LOCUS       Exported                5222 bp ds-DNA     circular SYN 04-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   VB240408-1625dxq
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 5, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinant
                     plasmid and replication of the viral genome; this ITR is 
                     identical to that of the wild-type AAV2 genome."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functional
                     equivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=6d343a478711450d8e5c28ceb27e5b84"
     promoter        158..626
                     /label=SYN1
                     /note="/application_notes=Tissue specificity: Brain. Cell 
                     type
                     specificity: Mature neurons."
                     /note="/description=Human synapsin I promoter"
                     /note="/full_name=Human synapsin I promoter"
                     /note="/official_designation=SYN1"
                     /note="/uuid=6f036b2e82244653b8e92867f598e473"
     misc_feature    651..684
                     /label=Lox2272
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase;
                     can't work with other types of Lox site."
                     /note="/description=Mutated Lox site with two base 
                     substitutions
                     of LoxP"
                     /note="/official_designation=Lox2272"
                     /note="/uuid=a19dd1e203df40eb846e5102df13ffb4"
     misc_feature    717..750
                     /label=LoxP
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase."
                     /note="/description=Locus of X(cross)-over in P1"
                     /note="/official_designation=LoxP"
                     /note="/uuid=b48e25a55648467f8c98b3f68faf613f"
     CDS             complement(763..1488)
                     /label={mcherry-CAAX}
                     /note="/application_notes="
                     /note="/official_designation={mcherry-CAAX}"
                     /note="/uuid=cbe87bdc4f59452991c2f3844e4c699a"
     misc_feature    complement(1489..1494)
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=ba4f837cfff042e38e7561244b24cfa6"
     misc_feature    complement(1501..1534)
                     /label=Lox2272
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase;
                     can't work with other types of Lox site."
                     /note="/description=Mutated Lox site with two base 
                     substitutions
                     of LoxP"
                     /note="/official_designation=Lox2272"
                     /note="/uuid=991e0551b2034abd9c3ad4e364260ba6"
     misc_feature    complement(1567..1600)
                     /label=LoxP
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase."
                     /note="/description=Locus of X(cross)-over in P1"
                     /note="/official_designation=LoxP"
                     /note="/uuid=f4dc79ba31354e87bba6736ab88dbb23"
     misc_feature    1631..2228
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packaging
                     cells, leading to higher titer of packaged virus; enhances 
                     higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional
                     regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional
                     regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     polyA_signal    2259..2466
                     /label=BGH pA
                     /note="/application_notes=Allows transcription termination 
                     and
                     polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
     misc_feature    complement(2474..2603)
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinant
                     plasmid and replication of the viral genome; this ITR is 
                     identical to that of the wild-type AAV2 genome."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=a5d684b85c014f76a7b6d8e97945f0fb"
     CDS             3531..4391
                     /label=AmpiciIIin
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
     rep_origin      4562..5150
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgctg cagagggccc tgcgtatgag
      181 tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc
      241 ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga
      301 gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga
      361 cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc
      421 tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg
      481 ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg
      541 accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag
      601 gagtcgtgtc gtgcctgaga gcgcagcaag tttgtacaaa aaagcaggct ataacttcgt
      661 ataggatact ttatacgaag ttatcctcga gtaccggtca cgcgtacgca agcttgataa
      721 cttcgtatag catacattat acgaagttat cctagggcca ccttacagga tgatgcagca
      781 cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc
      841 cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc
      901 gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg
      961 gccgccgtcc ttcagcttca gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg
     1021 gtacatccgc tcggaggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc
     1081 ggaggggaag ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga
     1141 ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt
     1201 gaagccctcg gggaaggaca gcttcaagta gtcggggatg tcggcggggt gcttcacgta
     1261 ggccttggag ccgtacatga actgagggga caggatgtcc caggcgaagg gcagggggcc
     1321 acccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc
     1381 gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat
     1441 gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatgg tggctctaga
     1501 ataacttcgt ataaagtatc ctatacgaag ttataggtac ctgagctcaa catcgatagc
     1561 actagtataa cttcgtataa tgtatgctat acgaagttat acccagcttt cttgtacaaa
     1621 gtgggaattc cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1681 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1741 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     1801 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     1861 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     1921 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     1981 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     2041 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2101 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2161 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     2221 cgcatcggga attcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     2281 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     2341 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     2401 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc
     2461 tggggagggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     2521 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     2581 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     2641 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     2701 gcggcgcatt aagcgcggcg ggggtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2761 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2821 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2881 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     2941 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3001 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     3061 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3121 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     3181 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3241 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3301 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3361 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3421 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3481 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3541 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3601 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3661 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3721 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3781 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3841 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3901 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3961 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4021 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4081 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4141 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4201 cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc cgcggtatca
     4261 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4321 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4381 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4441 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4501 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4561 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4621 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4681 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4741 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4801 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4861 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4921 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4981 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5041 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5101 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5161 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc
     5221 ag
//
