LOCUS       pSL187_3X_NES_backbone  8716 bp ds-DNA     circular     28-FEB-2024
DEFINITION  .
KEYWORDS    "creator:Shane LF" "marker:AmpR, HIV-1_5_LTR, PuroR, HIV-1_5_LTR"
FEATURES             Location/Qualifiers
     misc_feature    complement(196..205)
                     /label="GC Box"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: GC Box note: label: GC Box"
     misc_feature    274..454
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: HIV-1_5_LTR note: label: HIV-1_5_LTR"
     misc_feature    complement(1031..1037)
                     /label="TATA box"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: TATA box note: label: TATA box"
     misc_feature    1125..1358
                     /label="RRE"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: RRE note: label: RRE"
     misc_feature    1889..1903
                     /label="cPPT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: cPPT note: label: cPPT"
     misc_feature    2055..2085
                     /label="MCS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: MCS note: label: MCS"
     misc_feature    2113..3074
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: EF1Alpha note: label: EF1Alpha"
     misc_feature    3076..3285
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: EF1Alpha note: label: EF1Alpha"
     CDS             3295..3468
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MYPYDVPDYAGSLQLPPLERLTLGSLQLPPLERLTLGSLQLPPLERLTLAAAGSGSGS"
     misc_feature    3298..3324
                     /label="HA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3337..3363
                     /label="NES"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="codon_start: 1 label: NES product: nuclear export signal from the HIV Rev protein (Fischer et al., 1995) transl_table: 1 translation: LPPLERLTL"
     CDS             3376..3402
                     /label="NES"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="codon_start: 1 label: NES product: nuclear export signal from the HIV Rev protein (Fischer et al., 1995) transl_table: 1 translation: LPPLERLTL"
     CDS             3415..3441
                     /label="NES"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="codon_start: 1 label: NES product: nuclear export signal from the HIV Rev protein (Fischer et al., 1995) transl_table: 1 translation: LPPLERLTL"
     misc_feature    3452..3468
                     /label="Linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: Linker note: label: Linker"
     misc_feature    3469..3489
                     /label="TypeIIS cloning site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: TypeIIS cloning site note: label: TypeIIS cloning site"
     misc_feature    3490..3498
                     /label="Linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: Linker note: label: Linker"
     misc_feature    3499..3552
                     /label="T2A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: T2A note: label: T2A"
     misc_feature    3553..3572
                     /label="pGH1332_sgRNA_target"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: pGH1332_sgRNA_target"
     CDS             3553..4260
                     /label="mCherry"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="label: mCherry note: codon_start: 1 label: mCherry transl_table: 1 translation: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     misc_feature    4261..4269
                     /label="GSG linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: GSG linker note: label: GSG linker"
     misc_feature    4261..4326
                     /label="P2A(1)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: P2A(1) note: label: P2A(1)"
     CDS             4270..4326
                     /label="P2A"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="label: P2A note: codon_start: 1 label: P2A transl_table: 1 translation: ATNFSLLKQAGDVEENPGP"
     misc_feature    4270..4326
                     /label="P2A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: P2A note: label: P2A"
     CDS             4327..4926
                     /label="PuroR"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="label: PuroR note: codon_start: 1 label: PuroR transl_table: 1 translation: MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_recomb     4946..4979
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: LoxP recombination site note: label: LoxP recombination site"
     misc_feature    5025..5625
                     /label="wPRE"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: wPRE note: label: wPRE"
     misc_feature    5815..5829
                     /label="cPPT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: cPPT note: label: cPPT"
     misc_feature    6152..6332
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: HIV-1_5_LTR note: label: HIV-1_5_LTR"
     rep_origin      complement(6376..7058)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT label: ColE1 origin note: direction: LEFT label: ColE1 origin"
     misc_feature    complement(6379..6998)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: pBR322_origin note: label: pBR322_origin"
     misc_feature    complement(6403..6412)
                     /label="GC Box"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: GC Box note: label: GC Box"
     CDS             complement(7156..7815)
                     /label="AmpR"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="label: AmpR note: codon_start: 1 label: AmpR transl_table: 1 translation: MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     misc_feature    complement(8055..8083)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: AmpR promoter note: label: AmpR promoter"
     misc_feature    8409..256
                     /label="pU6"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: pU6 note: label: pU6"
ORIGIN
        1 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
       61 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      241 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      301 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      361 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      421 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      481 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      541 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      601 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      661 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      721 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      781 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      841 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      901 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      961 aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1021 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1081 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1141 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1201 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1261 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1321 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1381 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1441 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1501 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1561 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1621 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     1681 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     1741 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     1801 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     1861 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1921 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1981 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     2041 agtaccgggc ccgctctaga GATCCCATTT GACTCAGgcg gccgcgtgga taaccgtatt
     2101 accgccatgc atGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT
     2161 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG
     2221 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA
     2281 GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA
     2341 GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT
     2401 GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG
     2461 GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG
     2521 CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG
     2581 CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT
     2641 TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG
     2701 GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC
     2761 TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG
     2821 TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG
     2881 CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT
     2941 GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT
     3001 TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC
     3061 TCGATTAGTT CTCGcGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG
     3121 CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA
     3181 TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC
     3241 AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAgCTAGc caccatgTAT
     3301 CCCTATGACG TGCCCGATTA TGCCggctca cttcaactgc ctccacttga aagactgaca
     3361 ctgggatcat tacaattacc tcctttagaa cgattaacac tcggttcact acagcttccg
     3421 cctcttgaga gattgacatt agcggccgct ggATCCggaT CAggctccgG AGACGGGtca
     3481 AgCGTCTCtg gAtctgGAga gggAaggggA agcctcctaa cttgcggaga Tgtcgaggag
     3541 aatcctggcc caatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     3601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     3661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     3721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     3781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     3841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     3901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     4021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     4081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     4141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     4201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     4261 GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG GTGACGTGGA GGAGAACCCT
     4321 GGACCTATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCCGG
     4381 GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAC
     4441 CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG
     4501 CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG
     4561 CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG
     4621 AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC
     4681 AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTATCGC CCGACCACCA GGGCAAGGGT
     4741 CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC
     4801 TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC
     4861 ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT
     4921 GCCtaagaat tcgtcgaggg acctaataac ttcgtatagc atacattata cgaagttata
     4981 catgtttaag ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa
     5041 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5101 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5161 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5221 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5281 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5341 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5401 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5461 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5521 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5581 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     5641 cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     5701 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     5761 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5821 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     5881 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     5941 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     6001 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     6061 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     6121 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     6181 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6241 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6301 acccttttag tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc
     6361 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6421 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6481 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6541 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6601 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6661 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6721 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6781 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6841 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6901 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6961 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7021 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7081 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7141 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7201 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7261 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7321 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7381 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7441 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7501 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7561 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7621 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7681 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7741 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7801 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7861 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7921 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7981 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8041 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8101 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgctt aagcggtcga
     8161 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     8221 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     8281 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     8341 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     8401 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     8461 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     8521 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     8581 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     8641 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     8701 gcccagtaca tgacct
//