LOCUS       pUC18.1rp_mxiE_t        4475 bp DNA     circular SYN 02-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA
[LOCUS]: Exported
[ACCESSION]: 
            .
[SOURCE]: 1..2701
[CDS]: 399..1058
FEATURES             Location/Qualifiers
     source          1..4475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             399..1058
                     /label=AmpR
                     /note="label=AmpR - 399..1058"
     rep_origin      1210..1838
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="direction=RIGHT - label=ColE1 origin - 1210..1838"
     misc_feature    2124..2202
                     /label=rpsM promoter
                     /note="label=rpsM promoter - 2124..2202"
     misc_feature    2265..3032
                     /label=MxiE
     CDS             2363..2431
                     /label=LacZ alpha
                     /note="label=LacZ alpha - 2395..2463"
     misc_feature    3048..4004
                     /label=TurboID
     misc_feature    4011..4040
                     /label=Myc tag
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctttgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcgattttt tcgcatattt ttcttgcaaa gttgggttga
     2161 gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtacaggaaa cagctatgac
     2221 catgtaacta gctaactcga gtaactagct aagattacga attcatggga tcaagtaaat
     2281 ataaaggtct aaatacaagt aatatgtttt acatttactc tagtggacat gaaccagtta
     2341 acgttgagct tgtaaaagat aaagaacgta acataattga actggctcca gcatggaaag
     2401 gcttcttctt cgtccgaaat caaaatataa aattcagtga taatgtgaac tatcattatc
     2461 gctttaacat taatagttgt gcaaaatttc ttgctttttg ggactatttc agtggtgctc
     2521 tagtagagca tagccatgct gaaaaatgta ttcatttcta tcatgagaat gatcttcggg
     2581 atagttgtaa taccgaatcg atgcttgata agttaatgtt aagattcatc ttttcatctg
     2641 accagaatgt aagtaatgca ctggctatga tacgaatgac tgagtcatat catttggttc
     2701 tttatcttct gagaactatt gagaaagaaa aggaggtaag aataaagagt ctgacagagc
     2761 attatggtgt gtcggaggct tattttagat ctttatgtag aaaagcttta ggggcaaaag
     2821 tcaaagaaca actgaacact tggcggttag taaatggttt attagatgta tttttgcaca
     2881 atcaaacaat cacttcagct gccatgaata atgggtatgc atcaacatcc catttttcaa
     2941 atgagataaa aacacgacta ggcttttctg caagagaatt atcgaatatt acttttttag
     3001 tgaaaaaaat aaatgaaaaa attgggctcg agggatccgg cggtggcaaa gacaatactg
     3061 tgcctctgaa gctgatcgct ctcctggcta atggcgagtt ccatagtggc gaacagctgg
     3121 gagaaaccct gggcatgtcc agggccgcta tcaacaagca cattcagact ctgcgcgact
     3181 ggggcgtgga cgtgttcacc gtgcccggaa agggctactc tctgcccgag cctatcccgc
     3241 tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca gtcctgcctg
     3301 tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag agtggggatg
     3361 cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa tggttctctc
     3421 cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa
     3481 tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag ctgggagcag
     3541 acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag ctggcaggca
     3601 tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt ggcgccggga
     3661 ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc
     3721 aggaagcagg gattaacctg gacaggaata ctctggccgc tacgctgatc cgagagctgc
     3781 gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgcca cggtgggaga
     3841 agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa gagattttcg
     3901 ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac ggagttatca
     3961 aaccctggat gggcggagaa atcagtctgc ggtctgccga aaagtccgga gaacagaagc
     4021 ttattagcga agaggatctg taactaatct agagtcgacc tgcaggcatg caagcttggc
     4081 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4141 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4201 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     4261 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     4321 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4381 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4441 tcagaggttt tcaccgtcat caccgaaacg cgcga
//
