LOCUS       Exported                5891 bp ds-DNA     circular SYN 29-OCT-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 1, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2627..3481
                     /organism="pPD95_62"
                     /mol_type="other DNA"
                     /label=source:pPD95_62
     primer_bind     6..22
                     /label=M13R_GW_PRIMER
     misc_feature    113..131
                     /label=EV Landmark FWD
     misc_feature    169..1135
                     /label=EV srh-15
     misc_feature    169..232
                     /label=srh-18
     misc_feature    169..232
                     /label=srh-18 exons
     misc_feature    752..770
                     /label=EV srh-15 End FWD
     misc_feature    1087..1107
                     /label=BC15959 REV
     misc_feature    1135
                     /label=srh-15 exons
     misc_feature    1135
                     /label=srh-15
     misc_feature    1241..1282
                     /label=1xNLS
     misc_feature    1242..1261
                     /label=Color FWD
     misc_feature    1283..1318
                     /label=NLS2
     misc_feature    1313..1318
                     /label=SacII Cut Site
     misc_feature    1369..1374
                     /label=BssHII Cut Site
     misc_feature    join(1376..1585,1637..1915,1967..2176)
                     /label=CyOFP1
     misc_feature    1411..1428
                     /label=EV CyOFP Start REV
     misc_feature    1684..1702
                     /label=EV CYOFP Mid2 FWD
     misc_feature    1751..1769
                     /label=EV CyOFP1 Mid FWD
     misc_feature    1803..1820
                     /label=EV CyOFP1 Mid REV
     misc_feature    1861..1879
                     /label=Ev CYOFP Mid3 FWD
     misc_feature    1991..2009
                     /label=EV CYOFP Mid3 REV
     misc_feature    1996..2014
                     /label=EV CyOFP Mid2 REV
     misc_feature    2177..2182
                     /label=AvrII Cut Site
     misc_feature    complement(2183..2184)
                     /label=Frame Link
     misc_feature    complement(2185..2190)
                     /label=AgeI Cut Site
     misc_feature    2201..2566
                     /label=H2B
     misc_feature    2348..2367
                     /label=H2B Mid REV
     misc_feature    2567..2572
                     /label=EcoRI Cut Site
     misc_feature    2573..2626
                     /label=Ligated Addition
     misc_feature    2695..3422
                     /label=unc-54 3'UTR
     misc_feature    2705..2723
                     /label=EV unc-54 3'UTR REV
     misc_feature    3173..3325
                     /label=aex-5 Exon1
     primer_bind     complement(3423..3448)
                     /label=Primer D54
     primer_bind     complement(3443..3468)
                     /label=PrimerD858
     misc_feature    3460..3477
                     /label=EV Landmark REV
     primer_bind     complement(3482..3504)
                     /label=3GEX_GW_PRIMER
     misc_feature    3482..3499
                     /label=EV ceBFP2 + H2B GIB REV
     CDS             4090..4456
                     /label=AmpR
     CDS             4457..4749
                     /label=AmpR
                     /label=AmpR(1)
     rep_origin      4847..5529
                     /label=ColE1 origin
     misc_binding    5869..5891
                     /label=LacO
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt tgagatgtca
      181 tgctttgaag aattcagtat caactgtaca atattcaagc caaaatactt aaaatgaact
      241 gcgtggattt tcgtaggtgt tacggtgaaa agcggacgta acttcataac cagtgaatat
      301 atcattaaat gaaaaaatga aaatgttttt gtggtgtttt cggtatttca ttaaccaaat
      361 aaattcactt tttgctttga tattttacat ttttgaaatt tcaacaagtt ggaatacaaa
      421 ctaccaaatt ttttccaaaa ggaaaaatat gaaatagacg gcttccttat ttatttgctt
      481 cgttgttggt taaatttgaa aaaaaaattt aaaaaatttc ttttgtacaa atttcgtcaa
      541 ttttttgtag gaaatttccg ctagaactga aattatttct gaaaaaataa tattttttaa
      601 tgagacagct caaaagattt tatttcagat aattatcttg aaaaatatat atacgtcttt
      661 ttgatcattg ttgagatttt agccagaatt accaatccac ctgttatgat cgttcatttt
      721 ttatttagta aacaagtcaa ctgtatagaa atcgcagtca ctcatgtcag tttttggttg
      781 ttttcaattg tcgaaaatta aaagctgaaa aaatcagaga gagtcgtctg aaaatgagct
      841 tgttccaata atttcgaaga gtataacatt gttaattgaa aacaattgtg tgtgattaga
      901 aatggtgttt tatttgaaat gtttttttga taaacacata attggtttac ataaaactac
      961 cttccccaaa aacgatgtga tcattggtac actgattgat tgatcacttt cttccaagaa
     1021 aatttaatta ccagaacatg aatgcaattt gattttgaag tggacgtcga tgaactccct
     1081 catataacat ctggtgctgt tgtcagatat ttgttttcac tcttgatttt atacacccgg
     1141 gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt
     1201 caggaggacc cttgcttgga gggtaccgag ctcagaaaaa atgaccgctc caaagaagaa
     1261 gcgcaaggtt ccagtcgaga agatgactgc cccaaaaaag aaacgtaagg tcccgcgggt
     1321 aagtttaaac agttcggtac taactaacca tacatattta aattttcagc gcgctgtgtc
     1381 aaaaggagaa gagcttatta aggagaatat gcgttccaag ctctacctcg agggatccgt
     1441 caacggacac caattcaagt gcacccacga gggagaggga aagccatacg agggaaagca
     1501 aaccaaccgt atcaaggtcg tcgagggagg accactccca ttcgccttcg acatcctcgc
     1561 cacccacttc atgtacggat ccaaggtaag tttaaacata tatatactaa ctaaccctga
     1621 ttatttaaat tttcaggtct tcatcaagta cccagccgac ctcccagact acttcaagca
     1681 atccttccca gagggattca cctgggagcg tgtcatggtc ttcgaggacg gaggagtcct
     1741 caccgccacc caagacacct ccctccaaga cggagagctc atctacaacg tcaaggtccg
     1801 tggagtcaac ttcccagcca acggaccagt catgcaaaag aagaccctcg gatgggagcc
     1861 atccaccgag accatgtacc cagccgacgg aggactcgag ggacgttgcg acaaggtaag
     1921 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcaggccc tcaagctcgt
     1981 cggaggagga cacctccacg tcaacttcaa gaccacctac aagtccaaga agccagtcaa
     2041 gatgccagga gtccactacg tcgaccgtcg tctcgagcgt atcaaggagg ccgacaacga
     2101 gacctacgtc gagcaatacg agcacgccgt cgcccgttac tccaacctcg gaggaggaat
     2161 ggacgagctc tacaagccta ggataccggt gggtgaagac ccaccaaagc catctgccaa
     2221 gggagccaag aaggccgcca agaccgttac gaagccaaag gacggaaaga agagacgtca
     2281 tgcccgtaag gaatcatact ccgtctacat ctaccgtgtc ctcaagcaag ttcatccaga
     2341 cactggagtt tcctccaaag ccatgtctat catgaactct tttgtcaacg atgtcttcga
     2401 gcgtattgct gctgaagcat cccgtcttgc tcactacaac aagcgttcca caatctcatc
     2461 ccgcgaaatt cagaccgctg tccgtctgat ccttccagga gagcttgcca agcacgccgt
     2521 gtctgaggga accaaggccg ttaccaagta cacttccagc aagtaggaat tccaactgag
     2581 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     2641 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     2701 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     2761 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     2821 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     2881 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     2941 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     3001 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     3061 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     3121 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     3181 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     3241 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     3301 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     3361 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     3421 ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     3481 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     3541 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     3601 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     3661 gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt
     3721 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3781 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3841 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3901 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3961 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4021 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4081 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4141 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4201 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4261 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4321 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4381 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4441 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4501 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4561 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4621 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4681 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4741 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4801 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4861 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4921 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4981 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5041 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     5101 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5161 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5221 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5281 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     5341 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5401 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5461 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5521 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5581 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5641 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5701 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5761 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     5821 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     5881 ggataacaat t
//
