LOCUS       CMV-SNAP-hCB1_IRES_EGFP 7383 bp ds-DNA     circular     01-JUL-2024
DEFINITION  .
KEYWORDS    "marker:NeoR/KanR"
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus (CMV) immediate early promoter"
     misc_feature    624..683
                     /label="Seqsignal mGluR5"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
     misc_feature    684..734
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             735..1277
                     /label="SNAP-tag®"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="codon_start: 1 note: optimized for mammalian expression product: human O6-alkylguanine-DNA-alkyltransferase variant that forms covalent bonds with benzylguanine derivatives transl_table: 1 translation: DKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLAVKEWLLAHEGHRLGKPGLG"
     misc_feature    1284..2696
                     /label="CNR1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: human cannabinoid receptor 1 "
     misc_feature    2743..3317
                     /label="IRES2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     misc_feature    3318..3320
                     /label="IRES2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     misc_feature    3321..3329
                     /label="IRES2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     CDS             3330..4049
                     /label="AcGFP1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: Aequorea coerulescens GFP transl_table: 1 translation: MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFGFVTAAAITHGMDELYK*"
     polyA_signal    4171..4292
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: SV40 polyadenylation signal"
     rep_origin      complement(4299..4754)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        4781..4885
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     promoter        4887..5244
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 enhancer and early promoter"
     rep_origin      5095..5230
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 origin of replication"
     CDS             5279..6073
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    6305..6352
                     /label="HSV TK poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6681..7269
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
       61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
      121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
      181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
      241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
      301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
      361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
      421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
      481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
      541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA
      601 CCGGACTCAG ATCTCGAGCT CAAATGGTCC TTCTGTTGAT CCTGTCAGTC CTACTTCTGA
      661 AAGAAGATGT ACGAGGGAGT GCACAGTCCA CGCGATACCC CTACGACGTG CCCGACTACG
      721 CCACGCGTGG ATCCGACAAA GACTGCGAAA TGAAGCGCAC CACCCTGGAT AGCCCTCTGG
      781 GCAAGCTGGA ACTGTCTGGG TGCGAACAGG GCCTGCACGA GATCAAGCTG CTGGGCAAAG
      841 GAACATCTGC CGCCGACGCC GTGGAAGTGC CTGCCCCAGC CGCCGTGCTG GGCGGACCAG
      901 AGCCACTGAT GCAGGCCACC GCCTGGCTCA ACGCCTACTT TCACCAGCCT GAGGCCATCG
      961 AGGAGTTCCC TGTGCCAGCC CTGCACCACC CAGTGTTCCA GCAGGAGAGC TTTACCCGCC
     1021 AGGTGCTGTG GAAACTGCTG AAAGTGGTGA AGTTCGGAGA GGTCATCAGC TACCAGCAGC
     1081 TGGCCGCCCT GGCCGGCAAT CCCGCCGCCA CCGCCGCCGT GAAAACCGCC CTGAGCGGAA
     1141 ATCCCGTGCC CATTCTGATC CCCTGCCACC GGGTGGTGTC TAGCTCTGGC GCCGTGGGGG
     1201 GCTACGAGGG CGGGCTCGCC GTGAAAGAGT GGCTGCTGGC CCACGAGGGC CACAGACTGG
     1261 GCAAGCCTGG GCTGGGCACG CGTAAGTCGA TCCTAGATGG CCTTGCAGAT ACCACCTTCC
     1321 GCACCATCAC CACTGACCTC CTGTACGTGG GCTCAAATGA CATTCAGTAC GAAGACATCA
     1381 AAGGTGACAT GGCATCCAAA TTAGGGTACT TCCCACAGAA ATTCCCTTTA ACTTCCTTTA
     1441 GGGGAAGTCC CTTCCAAGAG AAGATGACTG CGGGAGACAA CCCCCAGCTA GTCCCAGCAG
     1501 ACCAGGTGAA CATTACAGAA TTTTACAACA AGTCTCTCTC GTCCTTCAAG GAGAATGAGG
     1561 AGAACATCCA GTGTGGGGAG AACTTCATGG ACATAGAGTG TTTCATGGTC CTGAACCCCA
     1621 GCCAGCAGCT GGCCATTGCA GTCCTGTCCC TCACGCTGGG CACCTTCACG GTCCTGGAGA
     1681 ACCTCCTGGT GCTGTGCGTC ATCCTCCACT CCCGCAGCCT CCGCTGCAGG CCTTCCTACC
     1741 ACTTCATCGG CAGCCTGGCG GTGGCAGACC TCCTGGGGAG TGTCATTTTT GTCTACAGCT
     1801 TCATTGACTT CCACGTGTTC CACCGCAAAG ATAGCCGCAA CGTGTTTCTG TTCAAACTGG
     1861 GTGGGGTCAC GGCCTCCTTC ACTGCCTCCG TGGGCAGCCT GTTCCTCACA GCCATCGACA
     1921 GGTACATATC CATTCACAGG CCCCTGGCCT ATAAGAGGAT TGTCACCAGG CCCAAGGCCG
     1981 TGGTGGCGTT TTGCCTGATG TGGACCATAG CCATTGTGAT CGCCGTGCTG CCTCTCCTGG
     2041 GCTGGAACTG CGAGAAACTG CAATCTGTTT GCTCAGACAT TTTCCCACAC ATTGATGAAA
     2101 CCTACCTGAT GTTCTGGATC GGGGTCACCA GCGTACTGCT TCTGTTCATC GTGTATGCGT
     2161 ACATGTATAT TCTCTGGAAG GCTCACAGCC ACGCCGTCCG CATGATTCAG CGTGGCACCC
     2221 AGAAGAGCAT CATCATCCAC ACGTCTGAGG ATGGGAAGGT ACAGGTGACC CGGCCAGACC
     2281 AAGCCCGCAT GGACATTAGG TTAGCCAAGA CCCTGGTCCT GATCCTGGTG GTGTTGATCA
     2341 TCTGCTGGGG CCCTCTGCTT GCAATCATGG TGTATGATGT CTTTGGGAAG ATGAACAAGC
     2401 TCATTAAGAC GGTGTTTGCA TTCTGCAGTA TGCTCTGCCT GCTGAACTCC ACCGTGAACC
     2461 CCATCATCTA TGCTCTGAGG AGTAAGGACC TGCGACACGC TTTCCGGAGC ATGTTTCCCT
     2521 CTTGTGAAGG CACTGCGCAG CCTCTGGATA ACAGCATGGG GGACTCGGAC TGCCTGCACA
     2581 AACACGCAAA CAATGCAGCC AGTGTTCACA GGGCCGCAGA AAGCTGCATC AAGAGCACGG
     2641 TCAAGATTGC CAAGGTAACC ATGTCTGTGT CCACAGACAC GTCTGCCGAG GCTCTGTAAG
     2701 CTTCGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CGCCCCTCTC CCTCCCCCCC
     2761 CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT
     2821 ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT
     2881 CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA
     2941 TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA CAAACAACGT CTGTAGCGAC
     3001 CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC CTCTGCGGCC AAAAGCCACG
     3061 TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC CACGTTGTGA GTTGGATAGT
     3121 TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC AAGGGGCTGA AGGATGCCCA
     3181 GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG TACACATGCT TTACATGTGT
     3241 TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG
     3301 AAAAACACGA TGATAATATG GCCACAACCA TGGTGAGCAA GGGCGCCGAG CTGTTCACCG
     3361 GCATCGTGCC CATCCTGATC GAGCTGAATG GCGATGTGAA TGGCCACAAG TTCAGCGTGA
     3421 GCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA
     3481 CCGGCAAGCT GCCTGTGCCC TGGCCCACCC TGGTGACCAC CCTGAGCTAC GGCGTGCAGT
     3541 GCTTCTCACG CTACCCCGAT CACATGAAGC AGCACGACTT CTTCAAGAGC GCCATGCCTG
     3601 AGGGCTACAT CCAGGAGCGC ACCATCTTCT TCGAGGATGA CGGCAACTAC AAGTCGCGCG
     3661 CCGAGGTGAA GTTCGAGGGC GATACCCTGG TGAATCGCAT CGAGCTGACC GGCACCGATT
     3721 TCAAGGAGGA TGGCAACATC CTGGGCAATA AGATGGAGTA CAACTACAAC GCCCACAATG
     3781 TGTACATCAT GACCGACAAG GCCAAGAATG GCATCAAGGT GAACTTCAAG ATCCGCCACA
     3841 ACATCGAGGA TGGCAGCGTG CAGCTGGCCG ACCACTACCA GCAGAATACC CCCATCGGCG
     3901 ATGGCCCTGT GCTGCTGCCC GATAACCACT ACCTGTCCAC CCAGAGCGCC CTGTCCAAGG
     3961 ACCCCAACGA GAAGCGCGAT CACATGATCT ACTTCGGCTT CGTGACCGCC GCCGCCATCA
     4021 CCCACGGCAT GGATGAGCTG TACAAGTGAG CGGCCGCGAC TCTAGATCAT AATCAGCCAT
     4081 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG
     4141 AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC
     4201 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT
     4261 TGTGGTTTGT CCAAACTCAT CAATGTATCT TAAGGCGTAA ATTGTAAGCG TTAATATTTT
     4321 GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT
     4381 CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT
     4441 TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT
     4501 CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG
     4561 GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG
     4621 AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC
     4681 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC
     4741 GCTACAGGGC GCGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT
     4801 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
     4861 TCAATAATAT TGAAAAAGGA AGAGTCCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT
     4921 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT
     4981 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG
     5041 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG
     5101 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT
     5161 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT
     5221 TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT
     5281 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG
     5341 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC
     5401 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA
     5461 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT
     5521 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA
     5581 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG
     5641 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT
     5701 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA
     5761 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG
     5821 CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG
     5881 CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT
     5941 AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT
     6001 CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA
     6061 CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG
     6121 CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT
     6181 TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC
     6241 CACCCTAGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT
     6301 ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGTGTTG GGTCGTTTGT TCATAAACGC
     6361 GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA
     6421 TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG
     6481 CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCCT CAGGTTACTC ATATATACTT
     6541 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT
     6601 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA
     6661 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA
     6721 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT
     6781 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG
     6841 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA
     6901 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
     6961 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG
     7021 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA
     7081 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA
     7141 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC
     7201 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC
     7261 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT
     7321 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCATG
     7381 CAT
//