LOCUS       pGH1327_(pGH1173_with_n 9166 bp ds-DNA     circular     06-APR-2022
DEFINITION  .
KEYWORDS    "marker:AmpR, HIV-1_5_LTR, HIV-1_5_LTR, PuroR"
FEATURES             Location/Qualifiers
     misc_feature    complement(196..205)
                     /label="GC Box"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    274..454
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1031..1037)
                     /label="TATA box"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1125..1358
                     /label="RRE"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1889..1903
                     /label="cPPT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2055..2085
                     /label="MCS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2113..3074
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3076..3285
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3298..3324
                     /label="HA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3325..3870
                     /label="PYL1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3325..3870
                     /label="Translation 3325-3870"
                     /translation="GGGAPTQDEFTQLSQSIAEFHTYQLGNGRCSSLLAQRIHAPPETVWSVVSRFDRPQIYKHFIKSCNVSEDFEMRVGCTRDVNVISGLPANTSRERLDLLDDDRRVTGFSITGGEHRLRNYKSVTTVHRFEKEEEEERIWTVVLESYVVDVPEGNSEEDTRLFADTVIRLNLQKLASITEAMN"
     CDS             3871..3900
                     /label="Translation 3871-3900"
                     /translation="SGGGGSGAAA"
     misc_feature    3901..3918
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3919..3939
                     /label="TypeIIS cloning site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3940..3948
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3949..4002
                     /label="T2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4003..4710
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4711..4719
                     /label="GSG linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4711..4776
                     /label="P2A(1)"
                     /ApEinfo_revcolor="#004040"
                     /ApEinfo_fwdcolor="#004040"
     CDS             4720..4776
                     /label="P2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4720..4776
                     /label="P2A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             4777..5376
                     /label="PuroR"
                     /ApEinfo_revcolor="#ca72ff"
                     /ApEinfo_fwdcolor="#ca72ff"
     misc_recomb     5396..5429
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    5475..6075
                     /label="wPRE"
                     /ApEinfo_revcolor="#0060ff"
                     /ApEinfo_fwdcolor="#0060ff"
     misc_feature    6265..6279
                     /label="cPPT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6602..6782
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(6826..7508)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_fwdcolor="#808080"
     misc_feature    complement(6829..7448)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(6853..6862)
                     /label="GC Box"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(7606..8265)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(8505..8533)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    8859..256
                     /label="pU6"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
       61 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      241 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      301 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      361 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      421 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      481 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      541 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      601 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      661 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      721 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      781 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      841 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      901 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      961 aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1021 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1081 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1141 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1201 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1261 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1321 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1381 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1441 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1501 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1561 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1621 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     1681 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     1741 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     1801 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     1861 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1921 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1981 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     2041 agtaccgggc ccgctctaga GATCCCATTT GACTCAGgcg gccgcgtgga taaccgtatt
     2101 accgccatgc atGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT
     2161 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG
     2221 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA
     2281 GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA
     2341 GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT
     2401 GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG
     2461 GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG
     2521 CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG
     2581 CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT
     2641 TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG
     2701 GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC
     2761 TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG
     2821 TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG
     2881 CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT
     2941 GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT
     3001 TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC
     3061 TCGATTAGTT CTCGcGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG
     3121 CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA
     3181 TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC
     3241 AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAgCTAgc caccatgTAT
     3301 CCCTATGACG TGCCCGATTA TGCCggtggg ggcgcgccaa ctcaagacga attcacccaa
     3361 ctctcccaat caatcgccga gttccacacg taccaactcg gtaacggccg ttgctcatct
     3421 ctcctagctc agcgaatcca cgcgccgccg gaaacagtat ggtccgtggt gagTcgtttc
     3481 gataggccac agatttacaa acacttcatc aaaagctgta acgtgagtga agatttcgag
     3541 atgcgagtgg gatgcacgcg cgacgtgaac gtgataagtg gattaccggc gaatacCtct
     3601 cgagagagat tagatctgtt ggacgatgat cggagagtga ctgggtttag tataaccggt
     3661 ggtgaacata ggctgaggaa ttataaatcg gttacgacgg ttcatagatt tgagaaagaa
     3721 gaagaagaag aaaggatctg gaccgttgtt ttggaatctt atgttgttga tgtaccggaa
     3781 ggtaattcgg aggaagatac gagattgttt gctgatacgg ttattagatt gaatcttcag
     3841 aaacttgctt cgatcactga agctatgaac AGCGGAGGAG GAGGTAGCgg agcggccgct
     3901 ggATCCggaT CAggctccgG AGACGGGtca AgCGTCTCtg gAtctgGAga gggAaggggA
     3961 agcctcctaa cttgcggaga Tgtcgaggag aatcctggcc caatggtgag caagggcgag
     4021 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     4081 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     4141 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     4201 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     4261 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     4321 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     4381 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     4441 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     4501 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     4561 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     4621 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     4681 tccaccggcg gcatggacga gctgtacaag GGAAGCGGAG CTACTAACTT CAGCCTGCTG
     4741 AAGCAGGCTG GTGACGTGGA GGAGAACCCT GGACCTATGA CCGAGTACAA GCCCACGGTG
     4801 CGCCTCGCCA CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC
     4861 GACTACCCCG CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG GGTCACCGAG
     4921 CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC
     4981 GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC
     5041 GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG
     5101 ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC
     5161 GGCGTATCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG
     5221 GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC
     5281 CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG
     5341 CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCtaagaat tcgtcgaggg acctaataac
     5401 ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt ccactaggta
     5461 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     5521 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5581 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5641 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5701 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5761 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5821 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5881 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5941 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6001 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6061 gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat
     6121 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     6181 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     6241 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6301 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     6361 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     6421 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     6481 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     6541 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     6601 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6661 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6721 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6781 cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7681 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7741 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7921 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8461 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8581 aaaagtgcca cctgacgctt aagcggtcga cggatcggga gatctcccga tcccctatgg
     8641 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     8701 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     8761 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     8821 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     8881 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     8941 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     9001 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     9061 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     9121 aatgacggta aatggcccgc ctggcattat gcccagtaca tgacct
//