LOCUS       pUC-hTph2p(1915)-Luc_in 6655 bp ds-DNA     linear       22-APR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     GGAAaT          453..458
                     /label="Pet-1/Fev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     GGAAaT          complement(580..585)
                     /label="Pet-1/Fev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CACCTG          597..602
                     /label="Ebox"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CAGGTG          complement(597..602)
                     /label="Ebox"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CATGTG          complement(707..712)
                     /label="CRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CAGCTG          977..982
                     /label="Ebox/DEC1/2-MyoD1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CAGCTG          complement(977..982)
                     /label="Ebox/DEC1/2-MyoD1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1093..3007
                     /label="hTph2p(1915bp)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1093..3079
                     /label="hTph2p(1987bp)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     GGAAaT          complement(1388..1393)
                     /label="Pet-1/Fev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1539..1544
                     /label="RRE(ACTGGA)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(2000..2005)
                     /label="RRE"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     GGAAaT          complement(2019..2024)
                     /label="Pet-1/Fev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     GGAAaT          complement(2028..2033)
                     /label="Pet-1/Fev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CACATGT         complement(2155..2161)
                     /label="Ebox"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CATGTG          2156..2161
                     /label="CRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     GAGGAA          complement(2199..2204)
                     /label="SP1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CAGAGT          complement(2211..2216)
                     /label="Ebox"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CATGTG          complement(2556..2561)
                     /label="CRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CACATGT         2556..2562
                     /label="Ebox"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CACATGT         complement(2557..2563)
                     /label="Ebox"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CATGTG          2558..2563
                     /label="CRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     TAACGTCA        2764..2771
                     /label="CRE"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     TTCAGCACCAGG... 3015..3041
                     /label="NRSE"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CAGCTG          3051..3056
                     /label="Ebox/DEC1/2-MyoD1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CAGCTG          complement(3051..3056)
                     /label="Ebox/DEC1/2-MyoD1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     GAGGAA          complement(3069..3074)
                     /label="SP1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3136..4788
                     /label="luciferase"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3136..4788
                     /label="Translation 3136-4788"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     primer          complement(3137..3159)
                     /label="GL2"
                     /note="sequence: CTTTATGTTTTTGGCGTCTTCCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3609..3628
                     /label="Firefly Luc_qF"
                     /note="sequence: CCAGGGATTTCAGTCGATGT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(3772..3791)
                     /label="Firefly Luc_qR"
                     /note="sequence: AATCTCACGCAGGCAGTTCT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          4251..4270
                     /label="fluc-up"
                     /note="sequence: ggttgtggatctggataccg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4337..4356)
                     /label="fluc-dn"
                     /note="sequence: cgcttccggattgtttacat"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     polyA_signal    4829..4950
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CAGCTG          5268..5273
                     /label="Ebox/DEC1/2-MyoD1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CAGCTG          complement(5268..5273)
                     /label="Ebox/DEC1/2-MyoD1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     GAGGAA          complement(5333..5338)
                     /label="SP1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CACATGT         complement(5446..5452)
                     /label="Ebox"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CATGTG          5447..5452
                     /label="CRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CAGAGT          5905..5910
                     /label="Ebox"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CACGTT          6148..6153
                     /label="Ebox (noncanonical)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
       61 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
      121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
      181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
      241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
      301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
      361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
      421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
      481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
      541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
      601 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
      661 gcccttttgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      721 ggagactgtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      781 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
      841 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
      901 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      961 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     1021 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgacgcgtat
     1081 tgggatGGTA CCATTTGAGC CTTTAGGGTA TTCTGTCATG TAAAGTGCGT TGCTTCTAAG
     1141 TCTTCTTCAT TGATAGCTTA AGGTTCAGCT TTCCCATGAC TGCAAAGCCT TTGATCAGTT
     1201 TTTTCTTCTT TCAAGCTGCT AATTTTTTGT TGCCATTGTT TTCCAACTTA TGCGACTCTT
     1261 GTGTACCTTG AAAAGGAATT TTTTATCTTG TTTTAATAGG GTTTCCAGGG TGAGCAAAAG
     1321 TAGTTAAATC TTTAATATTC TAAATATATA TTATAAATAA ATTACTTTTA ATATCTTTTT
     1381 TTCCCTCATT TCCACATCCA AGCAGGGACA CTATCTAGTG GATTTTATCC CTTCACATCT
     1441 CTGGTTCTTT CATTACATCT CAGTAACATT AGCTACTATT ATTGTCATTA GTTCATTTGC
     1501 CTTATTTAAA CTTGCTTCTC CTATTAGCTG ATTAGAGCTG ACCCTTACAG AAAATGTAAG
     1561 CCAAATAATA TTTTCAGAAT TAGATCAATT AATAATTATG TCTCTTCAAG ACTCCAGAAA
     1621 CAATTAAACT TCAGTCAATA TTAGTTCATC CCGACTTGTT TTGAAATTTT TTTTCTATAT
     1681 AGACAAGCAG TGCCAGCACC AGATTTTGTT TAGATTTTCA GTCAAACAAG CACATTTGGT
     1741 CAAGAGTTTT TCATTCTTCA GGAGATTGGT AACATTTTCC TGTGGCCTGG TGAATTTTAT
     1801 GACTTTCTAA GGTTTGAATA GTAAGAATAT TGATTAATCT ATGCATGTCA AGTTGCTGGA
     1861 TTTGATCTAG TTGACATTGA TATTTGACAG TATGTTTAGT CATTAAAAGC TCAAATGTCA
     1921 TAGTACTCTT AACCTCTGCT TTCTCCTCAC CACATAACGC ACAGATCTCG TATATTTAAG
     1981 TAGCACTTTT TATATGAAAG GGTCATGTCA AAGATTAAAT TTCCATGATT TCCAGTAGAG
     2041 AGAAAAACCA CAAGAGTATT AAAATGAATG GCTGATATAT GGGAATCTAG TTTCCTTTTA
     2101 TTTCTCATTT AGTAAGGAAG ATTCCAGGAT TAGTATCTGA GCTATATGTA ATCTACATGT
     2161 GGCTAAATTG AACCCTTACC TTTCCTTTGC AATACATTTT CCTCCATATA ACTCTGCATA
     2221 GAGGCATCAC AGGATTAAGA AGAAGCCCTT TTATGAAAGC CATTACACAT ATATACACTC
     2281 ACACATTTGC ATGCACAAAA TTAGAATATG TCAAGTCAGA AAAAGCTTAT TAACATAAAA
     2341 TGGAGTTGGT CAATGAGTAA AAAAAATATG CTGATGGGAG GGATAAGATC TAGTGTTCGG
     2401 GAGCACAATA ATTTATTTTC TTTTGTATTT TAAAATAACT GGAAGAGTGG AATTGGAATG
     2461 TTTCTAACAC AAAAAGAAAT GATAAATGCT TGAGGCAATG GATATCTTGA TTACCTTATT
     2521 TGATCATTAC ACATTGTACG CTTGTGTCAA AATATCACAT GTGCCTTATA AATGTGTACA
     2581 ACTATTAGTT ATCCATAAAA ATTAAAAATT AAAAAATCCG TAAAATGGTT TAAGCATTCA
     2641 GCAGTGCTGA TCTTTCTTAA ATTATTTTTC TAATTTTGGA AAGAAAGCAC AAAATCTTTG
     2701 AATTCACAAT TGCTTAAAGA CTGAGGTTAA CTTGCCAGTG GCAGGCTTGA GAGATGAGAG
     2761 AACTAACGTC AGAGGATAGA TGGTTTCTTG TACAAATAAC ACCCCCTTAT GTATTGTTCT
     2821 CCACCACCCC CGCCCAAAAA GCTACTCGAC CTATGAAACA AATCACACTA TGAGCACAGA
     2881 TAACCCCAGG CTTCAGGTCT GTAATCTGAC TGTGGCCATC GGCAACCAGA AATGAGTTTC
     2941 TTTCTAATCA GTCTTGCATC AGTCTCCAGT CATTCATATA AAGGAGCCCG GGGATGGGAG
     3001 GATTCGCATT GCTCTTCAGC ACCAGGGTTC TGGACAGCGC CCCAAGCAGG CAGCTGATCG
     3061 CACGCCCCTT CCTCTCAATC TCGAGatctg cgatctaagt aagcttggca ttccggtact
     3121 gttggtaaag ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     3181 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     3241 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
     3301 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     3361 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     3421 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     3481 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
     3541 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
     3601 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     3661 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     3721 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     3781 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     3841 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     3901 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
     3961 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     4021 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     4081 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     4141 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     4201 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     4261 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     4321 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     4381 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     4441 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     4501 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
     4561 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     4621 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     4681 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     4741 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg
     4801 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     4861 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4921 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4981 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     5041 aaaatcgata aggatccgtc gaccgTCGAG ATCCCAATGG CGCGCCgagc ttggcgtaat
     5101 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5161 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5221 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5281 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctgttcc gcttcctcgc
     5341 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5401 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5461 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5521 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5581 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5641 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5701 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5761 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5821 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5881 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5941 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6001 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6061 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6121 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6181 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6241 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6301 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6361 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac
     6421 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6481 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6541 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6601 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagt
//