LOCUS       pUC18.1rp_turboI        4195 bp DNA     circular SYN 02-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA
[LOCUS]: Exported
[ACCESSION]: 
            .
[SOURCE]: 1..2701
[CDS]: 399..1058
FEATURES             Location/Qualifiers
     source          1..4195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..159
                     /label=Amp prom
                     /note="label=Amp prom - 131..159"
     CDS             399..1058
                     /label=AmpR
                     /note="label=AmpR - 399..1058"
     rep_origin      1210..1838
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="direction=RIGHT - label=ColE1 origin - 1210..1838"
     misc_feature    2124..2202
                     /label=rpsM promoter
                     /note="label=rpsM promoter - 2124..2202"
     misc_feature    2282..3241
                     /label=TurboID
     misc_feature    3248..3277
                     /label=Myc tag
     misc_feature    3284..3745
                     /label=ipgC
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctttgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcgattttt tcgcatattt ttcttgcaaa gttgggttga
     2161 gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtacaggaaa cagctatgac
     2221 catgtaacta gctaactcga gtaactagct aagattacga attctaggag gtgctagcgc
     2281 tatgaaagac aatactgtgc ctctgaagct gatcgctctc ctggctaatg gcgagttcca
     2341 tagtggcgaa cagctgggag aaaccctggg catgtccagg gccgctatca acaagcacat
     2401 tcagactctg cgcgactggg gcgtggacgt gttcaccgtg cccggaaagg gctactctct
     2461 gcccgagcct atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag
     2521 cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga
     2581 gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg
     2641 gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa
     2701 gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct
     2761 gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga
     2821 tagaaagctg gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat
     2881 cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca
     2941 gggctggatc acactgcagg aagcagggat taacctggac aggaatactc tggccgctac
     3001 gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata
     3061 tctgccacgg tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg
     3121 ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga
     3181 acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa
     3241 gtccggagaa cagaagctta ttagcgaaga ggatctgggt acctctttaa atatcaccga
     3301 aaatgaaagc atctctactg cagtaattga tgcaattaac tctggcgcta cactgaaaga
     3361 tattaatgca attcctgatg atatgatgga tgacatttat tcatatgctt atgactttta
     3421 caacaaagga agaatagagg aagctgaagt tttcttcagg tttttatgta tatacgactt
     3481 ttacaatgta gactacatta tgggactcgc agctatttat cagataaaag aacagttcca
     3541 acaagcagca gacctttatg ctgtcgcttt tgcattagga aaaaatgact atacaccagt
     3601 attccatact ggacaatgtc agcttcggtt gaaagccccc ttaaaagcta aagagtgctt
     3661 cgaactcgta attcaacaca gcaatgatga aaaattaaaa ataaaagcac aatcatactt
     3721 ggacgcaatt caggatatca aggagtaaga gctcggatcc taactaatct agagtcgacc
     3781 tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3841 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3901 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     3961 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     4021 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4081 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4141 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcga
//
