LOCUS       Exported                7673 bp ds-DNA     circular UNA 25-JUL-2019
DEFINITION  natural linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 5, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    4377..4382
                     /locus_tag="PstI"
                     /label=PstI
     misc_feature    4383..4475
                     /locus_tag="Signal Peptide"
                     /label=Signal Peptide
     misc_feature    4476..5021
                     /locus_tag="SNAP tag"
                     /label=SNAP tag
     misc_feature    5022..5039
                     /locus_tag="(GS)3"
                     /label=(GS)3
     misc_feature    5040..5060
                     /locus_tag="TVMV site"
                     /label=TVMV site
     misc_feature    5061..5063
                     /locus_tag="EcoRI"
                     /label=EcoRI
     exon            5064..5441
                     /locus_tag="CTLA4((36-161aa)"
                     /label=CTLA4((36-161aa)
     misc_feature    5442..5444
                     /locus_tag="EcoRI(1)"
                     /label=EcoRI
     misc_feature    5445..5462
                     /locus_tag="His10"
                     /label=His10
     misc_feature    5463..5465
                     /locus_tag="STOP"
                     /label=STOP
     misc_feature    5466..5473
                     /locus_tag="NotI"
                     /label=NotI
ORIGIN
        1 aaagacagca ttttcaacaa atggtgctgg cacaactggt ggttatcatg gagaagaatg
       61 tgaattgatc cattccaatc tccttgtact aaggtcaaat ctaagtggat caaggaactc
      121 cacataaaac cagagacact gaaacttata gaggagaaag tggggaaaag cctcgaagat
      181 atgggcacag gggaaaaatt cctgaataga acagcaatgg cttgtgctgt aagatcgaga
      241 attgacaaat gggacctcat gaaactccaa agctatcgga tcaattcctc caaaaaagcc
      301 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
      361 aaaaattagt cagccatgca tggggcggag aatgggcgga actgggcgga gttaggggcg
      421 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
      481 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
      541 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
      601 acacattcca cagaattaat tcccgatccg tcgacctcga gagcttggcg taatcatggt
      661 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      721 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      781 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      841 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgata tccgcttcct cgctcactga
      901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1621 gattacgcgc agaaaaaaag gatctcaaga agataccttt gatatttata cggggtctga
     1681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1861 tctaaacgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1921 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1981 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2041 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2101 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2161 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2221 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2281 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2341 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2401 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2461 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2521 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2581 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2641 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2701 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2761 aataaacaaa taggggttcc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     2821 atcgggagat ccgcgttgac attgattatt gactagttat taatagtaat caattacggg
     2881 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2941 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     3001 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     3061 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     3121 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     3181 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     3241 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3301 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     3361 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3421 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     3481 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
     3541 ccgtgccaag agtgacgtaa gtaccgccta tagactctat aggcacaccc ctttggctct
     3601 tatgcatgct atactgtttt tggcttgggg cctatacacc cccgcttcct tatgctatag
     3661 gtgatggtat agcttagcct ataggtgtgg gttattgacc attattgacc actcccctat
     3721 tggtgacgat actttccatt actaatccat aacatggctc tttgccacaa ctatctctat
     3781 tggctatatg ccaatactct gtccttcaga gactgacacg gactctgtat ttttacagga
     3841 tggggtccca tttattattt acaaattcac atatacaaca acgccgtccc ccgtgcccgc
     3901 agtttttatt aaacatagcg tgggatctcc acgcgaatct cgggtacgtg ttccggacat
     3961 gggctcttct ccggtagcgg cggagcttcc acatccgagc cctggtccca tgcctccagc
     4021 ggctcatggt cgctcggcag ctccttgctc ctaacagtgg aggccagact taggcacagc
     4081 acaatgccca ccaccaccag tgtgccgcac aaggccgtgg cggtagggta tgtgtctgaa
     4141 aatgagctcg gagattgggc tcgcaccgct gacgcagatg gaagacttaa ggcagcggca
     4201 gaagaagatg caggcagctg agttgttgta ttctgataag agtcagaggt aactcccgtt
     4261 gcggtgctgt taacggtgga gggcagtgta gtctgagcag tactcgttgc tgccgcgcgc
     4321 gccaccagac ataatagctg acagactaac agactgttcc tttccatggg tcttttctgc
     4381 agatggacgc tatgaagaga ggactgtgct gcgtgctgct gctgtgcgga gccgtgttcg
     4441 tgtcaccaag ccaggaaatc catgctagat tccgaatgga caaagattgc gaaatgaaac
     4501 gtaccaccct ggatagcccg ctgggcaaac tggaactgag cggctgcgaa cagggcctgc
     4561 atgaaattaa actgctgggt aaaggcacca gcgcggccga tgcggttgaa gttccggccc
     4621 cggccgccgt gctgggtggt ccggaaccgc tgatgcaggc gaccgcgtgg ctgaacgcgt
     4681 attttcatca gccggaagcg attgaagaat ttccggttcc ggcgctgcat catccggtgt
     4741 ttcagcagga gagctttacc cgtcaggtgc tgtggaaact gctgaaagtg gttaaatttg
     4801 gcgaagtgat tagctatcag cagctggcgg ccctggcggg taatccggcg gccaccgccg
     4861 ccgttaaaac cgcgctgagc ggtaacccgg tgccgattct gattccgtgc catcgtgtgg
     4921 ttagctctag cggtgcggtt ggcggttatg aaggtggtct ggcggtgaaa gagtggctgc
     4981 tggcccatga aggtcatcgt ctgggtaaac cgggtctggg aggtagcggt agcggtagcg
     5041 agactgtccg attccaatct gaaaaagcaa tgcacgtggc ccagcctgct gtggtactgg
     5101 ccagcagccg aggcatcgcc agctttgtgt gtgagtatgc atctccaggc aaagccactg
     5161 aggtccgggt gacagtgctt cggcaggctg acagccaggt gactgaagtc tgtgcggcaa
     5221 cctacatgat ggggaatgag ttgaccttcc tagatgattc catctgcacg ggcacctcca
     5281 gtggaaatca agtgaacctc actatccaag gactgagggc catggacacg ggactctaca
     5341 tctgcaaggt ggagctcatg tacccaccgc catactacct gggcataggc aacggaaccc
     5401 agatttatgt aattgatcca gaaccgtgcc cagattctga cggccatcac catcaccatc
     5461 actgagcggc cgctcgagca tgcatctaga gcttgcgcct ctagagctcg ctgatcagcc
     5521 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5581 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5641 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5701 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5761 gaaagaacca gctggggctc gaggggggat ctcccgatcc ccagctttgc ttctcaattt
     5821 cttatttgca taatgagaaa aaaaggaaaa ttaattttaa caccaattca gtagttgatt
     5881 gagcaaatgc gttgccaaaa aggatgcttt agagacagtg ttctctgcac agataaggac
     5941 aaacattatt cagagggagt acccagagct gagactccta agccagtgag tggcacagca
     6001 tccagggaga aatatgcttg tcatcaccga agcctgattc cgtagagcca caccctggta
     6061 agggccaatc tgctcacaca ggatagagag ggcaggagcc agggcagagc atataaggtg
     6121 aggtaggatc agttgctcct cacatttgct tctgacatag ttgtgttggg agcttggata
     6181 gcttgggggg gggacagctc agggctgcga tttcgcgcca aacttgacgg caatcctagc
     6241 gtgaaggctg gtaggatttt atccccgctg ccatcatggt tcgaccattg aactgcatcg
     6301 tcgccgtgtc ccaaaatatg gggattggca agaacggaga cctaccctgg cctccgctca
     6361 ggaacgagtt caagtacttc caaagaatga ccacaacctc ttcagtggaa ggtaaacaga
     6421 atctggtgat tatgggtagg aaaacctggt tctccattcc tgagaagaat cgacctttaa
     6481 aggacagaat taatatagtt ctcagtagag aactcaaaga accaccacga ggagctcatt
     6541 ttcttgccaa aagtttggat gatgccttaa gacttattga acaaccggaa ttggcaagta
     6601 aagtagacat ggtttggata gtcggaggca gttctgttta ccaggaagcc atgaatcaac
     6661 ctcagactct ttgtgacaag gatcatgcag gaatttgaaa gtgacacgtt tttcccagaa
     6721 attgatttgg ggaaatataa acttctccca gaatacccag gcgtcctctc tgaggtccag
     6781 gaggaaaaag gcatcaagta taagtttgaa gtctacgaga agaaagacta acaggaagat
     6841 gctttcaagt tctctgctcc cctcctaaag ctatgcattt ttataagacc atgctagctt
     6901 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6961 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7021 ttatcatgtc tggatcaacg atagcttatc tgtgggcgat gccaagcacc tggatgctgt
     7081 tggtttcctg ctactgattt agaagccatt tgccccctga gtggggcttg ggagcactaa
     7141 ctttctcttt caaaggaagc aatgcagaaa gaaaagcata caaagtataa gctgccatgt
     7201 aataatggaa gaagataagg ttgtatgaat tagatttaca tacttctgaa ttgaaactaa
     7261 acacctttaa attcttaaat atataacaca tttcatatga aagtatttta cataagtaac
     7321 tcagatacat agaaaacaaa gctaatgata ggtgtcccta aaagttcatt tattaattct
     7381 acaaatgatg agctggccat caaaattcca gctcaattct tcaacgaatt agaaagagca
     7441 atctgcaaac tcatctggaa taacaaaaaa cctaggatag caaaaactct tctcaaggat
     7501 aaaagaacct ctggtggaat caccatgcct gacctaaagc tgtactacag agcaattgtg
     7561 ataaaaactg catggtactg atatagaaac ggacaagtag accaatggaa tagaacccac
     7621 acacctatgg tcacttgatc ttcaacaaga gagctaaaac catccactgg aaa
//
