LOCUS       CMV-SNAP-hCB2_IRES_EGFP 7047 bp ds-DNA     circular     01-JUL-2024
DEFINITION  .
KEYWORDS    "marker:NeoR/KanR"
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus (CMV) immediate early promoter"
     misc_feature    467..484
                     /label="CMV Forward "
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_feature    624..683
                     /label="Seqsignal mGlur5"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
                     /note="note: Rattus norvegicus glutamate metabotropic receptor 5 "
     misc_feature    684..734
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             735..1277
                     /label="SNAP-tag®"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="codon_start: 1 note: optimized for mammalian expression product: human O6-alkylguanine-DNA-alkyltransferase variant that forms covalent bonds with benzylguanine derivatives transl_table: 1 translation: DKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLAVKEWLLAHEGHRLGKPGLG"
     misc_feature    1028..1047
                     /label="5p SNAP forward"
                     /ApEinfo_revcolor="#000080"
                     /ApEinfo_fwdcolor="#000080"
     misc_feature    1284..2360
                     /label="CNR2 "
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: human cannabinoid receptor 2"
     misc_feature    1298..1315
                     /label="5p SNAP-CNR2 "
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    1912..1933
                     /label="5p CNR2 forward"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2407..2981
                     /label="IRES2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     misc_feature    2982..2984
                     /label="IRES2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     misc_feature    2985..2993
                     /label="IRES2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     CDS             2994..3713
                     /label="AcGFP1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: Aequorea coerulescens GFP transl_table: 1 translation: MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFGFVTAAAITHGMDELYK*"
     polyA_signal    3835..3956
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: SV40 polyadenylation signal"
     rep_origin      complement(3963..4418)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        4445..4549
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     promoter        4551..4908
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 enhancer and early promoter"
     rep_origin      4759..4894
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 origin of replication"
     CDS             4943..5737
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5969..6016
                     /label="HSV TK poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6345..6933
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
       61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
      121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
      181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
      241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
      301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
      361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
      421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
      481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
      541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA
      601 CCGGACTCAG ATCTCGAGCT CAAATGGTCC TTCTGTTGAT CCTGTCAGTC CTACTTCTGA
      661 AAGAAGATGT ACGAGGGAGT GCACAGTCCA CGCGATACCC CTACGACGTG CCCGACTACG
      721 CCACGCGTGG ATCCGACAAA GACTGCGAAA TGAAGCGCAC CACCCTGGAT AGCCCTCTGG
      781 GCAAGCTGGA ACTGTCTGGG TGCGAACAGG GCCTGCACGA GATCAAGCTG CTGGGCAAAG
      841 GAACATCTGC CGCCGACGCC GTGGAAGTGC CTGCCCCAGC CGCCGTGCTG GGCGGACCAG
      901 AGCCACTGAT GCAGGCCACC GCCTGGCTCA ACGCCTACTT TCACCAGCCT GAGGCCATCG
      961 AGGAGTTCCC TGTGCCAGCC CTGCACCACC CAGTGTTCCA GCAGGAGAGC TTTACCCGCC
     1021 AGGTGCTGTG GAAACTGCTG AAAGTGGTGA AGTTCGGAGA GGTCATCAGC TACCAGCAGC
     1081 TGGCCGCCCT GGCCGGCAAT CCCGCCGCCA CCGCCGCCGT GAAAACCGCC CTGAGCGGAA
     1141 ATCCCGTGCC CATTCTGATC CCCTGCCACC GGGTGGTGTC TAGCTCTGGC GCCGTGGGGG
     1201 GCTACGAGGG CGGGCTCGCC GTGAAAGAGT GGCTGCTGGC CCACGAGGGC CACAGACTGG
     1261 GCAAGCCTGG GCTGGGCACG CGTGAGGAAT GCTGGGTGAC AGAGATAGCC AATGGCTCCA
     1321 AGGATGGCTT GGATTCCAAC CCTATGAAGG ATTACATGAT CCTGAGTGGT CCCCAGAAGA
     1381 CAGCTGTTGC TGTGTTGTGC ACTCTTCTGG GCCTGCTAAG TGCCCTGGAG AACGTGGCTG
     1441 TGCTCTATCT GATCCTGTCC TCCCACCAAC TCCGCCGGAA GCCCTCATAC CTGTTCATTG
     1501 GCAGCTTGGC TGGGGCTGAC TTCCTGGCCA GTGTGGTCTT TGCATGCAGC TTTGTGAATT
     1561 TCCATGTTTT CCATGGTGTG GATTCCAAGG CTGTCTTCCT GCTGAAGATT GGCAGCGTGA
     1621 CTATGACCTT CACAGCCTCT GTGGGTAGCC TCCTGCTGAC CGCCATTGAC CGATACCTCT
     1681 GCCTGCGCTA TCCACCTTCC TACAAAGCTC TGCTCACCCG TGGAAGGGCA CTGGTGACCC
     1741 TGGGCATCAT GTGGGTCCTC TCAGCACTAG TCTCCTACCT GCCCCTCATG GGATGGACTT
     1801 GCTGTCCCAG GCCCTGCTCT GAGCTTTTCC CACTGATCCC CAATGACTAC CTGCTGAGCT
     1861 GGCTCCTGTT CATCGCCTTC CTCTTTTCCG GAATCATCTA CACCTATGGG CATGTTCTCT
     1921 GGAAGGCCCA TCAGCATGTG GCCAGCTTGT CTGGCCACCA GGACAGGCAG GTGCCAGGAA
     1981 TGGCCCGAAT GAGGCTGGAT GTGAGGTTGG CCAAGACCCT AGGGCTAGTG TTGGCTGTGC
     2041 TCCTCATCTG TTGGTTCCCA GTGCTGGCCC TCATGGCCCA CAGCCTGGCC ACTACGCTCA
     2101 GTGACCAGGT CAAGAAGGCC TTTGCTTTCT GCTCCATGCT GTGCCTCATC AACTCCATGG
     2161 TCAACCCTGT CATCTATGCT CTACGGAGTG GAGAGATCCG CTCCTCTGCC CATCACTGCC
     2221 TGGCTCACTG GAAGAAGTGT GTGAGGGGCC TTGGGTCAGA GGCAAAAGAA GAAGCCCCGA
     2281 GATCCTCAGT CACCGAGACA GAGGCTGATG GGAAAATCAC TCCGTGGCCA GATTCCAGAG
     2341 ATCTAGACCT CTCTGATTGC TAAGCTTCGA ATTCTGCAGT CGACGGTACC GCGGGCCCGG
     2401 GATCCGCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG
     2461 CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG
     2521 GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CCCCTCTCGC
     2581 CAAAGGAATG CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG AAGCTTCTTG
     2641 AAGACAAACA ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC CTGGCGACAG
     2701 GTGCCTCTGC GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG CACAACCCCA
     2761 GTGCCACGTT GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT CAAGCGTATT
     2821 CAACAAGGGG CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG ATCTGGGGCC
     2881 TCGGTACACA TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AAACGTCTAG GCCCCCCGAA
     2941 CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTGA
     3001 GCAAGGGCGC CGAGCTGTTC ACCGGCATCG TGCCCATCCT GATCGAGCTG AATGGCGATG
     3061 TGAATGGCCA CAAGTTCAGC GTGAGCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
     3121 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCTGT GCCCTGGCCC ACCCTGGTGA
     3181 CCACCCTGAG CTACGGCGTG CAGTGCTTCT CACGCTACCC CGATCACATG AAGCAGCACG
     3241 ACTTCTTCAA GAGCGCCATG CCTGAGGGCT ACATCCAGGA GCGCACCATC TTCTTCGAGG
     3301 ATGACGGCAA CTACAAGTCG CGCGCCGAGG TGAAGTTCGA GGGCGATACC CTGGTGAATC
     3361 GCATCGAGCT GACCGGCACC GATTTCAAGG AGGATGGCAA CATCCTGGGC AATAAGATGG
     3421 AGTACAACTA CAACGCCCAC AATGTGTACA TCATGACCGA CAAGGCCAAG AATGGCATCA
     3481 AGGTGAACTT CAAGATCCGC CACAACATCG AGGATGGCAG CGTGCAGCTG GCCGACCACT
     3541 ACCAGCAGAA TACCCCCATC GGCGATGGCC CTGTGCTGCT GCCCGATAAC CACTACCTGT
     3601 CCACCCAGAG CGCCCTGTCC AAGGACCCCA ACGAGAAGCG CGATCACATG ATCTACTTCG
     3661 GCTTCGTGAC CGCCGCCGCC ATCACCCACG GCATGGATGA GCTGTACAAG TGAGCGGCCG
     3721 CGACTCTAGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC
     3781 CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG
     3841 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
     3901 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAAGGC
     3961 GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
     4021 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
     4081 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
     4141 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
     4201 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
     4261 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
     4321 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
     4381 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA
     4441 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
     4501 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA
     4561 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
     4621 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
     4681 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
     4741 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
     4801 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA
     4861 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA
     4921 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC
     4981 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA
     5041 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT
     5101 GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC
     5161 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT
     5221 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT
     5281 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT
     5341 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT
     5401 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG
     5461 GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT
     5521 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG
     5581 TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG
     5641 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG
     5701 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG
     5761 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT
     5821 GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG
     5881 GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG
     5941 ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT
     6001 GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC
     6061 CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC
     6121 CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA
     6181 GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG
     6241 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
     6301 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT
     6361 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
     6421 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
     6481 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
     6541 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
     6601 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
     6661 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
     6721 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
     6781 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC
     6841 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
     6901 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG
     6961 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
     7021 GTGGATAACC GTATTACCGC CATGCAT
//