LOCUS       Exported                6097 bp ds-DNA     circular SYN 15-JUN-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 5, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     PCR_frame       1441..2059 /note=""""Reading Frame"""" /set=""""Set 
            1"""" PCR_product     1441..2059 /pair=""""Pair 1"""" /set=""""Set 
            1"""" /current=1 /score=""""0.000""""      PCR_primer 
            complement(2036..2059) /primer=""""CTAACTGACACACATTCCACAGGG"""" 
            /set=""""Set 1"""" /pair=""""Pair 1"""" /current=1      PCR_primer 
            1441..1464 /primer=""""AGCCCTTTGTACACCCTAAGCCTC"""" /set=""""Set 
            1"""" /pair=""""Pair 1"""" /current=1      PCR_primer      
            1882..1900 /pair="""""""" /primer=""""CAATTGGAGAAAAGCCTTG"""" 
            /current=0 Features not parsed: PCR_primer      
            complement(1924..1945) /pair="""""""" 
            /primer=""""GCTAGGATCCTAGCTCTAAAAC"""" /current=0 
            VNTOAUTHORNAME|Johannes Zuber| VNTAUTHORNAME|jp| 
            VNTNAME|pQCX/mU6-SV-Cas9-iG| LSOWNER| VNTDBDATE|661680742| 
            VNTDATE|661624011|
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..2040
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:synthetic DNA construct"
     source          2706..6097
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /label=source:synthetic DNA construct(1)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:synthetic DNA construct(1)"
     LTR             1..728
                     /locus_tag="5'LTR CMV/MSV"
                     /label=5'LTR CMV/MSV
     misc_feature    758..1567
                     /locus_tag="Psi+"
                     /label=Psi+
                     /note="/dnas_title=""Psi+"
                     /note="/vntifkey=""21"""
     misc_feature    1152..1568
                     /locus_tag="Gag"
                     /label=Gag
     primer_bind     1152..1173
                     /locus_tag="Gag Seq Primer"
                     /label=Gag Seq Primer
     primer_bind     1441..1461
                     /locus_tag="Psi Sequencing Primer"
                     /label=Psi Sequencing Primer
                     /note="/dnas_title=""Psi Sequencing Primer"
                     /note="/vntifkey=""27"""
     promoter        1628..1896
                     /locus_tag="SV40 Promoter"
                     /label=SV40 Promoter
                     /note="/dnas_title=""SV40 Promoter"
                     /note="/vntifkey=""29"""
     misc_feature    1948..1967
                     /locus_tag="T7 RNA Polymerase Binding Site"
                     /label=T7 RNA Polymerase Binding Site
                     /note="/dnas_title=""T7 RNA Polymerase Binding Site"
                     /note="/vntifkey=""21"""
     misc_feature    1948..1966
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
     misc_feature    1978..2034
                     /locus_tag="Xenopus b-globin 5'UTR"
                     /label=Xenopus b-globin 5'UTR
                     /note="/dnas_title=""Xenopus b-globin 5'UTR"
                     /note="/vntifkey=""21"""
     5'UTR           1981..2023
                     /locus_tag="Xenopus globin 5'-UTR"
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(2004..2040)
                     /locus_tag="EH-3927"
                     /label=EH-3927
     primer_bind     complement(2007..2040)
                     /locus_tag="rv_VectorRev"
                     /label=rv_VectorRev
     misc_feature    2018..2040
                     /locus_tag="Gene Block PD1Hu_cDNA"
                     /label=Gene Block PD1Hu_cDNA
     primer_bind     2018..2040
                     /locus_tag="EH-3929"
                     /label=EH-3929
     regulatory      2035..2040
                     /regulatory_class="other"
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     misc_feature    2041..2697
                     /locus_tag="mCD28"
                     /label=mCD28
     misc_feature    2605..2607
                     /locus_tag="Y170F"
                     /label=Y170F
     CDS             2698..2705
                     /locus_tag="mThy1.1"
                     /label=mThy1.1
     LTR             2803..3228
                     /locus_tag="SIN-LTR"
                     /label=SIN-LTR
                     /note="/dnas_title=""SIN-LTR"
                     /note="/vntifkey=""19"""
     primer_bind     2803..2825
                     /locus_tag="SIN3'LTR seq primer"
                     /label=SIN3'LTR seq primer
     CDS             4875..5735
                     /codon_start=1
                     /locus_tag="AmpR"
                     /label=AmpR
                     /translation="LPMLNQ*GTYLSDLSISFIHSCLTPRRVDNYDTGGLTIWPQCCND
                     TARPTLTGSRFISNKPASRKGRAQKWSCNFIRLHPVY*LLPGS*SK*FAS**FAQRCCH
                     CCRHRGVTLVVWYGFIQLRFPTIKASYMIPHVVQKSG*LLRSSDRCQK*VGRSVITHGY
                     GSTA*FSYCHAIRKMLFCDW*VLNQVILRIVYAATELLLPGVNTG*YRAT*QNFKSAHH
                     WKTFFGAKTLKDLTAVEIQFDVTHSCTQLIFSIFYFHQRFWVSKNRKAKCRKKGNKGDT
                     EMLNTH"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
      541 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
      601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
      721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
      781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
      841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
     1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
     1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1561 taggcgccgg aattgaagat ctcccaacca gcacagtggt cgaccctgtg gaatgtgtgt
     1621 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     1681 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     1741 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1801 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1861 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1921 tttggaggat cttaaggcta gagtacttaa tacgactcac tataggggca aacaaaattg
     1981 cttgttcttt ttgcagaagc tcagaataaa cgctcaactt tggcagatac gcgtgccacc
     2041 atgacactca ggctgctgtt cttggctctc aacttcttct cagttcaagt aacagaaaac
     2101 aagattttgg taaagcagtc gcccctgctt gtggtagata gcaacgaggt cagcctcagc
     2161 tgcaggtatt cctacaacct tctcgcaaag gaattccggg catccctgta caagggcgtg
     2221 aacagcgacg tggaagtctg tgtcgggaat gggaatttta cctatcagcc ccagtttcgc
     2281 tcgaatgccg agttcaactg cgacggggat ttcgacaacg aaacagtgac gttccgtctc
     2341 tggaatctgc acgtcaatca cacagatatt tacttctgca aaattgagtt catgtaccct
     2401 ccgccttacc tagacaacga gaggagcaat ggaactatta ttcacataaa agagaaacat
     2461 ctttgtcata ctcagtcatc tcctaagctg ttttgggcac tggtcgtggt tgctggagtc
     2521 ctgttttgtt atggcttgct agtgacagtg gctctttgtg ttatctggac aaatagtaga
     2581 aggaacagac tccttcaaag tgacttcatg aacatgactc cccggaggcc tgggctcact
     2641 cgaaagcctt accagcccta cgcccctgcc agagactttg cagcgtaccg cccctaagcg
     2701 gccgcatctt aattaagaat tatcaagctt atcgctgaag cctatagagt acgagccata
     2761 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     2821 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     2881 gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga ccctagagaa
     2941 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     3001 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     3061 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc
     3121 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     3181 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc
     3241 cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg
     3301 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3361 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3421 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtagat
     3481 ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3541 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3601 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3661 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3721 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3781 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta ctggcttaac
     3841 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     3901 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     3961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     4561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4861 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4921 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4981 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5041 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5101 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5161 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     5221 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5281 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5341 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5401 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5461 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     5521 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5581 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5641 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5701 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     5881 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     5941 gaattagctt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
     6001 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     6061 attacggggt cattagttca tagcccatat atggagt
//
