LOCUS       pQE80L_BioID        5702 bp DNA     circular SYN 02-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    127..144
                     /label=6HIS
     misc_feature    151..1110
                     /label=BioID
     misc_feature    1297..1953
                     /label=CmR
     misc_feature    2200..3291
                     /label=LacI
     misc_feature    3860..4488
                     /label=colE1 origin
     misc_feature    4640..5299
                     /label=AmpR
     misc_feature    5539..5567
                     /label=Amp Prom
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc aaggacaaca ccgtgcccct gaagctgatc
      181 gccctgctgg ccaacggcga gttccactct ggcgagcagc tgggagagac cctgggaatg
      241 agcagagccg ccatcaacaa gcacatccag acactgagag actggggagt ggacgtgttc
      301 accgtgcctg gcaagggcta cagcctgcct gagcctatcc agctgctgaa cgccaagcag
      361 atcctgggac agctggatgg cggaagcgtg gccgtgctgc ctgtgatcga ctccaccaat
      421 cagtacctgc tggacagaat cggagagctg aagtccggcg acgcctgcat cgccgagtac
      481 cagcaggctg gcagaggagg cagaggacgg aagtggttca gcccattcgg agccaacctg
      541 tacctgtcca tgttctggag actggagcag ggacctgctg ctgccatcgg actgagtctg
      601 gtgatcggaa tcgtgatggc cgaggtgctg agaaagctgg gagccgacaa ggtgagagtg
      661 aagtggccta atgacctgta cctccaggac cgcaagctgg ctggcatcct ggtggagctg
      721 acaggcaaga caggcgatgc cgctcagatc gtgatcggag ccggaatcaa catggccatg
      781 agaagagtgg aggagagcgt ggtgaaccag ggctggatca ccctgcagga ggctggcatc
      841 aacctggacc ggaacaccct ggccgccatg ctgatcagag agctgagagc cgctctggag
      901 ctgttcgagc aggagggact ggctccttac ctgagcagat gggagaagct ggacaacttc
      961 atcaacagac ctgtgaagct gatcatcggc gacaaggaaa tcttcggcat ctccagagga
     1021 atcgacaagc agggagctct gctgctggag caggacggaa tcatcaagcc ctggatgggc
     1081 ggagaaatct ccctgagaag cgcagagaag taaggtaccc cgggtcgacc tgcagccaag
     1141 cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     1201 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagct
     1261 agcttggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1321 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1381 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1441 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1501 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1561 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1621 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1681 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1741 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     1801 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     1861 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     1921 caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
     1981 ccttaaacgc ctggggtaat gactctctag cttgaggcat caaataaaac gaaaggctca
     2041 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     2101 gacaaatccg ccctctagat tacgtgcagt cgatgataag ctgtcaaaca tgagaattgt
     2161 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2221 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2281 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     2341 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     2401 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     2461 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     2521 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     2581 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     2641 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     2701 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     2761 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     2821 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     2881 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     2941 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     3001 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     3061 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     3121 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     3181 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     3241 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     3301 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc
     3361 ggaatttcgg gcagcgttgg gtcctggcca cgggtgcgca tgatctagag ctgcctcgcg
     3421 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3481 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     3541 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     3601 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     3661 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     3721 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3781 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3841 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3901 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3961 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4021 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4081 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4141 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4201 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4261 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     4321 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4381 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4441 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4501 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4561 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4621 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4681 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4741 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4801 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4861 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4921 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4981 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5041 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5101 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5161 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5221 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5281 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5341 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5401 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5461 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5521 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5581 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     5641 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     5701 ac
//
