LOCUS       mCh-CRY2-sspB(A5        6602 bp DNA     circular SYN 09-JUL-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    mCh-CRY2-MACF
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2024 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             606..1310
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             1335..2828
                     /codon_start=1
                     /label=CRY2
                     /note="color: #993366"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRACSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             2835..2906
                     /codon_start=1
                     /label=(SGGGGGGG)x3
                     /note="color: #00ff00"
                     /translation="SGGGGGGGSGGGGGGGSGGGGGGG"
     CDS             2919..3248
                     /codon_start=1
                     /label=sspb
                     /note="color: #993366"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASFTGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    3390..3511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(3518..3973)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4000..4104
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4106..4463
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4314..4449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             4498..5292
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5524..5571
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     rep_origin      5900..6488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcggtg
      601 gaatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
      661 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      721 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
      781 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
      841 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      901 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      961 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     1021 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     1081 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     1141 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     1201 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     1261 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtagcggac
     1321 caccggtcgc caccatgaag atggacaaaa agaccatcgt ctggtttcgg agagatttga
     1381 gaatagaaga taatcccgcg ctcgccgccg cggcccacga gggttccgtc ttccccgttt
     1441 tcatttggtg tcctgaagaa gaaggccagt tttatcccgg aagggcctct aggtggtgga
     1501 tgaagcaaag tctggcccat cttagccagt cactgaaagc actgggcagt gatcttaccc
     1561 tgatcaagac acacaatacc atctctgcca tcctcgactg catcagggtg accggcgcaa
     1621 cgaaagtcgt gtttaaccac ctgtacgatc cagttagtct ggtgcgcgac cacactgtga
     1681 aggagaagct ggtggaacgg gggatcagtg tgcagagcta caacggggac cttctgtacg
     1741 agccatggga gatctattgc gagaaaggga aaccgttcac ctccttcaac agttactgga
     1801 agaaatgttt ggatatgtca atagagtccg ttatgttgcc ccctccctgg agactgatgc
     1861 cgattactgc tgctgcagag gccatctggg cctgctccat cgaggaactc ggtctggaaa
     1921 atgaagcaga aaagccaagc aatgcacttc tcactagagc ctgtagcccc ggctggtcta
     1981 atgccgacaa gctgcttaac gagttcatcg aaaaacaact gattgactac gcgaagaact
     2041 ccaagaaagt ggtaggtaac tcaactagct tgctctctcc atatctccat tttggcgaga
     2101 tttctgtccg ccatgtattt cagtgcgctc ggatgaaaca gattatctgg gctcgcgata
     2161 aaaacagcga aggcgaagaa agcgccgatc tgttcctgcg agggatcgga cttcgggaat
     2221 actcccggta tatatgtttc aactttccat tcacacacga gcagagtctg ttgtcccacc
     2281 tcaggttctt cccctgggac gccgatgtcg acaaattcaa ggcatggaga cagggaagga
     2341 cagggtaccc actcgtggat gctggcatga gagagctctg ggctacaggc tggatgcaca
     2401 accgcatccg ggtaatcgtg tcctcatttg ctgtcaagtt tctgctcctg ccttggaaat
     2461 ggggaatgaa gtacttttgg gatacccttc tcgacgccga cttggagtgt gacattctgg
     2521 gatggcaata tattagcggg tcaattcctg acggccatga gttggacagg ttggacaatc
     2581 cggccttgca gggagctaag tatgatcccg aaggagagta tattcgacag tggctccccg
     2641 agctggcccg acttcctacg gagtggattc accatccttg ggacgcacca ctgacagtgc
     2701 tcaaggcaag cggggtggag ctgggcacca attacgctaa gcctatagtt gatatagata
     2761 cagcacgcga gctgctggct aaagcgatct ctcgcactcg ggaggcgcag attatgatcg
     2821 gtgctgccgt gtacagtggg ggaggcggag ggggtggttc aggtggtggt ggtggtggtg
     2881 gttcgggtgg tggcggaggg ggaggctccg gaggtagtag ctccccgaaa cgccctaagc
     2941 tgctgcgtga atattacgat tggctggttg ataacagctt taccccatat ctggtggtgg
     3001 atgccacata cctgggcgtg aacgtgcccg tggagtatgt gaaagacggt cagatcgtgc
     3061 tgaatctgtc tgcaagtttt accggcaacc tgcaactgac aaatgatttt atccagttca
     3121 acgcccgctt taagggcgtg tctcgtgaac tgtatatccc gatgggtgcc gctctggcca
     3181 tttacgctcg cgagaacggc gatggtgtga tgttcgaacc agaagaaatc tatgacgagc
     3241 tgaatattta agcggccgcg ggatccaccg gatctagata actgatcata atcagccata
     3301 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3361 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3421 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3481 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
     3541 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3601 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3661 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3721 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3781 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3841 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3901 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3961 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4021 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4081 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     4141 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4201 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4261 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4321 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4381 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4441 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     4501 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4561 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4621 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     4681 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4741 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4801 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4861 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4921 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4981 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     5041 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5101 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5161 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5221 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5281 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5341 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5401 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5461 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     5521 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     5581 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     5641 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     5701 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     5761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6481 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6541 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     6601 at
//
