LOCUS       Exported                8000 bp ds-DNA     linear   UNA 19-DEC-2018
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 5, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    4377..4382
                     /locus_tag="PstI"
                     /label=PstI
     misc_feature    4383..4475
                     /locus_tag="Signal Peptide"
                     /label=Signal Peptide
     misc_feature    4476..4559
                     /locus_tag="twinstrep(codon optimized)"
                     /label=twinstrep(codon optimized)
     misc_feature    4560..4583
                     /locus_tag="preSci recognition site"
                     /label=preSci recognition site
     misc_feature    4584..5129
                     /locus_tag="SNAP tag"
                     /label=SNAP tag
     misc_feature    5130..5147
                     /locus_tag="(GS)3"
                     /label=(GS)3
     misc_feature    5148..5168
                     /locus_tag="TVMV site"
                     /label=TVMV site
     misc_feature    5169..5171
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    5172..5795
                     /locus_tag="hB7.1extracellular domain(35-242aa)"
                     /label=hB7.1extracellular domain(35-242aa)
     misc_feature    5796..5798
                     /locus_tag="STOP"
                     /label=STOP
     misc_feature    5799..5800
                     /locus_tag="NotI"
                     /label=NotI
ORIGIN
        1 aaagacagca ttttcaacaa atggtgctgg cacaactggt ggttatcatg gagaagaatg
       61 tgaattgatc cattccaatc tccttgtact aaggtcaaat ctaagtggat caaggaactc
      121 cacataaaac cagagacact gaaacttata gaggagaaag tggggaaaag cctcgaagat
      181 atgggcacag gggaaaaatt cctgaataga acagcaatgg cttgtgctgt aagatcgaga
      241 attgacaaat gggacctcat gaaactccaa agctatcgga tcaattcctc caaaaaagcc
      301 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
      361 aaaaattagt cagccatgca tggggcggag aatgggcgga actgggcgga gttaggggcg
      421 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
      481 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
      541 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
      601 acacattcca cagaattaat tcccgatccg tcgacctcga gagcttggcg taatcatggt
      661 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      721 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      781 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      841 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgata tccgcttcct cgctcactga
      901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1621 gattacgcgc agaaaaaaag gatctcaaga agataccttt gatatttata cggggtctga
     1681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1861 tctaaacgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1921 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1981 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2041 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2101 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2161 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2221 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2281 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2341 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2401 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2461 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2521 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2581 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2641 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2701 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2761 aataaacaaa taggggttcc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     2821 atcgggagat ccgcgttgac attgattatt gactagttat taatagtaat caattacggg
     2881 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2941 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     3001 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     3061 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     3121 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     3181 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     3241 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3301 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     3361 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3421 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     3481 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
     3541 ccgtgccaag agtgacgtaa gtaccgccta tagactctat aggcacaccc ctttggctct
     3601 tatgcatgct atactgtttt tggcttgggg cctatacacc cccgcttcct tatgctatag
     3661 gtgatggtat agcttagcct ataggtgtgg gttattgacc attattgacc actcccctat
     3721 tggtgacgat actttccatt actaatccat aacatggctc tttgccacaa ctatctctat
     3781 tggctatatg ccaatactct gtccttcaga gactgacacg gactctgtat ttttacagga
     3841 tggggtccca tttattattt acaaattcac atatacaaca acgccgtccc ccgtgcccgc
     3901 agtttttatt aaacatagcg tgggatctcc acgcgaatct cgggtacgtg ttccggacat
     3961 gggctcttct ccggtagcgg cggagcttcc acatccgagc cctggtccca tgcctccagc
     4021 ggctcatggt cgctcggcag ctccttgctc ctaacagtgg aggccagact taggcacagc
     4081 acaatgccca ccaccaccag tgtgccgcac aaggccgtgg cggtagggta tgtgtctgaa
     4141 aatgagctcg gagattgggc tcgcaccgct gacgcagatg gaagacttaa ggcagcggca
     4201 gaagaagatg caggcagctg agttgttgta ttctgataag agtcagaggt aactcccgtt
     4261 gcggtgctgt taacggtgga gggcagtgta gtctgagcag tactcgttgc tgccgcgcgc
     4321 gccaccagac ataatagctg acagactaac agactgttcc tttccatggg tcttttctgc
     4381 agatggacgc tatgaagaga ggactgtgct gcgtgctgct gctgtgcgga gccgtgttcg
     4441 tgtcaccaag ccaggaaatc catgctagat tccgatggag ccacccgcag ttcgagaaag
     4501 gtggaggttc cggaggtgga tcgggaggta gtgcttggtc tcatcctcaa ttcgagaagc
     4561 tggaagttct gttccagggg cccatggaca aagattgcga aatgaaacgt accaccctgg
     4621 atagcccgct gggcaaactg gaactgagcg gctgcgaaca gggcctgcat gaaattaaac
     4681 tgctgggtaa aggcaccagc gcggccgatg cggttgaagt tccggccccg gccgccgtgc
     4741 tgggtggtcc ggaaccgctg atgcaggcga ccgcgtggct gaacgcgtat tttcatcagc
     4801 cggaagcgat tgaagaattt ccggttccgg cgctgcatca tccggtgttt cagcaggaga
     4861 gctttacccg tcaggtgctg tggaaactgc tgaaagtggt taaatttggc gaagtgatta
     4921 gctatcagca gctggcggcc ctggcgggta atccggcggc caccgccgcc gttaaaaccg
     4981 cgctgagcgg taacccggtg ccgattctga ttccgtgcca tcgtgtggtt agctctagcg
     5041 gtgcggttgg cggttatgaa ggtggtctgg cggtgaaaga gtggctgctg gcccatgaag
     5101 gtcatcgtct gggtaaaccg ggtctgggag gtagcggtag cggtagcgag actgtccgat
     5161 tccaatctgg cgttatccac gtgaccaagg aagtgaaaga agtggcaacg ctgtcctgtg
     5221 gtcacaatgt ttctgttgaa gagctggcac aaactcgcat ctactggcaa aaggagaaga
     5281 aaatggtgct gactatgatg tctggggaca tgaatatatg gcccgagtac aagaaccgga
     5341 ccatctttga tatcactaat aacctctcca ttgtgatcct ggctctgcgc ccatctgacg
     5401 agggcacata cgagtgtgtt gttctgaagt atgaaaaaga cgctttcaag cgggaacacc
     5461 tggctgaagt gacgttatca gtcaaagctg acttccctac acctagtata tctgactttg
     5521 aaattccaac ttctaatatt agaaggataa tttgctcaac ctctggaggt tttccagagc
     5581 ctcacctctc ctggttggaa aatggagaag aattaaatgc catcaacaca acagtttccc
     5641 aagatcctga aactgagctc tatgctgtta gcagcaaact ggatttcaat atgacaacca
     5701 accacagctt catgtgtctc atcaagtatg gacatttaag agtgaatcag accttcaact
     5761 ggaatacaac caagcaagag cattttcctg ataactgagc tcgagcatgc atctagagct
     5821 tgcgcctcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5881 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5941 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6001 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6061 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag gggggatctc
     6121 ccgatcccca gctttgcttc tcaatttctt atttgcataa tgagaaaaaa aggaaaatta
     6181 attttaacac caattcagta gttgattgag caaatgcgtt gccaaaaagg atgctttaga
     6241 gacagtgttc tctgcacaga taaggacaaa cattattcag agggagtacc cagagctgag
     6301 actcctaagc cagtgagtgg cacagcatcc agggagaaat atgcttgtca tcaccgaagc
     6361 ctgattccgt agagccacac cctggtaagg gccaatctgc tcacacagga tagagagggc
     6421 aggagccagg gcagagcata taaggtgagg taggatcagt tgctcctcac atttgcttct
     6481 gacatagttg tgttgggagc ttggatagct tggggggggg acagctcagg gctgcgattt
     6541 cgcgccaaac ttgacggcaa tcctagcgtg aaggctggta ggattttatc cccgctgcca
     6601 tcatggttcg accattgaac tgcatcgtcg ccgtgtccca aaatatgggg attggcaaga
     6661 acggagacct accctggcct ccgctcagga acgagttcaa gtacttccaa agaatgacca
     6721 caacctcttc agtggaaggt aaacagaatc tggtgattat gggtaggaaa acctggttct
     6781 ccattcctga gaagaatcga cctttaaagg acagaattaa tatagttctc agtagagaac
     6841 tcaaagaacc accacgagga gctcattttc ttgccaaaag tttggatgat gccttaagac
     6901 ttattgaaca accggaattg gcaagtaaag tagacatggt ttggatagtc ggaggcagtt
     6961 ctgtttacca ggaagccatg aatcaacctc agactctttg tgacaaggat catgcaggaa
     7021 tttgaaagtg acacgttttt cccagaaatt gatttgggga aatataaact tctcccagaa
     7081 tacccaggcg tcctctctga ggtccaggag gaaaaaggca tcaagtataa gtttgaagtc
     7141 tacgagaaga aagactaaca ggaagatgct ttcaagttct ctgctcccct cctaaagcta
     7201 tgcattttta taagaccatg ctagcttgaa cttgtttatt gcagcttata atggttacaa
     7261 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7321 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaacgata gcttatctgt
     7381 gggcgatgcc aagcacctgg atgctgttgg tttcctgcta ctgatttaga agccatttgc
     7441 cccctgagtg gggcttggga gcactaactt tctctttcaa aggaagcaat gcagaaagaa
     7501 aagcatacaa agtataagct gccatgtaat aatggaagaa gataaggttg tatgaattag
     7561 atttacatac ttctgaattg aaactaaaca cctttaaatt cttaaatata taacacattt
     7621 catatgaaag tattttacat aagtaactca gatacataga aaacaaagct aatgataggt
     7681 gtccctaaaa gttcatttat taattctaca aatgatgagc tggccatcaa aattccagct
     7741 caattcttca acgaattaga aagagcaatc tgcaaactca tctggaataa caaaaaacct
     7801 aggatagcaa aaactcttct caaggataaa agaacctctg gtggaatcac catgcctgac
     7861 ctaaagctgt actacagagc aattgtgata aaaactgcat ggtactgata tagaaacgga
     7921 caagtagacc aatggaatag aacccacaca cctatggtca cttgatcttc aacaagagag
     7981 ctaaaaccat ccactggaaa
//
