LOCUS       pUC18.1rp_ipgC_t        4192 bp DNA     circular SYN 02-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA
[LOCUS]: Exported
[ACCESSION]: 
            .
[SOURCE]: 1..2701
[CDS]: 399..1058
FEATURES             Location/Qualifiers
     source          1..4192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             399..1058
                     /label=AmpR
                     /note="label=AmpR - 399..1058"
     rep_origin      1210..1838
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="direction=RIGHT - label=ColE1 origin - 1210..1838"
     misc_feature    2124..2202
                     /label=rpsM promoter
                     /note="label=rpsM promoter - 2124..2202"
     misc_feature    2288..2749
                     /label=IpgC
     CDS             2363..2431
                     /label=LacZ alpha
                     /note="label=LacZ alpha - 2395..2463"
     misc_feature    2765..3721
                     /label=TurboID
     misc_feature    3728..3757
                     /label=Myc tag
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctttgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcgattttt tcgcatattt ttcttgcaaa gttgggttga
     2161 gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtacaggaaa cagctatgac
     2221 catgtaacta gctaactcga gtaactagct aagattacga attctaggag gtgctagcgc
     2281 tatgggatct ttaaatatca ccgaaaatga aagcatctct actgcagtaa ttgatgcaat
     2341 taactctggc gctacactga aagatattaa tgcaattcct gatgatatga tggatgacat
     2401 ttattcatat gcttatgact tttacaacaa aggaagaata gaggaagctg aagttttctt
     2461 caggttttta tgtatatacg acttttacaa tgtagactac attatgggac tcgcagctat
     2521 ttatcagata aaagaacagt tccaacaagc agcagacctt tatgctgtcg cttttgcatt
     2581 aggaaaaaat gactatacac cagtattcca tactggacaa tgtcagcttc ggttgaaagc
     2641 ccccttaaaa gctaaagagt gcttcgaact cgtaattcaa cacagcaatg atgaaaaatt
     2701 aaaaataaaa gcacaatcat acttggacgc aattcaggat atcaaggagg gatccggcgg
     2761 tggcaaagac aatactgtgc ctctgaagct gatcgctctc ctggctaatg gcgagttcca
     2821 tagtggcgaa cagctgggag aaaccctggg catgtccagg gccgctatca acaagcacat
     2881 tcagactctg cgcgactggg gcgtggacgt gttcaccgtg cccggaaagg gctactctct
     2941 gcccgagcct atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag
     3001 cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga
     3061 gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg
     3121 gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa
     3181 gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct
     3241 gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga
     3301 tagaaagctg gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat
     3361 cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca
     3421 gggctggatc acactgcagg aagcagggat taacctggac aggaatactc tggccgctac
     3481 gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata
     3541 tctgccacgg tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg
     3601 ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga
     3661 acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa
     3721 gtccggagaa cagaagctta ttagcgaaga ggatctgtaa ctaatctaga gtcgacctgc
     3781 aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3841 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3901 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     3961 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4021 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4081 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4141 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc ga
//
