LOCUS       CMV-pDisplay-Halo       6196 bp ds-DNA     circular     01-JUL-2024
DEFINITION  .
KEYWORDS    "marker:AmpR"
COMMENT     A new nucleotide sequence entered manually
FEATURES             Location/Qualifiers
     misc_feature    1..596
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created by: User modified by: User"
     misc_feature    638..657
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created by: User modified by: User"
     CDS             737..799
                     /label="Ig k-chain leader sequence"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="created by: User modified by: User"
     CDS             800..826
                     /label="HA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="created by: User"
     primer_bind     824..865
                     /label="MH0115"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="Sequence: GCTGGGGCCCAGCCGGCCAGATCAGGATCCGAAATCGGTACT created by: magnus"
     CDS             848..1738
                     /label="HaloTag7"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="Original Bases: ATGGACAAGGATTGTGAGATGAAGCGTACCACTCTGGATTCTCCACTGGGCAAGCTGGAGCTGTCTGGCTGTGAACAAGGTCTGCATGAGATTATCTTTCTGGGTAAGGGCACCTCCGCGGCGGATGCTGTTGAGGTTCCAGCGCCAGCGGCTGTGCTGGGCGGTCCAGAACCACTGATCCAAGCGACTGCGTGGCTGAATGCGTACTTCCATCAACCGGAAGCTATCGAGGAATTTCCGGTTCCAGCGCTGCACCATCCAGTTTTCCAACAAGAGAGCTTCACTCGCCAAGTTCTGTGGAAGCTGCTGAAGGTTGTTAAGTTTGGTGAAGTTATTAGCGAGAGCCACCTGGCTGCTCTGGTTGGTAATCCGGCTGCGACCGCTGCTGTTAACACTGCGCTGGACGGCAACCCAGTTCCGATCCTGATTCCATGTCATCGCGTTGTGCAAGGTGATTCCGATGTTGGCCCATATCTGGGTGGTCTGGCGGTTAAGGAGTGGCTGCTGGCTCATGAGGGCCATCGCCTGGGCAAGCCGGGTCTGGGTGGA modified by: User"
     misc_feature    848..1738
                     /label="Extracted region from pET51b_SNAP_CLIP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="Extracted interval: 5611 -> 6501 Original Circular Sequence Length: 6667"
     misc_feature    848..1738
                     /label="ACCTGGGACGAATGGCCAGAATTTGCCCGCGAGACCTTCCAGGCCTT..."
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="Original Bases: ACCTGGGACGAATGGCCAGAATTTGCCCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCAGAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGAGATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTTCCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATACATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGTTCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGTGGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGAGATCGCGCGCTGGCTGTCTACTCTGGAGATTGGTGGCACCGGTGGCTCTGGCGGTACCGGTGGCTCTGGCGGTTCTATCGGTACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGTCGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTCCTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGACCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGTCCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCACGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGGTATTGCATTTATGGAGTTCATCCGCCCTATC"
     primer_bind     complement(1722..1760)
                     /label="MH0114"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="Sequence: AGATGAGTTTTTGTTCGTCGACACCGGAAATCTCCAGAG created by: magnus modified by: magnus"
     CDS             1745..1774
                     /label="Myc epitop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="created by: User"
     CDS             1778..1927
                     /label="PDGFR transmembrane domain"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="created by: User"
     misc_feature    2040..2159
                     /label="BGH"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created by: User"
     misc_feature    2249..2922
                     /label="pUC origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created by: User"
     misc_feature    complement(3058..3329)
                     /label="TKpA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created by: User"
     misc_feature    complement(3237..4292)
                     /label="Kan/Neo"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created by: User modified by: User"
     misc_feature    complement(4327..4667)
                     /label="SV40 origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created by: User"
     misc_feature    complement(4747..5607)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="created by: User"
ORIGIN
        1 GCGCGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG
       61 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT
      121 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC
      181 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGACTATTT ACGGTAAACT GCCCACTTGG
      241 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT
      301 GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA
      361 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC
      421 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA
      481 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT
      541 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC
      601 TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA
      661 CCCAAGCTTG GTACCGAGCT CGGATCCACT AGTAACGGCC GCCAGTGTGC TGGAATTCGG
      721 CTTGGGGATA TCCACCATGG AGACAGACAC ACTCCTGCTA TGGGTACTGC TGCTCTGGGT
      781 TCCAGGTTCC ACTGGTGACT ATCCATATGA TGTTCCAGAT TATGCTGGGG CCCAGCCGGC
      841 CAGATCAGGA TCCGAAATCG GTACTGGCTT TCCATTCGAC CCCCATTATG TGGAAGTCCT
      901 GGGCGAGCGC ATGCACTACG TCGATGTTGG TCCGCGCGAT GGCACCCCTG TGCTGTTCCT
      961 GCACGGTAAC CCGACCTCCT CCTACGTGTG GCGCAACATC ATCCCGCATG TTGCACCGAC
     1021 CCATCGCTGC ATTGCTCCAG ACCTGATCGG TATGGGCAAA TCCGACAAAC CAGACCTGGG
     1081 TTATTTCTTC GACGACCACG TCCGCTTCAT GGATGCCTTC ATCGAAGCCC TGGGTCTGGA
     1141 AGAGGTCGTC CTGGTCATTC ACGACTGGGG CTCCGCTCTG GGTTTCCACT GGGCCAAGCG
     1201 CAATCCAGAG CGCGTCAAAG GTATTGCATT TATGGAGTTC ATCCGCCCTA TCCCGACCTG
     1261 GGACGAATGG CCAGAATTTG CCCGCGAGAC CTTCCAGGCC TTCCGCACCA CCGACGTCGG
     1321 CCGCAAGCTG ATCATCGATC AGAACGTTTT TATCGAGGGT ACGCTGCCGA TGGGTGTCGT
     1381 CCGCCCGCTG ACTGAAGTCG AGATGGACCA TTACCGCGAG CCGTTCCTGA ATCCTGTTGA
     1441 CCGCGAGCCA CTGTGGCGCT TCCCAAACGA GCTGCCAATC GCCGGTGAGC CAGCGAACAT
     1501 CGTCGCGCTG GTCGAAGAAT ACATGGACTG GCTGCACCAG TCCCCTGTCC CGAAGCTGCT
     1561 GTTCTGGGGC ACCCCAGGCG TTCTGATCCC ACCGGCCGAA GCCGCTCGCC TGGCCAAAAG
     1621 CCTGCCTAAC TGCAAGGCTG TGGACATCGG CCCGGGTCTG AATCTGCTGC AAGAAGACAA
     1681 CCCGGACCTG ATCGGCAGCG AGATCGCGCG CTGGCTGTCT ACTCTGGAGA TTTCCGGTGT
     1741 CGACGAACAA AAACTCATCT CAGAAGAGGA TCTGAATGCT GTGGGCCAGG ACACGCAGGA
     1801 GGTCATCGTG GTGCCACACT CCTTGCCCTT TAAGGTGGTG GTGATCTCAG CCATCCTGGC
     1861 CCTGGTGGTG CTCACCATCA TCTCCCTTAT CATCCTCATC ATGCTTTGGC AGAAGAAGCC
     1921 ACGTTAGGCG GCCGCTCGAG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT
     1981 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC
     2041 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
     2101 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT
     2161 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGTG GCGGTAATAC GGTTATCCAC
     2221 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     2281 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     2341 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     2401 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     2461 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     2521 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     2581 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     2641 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     2701 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
     2761 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     2821 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     2881 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     2941 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     3001 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC
     3061 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG
     3121 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG
     3181 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC
     3241 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT
     3301 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC
     3361 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC
     3421 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT
     3481 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG
     3541 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG
     3601 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG GTCCGCCACA
     3661 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC
     3721 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC
     3781 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT GATCATCCTG ATCGACAAGA
     3841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG
     3901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC
     3961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC
     4021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG
     4081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG
     4141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG
     4201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA
     4261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA
     4321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC
     4381 AGAGGCCGAG GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG
     4441 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC
     4501 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG
     4561 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC
     4621 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC
     4681 GCCTCAGGAC TCTTCCTTTT TCAATAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     4741 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     4801 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     4861 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
     4921 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     4981 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     5041 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     5101 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     5161 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     5221 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     5281 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     5341 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     5401 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     5461 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     5521 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     5581 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     5641 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     5701 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT
     5761 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC
     5821 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA
     5881 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
     5941 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT
     6001 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC
     6061 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC
     6121 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
     6181 AACGCTTACA ATTTAC
//