LOCUS       Exported                8015 bp ds-DNA     linear   UNA 06-APR-2017
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 5, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    4377..4382
                     /locus_tag="PstI"
                     /label=PstI
     misc_feature    4383..4475
                     /locus_tag="Signal Peptide"
                     /label=Signal Peptide
     misc_feature    4476..4559
                     /locus_tag="twinstrep(codon optimized)"
                     /label=twinstrep(codon optimized)
     misc_feature    4560..4583
                     /locus_tag="preSci recognition site"
                     /label=preSci recognition site
     misc_feature    4584..5129
                     /locus_tag="SNAP tag"
                     /label=SNAP tag
     misc_feature    5130..5147
                     /locus_tag="(GS)3"
                     /label=(GS)3
     misc_feature    5148..5168
                     /locus_tag="TVMV site"
                     /label=TVMV site
     misc_feature    5169..5171
                     /locus_tag="EcoRI"
                     /label=EcoRI
     CDS             5172..5771
                     /locus_tag="PDL2 ED (20-220aa)"
                     /label=PDL2 ED (20-220aa)
                     /note="/vntifkey=4"
     misc_feature    5772..5774
                     /locus_tag="EcoRI(1)"
                     /label=EcoRI
     misc_feature    5775..5804
                     /locus_tag="His10"
                     /label=His10
     misc_feature    5805..5807
                     /locus_tag="STOP"
                     /label=STOP
     misc_feature    5808..5815
                     /locus_tag="NotI"
                     /label=NotI
ORIGIN
        1 aaagacagca ttttcaacaa atggtgctgg cacaactggt ggttatcatg gagaagaatg
       61 tgaattgatc cattccaatc tccttgtact aaggtcaaat ctaagtggat caaggaactc
      121 cacataaaac cagagacact gaaacttata gaggagaaag tggggaaaag cctcgaagat
      181 atgggcacag gggaaaaatt cctgaataga acagcaatgg cttgtgctgt aagatcgaga
      241 attgacaaat gggacctcat gaaactccaa agctatcgga tcaattcctc caaaaaagcc
      301 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
      361 aaaaattagt cagccatgca tggggcggag aatgggcgga actgggcgga gttaggggcg
      421 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
      481 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
      541 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
      601 acacattcca cagaattaat tcccgatccg tcgacctcga gagcttggcg taatcatggt
      661 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      721 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      781 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      841 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgata tccgcttcct cgctcactga
      901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1621 gattacgcgc agaaaaaaag gatctcaaga agataccttt gatatttata cggggtctga
     1681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1861 tctaaacgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1921 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1981 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2041 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2101 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2161 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2221 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2281 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2341 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2401 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2461 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2521 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2581 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2641 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2701 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2761 aataaacaaa taggggttcc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     2821 atcgggagat ccgcgttgac attgattatt gactagttat taatagtaat caattacggg
     2881 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2941 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     3001 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     3061 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     3121 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     3181 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     3241 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3301 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     3361 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3421 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     3481 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
     3541 ccgtgccaag agtgacgtaa gtaccgccta tagactctat aggcacaccc ctttggctct
     3601 tatgcatgct atactgtttt tggcttgggg cctatacacc cccgcttcct tatgctatag
     3661 gtgatggtat agcttagcct ataggtgtgg gttattgacc attattgacc actcccctat
     3721 tggtgacgat actttccatt actaatccat aacatggctc tttgccacaa ctatctctat
     3781 tggctatatg ccaatactct gtccttcaga gactgacacg gactctgtat ttttacagga
     3841 tggggtccca tttattattt acaaattcac atatacaaca acgccgtccc ccgtgcccgc
     3901 agtttttatt aaacatagcg tgggatctcc acgcgaatct cgggtacgtg ttccggacat
     3961 gggctcttct ccggtagcgg cggagcttcc acatccgagc cctggtccca tgcctccagc
     4021 ggctcatggt cgctcggcag ctccttgctc ctaacagtgg aggccagact taggcacagc
     4081 acaatgccca ccaccaccag tgtgccgcac aaggccgtgg cggtagggta tgtgtctgaa
     4141 aatgagctcg gagattgggc tcgcaccgct gacgcagatg gaagacttaa ggcagcggca
     4201 gaagaagatg caggcagctg agttgttgta ttctgataag agtcagaggt aactcccgtt
     4261 gcggtgctgt taacggtgga gggcagtgta gtctgagcag tactcgttgc tgccgcgcgc
     4321 gccaccagac ataatagctg acagactaac agactgttcc tttccatggg tcttttctgc
     4381 agatggacgc tatgaagaga ggactgtgct gcgtgctgct gctgtgcgga gccgtgttcg
     4441 tgtcaccaag ccaggaaatc catgctagat tccgatggag ccacccgcag ttcgagaaag
     4501 gtggaggttc cggaggtgga tcgggaggta gtgcttggtc tcatcctcaa ttcgagaagc
     4561 tggaagttct gttccagggg cccatggaca aagattgcga aatgaaacgt accaccctgg
     4621 atagcccgct gggcaaactg gaactgagcg gctgcgaaca gggcctgcat gaaattaaac
     4681 tgctgggtaa aggcaccagc gcggccgatg cggttgaagt tccggccccg gccgccgtgc
     4741 tgggtggtcc ggaaccgctg atgcaggcga ccgcgtggct gaacgcgtat tttcatcagc
     4801 cggaagcgat tgaagaattt ccggttccgg cgctgcatca tccggtgttt cagcaggaga
     4861 gctttacccg tcaggtgctg tggaaactgc tgaaagtggt taaatttggc gaagtgatta
     4921 gctatcagca gctggcggcc ctggcgggta atccggcggc caccgccgcc gttaaaaccg
     4981 cgctgagcgg taacccggtg ccgattctga ttccgtgcca tcgtgtggtt agctctagcg
     5041 gtgcggttgg cggttatgaa ggtggtctgg cggtgaaaga gtggctgctg gcccatgaag
     5101 gtcatcgtct gggtaaaccg ggtctgggag gtagcggtag cggtagcgag actgtccgat
     5161 tccaatctgg cttattcaca gtgacagtcc ctaaggaact gtacataata gagcatggca
     5221 gcaatgtgac cctggaatgc aactttgaca ctggaagtca tgtgaacctt ggagcaataa
     5281 cagccagttt gcaaaaggtg gaaaatgata catccccaca ccgtgaaaga gccactttgc
     5341 tggaggagca gctgccccta gggaaggcct cgttccacat acctcaagtc caagtgaggg
     5401 acgaaggaca gtaccaatgc ataatcatct atggggtcgc ctgggactac aagtacctga
     5461 ctctgaaagt caaagcttcc tacaggaaaa taaacactca catcctaaag gttccagaaa
     5521 cagatgaggt agagctcacc tgccaggcta caggttatcc tctggcagaa gtatcctggc
     5581 caaacgtcag cgttcctgcc aacaccagcc actccaggac ccctgaaggc ctctaccagg
     5641 tcaccagtgt tctgcgccta aagccacccc ctggcagaaa cttcagctgt gtgttctgga
     5701 atactcacgt gagggaactt actttggcca gcattgacct tcaaagtcag atggaaccca
     5761 ggacccatcc aggccatcac catcaccatc accatcacca tcactgagcg gccgctcgag
     5821 catgcatcta gagcttgcgc ctctagagct cgctgatcag cctcgactgt gccttctagt
     5881 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5941 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6001 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6061 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     6121 tcgagggggg atctcccgat ccccagcttt gcttctcaat ttcttatttg cataatgaga
     6181 aaaaaaggaa aattaatttt aacaccaatt cagtagttga ttgagcaaat gcgttgccaa
     6241 aaaggatgct ttagagacag tgttctctgc acagataagg acaaacatta ttcagaggga
     6301 gtacccagag ctgagactcc taagccagtg agtggcacag catccaggga gaaatatgct
     6361 tgtcatcacc gaagcctgat tccgtagagc cacaccctgg taagggccaa tctgctcaca
     6421 caggatagag agggcaggag ccagggcaga gcatataagg tgaggtagga tcagttgctc
     6481 ctcacatttg cttctgacat agttgtgttg ggagcttgga tagcttgggg gggggacagc
     6541 tcagggctgc gatttcgcgc caaacttgac ggcaatccta gcgtgaaggc tggtaggatt
     6601 ttatccccgc tgccatcatg gttcgaccat tgaactgcat cgtcgccgtg tcccaaaata
     6661 tggggattgg caagaacgga gacctaccct ggcctccgct caggaacgag ttcaagtact
     6721 tccaaagaat gaccacaacc tcttcagtgg aaggtaaaca gaatctggtg attatgggta
     6781 ggaaaacctg gttctccatt cctgagaaga atcgaccttt aaaggacaga attaatatag
     6841 ttctcagtag agaactcaaa gaaccaccac gaggagctca ttttcttgcc aaaagtttgg
     6901 atgatgcctt aagacttatt gaacaaccgg aattggcaag taaagtagac atggtttgga
     6961 tagtcggagg cagttctgtt taccaggaag ccatgaatca acctcagact ctttgtgaca
     7021 aggatcatgc aggaatttga aagtgacacg tttttcccag aaattgattt ggggaaatat
     7081 aaacttctcc cagaataccc aggcgtcctc tctgaggtcc aggaggaaaa aggcatcaag
     7141 tataagtttg aagtctacga gaagaaagac taacaggaag atgctttcaa gttctctgct
     7201 cccctcctaa agctatgcat ttttataaga ccatgctagc ttgaacttgt ttattgcagc
     7261 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7321 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     7381 cgatagctta tctgtgggcg atgccaagca cctggatgct gttggtttcc tgctactgat
     7441 ttagaagcca tttgccccct gagtggggct tgggagcact aactttctct ttcaaaggaa
     7501 gcaatgcaga aagaaaagca tacaaagtat aagctgccat gtaataatgg aagaagataa
     7561 ggttgtatga attagattta catacttctg aattgaaact aaacaccttt aaattcttaa
     7621 atatataaca catttcatat gaaagtattt tacataagta actcagatac atagaaaaca
     7681 aagctaatga taggtgtccc taaaagttca tttattaatt ctacaaatga tgagctggcc
     7741 atcaaaattc cagctcaatt cttcaacgaa ttagaaagag caatctgcaa actcatctgg
     7801 aataacaaaa aacctaggat agcaaaaact cttctcaagg ataaaagaac ctctggtgga
     7861 atcaccatgc ctgacctaaa gctgtactac agagcaattg tgataaaaac tgcatggtac
     7921 tgatatagaa acggacaagt agaccaatgg aatagaaccc acacacctat ggtcacttga
     7981 tcttcaacaa gagagctaaa accatccact ggaaa
//
