caaacttaat ggcatggacg
     2341 agctgtacaa gtaagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata
     2401 ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta
     2461 aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     2521 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     2581 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     2641 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     2701 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     2761 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     2821 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     2881 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     2941 aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca
     3001 agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     3061 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     3121 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     3181 accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg
     3241 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     3301 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     3361 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     3421 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc
     3481 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3541 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3601 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3661 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3721 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3781 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3841 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3901 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3961 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4021 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4081 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4141 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4201 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4261 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4321 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4381 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4441 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4501 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4561 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4621 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4681 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4741 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4801 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4861 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4921 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4981 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5041 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     5101 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5161 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5221 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5281 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5341 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5401 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5461 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5521 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5581 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5641 tgtggaattg tgagcggata acaatt
//
